Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology最新文献

{"title":"Study of the Mechanism of Gamma-Aminobutyric Acid Inhibitory Effect on the Myotube Formation in Cell Culture","authors":"A. R. Tokmakova,&nbsp;G. V. Sibgatullina,&nbsp;K. R. Gilizhdinova,&nbsp;A. I. Malomouzh","doi":"10.1134/S1990747823050136","DOIUrl":"10.1134/S1990747823050136","url":null,"abstract":"<p>Gamma-aminobutyric acid (GABA) is commonly considered as a signaling molecule in the synapses of the central nervous system, where it plays the role of the main inhibitory neurotransmitter in the mature brain and participates in the process of neurogenesis. Recently, data have been obtained indicating that GABA may also be involved in the early stages of skeletal muscle tissue development. In the present study, performed on rat myocyte culture, the effect of exogenous GABA on the process of fusion of myocytes into myotubes was investigated by analyzing such a morphometric indicator as the “fusion index”. The addition of the amino acid to the culture led to a significant concentration-dependent inhibition (up to a complete cessation) of the formation of myotubes. GABA_A receptors and GABA transporters (GAT-2) were considered among possible proteins capable of mediating the effect of amino acids. Evidence of the presence of these proteins on cultured cells was obtained using immunohistochemical methods. The blockade of GABA receptors by gabazine did not affect the fusion index in any way, and GABA continued to exert inhibitory effect in its presence. Inhibition of GABA transporters by nipecotic acid reduced the myocyte fusion index; however, the GABA effect was no longer present under the action of the transporter blocker. The data obtained are consistent with the hypothesis about the participation of the amino acid GABA at the early stages of skeletal muscle development; it suggests that the inhibitory effect of the exogenous amino acid may be due to an increase in its concentration in the sarcoplasm, since both the addition of a GABA transporter blocker and an increase in the extracellular concentration of GABA negatively affect the formation of myotubes.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"17 4","pages":"319 - 324"},"PeriodicalIF":1.1,"publicationDate":"2023-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138565818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of Nitric Oxide and Hydrogen Sulfide in Neuronal and Glial Cell Death in Neurodegenerative Processes","authors":"S. V. Rodkin,&nbsp;C. D. Nwosu","doi":"10.1134/S1990747823050069","DOIUrl":"10.1134/S1990747823050069","url":null,"abstract":"<p>Neurodegeneration is a complex progressive pathological process leading to the neuronal death, which is induced by various external and internal factors. Neurodegenerative diseases, injuries of the central and peripheral nervous system, mental disorders, and a number of other pathological conditions, accompanied by functional and structural degradation of neurons and their death, is a serious problem in the global healthcare system, as due to these diseases millions of people around the world become disabled or die every year. The situation is complicated by the lack of selective, clinically effective neuroprotective drugs. It has been shown that nitric oxide (NO) and hydrogen sulfide (H₂S) are actively involved in neurodegeneration and cell death of neurons and glia, but their role is not completely clear. This review considers NO- and H₂S-dependent signaling mechanisms underlying the pathogenesis of neurodegenerative processes. The prospects for further studies of the role of NO and H₂S in the nervous tissue under conditions of pathological conditions associated with neurodegeneration are considered.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"17 4","pages":"223 - 242"},"PeriodicalIF":1.1,"publicationDate":"2023-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138565920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of the Spectral Characteristics of the Plant Cell Surface: Occurrence of Azulenes and Biogenic Amines","authors":"V. V. Roshchina,&nbsp;V. A. Yashin,&nbsp;A. R. Kunyev","doi":"10.1134/S1990747823050070","DOIUrl":"10.1134/S1990747823050070","url":null,"abstract":"<p>The spectral characteristics of plant surface cells of different evolutionary levels, from unicellular (diatom algae and spores of horsetails and ferns) to multicellular (woody and herbaceous species) organisms, have been studied. It was shown that the surface layers of the cuticle and cell wall of some analyzed plants included antioxidants – blue pigments azulenes. Using histochemical methods, it has been discovered that here neurotransmitter compounds (biogenic amines) are present in the excretions on the entire surface or are released from specialized secretory structures of leaves. Under conditions of high salt concentration, dopamine and histamine are secreted, which is blocked by the addition of exogenous azulene and proazulene grosshemine. We suggest that the azulene-containing surface protects from the formed reactive oxygen species and toxic biogenic amines in high concentrations.