Beni-Suef University Journal of Basic and Applied Sciences最新文献

{"title":"Anticancer potential of four triterpenoids against NCI-60 human tumor cell lines","authors":"Beatrice Njeri Irungu,&nbsp;Mary Nyangi,&nbsp;Fidelis Toloyi Ndombera","doi":"10.1186/s43088-024-00507-8","DOIUrl":"10.1186/s43088-024-00507-8","url":null,"abstract":"<div><h3>Background</h3><p>The burden of cancer incidences and mortality is rapidly increasing worldwide resulting in an increased demand for new therapies. Secondary metabolites extracted from medicinal plants have significantly contributed toward discovery of new cancer therapies some of which are in clinical use. In this study, anticancer potential of four triterpenoids, namely oleanonic acid (<b>EK-2</b>), 3-<i>epi</i>-oleanolic acid (<b>EK-8</b>), 1,2,3,22,23-pentahydroxy-2,6,10,15,19,23-hexamethyl-6,10,14,18-tetracosatetraene (<b>EK-4</b>) and 2,3,22,23-tetrahydroxy-2,6,10,15,19,23-hexamethyl-6,10,14,18-tetracosatetraene (<b>EK-9</b>), extracted from <i>Ekebergia capensis</i> Sparrm root bark was evaluated.</p><h3>Results</h3><p>We employed CLC-Pred to initially evaluate cytotoxicity of previously isolated compounds in silico where predictions revealed high probability of bioactivity. The compounds were then submitted to the National Cancer Institute (NCI), Developmental Therapeutics Program, for bioactivity evaluation against NCI-60 human tumor cell lines. The four compounds demonstrated a range of potencies at a concentration of 10 µM. The results revealed that <b>EK-9</b> was the most potent with mean growth percent of 32.84 and cases of lethality (negative growth percent) against two leukemia cell lines (HL-60 (TB) and RPMI-8226) and HT29 (colon cancer) and SK-MEL-5 (melanoma). This molecule was further evaluated in a five-dose assay where notable growth inhibition against leukemia cells, HL-60 (TB), RPMI-8226 and K-562 was observed with growth inhibitory activity (GI₅₀) values of 3.10, 3.74 and 5.07 µM, respectively. In addition, total growth inhibition was observed at 11.2 μM and 18.9 μM for HL-60 (TB) and RPMI-8226 cells, respectively, partly accounting for the negative growth percent.</p><h3>Conclusion</h3><p>The study has demonstrated anticancer properties of the four triterpenoids with compound <b>EK-9</b> being the most potent overall having selective bioactivity in leukemia and breast cancer cells. Further studies focusing on elucidating its mechanism of action will be useful in exploration of the therapeutic potential of triterpenoids in general.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"13 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-024-00507-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141187290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Estimating the expression levels of genes controlling biofilm formation and evaluating the effects of different conditions on biofilm formation and secreted aspartic proteinase activity in Candida albicans and Saccharomyces cerevisiae: a comparative study","authors":"Shaimaa S. Sobieh,&nbsp;Rowida G. Elshazly,&nbsp;Sahar A. Tawab,&nbsp;Sanaa S. Zaki","doi":"10.1186/s43088-024-00504-x","DOIUrl":"10.1186/s43088-024-00504-x","url":null,"abstract":"<div><h3>Background</h3><p>Characterization of yeast virulence genes is an important tool for identifying the molecular pathways involved in switching yeast virulence. Biofilm formation (BF) and secreted aspartic proteinase (SAP) activity are essential virulence factors that contribute to yeast pathogenicity.</p><h3>Results</h3><p>Four <i>Candida albicans</i> and two <i>Saccharomyces cerevisiae</i> strains were tested for BF and SAP activity under optimum conditions, and the expression levels of several genes controlling BF were quantified under the optimal conditions. Biofilm formation was assessed by the microplate method at different pH values, incubation times and culture media. Similarly, SAP activity was assessed at different pH values and incubation periods. The expression levels of nine genes were determined via qRT-PCR technique. All tests were carried out in triplicate, and the values presented as the means ± standard deviations and were analysed with the SPSS programme. Only <i>C. albicans</i> (1), <i>C. albicans</i> (2) and <i>S. cerevisiae</i> 43 formed biofilms. The optimal BF was obtained after culture in sabouraud dextrose broth with 8% glucose at pH 7.5, 4 and 6, respectively, for 48h. <i>Candida albicans</i> biofilm production was more significant than that of <i>S. cerevisiae</i> 43. Moreover, the SAP activity was estimated under the optimum conditions. All yeasts showed optimal SAP activity at pH 4, but astonishingly the SAP activity of <i>S. cerevisiae</i> 44 was higher than that of <i>C. albicans</i>. The expression levels of <i>EFG1</i> and <i>ZAP1 (</i>transcription factors); <i>ALS3, HWP1</i>and <i>YWP1</i> (adhesion genes); <i>SAP1</i> and <i>SAP4</i> (aspartic proteinase) in <i>C. albicans</i> (1); and <i>FLO11</i> (adhesion gene) and <i>YPS3</i> (aspartic proteinase) in <i>S. cerevisiae</i> 43 were quantified during biofilm development at different time intervals. The expression levels of <i>EFG1, ALS3, YWP1, SAP1</i>, <i>SAP4</i>, <i>FLO11</i> and <i>YPS3</i> were upregulated at 8 h, while that of <i>ZAP1</i> was upregulated at 48 h. Only <i>HWP1</i> was downregulated.</p><h3>Conclusions</h3><p>The findings of the present study may provide information for overcoming yeast BF and pathogenicity by regulating specific genes at specific times. Additionally, this study revealed the virulence of the commensal <i>S. cerevisiae</i>, which may take the pathogenicity direction as <i>C. albicans</i>.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"13 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-024-00504-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141096241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low-grade chronic inflammation and transcriptomics: how molecular pharmacognosy can help find new natural treatment alternatives—a narrative review","authors":"Massimo Fioranelli,&nbsp;Maria Grazia Roccia,&nbsp;Bianca Przybylek,&nbsp;Francesca Romana Sconci,&nbsp;Maria Luisa Garo","doi":"10.1186/s43088-024-00506-9","DOIUrl":"10.1186/s43088-024-00506-9","url":null,"abstract":"<div><h3>Background</h3><p>The inflammatory response is fundamental to the maintenance of an organism’s physiological homeostasis. Inflammation is controlled by a series of biological events driven by specific inflammatory molecules. When inflammation is within the homeostatic range, it is considered physiological; however, it becomes pathological when it exceeds the immune system’s homeostatic control.</p><h3>Main text</h3><p>Nowadays, the treatment of chronic pathological inflammation is a challenge for pharmacology, as current anti-inflammatory drugs are intended to control acute inflammation. The aim of this narrative review was to provide an overview of the role of molecular pharmacognosy and to demonstrate how current transcriptomics techniques can make an important contribution to the study of the biological functions of natural products in the context of multicomponent/multitarget medication. From our findings, although very few studies have been identified, encouraging results for low-grade chronic inflammations (LGCIs) of various causes emerged in recent transcriptomic studies on multicomponent medicinal products composed of plant and organ extracts at the level of the skin and the musculoskeletal system (Traumeel: Tr14), the liver (Lycopodium compositum: HC-24), and the joints (Zeel-T: Ze-14).</p><h3>Conclusion</h3><p>For adequate control of LGCI, molecular pharmacognosy may be an effective approach to exploring potentially useful herbal agents that are consistent with both physiotherapeutic tradition and modern pharmacology.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"13 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-024-00506-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141084953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alleviative effect of betaine against copper oxide nanoparticles-induced hepatotoxicity in adult male albino rats: histopathological, biochemical, and molecular studies","authors":"Asmaa R. Hashim,&nbsp;Dina W. Bashir,&nbsp;Eman. Rashad,&nbsp;Mona K. Galal,&nbsp;Maha M. Rashad,&nbsp;Nasrallah M. Deraz,&nbsp;Elsayed A. Drweesh,&nbsp;S. M. El-Gharbawy","doi":"10.1186/s43088-024-00505-w","DOIUrl":"10.1186/s43088-024-00505-w","url":null,"abstract":"<div><h3>Background</h3><p>Copper oxide nanoparticles (CuO-NPs) have gained interest due to their availability, efficiency, and their cost-effectiveness. Betaine is an essential methyl donor and takes part in various physiological activities inside the body; it is found to have protective and curative effects against various liver diseases. The present study aimed to evaluate the hepatotoxic effect of CuO-NPs on adult male albino rats and the ability of betaine to alleviate such hepatotoxicity.