Rhizosphere最新文献

{"title":"Transmission of bacterial community from seeds and rhizosphere to progeny in lima bean (Phaseolus lunatus)","authors":"Sandra Mara Barbosa Rocha ,&nbsp;Janderson Moura da Silva ,&nbsp;Romario Martins Costa ,&nbsp;Mayanna Karlla Lima Costa ,&nbsp;Regina Lucia Ferreira Gomes ,&nbsp;Ângela Celis de Almeida Lopes ,&nbsp;Ana Roberta Lima de Miranda ,&nbsp;Ademir Sérgio Ferreira Araujo","doi":"10.1016/j.rhisph.2025.101083","DOIUrl":"10.1016/j.rhisph.2025.101083","url":null,"abstract":"<div><div>The transmission of bacterial community from seeds and rhizosphere to progeny may have significant implications to next plant generations. Therefore, we evaluated the potential for transmission of bacterial taxa from seeds and rhizosphere to progeny and assessed the composition of the bacterial community in the soil, rhizosphere, and various plant compartments, including roots, leaves, and seeds. Seeds of lima bean (<em>Phaseolus lunatus</em>) were sown and during the flowering period, the soil, rhizosphere, roots, leaves, and seeds (progeny) were sampled. The V4 and V57 regions of 16S rRNA gene with region-specific primers were sequenced to soil/rhizosphere and plant, respectively. The composition of bacterial communities varied across soil and plant compartments at both the phylum and genus levels. Actinobacteria was abundant in soil and rhizosphere, while Proteobacteria was abundant in seeds and plant tissues. Genus-level differences included <em>Bacillus</em> in soil, rhizosphere, and roots, <em>Pseudomonas</em> in leaves, and <em>Acinetobacter</em> and <em>Pseudomonas</em> in seeds. Shared and exclusive taxa highlighted compartmental specificity in seeds and progeny. Thus, seeds and progeny exhibited 9 and 6 exclusive bacterial taxa, respectively, with <em>Acinetobacter</em>, <em>Pseudomonas</em>, and <em>Acidibacter</em> shared between them. This study identified distinct bacterial taxa across plant compartments and shows thar bacterial transmission to progeny highlights potential for next generations.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"34 ","pages":"Article 101083"},"PeriodicalIF":3.4,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143833613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Funneliformis mosseae exhibits greater improved effects on root hair development and phosphorus uptake in trifoliate orange than Claroideoglomus etunicatum","authors":"Chun-Yan Liu ,&nbsp;Xiao-Niu Guo ,&nbsp;Mashael Daghash Alqahtani ,&nbsp;Qiang-Sheng Wu","doi":"10.1016/j.rhisph.2025.101081","DOIUrl":"10.1016/j.rhisph.2025.101081","url":null,"abstract":"<div><div>Low-phosphorus (P) soils significantly limit agricultural productivity globally, particularly in regions where fertilizer application is restricted. Root hairs are crucial for the absorption of water and nutrients, while arbuscular mycorrhizal fungi (AMF) offer a promising approach to improve both root development and nutrient uptake. However, the molecular mechanisms by which specific AMF species affect root hair development in trifoliate orange (<em>Poncirus trifoliata</em>), a key citrus rootstock, are not well understood. In this study, the effects of <em>Funneliformis mosseae</em> (<em>F. mosseae</em>) and <em>Claroideoglomus etunicatum</em> (<em>C. etunicatum</em>) on root hair development, nutrient levels, and the expression of associated genes in trifoliate orange seedlings were studied. Both <em>F. mosseae</em> and <em>C</em>. <em>etunicatum</em> established symbiotic structures in the roots, but <em>F. mosseae</em> elicited a more pronounced response, as evidenced by enhanced plant growth, root development, root hair density, and length under <em>F. mosseae</em> versus <em>C. etunicatum</em>. Additionally, the two fungi increased phosphorus content and indole-3-acetic acid (IAA) levels, enhanced acid phosphatase activity, and reduced IAA oxidase activity, although <em>C. etunicatum</em> had a lesser effect on root IAA oxidase activity. The two fungal treatments upregulated the expression of <em>PtPAP1</em>, <em>PtaPT1</em>, and <em>PtaPT5</em>, while its down-regulated the expression of <em>PtPAP15</em>, <em>PtEXPA2</em>, and <em>PtEXPA4</em>. Correlation analysis revealed that <em>PtPAP1</em>, <em>PtaPT1</em>, and <em>PtaPT5</em> positively influenced root hair formation, while <em>PtPAP15</em>, <em>PtLAX5</em>, <em>PtABCB15</em>, and <em>PtPIN1</em> had negative effects on root hair formation. Overall, under low-phosphorus conditions, <em>F. mosseae</em> outperformed <em>C. etunicatum</em> in promoting root hair development and phosphorus uptake in trifoliate orange, driven by enhanced colonization, elevated IAA levels, and upregulated phosphate transporter genes. These findings highlight <em>F. mosseae</em>'s superior potential for optimizing root architecture and nutrient efficiency in citrus rootstocks.