Rhizosphere最新文献

{"title":"Symbiotic N2 fixation in cowpea varieties is markedly enhanced by inoculation with elite Bradyrhizobium strains","authors":"Tewodros Ayalew ,&nbsp;Tarekegn Yoseph ,&nbsp;Georg Cadisch","doi":"10.1016/j.rhisph.2024.100976","DOIUrl":"10.1016/j.rhisph.2024.100976","url":null,"abstract":"<div><div>Because of its excellent ability to fix atmospheric nitrogen, cowpea [<em>Vigna unguiculata</em> (L.) Walp] makes a significant contribution to soil sustainability and productivity in the resource limited tropical regions. However, due to in part to ineffectiveness and limited availability of bio-inoculant, its symbiotic N contribution and yield remained low in the field. Therefore, this study examined the effect of elite cowpea infecting <em>Bradyrhizobium</em> strains (CP-24 and CP-37) on shoot biomass and symbiotic nitrogen contributions of four cowpea varieties (Keti, TVU, Black eye bean, and White wonderer trailing). For this a two-year field experiment was carried out at three sites using a factorial randomized complete block design with four replications. The natural abundance of the ¹⁵N technique was used to compute the symbiotic N contribution. <em>Bradyrhizobium</em> inoculation led to significantly higher nodule formation, % Ndfa, amounts of N fixed, and shoot biomass, demonstrating the effectiveness and ability of the strains to enhance soil fertility. Inoculating cowpea with CP-24 strain increased shoot N content, % Ndfa and N fixed by 40%, 15%, and 41%, respectively, in comparison to the un-inoculated control. Furthermore, the inoculant by variety interaction had a significant effect on nodule number, nodule dry weight, and amount of N fixed, with TVU and White Wonderer trailing in combination with CP-24 exhibiting the most outstanding performance. There was also a strong positive correlation between biomass accumulation and N fixed, as well as N fixed and seed yield. Therefore, <em>Bradyrhizobium</em> inoculation on cowpea varieties TVU and White Wonderer trailing with CP-24 strain is recommended at all three tested sites and similar agro-ecologies for improved symbiotic N contribution and associated yield advantage of cowpea. This study highlights that, the use of elite and crop specific <em>Bradyrhizobium</em> strains can boost symbiotic nitrogen contribution, soil fertility, and the yield performance of legumes. Thus, it helps resource-poor farmers who are suffering from rising mineral fertilizer cost to achieve food security while reducing climate change risks.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142446690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three-dimensional numerical modeling of soil-roots system based on X-ray computed tomography: Hydraulic effects study","authors":"Ji-Peng Wang ,&nbsp;Jun-Feng Sha ,&nbsp;Shangqi Ge ,&nbsp;Xu-Guang Gao ,&nbsp;Abdelali Dadda","doi":"10.1016/j.rhisph.2024.100975","DOIUrl":"10.1016/j.rhisph.2024.100975","url":null,"abstract":"<div><div>Vegetation roots enhance soil stability by influencing saturation and pore structure, playing a pivotal role in stabilizing slopes, reducing erosion, and enhancing soil structure. However, current research on the hydraulic effects of roots on soil remains relatively limited. The micro-mechanisms of vegetation's impact on soil and the macro-level connections are not yet fully understood, which poses a challenge to the modeling of root-soil system. This study develops a three-dimensional (3D) finite element model of root-soil composites based on root computed tomography (CT) images and experimental results. Four different groups are modeled, including the rootless group, and those with <em>Festuca arundinacea</em> (FA) roots at various growth stages. The simulation results show that the saturation in the shallow layers significantly decreases in root-soil composite groups, and the rhizosphere water content is lower than that away from the roots, resulting in a net water flux toward the roots. The influence range of roots on suction is gradually amplified with increasing root growth process and root water uptake time. Higher levels of root development result in a stronger overall water uptake effect, leading to a more pronounced decrease in saturation. Closer proximity to the surface roots results in a more rapid increase in soil suction. Compared with one-dimensional root water uptake models, this model considers the effects of spatial heterogeneity of root structures on soil, which provides a comprehensive modeling basis for studying the effect of root system on soil.