</p><h3>Methods</h3><p>Forty adult male albino Wister rats were grouped into 4 groups (10 rats/group): group I a negative control, group II (CuO-NPs) injected with CuO-NPs intra peritoneal by insulin needle (0.5 mg/kg/day), group III (betaine + CuO-NPs) administered betaine orally by gavage needle (250 mg/kg/day 1 h before CuO-NPs) and CuO-NPs (0.5 mg/kg/day) finally, group IV (betaine) administered betaine orally by gavage needle (250 mg/kg/day) for consecutive 28 days. Blood and liver samples were gathered and processed for biochemical, molecular, histopathological, and immunohistochemical investigations.</p><h3>Results</h3><p>Group II displayed a marked rise in alanine aminotransferase (ALT), aspartate aminotransferase (AST), and malondialdehyde (MDA) levels. Furthermore, there is an excessive upregulation of the inflammatory biomarkers interleukin1 beta (IL-1β) and tumor necrosis factor-alpha (TNF-α). On the other hand, substantial reduction in glutathione (GSH) levels and significant downregulation at glutathione peroxidase (GPx) mRNA gene expression. Regarding the histopathological deviations, there were severe congestion, dilatation and hyalinization of blood vessels, steatosis, hydropic degeneration, hepatocytic necrosis, increased binucleation, degenerated bile ducts, hyperplasia of ducts epithelial lining, and inflammatory cells infiltration. Immunohistochemically, there was a pronounced immunoreactivity toward IL-1β. Luckily, the pre-administration of betaine was able to mitigate these changes. MDA was dramatically reduced, resulting in the downregulation of IL-1β and TNF-α. Additionally, there was a considerable rise in GSH levels and an upregulation of GPx. Histopathological deviations were substantially improved as diminished dilatation, hyalinization and congestion of blood vessels, hepatocytes, and bile ducts are normal to some extent. In addition, IL-1β immunohistochemical analysis revealed marked decreased intensity.</p><h3>Conclusion</h3><p>Betaine can effectively reduce the hepatotoxicity caused by CuO-NPs via its antioxidant properties and its ability to stimulate the cell redox system.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"13 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-024-00505-w","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141073681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AlphaFold-latest: revolutionizing protein structure prediction for comprehensive biomolecular insights and therapeutic advancements","authors":"Henrietta Onyinye Uzoeto,&nbsp;Samuel Cosmas,&nbsp;Toluwalope Temitope Bakare,&nbsp;Olanrewaju Ayodeji Durojaye","doi":"10.1186/s43088-024-00503-y","DOIUrl":"10.1186/s43088-024-00503-y","url":null,"abstract":"<div><p>Breakthrough achievements in protein structure prediction have occurred recently, mostly due to the advent of sophisticated machine learning methods and significant advancements in algorithmic approaches. The most recent version of the AlphaFold model, known as “AlphaFold-latest,” which expands the functionalities of the groundbreaking AlphaFold2, is the subject of this article. The goal of this novel model is to predict the three-dimensional structures of various biomolecules, such as ions, proteins, nucleic acids, small molecules, and non-standard residues. We demonstrate notable gains in precision, surpassing specialized tools across multiple domains, including protein–ligand interactions, protein–nucleic acid interactions, and antibody–antigen predictions. In conclusion, this AlphaFold framework has the ability to yield atomically-accurate structural predictions for a variety of biomolecular interactions, hence facilitating advancements in drug discovery.