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"34 ","pages":"Article 101081"},"PeriodicalIF":3.4,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143845012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stem rot affects the structure of rhizosphere microbiome in berseem clover (Trifolium alexandrinum)","authors":"Salma Mukhtar ,&nbsp;Zain Ahmad ,&nbsp;Noor Khan ,&nbsp;Tianyi Xu ,&nbsp;Dalaq Aiysha","doi":"10.1016/j.rhisph.2025.101073","DOIUrl":"10.1016/j.rhisph.2025.101073","url":null,"abstract":"<div><div>Rhizosphere microbiome plays an essential role in maintaining plant health and productivity.</div><div>Fungal and bacterial diseases may affect the rhizosphere-associated microbial communities and overall structure of plant microbiome. Here, we studied the effect of stem rot of berseem clover on the bacterial and fungal communities associated with the rhizosphere. We analyzed the rhizosphere-associated bacterial and fungal microbiome from healthy and infected berseem clover collected from three sampling sites by using 16S rRNA and ITS based Illumina sequencing metabarcoding approach. Microbiome analysis showed that healthy plants had higher bacterial and fungal diversity as compared to stem rot infected plants. At the genus level, bacterial genera <em>Rhizobium</em> and <em>Comamonas</em> were more abundant in healthy plants while <em>Pantoea</em> was more abundant in infected plants and fungal genera <em>Sclerotinia</em>, <em>Fusarium</em> and <em>Podospora</em> were more abundant in infested plants while <em>Rhizoglomus</em> and <em>Cladosporium</em> were distinctively abundant in healthy Berseem. Functional characterization of bacterial and fungal microbiomes revealed that bacterial communities from infected plants showed more abundance of bacteria with functions replication and repair, enzyme families and biosynthesis of other secondary metabolites as compared to healthy plant microbiome and decreased in fungal groups including arbuscular mycorrhiza and soil saprotrophs and an increase in plant saprotrophs and fungal parasite-plant pathogens. This study provides comprehensive information about the structure and composition of bacterial and fungal communities associated with the berseem clover rhizosphere that could be utilized for future research on the control of stem rot of berseem clover.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"34 ","pages":"Article 101073"},"PeriodicalIF":3.4,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenological shifts drive rhizosphere microbial community dynamics in Subtropical woody bamboo (Chimonobambusa utilis (Keng) P. C. Keng): pH and total phosphorus as main drivers","authors":"Zhiming Zhang ,&nbsp;Yurong Cao ,&nbsp;Maosheng Sun ,&nbsp;Lianchun Wang ,&nbsp;Hanqi Yang","doi":"10.1016/j.rhisph.2025.101072","DOIUrl":"10.1016/j.rhisph.2025.101072","url":null,"abstract":"<div><div>As a high-value clonal plant with rapid reproduction and growth characteristics, the relationship between the phenology of bamboo and the changes in rhizosphere microbial communities is still in its infancy. Here, we explored the change trends of composition and structure of rhizospheric microbial communities at key phenological stages in a medium-mountain woody bamboo <em>Chimonobambusa utilis</em> (Keng) P. C. Keng stands. This study employed Illumina high-throughput sequencing of 16S and ITS fragments to examine the dynamic changes in rhizosphere microbes from the dormancy stage to the shooting stages of <em>Ch</em>. <em>utilis</em>, as well as their relationship with soil physicochemical properties. <em>Ch. utilis</em> rhizosphere microbes had greater numbers of operational taxonomic units (OTUs), higher α-diversity, more complex and stable microbial community structure during the shooting stage, with more keystone species including <em>Arthrobacter</em>, <em>Leohumicola</em>, <em>Sarocladium</em>, etc. Moreover, rhizosphere microbes at the dormancy and shooting stages exhibited different preferences for soil physicochemical properties, and particularly soil total phosphorus (TP) demonstrated a more significantly positive correlation with rhizosphere microbes at the shooting stage. This study reveals the dynamic changes of rhizosphere microorganisms in different growth stages of subtropical bamboo, highlighting the significant impact of soil pH and TP on the composition of rhizosphere microorganisms during the bamboo shooting stage. This work provides new insights into the relationship among bamboo, soil and soil microorganisms, as well as utilization of rhizosphere rhizobial communities for enhancing sustainable productivity and ecological management of bamboo forests.