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142528594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced soil stabilisation and growth of Lolium perenne through combined seeding with Cynodon dactylon","authors":"Hao Gu ,&nbsp;Yuan Wang ,&nbsp;Sheng Liu ,&nbsp;Haikuan Chen ,&nbsp;Lu Jia ,&nbsp;Zhongyuan Chen","doi":"10.1016/j.rhisph.2024.100977","DOIUrl":"10.1016/j.rhisph.2024.100977","url":null,"abstract":"<div><div>Herbaceous plants play a crucial role in soil stabilisation, and combined seeding is often employed in ecosystem management to promote biodiversity. This study investigated the influence of combined seeding of Ryegrass (<em>Lolium perenne</em>) and Bermuda (<em>Cynodon dactylon</em>) on plant growth and soil stabilisation through in-situ sampling and indoor experimental measurements. Four experimental plots were established: a. bare soil, b. <em>L. perenne</em> single species, c. <em>C. dactylon</em> single species, d. combined <em>L. perenne</em> and <em>C. dactylon</em>. The results indicate that combined seeding inhibited the development of <em>L</em>. <em>perenne</em> and <em>C</em>. <em>dactylon</em> root depth by 14.50% and 29.20%, respectively. However, shoot height, total leaf area, total root length and total root surface area increased in <em>L</em>. <em>perenne</em> under combined seeding, while these parameters decreased for <em>C</em>. <em>dactylon</em>. Combined seeding significantly enhanced the resistance to breakage in tension and tensile strength of <em>L</em>. <em>perenne</em>, with no significant impact on <em>C</em>. <em>dactylon</em>. Plant roots notably increased soil cohesion, with a respective increase of 37.08%, 26.98%, and 50.81% in cohesion for <em>L. perenne</em> single species plot, <em>C. dactylon</em> single species plot, and combined <em>L. perenne</em> and <em>C. dactylon</em> plot compared to bare soil. The root content in combined seeding significantly increased, with an increase of 27.17% and 65.20% compared to single seeding of <em>L</em>. <em>perenne</em> and <em>C</em>. <em>dactylon</em>, respectively. Additionally, under the influence of roots, the soil moisture content in the combined seeding plot was lower than in the single species and bare soil plots. These findings highlight that combined seeding enhanced plant competition, improved soil shear strength, and provided significant ecological benefits, offering insights for vegetation-based slope design.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142446952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revealing the characteristics of myxobacterial communities in rhizosphere and non-rhizosphere soils of halophytic plants following wetland degradation using high-throughput sequencing","authors":"Cheng Ding ,&nbsp;Wenge Hu ,&nbsp;Xiaoyun Qi,&nbsp;Suhui Hou,&nbsp;Yang Li,&nbsp;Jie Xiong,&nbsp;Ting Chen,&nbsp;Xue Zhang","doi":"10.1016/j.rhisph.2024.100972","DOIUrl":"10.1016/j.rhisph.2024.100972","url":null,"abstract":"<div><div>Myxobacteria represent a distinct group in soil microbial communities, with their predatory capabilities playing a vital role in regulating and stabilizing these communities. However, the characteristics of myxobacterial communities in the soils of Karelinia caspia (KC), a halophytic plant growing in degraded wetlands, remain unclear. In this study, we investigated myxobacterial communities in rhizosphere and non-rhizosphere soils from nine KC’ sampling sites in saline-alkaline land formed after wetland degradation using high-throughput sequencing. A total of 486 myxobacterial ASVs were identified from both rhizosphere and non-rhizosphere soil samples, comprising 2.68% of the total bacterial community. The dominant genera at the genus level were <em>Unclassified_g_bacteriap25</em> and <em>Haliangium</em>. FAPROTAX functional predictions indicated that myxobacteria in rhizosphere soils performed various ecological functions, such as predation, organic matter decomposition, and cellulose degradation, while in non-rhizosphere soils, they primarily displayed predatory functions. The high proportion of unclassified functions suggests that many aspects of myxobacteria in soil remain unexplored. Correlation analysis showed that electrical conductivity, soil moisture, and total nitrogen significantly affected myxobacterial diversity and abundance. Co-occurrence network analysis revealed multiple associations between myxobacteria and other bacterial groups, highlighting their crucial role in maintaining the dynamic equilibrium of soil bacterial communities. These findings deepen our understanding of the relationship between microbial community dynamics and environmental factors, laying a strong foundation for future research in soil microbial ecology and conservation strategies.