Rhizosphere最新文献

{"title":"The root extracellular trap: A checkpoint controlling root tip accessibility to microorganisms","authors":"Alexia Gaudry ,&nbsp;Magalie Bénard ,&nbsp;Agnès Attard ,&nbsp;Eric Nguema-Ona ,&nbsp;Azeddine Driouich ,&nbsp;Barbara Pawlak","doi":"10.1016/j.rhisph.2025.101016","DOIUrl":"10.1016/j.rhisph.2025.101016","url":null,"abstract":"<div><div>The root tip and root-Associated Cap-Derived Cells (AC-DCs) release various organic compounds into the rhizosphere, forming the Root Extracellular Trap (RET), a network involved in root-soil microorganism interactions and root protection. This study investigates the role of soybean (<em>Glycine max</em>) and pea (<em>Pisum sativum</em>) RETs in interactions with soil rhizobacteria (<em>Bacillus subtilis</em> and <em>Pseudomonas fluorescens</em>) and the zoospores of the oomycete <em>Phytophthora parasitica</em> by examining their impact on microbial behavior. To this end, confrontation tests were performed, followed by imaging analyses of videos, in which the behavior of microorganisms (<em>i.e.,</em> swimming speeds and trajectories) was characterized and quantified. The results show that the RET alters microbial access to the root tip and modifies significantly speeds and trajectories. Inside the RET, the speeds of <em>B. subtilis</em> and <em>P. parasitica</em> zoospores decreased three and nine times respectively. Outside the RET, the speed of the bacterium remains unchanged, while that of the zoospores decreases twice. These findings highlight the influence of the RET on microbial movement and its importance in plant-microorganism interactions in the rhizosphere.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"33 ","pages":"Article 101016"},"PeriodicalIF":3.4,"publicationDate":"2025-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143137770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diverging role of phytohormones and soil nutrients between two broad and narrow-distribution orchids of Satyrium species","authors":"Brihaspati Poudel,&nbsp;Taiqiang Li,&nbsp;Rengasamy Anbazhakan,&nbsp;Jiangyun Gao","doi":"10.1016/j.rhisph.2024.101013","DOIUrl":"10.1016/j.rhisph.2024.101013","url":null,"abstract":"<div><div>The community assembly of root-associated endophytic fungi not depend on the distribution pattern of host orchids at the local level. In each case, the host plant uses soil nutrients and host compounds to attract endophytic fungi; yet, it is still necessary to investigate how these factors affect the distribution of sample orchids and their associates. Therefore, we assessed the diverging role of phytohormones and soil nutrients in their host distribution. The root-associated fungi were investigated using the amplicon sequencing method and evaluated the host chemical profiles/soil nutrients associated with the roots of sample species using Gas Chromatography-Mass Spectrometry and High Performance-Liquid Chromatography. Finally, we used the correlation analysis method to examine their relationship with endophytic fungi. Using soil samples that were taken, we assessed nine soil nutrients along with soil pH. Likewise, 22 phytohormones were found in the roots of sample orchids. Where, both sample orchids (<em>Satyrium nepalense</em> D. Don. and <em>Satyrium yunnanense</em> Rolfe.) were dominated by distinct endophytic fungal families (Unclassified_k_fungi and Hygrophoraceae) independently, and they showed either positive or negative correlation with phytohormones and soil nutrients based on the distribution pattern of host orchids. The narrowly-distributed orchid was rich by soil nutrients and endophytic fungi but the broadly-distributed orchid was rich by phytohormones and poor in fungi composition in local level So, the diversity and community composition of endophytic fungi was not related to phytohormones but positively related with soil nutrients in local level. Our limited data cannot provide strong evidence but this finding may open the door for further study over the distribution of orchids and their symbionts. Finally, our results help in ecological resilience and potential biotechnological application or conservation of orchids and their symbionts.