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"34 ","pages":"Article 101072"},"PeriodicalIF":3.4,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143848241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impacts of soil environment on the growth and quality of Cynanchum auriculatum mediated by rhizosphere microorganisms","authors":"Junjie Tang ,&nbsp;Xiaomeng Fei ,&nbsp;Yinzhi Wu ,&nbsp;Wenqing Wu ,&nbsp;Min Tang ,&nbsp;Wenda Xue ,&nbsp;Xudong Qian ,&nbsp;Daoguo Zhang ,&nbsp;Wei Gu","doi":"10.1016/j.rhisph.2025.101078","DOIUrl":"10.1016/j.rhisph.2025.101078","url":null,"abstract":"<div><div>The growth and quality of <em>Cynanchum auriculatum</em> is profoundly influenced by the soil environment, where multi-year cultivation leading to a reduction in growth potential and an increased susceptibility to diseases. Despite these impacts, there is a lack of comprehensive reports on how the soil environment specifically affects the growth and quality of <em>C. auriculatum</em>. In this study, we collected rhizosphere soil and root samples of <em>C. auriculatum</em>. We determined the soil composition, carbon metabolic capacity, and potential functions using metagenomic techniques, and analyzed the chemical constituents in the roots. We found that microbial metabolic capacity for phenolic acid carbon sources declined significantly in <em>C. auriculatum</em> rhizosphere soils from the third-year compared to the second-year. As planting years increased, the diversity of the rhizosphere soil bacterial community decreased. <em>Bradyrhizobium</em> and <em>Lysobacter</em> were identified as the dominant bacterial genera in the rhizosphere soil of <em>C. auriculatum</em>. The soil environmental factors influencing changes in bacterial and fungal communities in rhizosphere soil were identified as total nitrogen, available nitrogen, available phosphorus, available potassium, alkaline phosphatase, and catalase. These factors influenced the function of metabolic genes in rhizosphere microorganisms, leading to a decrease in metabolic activity and a disruption in the dynamic balance of microorganisms. Our research can provide a theoretical basis for enhancing the rhizosphere soil environment, optimizing management practices, and increasing the productivity and quality of <em>C. auriculatum</em>.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"34 ","pages":"Article 101078"},"PeriodicalIF":3.4,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143824188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temperature and host plant ecotype drive nitrogen fixation, but not nodule rhizobia community composition, in hairy vetch","authors":"Rebecca Fudge ,&nbsp;Paula Gardner ,&nbsp;R. Ford Denison ,&nbsp;Liana T Burghardt ,&nbsp;Julie Grossman","doi":"10.1016/j.rhisph.2025.101077","DOIUrl":"10.1016/j.rhisph.2025.101077","url":null,"abstract":"<div><div>Hairy vetch (<em>Vicia villosa</em> Roth) is a commonly grown cover crop throughout the U.S., which can contribute nitrogen for subsequent cash crops through biological nitrogen fixation (BNF) in association with <em>Rhizobium leguminosarum</em> biovar <em>viciae</em> (Rlv) bacteria. Hairy vetch is one of the few cover crops sufficiently cold-tolerant to over-winter in the Upper Midwestern U.S. However, nitrogen contributions by hairy vetch vary across locations, potentially due to cold impacts on the legume/rhizobia symbiosis. The traditional route to improve BNF in legumes involves selecting superior rhizobia strains to create more effective inoculants to apply at planting, but inoculants often fail to compete and survive in agricultural soils. Instead, this study tested the effects of temperature and host plant ecotype on hairy vetch BNF and Rlv community composition in nodules, with the goal of potentially identifying vetch ecotypes able to select beneficial Rlv strains from the soil community. Four hairy vetch ecotypes trapped Rlv from three Minnesota soils, at warm or cold temperatures. Vetch ecotype was a key driver of BNF and nodule formation under warm and cold conditions. However, temperature and plant ecotype did not drive Rlv community composition in nodules, and Rlv community composition did not affect plant productivity. Taken together, these results suggest that the best strategy to improve BNF at low temperatures in hairy vetch likely depends on breeding for improved biomass accumulation and nitrogen fixation in host plants, rather than focusing on host plant selection of beneficial rhizobia.