Influence of Membrane Curvature on the Energy Barrier of Pore Formation

IF 1.1 Q4 CELL BIOLOGY
R. J. Molotkovsky, P. V. Bashkirov
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引用次数: 0

Abstract

Formation of through conducting defects—pores—in the lipid bilayer affects many processes in living cells and can lead to strong changes in cellular metabolism. Pore formation is a complex topological rearrangement and occurs in several stages: first, a hydrophobic through pore is formed, then it is reconstructed into a hydrophilic pore with a curved edge, the expansion of which leads to membrane rupture. Pore formation does not occur spontaneously, since it requires significant energy costs associated with membrane deformation. The evolution of the system is associated with overcoming one or two energy barriers, the ratio of their heights affects the stability of the pore and the probability of its formation. We study the effect of membrane curvature on the height of the energy barrier for the transition of a pore to a metastable hydrophilic state. We apply the theory of elasticity of lipid membranes and generalize the model of pore formation in flat membranes to the case of arbitrary curvature. We show that the barrier for pore formation decreases by 8kBT when the radius of curvature decreases from 1000 to 10 nm, which facilitates the formation of a metastable pore. Our results are consistent with experimental data and can be used to model complex processes occurring in curved regions of living cell membranes.

Abstract Image

膜曲率对孔隙形成能垒的影响
脂质双分子层中通过传导缺陷-孔的形成影响活细胞的许多过程,并可导致细胞代谢的强烈变化。孔的形成是一个复杂的拓扑重排过程,发生在几个阶段:首先形成疏水通孔,然后重建成具有弯曲边缘的亲水孔,其膨胀导致膜破裂。孔隙的形成不是自发发生的,因为它需要与膜变形相关的大量能量成本。体系的演化与克服一个或两个能垒有关,它们的高度比影响着孔隙的稳定性和形成的概率。我们研究了膜曲率对孔过渡到亚稳态亲水态的能垒高度的影响。我们应用脂质膜的弹性理论,将平面膜的孔隙形成模型推广到任意曲率的情况。我们发现,当曲率半径从1000 nm减小到10 nm时,孔隙形成屏障降低了8kBT,有利于亚稳孔隙的形成。我们的结果与实验数据一致,可用于模拟发生在活细胞膜弯曲区域的复杂过程。
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来源期刊
CiteScore
1.40
自引率
0.00%
发文量
28
期刊介绍: Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology   is an international peer reviewed journal that publishes original articles on physical, chemical, and molecular mechanisms that underlie basic properties of biological membranes and mediate membrane-related cellular functions. The primary topics of the journal are membrane structure, mechanisms of membrane transport, bioenergetics and photobiology, intracellular signaling as well as membrane aspects of cell biology, immunology, and medicine. The journal is multidisciplinary and gives preference to those articles that employ a variety of experimental approaches, basically in biophysics but also in biochemistry, cytology, and molecular biology. The journal publishes articles that strive for unveiling membrane and cellular functions through innovative theoretical models and computer simulations.
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