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"17 4","pages":"276 - 285"},"PeriodicalIF":1.1,"publicationDate":"2023-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138569719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Human Chorionic Gonadotropin on Differentiation and Functional Activity of Myeloid-Derived Suppressor Cells","authors":"K. Yu. Shardina,&nbsp;V. P. Timganova,&nbsp;M. S. Bochkova,&nbsp;S. V. Uzhviyuk,&nbsp;S. A. Zamorina","doi":"10.1134/S1990747823050094","DOIUrl":"10.1134/S1990747823050094","url":null,"abstract":"<p>The effect of recombinant human chorionic gonadotropin (hCG) at pregnancy-appropriate concentrations (10 and 100 IU/mL) on differentiation and functional activity of myeloid-derived suppressor cells (MDSCs) was studied. The object of the study was isolated CD11b⁺ cells that were converted to the MDSC phenotype by two-step activation with GM-CSF cytokines, IL1β and lipopolysaccharide (LPS). After a week of cultivation, the total MDSC level was determined considering the subpopulations M-MDSC and PMN-MDSC, the expression of arginase-1 (Arg1) and indoleamn-2,3-dioxydiogenase (IDO) in these cells, as well as the cytokine profile in cell culture supernatant. It was shown that hCG increased the total number of MDSCs, and its lower concentration (10 IU/mL) contributed to the differentiation of the M-MDSC subpopulation. hCG did not affect the expression of IDO expression in MDSCs, but there was a tendency to increase IDO expression under the influence of hCG at a concentration of 10 IU/mL. CD11b⁺ cells converted to the MDSC phenotype had a low Arg 1 content, making it impossible to evaluate the effect of the hormone on the expression of this enzyme. Evaluation of the cytokine profile by multiplex analysis showed that hCG did not modulate cytokine production in the culture of CD11b⁺ cells converted the MDSC phenotype. This is the first time that hCG has been shown to induce differentiation of MDSCs.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"17 4","pages":"332 - 339"},"PeriodicalIF":1.1,"publicationDate":"2023-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138566155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Sex Hormones on the ABCG2 Transport Protein in Caco-2 Cells","authors":"A. A. Slepnev,&nbsp;Yu. V. Abalenikhina,&nbsp;N. M. Popova,&nbsp;A. V. Shchulkin,&nbsp;E. N. Yakusheva","doi":"10.1134/S1990747823050100","DOIUrl":"10.1134/S1990747823050100","url":null,"abstract":"<p>ABCG2 protein (breast cancer resistance protein, BCRP) is an efflux transmembrane protein involved in the transport of endogenous and exogenous substances, as well as in the development of tumor resistance to chemotherapy. In this work, the effects of sex hormones progesterone, estradiol, and testosterone on the relative content of ABCG2 in Caco-2 cells was evaluated. The role of orphan receptors (farnasoid X receptor (FXR), constitutive androstane receptor (CAR), pregnane X receptor (PXR), and liver X receptor subtype alpha (LXRα) in the effects of sex hormones was also studied. The content of ABCG2 was estimated by the Western blot technique. Hormones were used at concentrations of 1, 10, and 100 μM; exposure duration was 24 h. All hormones at all concentrations caused an increase in the content of ABCG2. Inhibition of PXR and FXR prevented an increase in ABCG2 levels induced by progesterone. Suppression of CAR and PXR partially reduced the expression of ABCG2 caused by estradiol, as compared to exposure to estrogen alone, but still the level of the transporter exceeded the control. Inhibition of PXR and FXR attenuated the inducing effect of testosterone; however, the level of the transporter exceeded the control. Thus, it was shown that all sex hormones at concentrations 1, 10, and 100 μM increased the content of ABCG2. CAR and PXR participated in the action of estradiol, while FXR and PXR participated in the action of testosterone and progesterone.