Beni-Suef University Journal of Basic and Applied Sciences最新文献

{"title":"Exploring the combined diagnostic role of serum MBL in association with renal resistive index and renal shear wave elastography in diabetic kidney disease patients","authors":"Enas Ahmed Osman,&nbsp;Khaled Mabrouk,&nbsp;Ahmed Abdelsamie Mahmoud,&nbsp;Medhat Madbouly,&nbsp;Mohamed Moharam Mohamed Hussin,&nbsp;Osama Mosbah,&nbsp;Nevine Sherif,&nbsp;Mohammed Ghanem Gaber,&nbsp;Farida M. Khanany","doi":"10.1186/s43088-025-00645-7","DOIUrl":"10.1186/s43088-025-00645-7","url":null,"abstract":"<div><h3>Background</h3><p>One of the most prevalent non-communicable diseases in the world today is diabetes. Diabetic kidney disease (DKD) is the predominant etiology of chronic kidney disease (CKD) and a major side effect of diabetes. Diagnosis of DKD is challenging and imprecise; thus, new diagnostic modalities are necessary to address this problem. The aim of the present study is to assess the role of the combined diagnostic performance of serum levels of MBL and imaging techniques, Doppler ultrasonographic renal resistive index (RRI) and shear wave elastography as a diagnostic tool for diabetic nephropathy. This case–control study included 450 type 2 diabetes mellitus (T2DM) patients, 225 without nephropathy and 225 diagnosed with diabetic nephropathy. We evaluated serum mannose-binding lectin (MBL) by enzyme-linked immunosorbent assay (ELISA) as a marker of diabetes-related inflammation, Doppler ultrasonographic renal resistive index (RRI) as a tool for assessment of blood flow in the kidney and shear wave elastography (SWE) as an imaging technique for detection of kidney fibrosis.</p><h3>Result</h3><p>The average serum MBL levels, mean RI and SWE of both kidneys were significantly higher in DKD patients compared to T2DM patients without nephropathy. The integration of the aforementioned methodologies significantly enhanced diagnostic performance, achieving a sensitivity of 96.89% and a specificity of 95.11%.</p><h3>Conclusion</h3><p>Combined serum levels of MBL and imaging techniques, Doppler ultrasonographic renal RI and shear wave elastography are promising diagnostic tools of diabetic nephropathy.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-025-00645-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144140221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bibliometric exploration of the state of the art on acrylamide and glycidamide in food safety","authors":"Yaman Ahmed Mohammed Naji,&nbsp;Mahboob Alam,&nbsp;Suhail Bashir,&nbsp;Nurul Huda Abd Kadir","doi":"10.1186/s43088-025-00639-5","DOIUrl":"10.1186/s43088-025-00639-5","url":null,"abstract":"<div><h3>Background</h3><p>Acrylamide (AA), a probable human carcinogen (Group 2A) classified by the International Agency for Research on Cancer (IARC), has emerged as a significant food safety concern worldwide. Its formation during high-temperature cooking processes, such as frying, baking, and roasting of starchy foods, has spurred extensive research efforts to understand its toxicity and develop effective mitigation strategies. This study aims to provide a comprehensive bibliometric analysis of scientific literature on AA and its metabolite, glycidamide (GA), published from 1965 to the end of 2024, focusing on the evolving trends and research emphasis related to their toxicity and mitigation in food.</p><h3>Results</h3><p>A comprehensive bibliometric analysis of 3729 Scopus articles was conducted using VOSviewer. The analysis revealed a general increase in the number of annual research publications on AA. Most studies focused on various types of toxicity associated with AA, such as neurotoxicity, cytotoxicity, and nephrotoxicity, utilizing different models. The findings indicate a growing emphasis on research to mitigate AA formation in foods as a response to its toxicity.</p><h3>Conclusions</h3><p>The study concludes that both the quality and quantity of research articles on AA have significantly improved over time, with an anticipated continued increase in the future research. This trend reflects the ongoing global concern about AA’s toxicity and the importance of developing strategies to reduce its presence in foods, which has significant implications for public health and food safety.</p><h3>Graphic abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-025-00639-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144140097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular mechanisms of signaling transduction pathways of drug target molecules with recent advancements and future perspectives for successful therapy: a comprehensive and exploratory review","authors":"Sujana Kamepalli,&nbsp;Sambamoorthy Unnam,&nbsp;Satyabrata Bhanja,&nbsp;Krishna Reddy Yeduru,&nbsp;Pratap Kumar Patra,&nbsp;Nasreen Sulthana,&nbsp;Roja Pathakota,&nbsp;Renukadevi Thota,&nbsp;Arun Kumar Sanapala","doi":"10.1186/s43088-025-00640-y","DOIUrl":"10.1186/s43088-025-00640-y","url":null,"abstract":"<div><h3>Background</h3><p>With an emphasis on current developments and potential future perspectives in therapeutic applications, this comprehensive review delves into