Rhizosphere最新文献

{"title":"Soybean rhizosphere communities are shaped more by cropping systems than by Bacillus subtilis delivery methods","authors":"Maria Clara Zerbinatti ,&nbsp;Élida Moreira L. Santana ,&nbsp;Marcela Fernanda S. Martins ,&nbsp;Fábio Fernando Araújo ,&nbsp;Lucas W. Mendes ,&nbsp;Romário M. Costa ,&nbsp;Ademir Sergio F. Araujo","doi":"10.1016/j.rhisph.2025.101199","DOIUrl":"10.1016/j.rhisph.2025.101199","url":null,"abstract":"<div><div>Agricultural management systems, such as crop-livestock integration and fallow, affect differently soil properties, mainly biological parameters. This can drive the rhizosphere microbiome. In addition, methods of microbial inoculation can affect the rhizosphere. This study aims to investigate the effects of inoculating <em>Bacillus subtilis</em> under different methods (furrow, soil surface, and via organic compost), comparing soybean grown under crop-livestock integration and fallow and their effect on the prokaryotic communities in the rhizosphere. Soybean was grown under these agricultural systems and the prokaryotic communities in the rhizosphere was assessed by 16S rRNA sequencing. The prokaryotic communities in the rhizosphere of soybean differed significantly between crop-livestock and fallow (PERMANOVA <em>p</em> &lt; 0.001), while inoculation methods with <em>B. subtilis</em> had minimal influence. Both systems shaped distinctly the structure of prokaryotic communities, with lower richness and diversity in fallow without inoculation. Actinobacteriota (∼50 %), Proteobacteria (∼15 %), and Firmicutes (∼10 %) dominated, with Proteobacteria more abundant in uninoculated soybean under fallow, and Firmicutes more prevalent in uninoculated soybean under crop-livestock. Soybean grown under crop-livestock enriched specific taxa, such as <em>Bacillus</em> and <em>Bradyrhizobium</em>, while soybean under fallow enriched <em>Streptomyces</em> and <em>Gaiella</em>. Niche analysis showed similar specialists comparing fallow and crop-livestock. In conclusion, crop-livestock shaped a more diverse soybean rhizosphere microbiome than fallow, with minimal influence from inoculation methods.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"36 ","pages":"Article 101199"},"PeriodicalIF":3.5,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145268073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Herbaspirillum seropedicae inoculation alters maize root metabolism, rhizosphere pH, and seed-resident bacteriome composition","authors":"Luiz Eduardo Souza da Silva Irineu ,&nbsp;Cleiton de Paula Soares ,&nbsp;Priscila Pires Bittencourt ,&nbsp;Leticia Cespom Passos ,&nbsp;Sávio Bastos de Souza ,&nbsp;Luiz Fernando Wurdig Roesch ,&nbsp;Arnoldo Rocha Façanha ,&nbsp;Fabio Lopes Olivares","doi":"10.1016/j.rhisph.2025.101206","DOIUrl":"10.1016/j.rhisph.2025.101206","url":null,"abstract":"<div><div><em>Herbaspirillum seropedicae</em> is a plant growth-promoting bacterium that senses root exudates, colonizes the rhizosphere, attaches to the root surface and establishes endophytically in cereals. However, how these events reshape host physiological responses of maize roots and early microbiome assembly remains unclear. We investigated the metabolic and physiological responses of maize roots to inoculation with <em>H. seropedicae</em> HRC54 and assessed the consequences for the seed-resident/root-associated bacteriome. Gene expression of tricarboxylic acid (TCA) cycle enzymes was quantified by RT-qPCR. Rhizospheric H⁺ fluxes and surface pH were mapped using the non-invasive scanning ion-selective electrode technique (SIET). Root-zone attachment was visualized using scanning electron microscopy (SEM), and the bacterial community composition was profiled through 16S rRNA sequencing. Inoculation upregulated transcripts for aconitase, citrate synthase, isocitrate dehydrogenase, and succinate-CoA ligase, while downregulating fumarase and malate dehydrogenase. H⁺ efflux increased in the elongation zone and decreased in the root cap and root-hair zones, corresponding to localized pH shifts. SEM revealed preferential colonization of the elongation zone, matching localized pH shifts. SEM showed preferential colonization of the elongation zone, progressing from aggregates to biofilm within 24 h. Microbiome analysis revealed distinct beta-diversity and enrichment of genera such as <em>Aurantimonas</em>, <em>Mesorhizobium</em>, <em>Novosphingobium</em>, <em>Serratia</em>, and <em>Stenotrophomonas</em>, as well as a reduced abundance of several genera, including <em>Bradyrhizobium</em>, <em>Burkholderia</em>, and <em>Gluconacetobacter</em>. These results link TCA reprogramming to pH modulation and early microbiome reshaping, supporting seed-treatment strategies that enhance nutrient uptake, bolster resilience against root pathogens, and improve crop performance.