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"33 ","pages":"Article 101013"},"PeriodicalIF":3.4,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143137771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Host selection shapes structure and network of microbial community of Epimedium plants along the soil–rhizosphere–plant continuum","authors":"Zhaoyu Zhang ,&nbsp;Ziying Huang ,&nbsp;Xiao Chen ,&nbsp;Guozhuang Zhang ,&nbsp;Mengzhi Li ,&nbsp;Anning Li ,&nbsp;Xiaolin Lin ,&nbsp;Yuxin Zhou ,&nbsp;Yu Zhang ,&nbsp;Shilin Chen ,&nbsp;Linlin Dong ,&nbsp;Guangfei Wei","doi":"10.1016/j.rhisph.2024.101010","DOIUrl":"10.1016/j.rhisph.2024.101010","url":null,"abstract":"<div><h3>Background and aims</h3><div>Microbiota inhabiting plants are essential for plant productivity and health. Microbes in various plant compartment niches perform distinct ecological functions. However, the microbial characteristics in different niches of <em>Epimedium</em> plants, one of the most promising tonic herbs in traditional Chinese medicine, remain largely unknown.</div></div><div><h3>Methods</h3><div>Here, microbial composition, diversity and co-occurrence network in the five ecological niches (bulk soil, rhizosphere soil, root, stem, and leaf) of three <em>Epimedium</em> plant cultivars were investigated using high-throughput sequencing.</div></div><div><h3>Results</h3><div>The microbial composition were predominately imprinted by compartment niches than by host cultivars. Bacterial diversity and network complexity incrementally decreased from the soils to the root to the leaf; however, fungal diversity and network complexity gradually decreased from the soils to the root to the stem but increased from the stem to the leaf. Cyanobacteria, Proteobacteria, <em>Arthrobacter</em>, <em>Sphingomonas</em>, and <em>Gemmatimonas</em> were bacterial keystone taxa, while Ascomycota, <em>Davidiella</em>, and <em>Hymenoscyphu</em>s were fungal keystone taxa. These taxa exhibited significant compartment-specific properties and performed distinct ecological functions.</div></div><div><h3>Conclusion</h3><div>The microbial communities are mostly originated from bulk soil, and subsequently filtered and enriched in various compartment niches through host selection. Compartment niches profoundly imprinted the plant-associated microbiota, which provided valuable insights into harnessing beneficial communities for <em>Epimedium</em> plants in sustainable agriculture and the improvement of herbal quality.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"33 ","pages":"Article 101010"},"PeriodicalIF":3.4,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143137737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Root halotropism in plants: tolerance or escape?","authors":"Abhishek Mazumder ,&nbsp;Vikram Singh Gaur ,&nbsp;Paresh Chandra Kole ,&nbsp;Tapan Kumar Mondal","doi":"10.1016/j.rhisph.2024.101002","DOIUrl":"10.1016/j.rhisph.2024.101002","url":null,"abstract":"<div><div>Natural world is composed of fascinating diversity of life-forms and numerous adaptation strategies in the changing environment especially in the plant kingdom. An adaptive plant root response in saline environment to circumnavigate a stressful atmosphere by a directional growth away from salinity is known as halotropism, which is accomplished by the redistribution of phytohormone auxin at the root tip by PIN-FORMED 2 (PIN2) auxin efflux carrier. In this article, we review the role of salt-induced phospholipase D activity, endocytosis of auxin efflux carrier in cell membrane, asymmetry of auxin distribution, ABCB transporters, protein phosphatase 2A, auxin metabolism, phytohormones, microtubule and cell wall glycoprotein in the regulation of root halotropism. As climate change and increased soil salinity are two most important causal factors behind reduction in yield and crop diversity threatening global agriculture, we have summarized key advances in root halotropism and emphasized the importance of gaining a comprehensive understanding on the mechanism of root halotropism in crop plants, which might enable in designing plants with modulated root architecture to enhance crop adaptability and yield in saline soil for ensuring food security across the globe.