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"13 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-024-00503-y","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141063972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of lime kiln dust on swelling of subgrade expansive soil","authors":"Mennat-allah Eid,&nbsp;Youssef Gomaa,&nbsp;Sameh Galal","doi":"10.1186/s43088-024-00501-0","DOIUrl":"10.1186/s43088-024-00501-0","url":null,"abstract":"<div><h3>Background</h3><p>The structure of flexible or rigid pavement built on expansive subgrade soil that has a volumetric change is vulnerable to many problems that might cause failure. Pavement and construction became more durable and economical by enhancing the quality of subgrade expansive soil. Solid waste recycling has become very popular recently as a means of attaining sustainable waste management, so using lime kiln dust (LKD), which is a by-product of quick lime production, to treat expansive soil in pavement subgrades. This research describes the effect of LKD on the chemical composition, strength, and swelling of high and low-plastic clay that were extracted from two sites. The minimum LKD required for treating expansive soils was determined by using the Eades and Grim pH test. From tests, it was found that the addition of LKD increased the shrinkage limit by a range (250–500)% and decreased the plasticity and swelling potential by between (50 and 100)% of expansive subgrade soils. The strength according to CBR, increased approximately by 150% for CL soil and 800% for CH soil.</p><h3>Results</h3><p>The optimal percentage of LKD for CH soil is 6%, and for CL soil, it is 2%. The plastic limit increased by 50% for CH soil at 6% LKD. On the other hand, CL soil became non-plastic at 4% LKD. With an increase in the percentage of LKD, it led an the increase in the shrinkage limit by 500% in CH soil and 250% in CL soil. The free swell decreased by 50% in CH soil and 100% in CL soil. The swelling pressure decreased by 50% for two expansive soils. CBR increased by 800% in CH soil and by 150% in CL soil.</p><h3>Conclusion</h3><p>This work found that the addition of LKD improves the physical, chemical, and mechanical properties of expansive subgrade soil.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"13 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-024-00501-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140924793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Space/ground-borne techniques and petrographic microscopic dissection for geologic mapping in Gabal Ras Abda area, Northeastern Desert, Egypt","authors":"Mahmoud M. Salem,&nbsp;Mohamed A. Yehia,&nbsp;Ali A. Omran,&nbsp;Hassan I. El Sundoly,&nbsp;Mohammed A. Soliman,&nbsp;Karim Abdelmalik","doi":"10.1186/s43088-024-00502-z","DOIUrl":"10.1186/s43088-024-00502-z","url":null,"abstract":"<div><h3>Background</h3><p>Gabal Ras Abda area as a part of the Red Sea Mountain range, is characterized by inaccessible and rugged terrains. The exposed rock units are hardly followed in the field because of the rigid topography. Thus, the present work proposes and develops an integrated approach to map the exposed rock units and extract the geologic structures using satellite imagery data followed by both field and petrographic verification, saving time, efforts and cost.</p><h3>Results</h3><p>To achieve the target, both the measured spectral signature curves with Landsat-8 and Sentinel-2A data were used to develop and create the most enhanced Band Ratios and Principal Components for lithological discrimination and mapping which were (((Band7 + Band 4)/(Band 7), (Band 2)/(Band 2 + Band 5) and (Band 5) in RGB) and ((Band 9 + Band 11 + Band 12)/(Band 1), (Band 4 − Band 2) and (Band 11/Band 6) + (Band 6) in RGB)) with Principal Component Bands ((PC1, PC2 and PC3 in RGB) and (PC3, PC2 and PC1 in RGB)), respectively. Also, georeferenced Google Earth Pro, panchromatic band of Landsat-8 and ALOS PALSAR Digital Elevation Model images were used to extract the structural lineaments. Geologic, petrographic and field structural studies were emphasized the remote sensing results, indicating that the main rock types cropped out in Ras Abda area from the oldest to the youngest are older granitoids (quartz-diorites, tonalites and granodiorites), Dokhan volcanics (andesites, rhyodacites, rhyolites and their related tuffs), younger gabbros, younger granites (monzogranites, syenogranites and alkali-feldspar granites), post-granite dykes and offshoots (acidic, microgranitic and basic types) and Phanerozoic sedimentary rocks. Also, the study emphasized that the E-W trend is the main structural trend controlling the investigated area followed by WNW-ESE and NE-SW directions.