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"36 ","pages":"Article 101206"},"PeriodicalIF":3.5,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145267535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maize landraces under water deficit favor diverse rhizosphere communities associated with improved stress response","authors":"Giulia Castorina ,&nbsp;Alessia Follador ,&nbsp;Martina Ghidoli ,&nbsp;Patrizia Zaccheo ,&nbsp;Laura Crippa ,&nbsp;Fulvia Tambone ,&nbsp;Alessandro Passera ,&nbsp;Carlotta Balconi ,&nbsp;Gabriella Consonni ,&nbsp;Paola Casati","doi":"10.1016/j.rhisph.2025.101200","DOIUrl":"10.1016/j.rhisph.2025.101200","url":null,"abstract":"<div><div>Climate change is intensifying water scarcity, posing major challenges to global crop productivity. Improving tolerance to limited water availability is therefore a key agricultural priority. While elite genotypes are widely used in breeding, maize landraces represent an underexploited reservoir of adaptive traits. Their interaction with soil microbial communities may play an important role in stress resilience that needs further investigation to have its extent fully understood. In this study, we investigated the rhizosphere microbiota of four maize landraces from Lombardy (Northern Italy) to assess how soil origin, plant genotype, and water availability interact in shaping bacterial communities. Plants were cultivated in soils collected from four locations, first under well-watered conditions and then exposed to water deficit. Growth and photosynthetic traits were monitored in parallel to link microbial composition with plant performance. Under well-watered conditions, rhizosphere communities were strongly shaped by the soil–genotype combination, with consistent enrichment of <em>Bacillota</em> taxa. Under water deficit, however, most landraces/soil combinations exhibited a reduced rhizosphere effect that brought rhizosphere bacterial communities to become indistinguishable from bulk soil. Strikingly, landraces displaying the best tolerance to water deficit showed an increase of biodiversity in the rhizosphere bacterial community, suggesting a recruitment strategy opposing that shown in well-watered conditions. These results highlight the importance of integrating landrace diversity and microbiome interactions into strategies for improving maize resilience. The study demonstrates that not only soil and genotype, but also the capacity to sustain distinctive and diverse microbial associations under stress, may contribute to plant performance in water-limited environments.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"36 ","pages":"Article 101200"},"PeriodicalIF":3.5,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145267531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Key bacterial players in the growth of Kandelia obovata: Insights from rhizosphere soil composition","authors":"Shouji Gong ,&nbsp;Xiaokui Xie ,&nbsp;Riming Wang ,&nbsp;Xiujian Li","doi":"10.1016/j.rhisph.2025.101196","DOIUrl":"10.1016/j.rhisph.2025.101196","url":null,"abstract":"<div><div>The structure of soil bacterial communities within mangrove forests has received widespread attention, which can promote the growth of mangroves and material transformation. Despite the recognized significance of mangrove ecosystems, the bacterial composition within artificially restored mangroves remains poorly understood. This study investigates the bacterial diversity in the rhizosphere soil of both naturally and artificially restored <em>Kandelia obovata</em> seedlings, utilizing Illumina NovaSeq high-throughput sequencing technologies. The results revealed that only a small fraction of bacteria were identified in the mangrove rhizosphere soil, with the majority of them remaining uncharacterized. The dominant bacterial taxa identified included <em>Sulfurovum</em>, <em>Actibacter</em>, <em>Woeseia</em>, <em>Desulfatiglans</em>, <em>Halioglobus</em>, <em>Ignavibacterium</em>, <em>Spirochaeta</em>, <em>Sulfurimonas, Prolixibacter</em>, <em>Robiginitalea</em>, and <em>Algoriphagus</em>. Furthermore, marked differences were noted in the abundance of <em>Sulfurovum</em>, <em>Actibacter</em>, <em>Woeseia</em>, <em>Desulfatiglans</em>, <em>Halioglobus</em>, <em>Methanosaeta</em> and <em>Robiginitalea</em> between natural and artificially restored <em>Kandelia obovata</em> seedlings. Spatial network analysis suggested that <em>Sulfurovum</em>, <em>Actibacter</em>, <em>Ignavibacterium</em>, and <em>Desulfatiglans</em> may play important roles in the growth process of <em>Kandelia obovata</em> and are potentially important bacteria for its development. These findlings enhance our understanding of bacterial community structure in mangroves and underscore the potential application of bacteria in mangrove restoration.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"36 ","pages":"Article 101196"},"PeriodicalIF":3.5,"publicationDate":"2025-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145267533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potassium salt of 1-naphthaleneacetic acid promotes rhizogenesis in Amsonia elliptica stem cuttings","authors":"Youn Hwa Son ,&nbsp;Suejin Park ,&nbsp;Chung Ho Ko ,&nbsp;Mengmeng Gu ,&nbsp;Seung Youn Lee","doi":"10.1016/j.rhisph.2025.101203","DOIUrl":"10.1016/j.rhisph.2025.101203","url":null,"abstract":"<div><div><em>Amsonia elliptica</em> is valued for its medicinal and ornamental potential. However, it is listed as an endangered species in South Korea due to overharvesting and habitat destruction. This study aimed to develop an efficient protocol for mass vegetative propagation of <em>A. elliptica</em> using stem cuttings. We tested Rootone (0.4 % 1-naphthylacetic acid) and potassium salts of indole-3-butyric acid (K-IBA) and 1-naphthaleneacetic acid (K-NAA) applied via basal dip or foliar spray. After six weeks, cuttings treated with 2000 mg L⁻¹ K-NAA showed the best rooting traits, with 93.3 % rooting, 17.5 roots per cutting, and 55.4 mm root length, along with the highest fresh (92.9 mg) and dry root mass (8.8 mg). Dipping cuttings in 2000 mg L⁻¹ K-NAA for 1 min is recommended as the most effective treatment for large-scale propagation of <em>A. elliptica</em>. This optimized propagation method will facilitate the conservation and ornamental use of <em>A. elliptica</em>.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"36 ","pages":"Article 101203"},"PeriodicalIF":3.5,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145221128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Co-variation between bacterial genome traits and root functional traits influences soil multifunctionality of five temperate tree species","authors":"Chunhua Lv ,&nbsp;Ying Jin ,&nbsp;Yiling Li ,&nbsp;Zhenghu Zhou","doi":"10.1016/j.rhisph.2025.101202","DOIUrl":"10.1016/j.rhisph.2025.101202","url":null,"abstract":"<div><div>Microbial genomic traits reflect microbial community responses and adaptations to resource and stress variations. However, less is known about how the co-variation between bacterial genome traits and root functional traits affects soil multifunctionality (i.e., the soil's capacity to support multiple ecological functions and services simultaneously) in response to drought. Here, we conducted a short-term rainfall exclusion experiment in a common garden with five temperate tree species in northeast China. Our results revealed rhizosphere bacteria associated with foraging roots (with high specific root length and area) exhibited larger genome size, a higher number of coding genes, elevated GC content, and increased body size. These root and bacterial traits were also positively correlated with high rhizosphere soil multifunctionality. In addition, foraging roots were linked to stronger rhizosphere effects on bacterial traits, while both root foraging capacity and energy reserves (soluble sugars and starch) were positively associated with rhizosphere effects on soil multifunctionality. Drought increased bacterial genome size, the number of coding genes, GC content, and the oligotroph/copiotroph ratio, while bacterial traits and soil multifunctionality in both the rhizosphere and bulk soils exhibited synchronous responses to drought. In summary, our study suggests that co-variation between bacterial genomes and root functional traits has critical regulation on soil function and rhizosphere effects.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"36 ","pages":"Article 101202"},"PeriodicalIF":3.