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142528726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rhizophagus intraradices symbiosis with Amaranthus hypochondriacus improves rhizosphere soil pH and nutrient status in sodic soil","authors":"Honghe Li ,&nbsp;Feng Shi ,&nbsp;Siyu Zhu ,&nbsp;Xue He ,&nbsp;Yiwen Ding ,&nbsp;Tianle Xu ,&nbsp;Dandan Qi ,&nbsp;Wei Chang ,&nbsp;Changlei Dai ,&nbsp;Fuqiang Song","doi":"10.1016/j.rhisph.2024.100973","DOIUrl":"10.1016/j.rhisph.2024.100973","url":null,"abstract":"<div><div>As a forage crop belonging to the Amaranthaceae family (often considered as non-mycorrhizal plants), <em>Amaranthus hypochondriacus</em> can grow in sodic soil. It is worth exploring whether arbuscular mycorrhizal fungi (AMF) can successfully form a symbiotic relationship with <em>A. hypochondriacus</em> to alleviate the environmental stress it experiences and improve rhizosphere soil quality. This study aims to evaluate the potential for AMF to form a symbiotic relationship with salt-tolerant Amaranthaceae plants. By measuring the colonization rate, biomass, and rhizosphere soil chemical properties of the plants, we assessed the response of <em>A. hypochondriacus</em> to AMF under different concentrations of saline-alkali stress. The results showed that <em>Rhizophagus intraradices</em> could form a good symbiotic relationship with <em>A. hypochondriacus</em> and reduce the rhizosphere soil pH and increase the effective nutrient content.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142446953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced biochemical properties of soybean root nodule asparaginase through plant molecular farming compared to bacterial enzyme for cancer treatment","authors":"Fahad Alharthi ,&nbsp;Hussam A. Althagafi ,&nbsp;Ibrahim Jafri ,&nbsp;Atif Abdulwahab A. Oyouni ,&nbsp;Mohammed M. Althaqafi ,&nbsp;Nawal E. Al-Hazmi ,&nbsp;Layla Yousif Abdullah Al Hijab ,&nbsp;Deyala M. Naguib","doi":"10.1016/j.rhisph.2024.100970","DOIUrl":"10.1016/j.rhisph.2024.100970","url":null,"abstract":"<div><div>Asparaginase is a therapeutic enzyme used as an anticancer agent and is typically produced through microbial fermentation using organisms such as <em>Escherichia coli</em> and <em>Erwinia chrysanthemi</em>. However, this method faces challenges, including potential enzyme contamination during production, allergic reactions to the enzyme, and stability issues requiring stringent control measures. An innovative solution is the application of plant molecular farming, utilizing Rhizobium root symbiosis for asparaginase production. The objective is to optimize nodule development for asparaginase yield, characterize the enzyme's properties, and evaluate its anticancer efficacy against microbial enzyme. In our study, we established soybean root cultures and inoculated them with <em>Bradyrhizobium japonicum</em> to form root nodules. We evaluated eukaryotic asparaginase production at different incubation times. We purified asparaginase from the root nodule cultures and compared its physicochemical properties and anticancer activity with microbial asparaginase. Results showed that asparaginase reached maximum activity in root nodule cultures 10 days after rhizobium inoculation in the culture media. The root nodule asparaginase exhibited a high content of alpha helices and beta sheets and a low random coil. It demonstrated higher stability and activity across different pH levels and temperatures than <em>Escherichia coli</em> asparaginase. Additionally, root nodule asparaginase displayed better catalytic parameters and stability over time than <em>E. coli</em> asparaginase. Thus, root nodule asparaginase is superior to <em>E. coli</em> asparaginase as an anticancer agent. This ensures the root nodule asparaginase can effectively target cancer cells, enhancing the overall therapeutic outcome. This provides a renewable, cost-effective, and environmentally friendly alternative to traditional enzyme production methods.