Distribution of Progesterone Receptors and the Membrane Component of the Progesterone Receptor in Various Organs and Tissues of Male and Female Rats

IF 1.1 Q4 CELL BIOLOGY
A. D. Dmitrieva, I. A. Morozov, A. M. Karkhov, P. M. Rubtsov, O. V. Smirnova, T. A. Shchelkunova
{"title":"Distribution of Progesterone Receptors and the Membrane Component of the Progesterone Receptor in Various Organs and Tissues of Male and Female Rats","authors":"A. D. Dmitrieva,&nbsp;I. A. Morozov,&nbsp;A. M. Karkhov,&nbsp;P. M. Rubtsov,&nbsp;O. V. Smirnova,&nbsp;T. A. Shchelkunova","doi":"10.1134/S1990747825700047","DOIUrl":null,"url":null,"abstract":"<p>Progesterone regulates reproductive processes and affects many functions of various non-reproductive organs. Its effects in mammals and humans are mediated by nuclear (nPRs) and membrane progesterone receptors (mPRs). The action of progesterone through different types of receptors differs significantly and also has tissue-specific features. The expression of known types and subtypes of progesterone receptors in tissues of male and female rats has not been sufficiently studied. The aim of this work was to investigate the expression of five mPRs genes and nPRs gene, as well as membrane component of progesterone receptor PGRMC1 in reproductive organs and in 17 non-reproductive tissues of male and female rats by reverse transcription followed by real-time PCR. A high level of nPRs gene expression has been found not only in reproductive organs of female rats (uterus, ovary, mammary glands), but also in seminal vesicles of male rats, in the brain and trachea of both sexes, in blood vessels and in the pancreas of females. The highest level of expression of mPRs genes of all subtypes was in the testes, while expression of the gene encoding nPRs was practically undetectable in them. Expression of genes encoding mPRs was also detected in the liver and spleen of male and female rats, while expression of the gene encoding nPRs was at the background level. No expression of nPRs, mPRs and membrane component of progesterone receptor (PGRMC1) genes was detected in muscle, and its level was very low in heart in animals of both sexes. Nuclear and membrane receptor mRNA expression in non-reproductive tissues in rats has been shown to be sex-dependent. Transcription of nPRs and three subtypes of mPRs (α, β, δ) was predominant in females, and two subtypes of mPRs (γ, ε) were predominant in males. Evidence for the presence of progesterone receptors in tissues not involved in reproduction supports the action of progesterone on these organs. The high mRNA levels of various progesterone receptors in male rat tissues such as pancreas, lung, kidney, and trachea suggest an important physiological role of progestins not only in females but also in males, which is still poorly understood. The paper also discusses the known functions of progesterone receptors in the tissues studied.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"18 1 supplement","pages":"S33 - S47"},"PeriodicalIF":1.1000,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","FirstCategoryId":"2","ListUrlMain":"https://link.springer.com/article/10.1134/S1990747825700047","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Progesterone regulates reproductive processes and affects many functions of various non-reproductive organs. Its effects in mammals and humans are mediated by nuclear (nPRs) and membrane progesterone receptors (mPRs). The action of progesterone through different types of receptors differs significantly and also has tissue-specific features. The expression of known types and subtypes of progesterone receptors in tissues of male and female rats has not been sufficiently studied. The aim of this work was to investigate the expression of five mPRs genes and nPRs gene, as well as membrane component of progesterone receptor PGRMC1 in reproductive organs and in 17 non-reproductive tissues of male and female rats by reverse transcription followed by real-time PCR. A high level of nPRs gene expression has been found not only in reproductive organs of female rats (uterus, ovary, mammary glands), but also in seminal vesicles of male rats, in the brain and trachea of both sexes, in blood vessels and in the pancreas of females. The highest level of expression of mPRs genes of all subtypes was in the testes, while expression of the gene encoding nPRs was practically undetectable in them. Expression of genes encoding mPRs was also detected in the liver and spleen of male and female rats, while expression of the gene encoding nPRs was at the background level. No expression of nPRs, mPRs and membrane component of progesterone receptor (PGRMC1) genes was detected in muscle, and its level was very low in heart in animals of both sexes. Nuclear and membrane receptor mRNA expression in non-reproductive tissues in rats has been shown to be sex-dependent. Transcription of nPRs and three subtypes of mPRs (α, β, δ) was predominant in females, and two subtypes of mPRs (γ, ε) were predominant in males. Evidence for the presence of progesterone receptors in tissues not involved in reproduction supports the action of progesterone on these organs. The high mRNA levels of various progesterone receptors in male rat tissues such as pancreas, lung, kidney, and trachea suggest an important physiological role of progestins not only in females but also in males, which is still poorly understood. The paper also discusses the known functions of progesterone receptors in the tissues studied.

Abstract Image

黄体酮受体在雌雄大鼠各器官组织中的分布及黄体酮受体膜组分
黄体酮调节生殖过程,影响各种非生殖器官的许多功能。它在哺乳动物和人类中的作用是由核(nPRs)和膜孕酮受体(mPRs)介导的。黄体酮通过不同类型受体的作用有显著差异,并具有组织特异性。已知的黄体酮受体类型和亚型在雌雄大鼠组织中的表达尚未得到充分的研究。本研究通过反转录和实时荧光定量PCR技术,研究了雄性和雌性大鼠生殖器官和17种非生殖组织中5个mPRs基因和nPRs基因以及孕激素受体PGRMC1膜组分的表达。不仅在雌性大鼠的生殖器官(子宫、卵巢、乳腺)中发现了高水平的nPRs基因表达,而且在雄性大鼠的精囊、两性大鼠的大脑和气管、雌性大鼠的血管和胰腺中发现了高水平的nPRs基因表达。所有亚型的mPRs基因在睾丸中表达水平最高,而编码nPRs的基因在睾丸中几乎没有表达。在雄性和雌性大鼠的肝脏和脾脏中也检测到编码mPRs的基因的表达,而编码nPRs的基因的表达处于背景水平。在肌肉中未检测到nPRs、mPRs和孕激素受体膜组分(PGRMC1)基因的表达,其在雌雄动物心脏中的表达水平极低。核和膜受体mRNA在大鼠非生殖组织中的表达已被证明是性别依赖的。nPRs和3种mPRs亚型(α、β、δ)的转录在雌性中占优势,而2种mPRs亚型(γ、ε)的转录在雄性中占优势。黄体酮受体存在于非生殖组织的证据支持黄体酮对这些器官的作用。在雄性大鼠胰腺、肺、肾和气管等组织中,各种孕激素受体的mRNA水平较高,这表明孕激素不仅在雌性中,而且在雄性中也具有重要的生理作用,这一点目前尚不清楚。本文还讨论了孕激素受体在研究组织中的已知功能。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
1.40
自引率
0.00%
发文量
28
期刊介绍: Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology   is an international peer reviewed journal that publishes original articles on physical, chemical, and molecular mechanisms that underlie basic properties of biological membranes and mediate membrane-related cellular functions. The primary topics of the journal are membrane structure, mechanisms of membrane transport, bioenergetics and photobiology, intracellular signaling as well as membrane aspects of cell biology, immunology, and medicine. The journal is multidisciplinary and gives preference to those articles that employ a variety of experimental approaches, basically in biophysics but also in biochemistry, cytology, and molecular biology. The journal publishes articles that strive for unveiling membrane and cellular functions through innovative theoretical models and computer simulations.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信