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"33 ","pages":"Article 101002"},"PeriodicalIF":3.4,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143137772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The appropriate concentration of L-aspartate nano-calcium promotes growth and potassium utilization in cigar varieties of tobacco (Nicotiana tabacum L.)","authors":"Xinying Chen ,&nbsp;Naheeda Begum ,&nbsp;Dezhi Kong ,&nbsp;Yanuo Gong ,&nbsp;Munib Ahmad ,&nbsp;Pingping Wang ,&nbsp;Suiling Ai ,&nbsp;Mushtaq Ahmad ,&nbsp;Lixin Zhang","doi":"10.1016/j.rhisph.2024.101009","DOIUrl":"10.1016/j.rhisph.2024.101009","url":null,"abstract":"<div><div>L-aspartate nano-calcium is a nanoscale amino acid chelated calcium that has been widely used as a human calcium nutrient enhancer. However, its role in the field of botany remains to be fully elucidated. This study is the first to investigate the use of L-aspartate nano-calcium as a calcium source for tobacco (<em>Nicotiana tabacum</em> L.). In this study, two tobacco plant varieties for cigars, a potassium-efficient and a potassium-inefficient variety, were cultivated using a conventional source of calcium (nitrate), alongside a range of concentration gradients of L-aspartate nano-calcium (0, 25, 50, 75, 100 and 150 mg L⁻¹). The impact of L-aspartate nano-calcium on the growth of cigar varieties with varying potassium efficiency types was examined in terms of morphology, physiology, and potassium absorption and utilization. Furthermore, an EW-TOPSIS coupling model was established. The results of the multi-objective decision-making and evaluation process, conducted using the EW-TOPSIS method, indicate that L-aspartate nano-calcium can serve as a novel calcium source for maintaining the growth of cigar varieties. Additionally, the findings suggest that a concentration of 150 mg L⁻¹ L-aspartate nano-calcium is the most beneficial for the potassium development and utilization and is applicable to both the potassium-efficient variety and the potassium-inefficient variety. The study provides a scientific basis for improving cigar varieties quality, enhancing potassium utilization efficiency and utilizing L-aspartate nano-calcium as a novel calcium supplement in botany.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"33 ","pages":"Article 101009"},"PeriodicalIF":3.4,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143137732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mucilage increases soil resistance to penetration after compaction","authors":"Asma Fathinejad Poshkoohi ,&nbsp;Mohammad Hossein Mohammadi ,&nbsp;Mohsen Zarebanadkouki ,&nbsp;Hassan Etesami","doi":"10.1016/j.rhisph.2024.101014","DOIUrl":"10.1016/j.rhisph.2024.101014","url":null,"abstract":"<div><div>Mucilage offers several beneficial functions for soils, yet its impact on soil mechanical behavior remains underexplored. This study investigated the effects of chia seed mucilage on the plasticity index (under normal conditions) and penetration resistance (under compaction) of sandy clay loam soils with differing soil organic carbon (SOC) levels from Akhtar Abad (SOC = 1.6%) and Najm Abad (SOC = 0.6%) in northern Iran. Four mucilage concentrations (0, 1 g kg⁻¹, 3 g kg⁻¹, 5 g kg⁻¹) and three compaction pressures (100 kPa, 300 kPa, 600 kPa) were used. We found that mucilage significantly increased the plasticity index, with a 5.8% increase at 1 g kg⁻¹ in the Najm Abad soil and 2.6%–3% increases at higher concentrations in the Akhtar Abad soil. At a concentration of 3 g kg⁻¹, soil penetration resistance in the Akhtar Abad soil increased by 0.9 MPa and 1.6 MPa at compaction pressures of 300 kPa and 600 kPa, respectively. In the Najm Abad soil, a concentration of 5 g kg⁻¹ led to increases of 0.7 MPa and 1.7 MPa at compaction pressures of 300 kPa and 600 kPa, respectively. No significant relationship was found between soil penetration resistance and soil plasticity index. The mucilage-induced increase in soil plasticity may hinder soil workability, especially when tillage occurs immediately after crop harvest. Mucilage can also increase soil resistance to root penetration in areas compacted by heavy machinery. To mitigate these risks, we recommend performing tillage and machinery operations in both agricultural and forest ecosystems during dry periods, when mucilage is less active, to minimize its negative impact on soil workability and compaction.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"33 ","pages":"Article 101014"},"PeriodicalIF":3.4,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143137733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel approach in soil zymography to assess enzyme activity in the rhizosphere","authors":"Iryna Loginova,&nbsp;Nataliya Bilyera","doi":"10.1016/j.rhisph.2024.101006","DOIUrl":"10.1016/j.rhisph.2024.101006","url":null,"abstract":"<div><div>Soil zymography is increasingly used to study belowground plant-soil interactions and microbial hotspots in the rhizosphere. Over the past decade, several approaches have been proposed to quantitatively estimate enzymatic activity in rhizosphere hotspots using root masks based on normal light (NL) root photos. However, these approaches are often time-consuming and typically applied to small regions of zymograms. Therefore, obtaining automated, but precise root masks for crops with various root system is necessary to improve and standardize soil zymography techniques in rhizosphere studies.