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"34 ","pages":"Article 101077"},"PeriodicalIF":3.4,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143820486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arbuscular mycorrhizal colonization promotes plant growth and regulates biochemical and molecular defense responses against Pythium myriotylum and Meloidogyne incognita in ginger (Zingiber officinale Rosc.)","authors":"C. Sarathambal ,&nbsp;B. Manimaran ,&nbsp;M Faisal Peeran,&nbsp;V. Srinivasan,&nbsp;R. Praveena,&nbsp;P. Gayathri,&nbsp;Fathima Dilkush,&nbsp;Anitta Abraham","doi":"10.1016/j.rhisph.2025.101071","DOIUrl":"10.1016/j.rhisph.2025.101071","url":null,"abstract":"<div><div>This study investigated the effects of arbuscular mycorrhizal fungi (AMF) inoculation on growth, nutrient uptake, and defense mechanisms against <em>Pythium myriotylum</em> and <em>Meloidogyne incognita</em> in ginger (<em>Zingiber officinale</em> Rosc.). Structural colonization of AMF was confirmed in inoculated ginger roots, with colonization rates of 90 % and 80 % at two and six months, respectively. AMF significantly enhanced plant height and number of tillers compared to non-inoculated controls. Nutrient uptake was notably higher in AMF-inoculated plants, with increased levels of nitrogen (27.81 g wt plant⁻¹), phosphorus (3.89 g wt plant⁻¹), potassium, calcium, magnesium, and micronutrients. AMF inoculated plants demonstrated 50 % reduction in disease incidence when challenged with <em>P. myriotylum</em>. Similarly, AMF preinoculated ginger plants showed no <em>Meloidogyne incognita</em> (J2s) penetration in the roots, unlike plants treated only with <em>M. incognita</em> (J2s)<em>.</em> Additionally, protray experiment was conducted to study the possible correlation between the disease resistance traits of AMF using biochemical and molecular methods. In this study, the enhanced activity of antioxidant enzymes, such as peroxidase, catalase, and β-1,3-glucanase, was observed in AMF-primed plants under pathogen inoculation, particularly with combined infections of <em>P. myriotylum</em> and <em>M. incognita</em>, indicating improved systemic resistance. At the molecular level, AMF priming altered the expression of key defense-related genes. In AMF-treated plants, the <em>NPR1</em> gene, which regulates systemic acquired resistance, was notably upregulated in roots with <em>P. myriotylum</em>, while <em>TGA</em> and <em>AP2</em>, involved in salicylic acid signaling and stress response, exhibited strong expression in the presence of AMF and <em>M. incognita</em>. In leaves, <em>LOX2</em>, a key enzyme in jasmonic acid biosynthesis, showed the highest expression with <em>M. incognita</em>, whereas <em>AOC</em>, essential for jasmonate signaling, steadily increased in AMF-primed plants with <em>P. myriotylum</em>. These findings indicate that AMF colonization supports nutrient uptake, plant growth, and systemic defense, thereby enhancing resistance to pathogen infections in ginger.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"34 ","pages":"Article 101071"},"PeriodicalIF":3.4,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143833612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inoculation of wild type or EPS mutant Bacillus subtilis does not alter rhizocompetence, soil EPS, and tomato growth in the absence of stress","authors":"Antje Müller ,&nbsp;Jeroen H.T. Zethof ,&nbsp;Camilla Pirani ,&nbsp;Cordula Vogel ,&nbsp;Lorrie Maccario ,&nbsp;Søren J. Sørensen ,&nbsp;Kornelia Smalla ,&nbsp;Karsten Kalbitz ,&nbsp;Doreen Babin","doi":"10.1016/j.rhisph.2025.101076","DOIUrl":"10.1016/j.rhisph.2025.101076","url":null,"abstract":"<div><div>Biofilm formation on plant roots is traditionally assumed an important trait for plant growth promoting rhizobacteria, but its importance in the interplay between soil, plant roots and the rhizosphere bacterial and archaeal community is not well understood. In a greenhouse experiment, tomato plants grown in a sandy soil were inoculated with <em>Bacillus subtilis</em> NCIB 3610 wild type or a <em>ΔepsA-O</em> mutant strain with defective biofilm formation. Strain-dependent effects on rhizocompetence, bacterial/archaeal community composition, soil EPS contents and plant growth were hypothesized. High-throughput sequencing of 16S rRNA genes amplified from community DNA showed that inoculation-dependent shifts of the bacterial and archaeal community compositions occurred mainly in the rhizoplane and rhizosphere between one or eight days after inoculation. Contrary to our hypothesis, the mutant had a similar rhizocompetence as the wild type according to selective plating. The EPS-saccharide content in the soil and rhizosphere did not differ among inoculation treatments. Plants inoculated with the mutant had larger roots compared to the wild type and control treatments – likely due to inoculation-dependent bacterial and archaeal community shifts. Thus, this study shows that the inoculants′ ability to produce biofilms was not linked with rhizocompetence, EPS contents in soil and plant growth. It sheds light on new aspects regarding the relevance of biofilm formation for rhizocompetence and plant growth promotion under optimal plant growth conditions.