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145267530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbiome-mediated rhizosphere engineering for the alleviation of drought stress: A promising strategy for agricultural sustainability","authors":"Argha Sinha,&nbsp;Mohita Nigam,&nbsp;Shilpi Sharma","doi":"10.1016/j.rhisph.2025.101201","DOIUrl":"10.1016/j.rhisph.2025.101201","url":null,"abstract":"<div><div>The necessity to effectively manage drought stress in agriculture is becoming more fundamental as climate change continues to compromise global food security. In plants, drought vitiates their osmotic balance, nutrient acquisition, and photosynthetic performance. Recent advances show that microbiome-mediated rhizosphere engineering can buffer these effects by activating defined physiological pathways in the host. Beneficial soil-dwelling microbes living in close association with roots can enhance drought tolerance via ABA-arbitrated stomatal regulation, osmolyte accumulation, antioxidant enzyme induction, aquaporin-mediated water transport modulation, and root system architecture restructuring. Rhizosphere engineering possesses excellent potential to bypass the limitations associated with conventional bioformulations such as limited persistence and competitive interactions with native microbes. Two complementary strategies dominate this field: designing synthetic microbial communities (SMCs), which stacks functional traits for stabilizing water-use efficiency, and host-mediated microbiome engineering (HMME), which selects adaptive communities through iterative host-driven filtering. While controlled investigations corroborate these processes, translation to field conditions is stalled by ecological variability and lack of systematic trial designs. Moving forward, progress will require systematic mapping of microbial functions to plant drought-response pathways using multi-omics. Besides, there is a need for development of hybrid pipelines that combine top-down and bottom-up microbiome engineering, standardized bioinoculant formulation, delivery, and persistence tracking, and long-term, multi-site validation under realistic agronomic scenarios. In this review, we synthesize mechanistic insights, benchmark current approaches, and outline practical roadmaps for scaling microbiome-based drought mitigation techniques towards producing resilient, climate-smart crops. Utilizing acclimatizable plant-microbiome interactions is of utmost importance for ushering in agricultural sustainability in water-scarce environments.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"36 ","pages":"Article 101201"},"PeriodicalIF":3.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145268074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Root border cells within mucilage: the ‘mucicell’ concept for rhizosphere functions","authors":"Meisam Nazari ,&nbsp;Asma Fathinejad ,&nbsp;Mohammad Hossein Mohammadi ,&nbsp;Frédéric Lamblin ,&nbsp;Yakov Kuzyakov","doi":"10.1016/j.rhisph.2025.101197","DOIUrl":"10.1016/j.rhisph.2025.101197","url":null,"abstract":"<div><div>Root mucilage plays a pivotal role in mediating plant-soil interactions, influencing rhizosphere physical, biochemical, hydraulic, and microbial functions. Current studies frequently overlook the consistent co-occurrence and functions of root cap border cells within root mucilage, as confirmed through microscopic observations. This oversight may lead to misinterpretations of isolated effects of ‘pure’ mucilage. Here, we propose the concept of the ‘mucicell’, a composite functional unit comprising mucilage and its embedded, metabolically active border cells, as an accurate framework to describe the biological material and its functions within the rhizosphere. This opinion article critically re-evaluates key rhizosphere functions, including its hydrophobicity after drying and subsequent rewetting, water retention, and microbial dynamics, all affected by root border cells within mucilage. We argue that many effects traditionally attributed to mucilage alone likely arise from the combined activity of the mucicell complex. The unique biochemical and biophysical attributes of border cells are shown to modulate the properties of mucilage, thereby influencing plant-soil interactions in previously unrecognized ways. Adopting the mucicell concept offers a holistic understanding of rhizodeposition and its role in shaping the rhizosphere functions, with important implications for experimental design and data interpretation.