the molecular mechanisms of signaling transduction pathways associated with pharmacological target molecules. Since these pathways are essential to the cellular response to pharmacological drugs, understanding them is necessary for developing effective treatments. The notion of cellular signaling and the range of signaling pathways that control important cellular functions are first explained in this chapter. It highlights the intricate interactions between the essential elements of signaling pathways, such as effectors, ligands, receptors, and intercellular messengers. </p><h3>Main body</h3><p>It explores the activation and subsequent signaling events of several receptors, including intracellular nuclear, t-kinase, GPCR (G-protein-coupled receptors), and ionic receptors. We reviewed innovative methods that have sped up the discovery of new drug targets and clarified intricate signaling networks, like X-ray crystallography, advanced Cryo-EM (Cryo-Electron Microscopy), high-throughput screening, allosteric modulations, Artificial intelligence, and machine learning. This review attempts to explain the clear pathway of drug target molecules for novel, focused treatments that improve patient outcomes and further the field of drug development by combining existing knowledge and suggesting future research avenues. </p><h3>Conclusion</h3><p>Prospects for the future highlight the value of interdisciplinary teams in addressing these issues, encouraging advancements in combination therapies and tailored delivery systems that can get around current drawbacks in traditional therapy. In a rapidly changing biomedical landscape, this review seeks to shed light on pathways toward more effective, customized therapies that address the underlying molecular mechanisms of disease. Ultimately, it hopes to improve patient care and therapeutic outcomes by synthesizing current knowledge and suggesting strategic research directions.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-025-00640-y","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144117782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An improved algorithm for prediction of vehicle trajectories using short-term goal-driven network","authors":"Abdalla Tawfik,&nbsp;Zaki Nossair,&nbsp;Roaa Mubarak","doi":"10.1186/s43088-025-00638-6","DOIUrl":"10.1186/s43088-025-00638-6","url":null,"abstract":"<div><h3>Background</h3><p>Prediction of vehicle trajectories is a crucial task for automated driving systems to accurately take movement actions according to the dynamic traffic environment, especially the future positions of the surrounding vehicles. Predicting how road users will behave in the future is one of the most critical and complex challenges in autonomous driving. Different data types must be combined to accomplish this task using deep learning, such as sensor readings and maps. After that, this data is used to predict a range of possible future outcomes. Existing goal-driven approaches predict the final goal and use it to complete the trajectory; this requires accurate goal prediction, which is challenging. Short-Term Goal Network (STG) addresses this challenge using multiple short-term goals instead of a single final goal.</p><h3>Results</h3><p>The proposed STG network is evaluated on the Argoverse motion forecasting dataset, and the results show significantly better performance than other goal-driven approaches. STG demonstrated a substantial improvement of over 6% in average displacement error and more than 8% in final displacement error.</p><h3>Conclusion</h3><p>This article presents an improved algorithm for predicting vehicle trajectories using short-term goals. The proposed STG algorithm is based on long short-term memory (LSTM) and attention mechanism for predicting trajectories. This work verifies that predicting more than one goal along the trajectory improves the accuracy of the predicted goal and the whole trajectory accordingly.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-025-00638-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144073632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytomedicine and green nanotechnology: enhancing glass ionomer cements for sustainable dental restorations: a comprehensive review","authors":"Dina Ezzat,&nbsp;Amr Azab,&nbsp;Ibrahim S. Kamel,&nbsp;Mohamed Abdelmonem,&nbsp;Mohamed Ahmed Ibrahim,&nbsp;Abdullah Ayad,&nbsp;Romesa Soomro,&nbsp;Mohamed Wagdy,&nbsp;Mohamed Eldebawy","doi":"10.1186/s43088-025-00633-x","DOIUrl":"10.1186/s43088-025-00633-x","url":null,"abstract":"<div><h3>Background</h3><p>Integrating phytomedicine with glass ionomer cements (GICs) offers a promising avenue for improving dental restoratives. This review comprehensively discusses the incorporation of plant extracts and derivatives into GICs to enhance their antimicrobial, mechanical, and aesthetic properties.