</div><div>Here we introduced a newly developed approach that involves photographing roots under ultraviolet (UV) light after conducting zymography to create a root mask for six crops: maize, wheat, barley, rye, lupine, and faba bean grown on Loamy Luvisol. Our study revealed a significant improvement in the quality of root masks thresholded from UV light root photos compared to NL photos.</div><div>The β-glucosidase activity measured within the root mask thresholded from UV root photos enabled accurate estimation of the root-associated enzyme activity for all six crops. This offers substantial advantages over measuring enzyme activity across the entire zymogram, that often leads to imprecise interpretations of zymography results in the rhizosphere studies. Moreover, UV root photos taken after zymography allow to detect metabolically active and growing roots. Additionally, high contrast of UV root photos and clear delineation of roots on the soil surface for all studied crops enables using these photos for precise alignment of soil images of different origin in the co-localization analysis.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"33 ","pages":"Article 101006"},"PeriodicalIF":3.4,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143137734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cover crops as a strategy for root rot management in common beans: Effects on crop performance and root microbial dynamics in a Cerrado soil","authors":"Pablo Schulman ,&nbsp;Luiz Cesar Lopes Filho ,&nbsp;Juliana Pires Basílio ,&nbsp;Elder Tadeu Barbosa ,&nbsp;Nédio Tormen ,&nbsp;Rafael de Souza Nunes ,&nbsp;Nilvo Altmann ,&nbsp;Jadson Belém de Moura ,&nbsp;Murillo Lobo Junior","doi":"10.1016/j.rhisph.2024.101015","DOIUrl":"10.1016/j.rhisph.2024.101015","url":null,"abstract":"<div><div>No-till farming is a key restoration technique that helps preserve soil health and productivity without expanding cultivated areas. Here, we investigate the influence of different cover crops on the performance and root microbial diversity of common beans in a Brazilian Cerrado soil. The study was conducted for five years on a commercial farm, with six treatments and three replicates. Treatments were evaluated yearly for yield and dry root rot severity (<em>Fusarium</em> spp.). Rhizospheric soil samples were analyzed for microbiome diversity using metataxonomics in the last year of the experiment. Oat and sunn hemp consistently performed better for severity, while a mix of cover crops, oat and palisade grass resulted in higher yields than fallow. Diversity metrics did not vary between treatments for both bacterial and fungal communities. <em>Streptomyces</em> was prominent in oat and sunn hemp, while <em>Fusarium</em> dominated the fungal community across treatments with a relative abundance of &gt;35%. Characteristics prediction highlighted significant bacterial functions and fungal traits, with palisade grass showing distinct methanol oxidation and oat exhibiting diverse fungal endophytes, saprotrophs, and organic matter decomposers that may compete against <em>Fusarium</em> spp. Network analysis revealed variable co-occurrence patterns, with <em>Fusarium</em> spp. exhibiting different associations depending on the treatment. The network analysis also highlighted competing fungal genera that may guide biocontrol strategies. Mix, oat, and pearl millet were the cover crops favoring the highest number of genera negatively correlated with <em>Fusarium</em> spp. in the common bean rhizosphere. Future studies should explore the long-term effects and temporal dynamics of the common bean root microbiome.