根际土壤微环境互作对人参皂苷含量的影响——以三年龄农用人参为例

IF 3.5 3区生物学 Q1 PLANT SCIENCES

Rhizosphere Pub Date : 2025-02-07 DOI:10.1016/j.rhisph.2025.101023

Zhefeng Xu , Yuqiu Chen , Rui Liu , Yibing Wang , Chunshuo Liu , Jing Fang , Qinghe Zhang , Tao Zhang , Changbao Chen

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引用次数: 0

摘要

在农田种植人参（Panax ginseng C. A. Meyer）是人参栽培的主要方法，但根际土壤微环境与人参根中人参皂苷积累的相互作用机制尚不清楚。因此，对3年生农用人参在5月和10月两个生育期的人参皂苷含量和根际土壤微环境进行了研究。结果表明：根际土壤微环境容重、pH、碱性氮和有机质含量显著降低；根际碳循环相关酶（淀粉酶、转化酶、纤维素酶等）、氮循环相关酶（脲酶、尿酸酶、硝酸还原酶等）、磷循环相关酶（酸性磷酸酶、植酸酶）、硫循环相关酶（芳基硫酸酯酶）活性均发生显著变化。共生网络中关键微生物多样性的减少是生物水平上土壤退化的表现。人参皂苷积累显著，总皂苷含量增加42.4%。环境因素（pH、纤维素酶和阳离子交换容量）显著影响人参皂苷的积累。共现网络结果表明，真菌群落比细菌群落更容易受到环境因子的影响。与此同时，真菌群落的结构和多样性对人参皂苷积累的影响比细菌群落更显著。综合分析表明，环境因子（pH、纤维素酶、阳离子交换容量）与土壤微生物（Coprinellus、Agaricales_unclassified、Mortierella）之间的相互作用可能是影响3年生农用人参皂苷积累的关键因素。研究结果可为土壤环境改善及根据人参对肥料的需求制定相应的管理措施提供参考，有助于实现农业人参的可持续生产。

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Effect of rhizosphere soil microenvironment interaction on ginsenoside content in Panax ginseng: A case study of three-year-old agricultural ginseng

Planting ginseng (Panax ginseng C. A. Meyer) in farmland is the main method of ginseng cultivation, but the interaction between the rhizosphere soil microenvironment and the mechanism of ginsenoside accumulation in ginseng roots are still unclear. Therefore, the content of ginsenoside and rhizosphere soil microenvironment of 3-year-old agricultural ginseng at two growth periods (May and October) were studied. The results showed that the content of bulk density, pH, alkaline nitrogen and organic matter in rhizosphere soil microenvironment significantly decreased. There were significant changes in the activities of carbon cycle related enzymes (amylase, invertase, cellulase etc.), nitrogen cycle related enzymes (urease, uricase, nitrate reductase, etc.), phosphorus cycle related enzymes (acid phosphatase, phytase), and sulfur cycle related enzymes (arylsulfatase) in rhizosphere. The decrease in keystone microbial diversity in a co-occurrence network was a manifestation of soil degradation at the biological level. Ginsenosides showed significant accumulation, with a 42.4% increase in the total content of ginsenosides. Environmental factors (pH, cellulase, and cation exchange capacity) significantly affected the accumulation of ginsenosides. The results of the co-occurrence network indicated that fungal communities were more susceptible to environmental factors than bacterial communities. Meanwhile, the structure and diversity of the fungal communities had a more significant impact on the accumulation of ginsenosides compared to the bacterial community. Comprehensive analysis showed that the interaction between environmental factors (pH, cellulase, cation exchange capacity) and soil microbiome (Coprinellus, Agaricales_unclassified, Mortierella) may be the key factor affecting ginsenoside accumulation in 3-year-old agricultural ginseng. The research results provide reference for soil environment improvement and the development of appropriate management measures based on the fertilizer requirements of ginseng, which can help achieve sustainable production of agricultural ginseng.

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来源期刊

Rhizosphere Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

5.70

自引率

8.10%

发文量

155

审稿时长

29 days

期刊介绍： Rhizosphere aims to advance the frontier of our understanding of plant-soil interactions. Rhizosphere is a multidisciplinary journal that publishes research on the interactions between plant roots, soil organisms, nutrients, and water. Except carbon fixation by photosynthesis, plants obtain all other elements primarily from soil through roots. We are beginning to understand how communications at the rhizosphere, with soil organisms and other plant species, affect root exudates and nutrient uptake. This rapidly evolving subject utilizes molecular biology and genomic tools, food web or community structure manipulations, high performance liquid chromatography, isotopic analysis, diverse spectroscopic analytics, tomography and other microscopy, complex statistical and modeling tools.