</p><h3>Conclusions</h3><p>The results of remote sensing achieved compliance with the geologic, petrographic and structural investigation through distinctly differentiating the different rocks and extracting the lineaments, indicating the accuracy of the remote sensing results and emphasizing their importance and effective role in producing a precise and highly accurate geologic map.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"13 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-024-00502-z","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140906945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative study of the reptilian cornea's microstructure","authors":"Zeinab Abdelftah,&nbsp;Ahmed Ragab,&nbsp;Rasha E. Abo-Eleneen,&nbsp;Abdelaziz S. Abuelsaad,&nbsp;Ahlam M. EL-Bakry","doi":"10.1186/s43088-024-00485-x","DOIUrl":"10.1186/s43088-024-00485-x","url":null,"abstract":"<div><h3>Background</h3><p>The cornea of various vertebrate is considered a major part in the glop, which acts as a powerful lens, providing a sharp retinal image, and meantime acts as an excellent defensor for other corneal layers.</p><h3>Results</h3><p>The four reptilian families [Scincidae (<i>Chalcides ocellatus</i>); Chamaeleontidae (<i>Chameleon chameleon</i>); Cheloniidae (<i>Chelonia mydas</i>) and Testudiniae (<i>Testudo kleinmanni</i>)] investigated in the current study were gathered across Egypt. After being taken out of the orbit, the cornea under inquiry was ready for light, specific stain and scanning electron microscopy. The epithelium, stroma, and endothelium are the three corneal layers that are common to all four species of reptiles. All other species lack Bowmen's and Descemet's membranes, with the exception of <i>C. ocellatus</i> and <i>T. kleinmanni</i>. The latter layers of <i>Chalcides ocellatus</i> display a strong affinity for Periodic Acid Schiff stain. Epithelial cells with a variety of forms, from hexagonal to atypical polygonal cells, cover the outer corneal surface. These epithelial cells are coated in short microplicae, microvilli, and microholes of varied diameters. There are a few blebs scattered around their surface.</p><h3>Conclusion</h3><p>The current study concluded that various habitats have various significant effects on the cornea's microstructure characteristics and its physiological trends to accommodate different environmental surroundings.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"13 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-024-00485-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140895167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interaction between HOTTIP, H19, and HOTAIR long noncoding RNAs and miRNA-152 in cases of HCC caused by HCV infection","authors":"Rady E. El-Araby,&nbsp;Fawzy Roshdy,&nbsp;Mariam Zaghloul,&nbsp;Ahmed A. E. Saad,&nbsp;Maha H. Morsi,&nbsp;Wafaa M. Radwan,&nbsp;Rana M. Adel,&nbsp;Sara H. Elshafiey,&nbsp;Yasmine Elhusseny,&nbsp;Reham F. Othman,&nbsp;Hamed Helal,&nbsp;Doha E. Hassanein,&nbsp;Hany A. Elghobary","doi":"10.1186/s43088-024-00499-5","DOIUrl":"10.1186/s43088-024-00499-5","url":null,"abstract":"<div><h3>Background</h3><p>Liver cancer (hepatocellular carcinoma “HCC”) remains a significant health issue without prompt detection and appropriate prevention. By interacting with each other, long noncoding RNAs (lncRNAs) and microRNAs (miRNAs) can form gene regulatory networks. Specifically, we aim to determine whether the lncRNAs (HOTTIP, H19, and HOTAIR) and miRNA-152 interact in a significant manner in the progression of Hepatitis C virus (HCV) patients to HCC. This is followed by the question of whether these biomarkers can be used to diagnose and prognose noninvasively. We used online computational techniques to predict which miRNA group is likely to affect the lncRNAs being examined. This study involved 133 participants. 