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"34 ","pages":"Article 101076"},"PeriodicalIF":3.4,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143891144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acidic compost tea reduces fungal diversity but improves community structure and P availability in calcareous cotton fields","authors":"Liyang Cheng ,&nbsp;Tong Luo ,&nbsp;Tao Min ,&nbsp;Junhua Li","doi":"10.1016/j.rhisph.2025.101066","DOIUrl":"10.1016/j.rhisph.2025.101066","url":null,"abstract":"<div><div>Acidic compost tea (CT) can provide nutrients for cotton in calcareous (alkaline) soil and improve soil properties; however, the responses of fungi to rhizosphere nutrient conditions and physicochemical properties remain unclear. In this study, the responses of phosphorus (P) morphology and fungal community structure and function to CT in cotton rhizosphere soil were examined. The results indicated that the contents of water P, NaHCO₃-P, and NaOH-P in rhizosphere soil as well as the absorption and accumulation of nutrients by cotton were significantly increased following CT application. The diversity of rhizosphere fungi decreased; however, the species formed a more complex and stable community. Moreover, the relative abundance of oligotrophic species, such as <em>Basidiomycota</em>, decreased. Random forest distribution and Mantel tests revealed that the main driving factors in the compositional change of the fungal species were soil pH and the increasing proportion of labile P (water P and NaHCO₃-P) and moderately labile P (NaOH-P and HCl-P), which directly affected the abundance of species with varying nutrient modes. A structural equation model revealed that the primary factor for dry matter accumulation and yield increase in cotton was increased P availability. This study revealed the changes in P morphology and fungal community in rhizosphere soil in response to CT and factors contributing to these changes as well as evaluated the relationship between efficient utilization of P in calcareous soil and rhizosphere fungal microecology.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"34 ","pages":"Article 101066"},"PeriodicalIF":3.4,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143820547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancement of Fe tolerance in Polygonum thunbergii naturally growing in mine pond at the reproduction stage","authors":"Mitsuki Yachi ,&nbsp;Keiko Yamaji ,&nbsp;Kohei Doyama ,&nbsp;Toshikatsu Haruma ,&nbsp;Xingyan Lu","doi":"10.1016/j.rhisph.2025.101068","DOIUrl":"10.1016/j.rhisph.2025.101068","url":null,"abstract":"<div><div><em>Polygonum thunbergii</em> Sieb. et Zucc., an annual herb, has been known to grow naturally at mine sites. This study examined the Fe tolerance mechanisms in <em>P. thunbergii</em> growing in a mine pond, considering carbon allocation to potential Fe-tolerance factors. Fe concentrations in <em>P. thunbergii</em> collected from June to October 2021, were high especially in live and dead adventitious roots. In the live adventitious roots, Fe detoxicants (phenolic compounds; gallic acid, procyanidin B2, procyanidin A2, and condensed tannins) and Fe localization in the epidermal cell walls could influence Fe tolerance. Fe accumulation in the dead adventitious roots could be related to Fe tolerance because metal accumulation in dead plant tissues is important to decrease Fe toxicity as exclusion site. In addition, the proportion of carbon used for phenolic compounds significantly increased in live adventitious roots at the flowering time in September. Significantly less carbon for Fe exclusion by dead adventitious roots was also detected at the flowering time. Our results suggest that in order to establish the next generation<em>, P. thunbergii</em> could enhance Fe tolerance at the reproduction stage.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"34 ","pages":"Article 101068"},"PeriodicalIF":3.4,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143820487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}