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"36 ","pages":"Article 101197"},"PeriodicalIF":3.5,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145267534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactive plant secondary metabolite production enhanced by AMF choice in mixed inoculum","authors":"Caio Bezerra Barreto ,&nbsp;Rupam Kapoor ,&nbsp;Qiang-Sheng Wu ,&nbsp;Mohamed Hijri ,&nbsp;Odair Alberton ,&nbsp;Carmelo José Albanez Bastos-Filho ,&nbsp;Michele Dalvina Correia da Silva ,&nbsp;Fábio Sérgio Barbosa da Silva","doi":"10.1016/j.rhisph.2025.101198","DOIUrl":"10.1016/j.rhisph.2025.101198","url":null,"abstract":"<div><div>Flavonoids are among the plant bioactive compounds that may have their production enhanced by arbuscular mycorrhizal fungi (AMF). These antioxidant bioactive compounds are related to plant Sun Protection Factor (SPF), which is relevant to plant-based cosmetic formulations. Although it is known that enhancing the rhizosphere with AMF isolates improves the SPF, the role of AMF consortia in affecting plant SPF is not yet established. The study aimed to select the most efficient mycorrhizal consortium to optimize the production of foliar bioactive compounds, antioxidant activity, and the associated <em>in vitro</em> SPF of <em>Schinus terebinthifolia</em> Raddi seedlings. A randomized experiment was conducted in a greenhouse, with a non-inoculated control and with AMF inoculation of <em>Acaulospora longula</em>, <em>Dentiscutata heterogama</em> and <em>Entrophospora etunicata</em> inoculation, singly or in consortia. After 191 days, roots were collected to evaluate the mycorrhizal colonization, and leaves were analyzed for photosynthetic pigments, the antioxidant activity, flavonoids, flavonols, and proanthocyanidins content, compounds related to SPF. Allometric parameters were also evaluated. Some selected consortia significantly improved the SPF, phytomass accumulation, and the production of photosynthetic pigments, flavonoids, and proanthocyanidins, with increases reaching up to 200% to control plants. Antioxidant activity was enhanced by approximately 110% due to mycorrhizal inoculation. This study is the first to elucidate the role of AM consortia in modulating the SPF and phytochemistry in leaves of <em>S. terebinthifolia</em> seedlings. The use of consortium containing <em>A. longula</em>, <em>D. heterogama</em> and <em>E. etunicata</em> is recommended to increase the content of bioactive phenolics, antioxidants, and photoprotective activities in <em>S. terebinthifolia</em> phytomass.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"36 ","pages":"Article 101198"},"PeriodicalIF":3.5,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145267532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient colonization of pecan (Carya illinoinensis) seedlings by Tuber floridanum","authors":"Joice Aline Freiberg ,&nbsp;Tine Grebenc ,&nbsp;Rafael Marian Callegaro ,&nbsp;Zaida Inês Antoniolli","doi":"10.1016/j.rhisph.2025.101195","DOIUrl":"10.1016/j.rhisph.2025.101195","url":null,"abstract":"<div><div>The occurrence of the truffle <em>Tuber floridanum</em> in pecan orchards (<em>Carya illinoinensis</em> Wangenh. K. Koch) in southern Brazil represents a potential new income alternative for pecan farmers. However, little is known regarding the nutritional and morphological parameters of pecan seedlings inoculated with this truffle species. Therefore, we aimed to quantify the ectomycorrhizal association between <em>T. floridanum</em> and pecan seedlings, and to evaluate whether this symbiosis promotes the growth under greenhouse conditions. Pecan seedlings, cultivar Barton, were inoculated with <em>T. floridanum</em>, and after 300 days in the greenhouse, the percentage of mycorrhization, nutritional and morphological growth parameters were evaluated. Our results indicate high levels of <em>T. floridanum</em> colonization in pecan seedlings (78.7 %–99.5 %) after ten months of inoculation. Further studies are recommended to evaluate the long-term effect of this ectomycorrhizal association, particularly under extended cultivation periods and conditions of nutritional stress.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"36 ","pages":"Article 101195"},"PeriodicalIF":3.5,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145221687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}