103 patients with HCV were included in the study, which was divided into two groups: Group I, with 65 cases of chronic liver disease without HCC, and Group II, with 38 cases of chronic liver disease with HCC. In addition, 30 healthy volunteers served as controls. In this study, a qRT-PCR was used to test gene expression.</p><h3>Results</h3><p>A consistent reverse correlation has been observed between lncRNAs and miRNA-152 as the disease progresses.</p><h3>Conclusion</h3><p>According to our findings, the studied biomarkers may be useful as noninvasive biomarkers for prognosis in patients with HCV Genotype 4 who develop liver cirrhosis and HCC. Many miRNAs, including miRNA-19a and miRNA-106a, may interact with lncRNAs that have been investigated in addition to miRNA-152.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"13 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-024-00499-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140895173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of single and combined administration of ubiquinone and lactoferrin on histopathology, ultrastructure, oxidative stress, and WNT4 expression gene induced by thioacetamide on hepatorenal system of adult male rats","authors":"Sohaila Abd El-Hameed,&nbsp;Iman Ibrahim,&nbsp;Walaa Awadin,&nbsp;Ahmed El-Shaieb","doi":"10.1186/s43088-024-00494-w","DOIUrl":"10.1186/s43088-024-00494-w","url":null,"abstract":"<div><h3>Background</h3><p>Hepatorenal syndrome is a life-threatening medical complication of liver cirrhosis. Hepatic cirrhosis is commonly accompanied by rapid failure of renal functions. Thioacetamide (TAA) is a potent hepatotoxin and a class 2-type carcinogen. Ubiquinone (Coq₁₀) and lactoferrin (LF) are potent antioxidants with antifibrotic and antiinflammatory effects. However, whether Coq₁₀ and LF reduce the hepatorenal injury induced by TAA remains unclear. Here, we investigated the potential protective effect of both/or Coq₁₀ and LF in ameliorating TAA-induced hepatorenal injury and the role of WNT4 gene expression in detecting TAA-induced renal injury in rats. Seventy healthy and mature male Sprague Dawley rats, weighting (200 g ± 20 g) and aging (4–6) weeks were randomly divided into seven groups (<i>n</i> = 10): control, Coq₁₀, LF, TAA, TAA + Coq₁₀, TAA + LF, and TAA + Coq₁₀ + LF. The hepatorenal injury was induced through intraperitoneal (i.p.) injection of TAA (150 mg/kg/twice/weekly) for nine weeks. Coq₁₀ (10 mg/kg/day) and LF (200 mg/kg/day) were orally administered for nine weeks.</p><h3>Results</h3><p>TAA induced marked hepatorenal damage, evident by the significant increase in the alanine aminotransferase (ALT), aspartate transaminase (AST), serum creatinine (SCr) activities, and the blood urea nitrogen (BUN) level. Besides, the significant increases in concentrations of malondialdehyde (MDA) and nitric oxide (NOx) together with significant decreases in the activities of catalase (CAT) and superoxide dismutase (SOD). The histopathological analysis of the TAA group showed obvious fibrosis, steatosis, and inflammation of the hepatic parenchyma as well as severe glomerular and tubular damage of the renal parenchyma. In addition, TAA induced marked ultrastructural alterations and up-regulation in the expression of the WNT4 gene in the kidney. Meanwhile, the biochemical, histopathological, and ultrastructural alterations were significantly decreased with significant down-regulation in the expression of WNT4 in the groups exposed to TAA and treated with Coq₁₀ and LF.</p><h3>Conclusion</h3><p>Our data suggested that Coq₁₀ and LF could have protective effects on TAA hepatorenal damage, through improving the hepatic and renal functions, reduction of oxidative stress, structural and ultrastructural alterations, besides down-regulation in the expression of WNT4.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"13 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-024-00494-w","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140826086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}