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"33 ","pages":"Article 101015"},"PeriodicalIF":3.4,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143137768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochar improves root growth and nitrogen metabolism in tobacco through physiological pathways modulation","authors":"Jie Yang ,&nbsp;Xiaoyan Dai ,&nbsp;Yuyin Zhang ,&nbsp;Qingli Xiao ,&nbsp;Chao Yang ,&nbsp;Pingwei Qin ,&nbsp;Chaopeng Song","doi":"10.1016/j.rhisph.2024.101011","DOIUrl":"10.1016/j.rhisph.2024.101011","url":null,"abstract":"<div><div>Biochar has been demonstrated to influence crop root development and nitrogen metabolism; however, its effects vary notably among different crop species. This study investigated the regulatory effects of biochar on the rhizosphere soil environment, root morphology and physiology, nitrogen metabolism-related enzymes and genes, and leaf metabolism in tobacco under field conditions. The results showed that biochar significantly enhanced tobacco root development and nitrogen metabolism efficiency. Specifically, biochar improved root vitality by 58.23%, and the activities of peroxidase and superoxide dismutase increased by 30.17% and 26.38%, respectively. Concurrently, biochar improved nitrogen metabolism efficiency by increasing soil nitrate nitrogen and ammonium nitrogen contents by 38.99% and 99.94%, respectively. Moreover, biochar treatment enhanced the activities of nitrate reductase and glutamine synthetase in roots by 28.14% and 41.87%, respectively. Additionally, biochar influenced root hormone levels, which positively affected root growth and played a critical role in regulating nitrogen metabolism. This study demonstrated that biochar modulates root development and nitrogen metabolism efficiency in tobacco through physiological changes. These findings offer valuable insights into the potential of biochar as a sustainable soil amendment to enhance crop productivity and reduce dependence on chemical fertilizers.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"33 ","pages":"Article 101011"},"PeriodicalIF":3.4,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143137675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated management of root-knot nematodes on tree tomato","authors":"Stanlous Juma Waswa ,&nbsp;Waceke J. Wanjohi ,&nbsp;Maina Mwangi","doi":"10.1016/j.rhisph.2024.101012","DOIUrl":"10.1016/j.rhisph.2024.101012","url":null,"abstract":"<div><div>Tree tomato is an economically and nutritionally important horticultural crop in Kenya and globally. However, this crop is attacked by root-knot nematodes (RKNs) resulting into yield losses. The study's objective was to establish two-season experiments to determine the effectiveness of combining <em>Colletotrichum nigrum</em> or commercial <em>Trichoderma asperellum</em> with <em>Lantana camara</em> L. leaf extract and Velum® (active ingredient = fluopyram) against RKNs on tree tomatoes under greenhouse and field conditions. Tree tomato roots were collected for isolation of <em>C</em>. <em>nigrum</em>. <em>Lantana</em> leaves were collected, dried, ground into powder and water extract obtained from 200g w/v. Pots containing seedlings were treated with 2000 infective juveniles of RKN in the greenhouse. The treatments were applied at transplanting. Field experiments were established on RKN-naturally infected plots. Greenhouse and field treatments were arranged in completely randomized and randomized complete block designs, respectively, with four replications. To asses RKN suppression, nematodes were extracted from 200 cm³ of composite rhizosphere soils or 5g of roots per treatment using modified Baermann extraction tray method and counted under the microscope. Plant height was measured in centimeters from the soil line to the newest apical shoot and compared. Shoots were separated from roots at the base, labelled, oven-dried and weight recorded in grams while nematode reproduction factor was calculated as a ration of final to initial nematode populations. Galling and egg mass indices on roots were scored on a scale of 0–5. Data was analyzed using ANOVA, SAS software version 9.2, and significant means were separated using Tukey's Honest Significant Difference at 5% (P ≤ 0.05). The treatments were significantly (P ≤ 0.05) consistent in reducting plant disease and increasing growth parameters under greenhouse and field tests. <em>Colletotrichum nigrum</em> + <em>Lantana</em> extract + Velum® significantly reduced root nematodes (&gt;21%), gall and egg mass indices (&lt;2.0 and &lt; 1.5, respectively) compared to control (5.0) and reproduction (&lt;1%.) The same treatment increased shoot height (&gt;80%), dry root weight (&gt;50 %) and dry shoot weight (&gt;70%). These findings provide an additional RKN management strategy that can be incorporated into a sustainable integrated nematode management to boost agricultural productivity.</div></div>","PeriodicalId":48589,"journal":{"name":"Rhizosphere","volume":"33 ","pages":"Article 101012"},"PeriodicalIF":3.4,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143137677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}