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"17 4","pages":"293 - 300"},"PeriodicalIF":1.1,"publicationDate":"2023-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138566047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Hydrogen Sulphide on Spontaneous Contractions of the Rat Jejunum: Role of KV, KCa, and Kir Channels","authors":"D. M. Sorokina,&nbsp;I. F. Shaidullov,&nbsp;D. Buchareb,&nbsp;F. G. Sitdikov,&nbsp;G. F. Sitdikova","doi":"10.1134/S1990747823060089","DOIUrl":"10.1134/S1990747823060089","url":null,"abstract":"<p>In this study we investigated the role of voltage dependent (K_V), Ca²⁺-activated (K_Ca), and inward rectifier (K_ir) potassium channels in the effects of hydrogen sulfide donor (H₂S) sodium hydrosulfide (NaHS) on spontaneous contractile activity of rat jejunum. It was shown that NaHS dose-dependently (10–500 μM) reduced the tonus of the preparation, as well as the amplitude and frequency of spontaneous contractions of jejunum preparations under isometric conditions; the half-maximal effective concentration (EC₅₀) of the inhibitory effect of NaHS on the amplitude of contractions was 165 μM. The blocker of K_V channels 4-AP (200 μM) caused an increase in the amplitude of spontaneous contractions. NaHS (200 μM) decreased the amplitude and frequency of spontaneous activity of the preparation in the presence of 4-AP as well as in the control, and the effect on basal tonus was less pronounced. Blockers of large conductance K_Ca channels (BK), non-specific TEA (3 mM) and specific paxillin (1 μM), increased the amplitude of spontaneous contractions, while the depressing effect of NaHS was completely preserved. The selective blocker of small conductance K_Ca channels (SK) NS8593 (4 μM) did not affect the tonus of the preparation and the parameters of spontaneous contractions; it did not prevent the effect of NaHS. The activator of K_ATP channels diazoxide (100 μM) caused a decrease in the basal tonus of the preparation, as well as the amplitude and frequency of spontaneous contractions. Diazoxide and the K_ATP channel blocker glibenclamide (50 μM) prevented the effect of NaHS on the tonus of the preparation. BaCl₂, the K_ir channel blocker (30 μM), caused an increase in the amplitude of spontaneous contractions and prevented the development of the NaHS inhibitory effects on the frequency and amplitude of spontaneous contractions; the decrease in tonus was less pronounced than in the control. Thus, a decrease in the basal tonus of the rat jejunum preparation under the action of the H₂S donor was associated with activation of K_ir channels, including K_ATP channels, whereas the effect of H₂S on amplitude and frequency was mediated by an increase in Ba²⁺-sensitive conductivity.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"17 4","pages":"301 - 310"},"PeriodicalIF":1.1,"publicationDate":"2023-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138621152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Role of Post-Translational Protein Acetylation and Deacetylation in the Apoptosis of Neurons of the Peripheral Nervous System","authors":"V. A. Dzreyan,&nbsp;S. V. Demyanenko","doi":"10.1134/S199074782306003X","DOIUrl":"10.1134/S199074782306003X","url":null,"abstract":"<p>Neurotrauma is among the main causes of human disability and mortality. However, the mechanisms that mediate the survival and death of cells in the peripheral nervous system are still not fully understood. The transcription factors p53 and E2F1 are the master regulators of basic cellular functions, including DNA repair, cell cycle, metabolism, and apoptosis. Overexpression of p53 and E2F1, shown in a number of experimental models of peripheral nerve injury, suggests an important role of these proteins in the pathogenesis of neurotrauma. This review discusses the epigenetic mechanisms of p53 and E2F1 activation and regulation, which may contribute to the survival or death of neurons and glial cells after traumatic injury. Prospects for further studies of the mechanisms of regulation of the p53 and E2F1 proteins, including those involving histone deacetylases, for the development of neuroprotectors are considered.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"17 4","pages":"249 - 263"},"PeriodicalIF":1.1,"publicationDate":"2023-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138566055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Current Trends in the Application of Stem Cells and Their Derivatives in Animal Sperm Cryopreservation","authors":"M. A. Tambovsky,&nbsp;A. M. Aimaletdinov,&nbsp;E. Yu. Zakirova","doi":"10.1134/S1990747823050112","DOIUrl":"10.1134/S1990747823050112","url":null,"abstract":"<p>Sperm cryopreservation is an important part of preserving the germ cells of various organisms. However, when gametes are frozen, various damage often occur, which have a significant impact in artificial insemination. After thawing, spermatozoa usually have ultrastructural, biochemical, and functional changes such as damage of cell membrane and chromatin and oxidative stress. Since spermatozoa have a limited capacity for biosynthetic activity, they have a low capacity for regeneration. The current trend is to improve sperm cryopreservation using natural extracellular vesicles and stem cells. Extracellular vesicles and stem cells have potential regenerative effects because they contain various bioactive molecules to affect sperm repair. The present review focuses on current strategies to improve sperm health after cryopreservation. In particular, this review describes the results of studies on the use of extracellular vesicles and stem cells as cryoprotectants during sperm freezing and thawing.