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142528727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbiome analysis of the lithophytic resurrection plant Ramonda heldreichii, reveals root driven tight-rhizosphere vs elevation specific loose-rhizosphere communities","authors":"Kusum Dhakar ,&nbsp;Loukia M. Kellari ,&nbsp;Panagiotis A. Karas ,&nbsp;Athanasios Theodorakopoulos ,&nbsp;Michael N. Styllas ,&nbsp;Evangelia S. Papadopoulou ,&nbsp;Dimitrios G. Karpouzas ,&nbsp;Kalliope K. Papadopoulou ,&nbsp;Sotirios Vasileiadis","doi":"10.1016/j.rhisph.2024.100969","DOIUrl":"10.1016/j.rhisph.2024.100969","url":null,"abstract":"<div><div>We investigated the root microbiome of a relict resurrection (desiccation/frost-tolerant/resistant) plant, endemic to Mount Olympus (Litochoro, Greece), <em>Ramonda heldreichii</em> (Boiss.) C.B.Clarke, at various altitudes (400 m–1200 m asl), through amplicon sequencing. Microbial communities (prokaryotes, fungi, protists) revealed the significant impact of roots on the tight rhizosphere (TR) that were less diverse and less altitude-impacted compared with the loose rhizosphere (LR). Prokaryotic α-diversity was highly affected by root, whereas that of fungi was comparatively more sensitive to altitude. The TR-associated taxonomic groups, included well equipped taxa for tolerating biotic and abiotic stresses (drought/metal tolerance, microcystin degradation, psychrotolerance, chitin degradation) with Cercozoa dominating protists, while the LR-associated taxa mainly included microorganisms with chemolithoautotrophic potential. Relative abundances of the N-cycling and greenhouse gas associated <em>Nitrosopheraceae</em>, were increased with altitude. Collectively, the study of <em>R. heldreichii</em> demonstrated a plant-driven TR with bioprospecting potential, and an elevation-shaped and climate-linked LR, providing novel insights about mountain microbiology.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142432926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Essential oil phytochemistry and antifungal activity of lemongrass inoculated with arbuscular mycorrhizal fungi under different phosphorous levels","authors":"Caroline Lermen ,&nbsp;Rayane Monique Sete da Cruz ,&nbsp;Carlos Henrique de Souza Gonçalves ,&nbsp;Mariana Moraes Pinc ,&nbsp;Joice Karina Otênio ,&nbsp;Camila da Silva ,&nbsp;Odair Alberton","doi":"10.1016/j.rhisph.2024.100968","DOIUrl":"10.1016/j.rhisph.2024.100968","url":null,"abstract":"<div><div>This study aimed to investigate the effect of inoculating <em>Cymbopogon citratus</em> (lemongrass) with arbuscular mycorrhizal fungi (AMF) <em>Rhizophagus clarus</em> and <em>Claroideoglomus etunicatum</em> under low and high phosphorus (P) levels on essential oil (EO) content, composition, and minimum inhibitory concentration (MIC) for four isolates of phytopathogenic fungi of the genus <em>Fusarium</em>. The EO content was obtained by the hydrodistillation process, followed by the evaluation of chemical constituents using gas chromatography coupled to mass spectrometry and MIC by microdilution in broth. The EO content increased 2.5-fold due to high P and inoculation with <em>C. etunicatum</em> compared with uninoculated under low P. Twenty-two compounds were identified in EO. The major components were citronellol, geraniol, citral, neral and geranial. The EO of lemongrass showed a MIC in the four phytopathogenic fungal isolates. Therefore, the secondary metabolites were influenced by P levels and AMF inoculation, increasing EO's content (until 2.5-fold), chemical composition (citral, neral and geranial) and MIC.