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"17 4","pages":"243 - 248"},"PeriodicalIF":1.1,"publicationDate":"2023-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138569859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of the Toxic Effect of 2-(Chlorodinitromethyl)-4-Methoxy-6-(4-Methylpiperazine-1-yl)-1,3,5-Triazine by Respiratory Activity of Lymphocytes","authors":"P. V. Iliasov,&nbsp;L. V. Limareva,&nbsp;A. I. Sizova,&nbsp;V. A. Zalomlenkov,&nbsp;A. P. Kuricyna","doi":"10.1134/S1990747823050057","DOIUrl":"10.1134/S1990747823050057","url":null,"abstract":"<p>A method for evaluation of metabolic characteristics of intact cells based on electrochemical registration of their respiratory activity was used to monitor a reaction of lymphocytes to a potential pharmacological agent, 2-(chlorodinitromethyl)-4-methoxy-6-(4-methylpiperazin-1-yl)-1,3,5-triazine. The method ensured an estimation of cytotoxicity of the test compound and made it possible to determine its minimum toxic concentrations for human lymphocytes. It was shown that the obtained results agree with the data of the reference method, MTT-based cell viability assay.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"17 4","pages":"269 - 275"},"PeriodicalIF":1.1,"publicationDate":"2023-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138610211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of Piezo1 Channels in Mechano-Anabolic Coupling in Rat Soleus Muscle","authors":"K. V. Sergeeva,&nbsp;S. A. Tyganov,&nbsp;V. E. Kalashnikov,&nbsp;B. S. Shenkman,&nbsp;T. M. Mirzoev","doi":"10.1134/S1990747823050082","DOIUrl":"10.1134/S1990747823050082","url":null,"abstract":"<p>It is known that activation of protein synthesis and hypertrophy of muscle fibers in response to mechanical stress is realized through an anabolic mTORC1-dependent signaling pathway. However, mechanosensors through which a mechanical signal can be perceived and further transmitted to the mTORC1-dependent signaling pathway (mechanotransduction) are poorly identified. Mechanically activated (MA) ion channels are candidates for the role of such sarcolemmal mechanosensors. In this regard, the aim of this study was to investigate the potential role of MA channels (Piezo1) in the activation of the mTORC1-dependent pathway in the isolated rat soleus muscle in response to mechanical stress. Wistar rats were divided into 3 groups: (1) “Control” (animal muscles were not exposed to MA channel inhibitor or Piezo1 channel activator); (2) “Gadolinium” (animal muscles were incubated with gadolinium chloride, MA channel inhibitor), and (3) “Yoda” (animal muscles were incubated with Yoda1, Piezo1 MA channel activator). In rats from each group, <i>m. soleus</i> was isolated from the left limb and incubated in the appropriate solution without mechanical stress in the form of a series of stretching (“Rest”); <i>m. soleus</i> from the right limb was subjected to a series of stretching (“Stretch”) and then incubated in the appropriate solution. Phosphorylation of mTORC1 targets (p70S6K, rpS6, and 4E-BP1) in rat <i>m. soleus</i> was determined by PAAG electrophoresis and immunoblotting. A series of passive stretches of isolated <i>m. soleus</i> led to an increase in the phosphorylation of p70S6K, its substrate rpS6, and 4E-BP1 by 38.5, 168 and 112%, respectively, compared to the muscle that was not subjected to mechanical stress. Incubation of the muscles with gadolinium completely prevented the activation of mTORC1 markers caused by a series of stretches. Incubation of <i>m. soleus</i> in a solution with Yoda1 resulted in a decrease in the mechano-dependent phosphorylation of p70S6K, rpS6 and 4E-BP1 compared to the muscle that was not exposed to Yoda1. Thus, the methodological approach used in this work did not reveal the participation of Piezo1 in mechano-anabolic coupling in rat <i>m. soleus</i>.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"17 4","pages":"286 - 292"},"PeriodicalIF":1.1,"publicationDate":"2023-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138565808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}