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142528598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustainable farming practices enhance bacterial diversity and nutrient levels in sorghum rhizosphere soil","authors":"S.P. Lalid Kumar ,&nbsp;M.R. Latha ,&nbsp;P. Janaki ,&nbsp;E. Parameswari ,&nbsp;T. Kalaiselvi ,&nbsp;D. Senthamilselvi ,&nbsp;R. Krishnan","doi":"10.1016/j.rhisph.2024.100967","DOIUrl":"10.1016/j.rhisph.2024.100967","url":null,"abstract":"<div><div>This research employs metagenomic analysis to explore bacterial diversity in sorghum rhizosphere soil in response to various sustainable farming practices and their impact on soil fertility. The field experiment with cotton-sorghum cropping system was conducted since 2020 and from 3rd cycle sorghum experiment, the soils were collected and subjected to metagenomics and nutrients availability analysis. We evaluated the impact of different farming approaches: Natural Farming (NF), Organic Farming (OF), integrated crop management (ICM), along with a control (No input farming). The results underscores the prevalence of Proteobacteria phylum across all farming practices, while Actinobacteria, Bacteroidetes and Firmicutes showed increased presence due to altered soil management and nutrient availability changes. Genus-level analysis revealed shifts in dominant genera, including <em>Acinetobacter</em>, <em>Sphingomonas, Bacillus</em>, and <em>Candidatus nitrososphaera</em>, across different farming practices. Post-harvest soil analysis indicated varied nutrient levels, with ICM showing enhanced nitrogen, phosphorus, and potassium availability. ICM, utilizing a balanced mix of organic and inorganic fertilizers, promoted sorghum growth, yielding higher plant height and total dry matter compared to OF and NF, which performed similarly. The study emphasizes the efficacy of a balanced approach like ICM for improving crop production and soil nutrient availability. However, all diversified farming practices exhibited high soil biological diversity compared to the control, highlighting the necessity for continuous long-term soil monitoring across different farming practices to stabilize soil microbiomes, ensure nutrient availability, and support sustainable crop production.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142424012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Short-term continuous cropping leads to a decline in rhizosphere soil fertility by modulating the perilla root exudates","authors":"Yaqi Liu ,&nbsp;Mengmeng Xie ,&nbsp;Tongtong Xue ,&nbsp;Xin Sui ,&nbsp;Hui Sun ,&nbsp;Chongwei Li ,&nbsp;Fuqiang Song","doi":"10.1016/j.rhisph.2024.100966","DOIUrl":"10.1016/j.rhisph.2024.100966","url":null,"abstract":"<div><div>Continuous cropping obstacles are a prevalent issue in agricultural production worldwide. However, the key factors leading to these obstacles differ among various crops. This study utilized high-throughput sequencing and metabolomics to assess the community structure of microbes and the types and abundance of metabolites in rhizosphere soil after different continuous cropping years of <em>Perilla frutescens</em> L. Britt. (perilla). The results indicate that differential metabolites in soils of varying continuous cropping years are primarily associated with pathways for cellular lipid formation, oxidative stress responses, and energy metabolism. These findings suggest that the changes in soil composition over time are linked to specific biological processes related to lipid formation, oxidative stress, and energy metabolism. The relative abundance of beneficial bacteria in the soil, such as <em>Sphingomonas</em>, <em>Gemmatimonas</em>, and <em>Blastococcus</em>, decreased significantly (<em>p</em> &lt; 0.05), while harmful fungi, such as <em>Neocosmospora</em> and <em>Didymella</em>, increased in response to the increase in continuous cropping years (<em>p</em> &lt; 0.05). The symbiotic network between bacteria and fungi has become less dense and stable after continuous cropping. Enzymatic activities in rhizosphere soil decreased significantly (<em>p</em> &lt; 0.05). Soil organic matter, total nitrogen, total phosphorus, total potassium, available phosphorus, and available potassium declined significantly (<em>p</em> &lt; 0.05). Pearson correlation analysis identified 10 differential secondary metabolites that were closely related to the relative abundance of key microorganisms. Among them, cinnamic acid and flavonoid compounds are key factors leading to changes in microbial diversity, and they can regulate the microorganisms-soil- plants interactions. In summary, this study provides a novel scientific explanation for how continuous cropping can reduce soil fertility and a theoretical basis for subsequent cultivation strategies of perilla.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142424011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}