</p><h3>Main text</h3><p>The addition of plant extracts, such as propolis, miswak, and grape seed, and the respective disinfecting derivatives of gallic acid, epigallocatechin-3-gallate, and curcumin improved both the antibacterial and mechanical properties of GICs. Furthermore, the incorporation of these derivatives is in line with current practices, which emphasize the use of natural products to minimize the use of synthetic preservatives in dental applications. In addition, this review discussed the role of these plant extracts in the green synthesis of nanoparticles (NPs), providing insights into how these sustainable approaches can fill existing gaps in dental material technology. This intersection of plant-based compounds and nanotechnology not only paves the way for innovative dental materials but also supports the broader movement toward sustainable practices in dentistry. This review revealed that the incorporation of plant extracts may significantly improve the antimicrobial properties of GICs and maintain or slightly improve their mechanical properties; however, the disparate methodologies and extraction concentrations used in these studies call for further standardized research.</p><h3>Conclusion</h3><p>This review highlights the potential of phytomedicine to optimize the performance of GICs in clinical settings and calls for further research to establish the most effective formulations.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-025-00633-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143944273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experimental study for evaluation the efficiency of some traditional and nano-composites materials consolidates to improve the archaeological limestone properties","authors":"Mohsen M. Saleh,&nbsp;M. Ezzat,&nbsp;Fatma M. Shebl","doi":"10.1186/s43088-025-00636-8","DOIUrl":"10.1186/s43088-025-00636-8","url":null,"abstract":"<div><h3>Background</h3><p>Archaeological limestone artifacts are subject to several deterioration factors that can cause harm while they are buried in soil, such as wet salt soil. Thus, one of the biggest challenges is restoring limestone artifacts that have been discovered from excavations. Understanding the nature of limestone after extraction and the resulting alterations, such as the stone’s structural instability and the high salt content of the artifacts, are prerequisites for the restorer. In 1974 AD, King Ramesses III’s gate was excavated from the ancient Heliopolis Temple in Cairo. The stones were removed from the soil and left on display outdoors at the same excavation site, where they were subject to seasonal variations in temperature and environmental changes. The main objective of the research is to select the best consolidating materials suitable for the pieces of limestone stone artifacts discovered from archaeological excavations due to their special nature, which affects them as a result of their presence in burial soil for long time. Selecting appropriate consolidating materials with appropriate characteristics was important. In order to withstand a range of environmental circumstances. The characteristics of the ancient stones at the King Ramesses III Gate site were investigated and analyzed to ascertain their true state, and their percentage of damage was calculated by contrasting them with the identical natural limestone that had not been subjected to any harmful influences. After that, experimental samples were used, and the efficacy of the treatment materials was assessed.</p><h3>Result</h3><p>Experimental study aims to evaluate the effectiveness of some traditional and nano composites materials to improving the properties of stone artifacts extracted from archaeological excavations. Three consolidating solutions were used as follows, paraloid B72 dissolved in acetone 3%, and Calcium hydroxide nanoparticles dissolved in paraloid polymer with acetone at concentrations of 1% and 3%, in addition to nano calcium carbonate dissolved in paraloid polymer with acetone 1% and 3%. The efficiency of the consolidate materials were evaluated using a scanning electron microscope SEM, as well as measuring the water contact angle, in addition to color change testing and measuring the physical and mechanical properties.</p><h3>Conclusion</h3><p>Nano materials are considered better than paraloid B72 as a consolidated material and the best outcomes results were obtained with a nano calcium carbonate dissolved in paraloid polymer with acetone 3%.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-025-00636-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143944272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Supercritical methanolysis of leather tanning waste biomass: experimental investigation, process simulation, and sustainable biodiesel production","authors":"Moatasem Kamel,&nbsp;Leonardo Vitali,&nbsp;Ahmed Abdelnour,&nbsp;Amr A. Abdullah,&nbsp;Abo-alhassan N. Hassan","doi":"10.1186/s43088-025-00635-9","DOIUrl":"10.1186/s43088-025-00635-9","url":null,"abstract":"<div><h3>Background</h3><p>This study explores a sustainable method for biofuel production from leather tanning waste (LTW) using a novel single-step, catalyst-free supercritical methanolysis (SpCM) process. Traditional biodiesel production methods often require catalysts and involve complex purification steps, whereas this study aims to optimize process parameters to enhance biodiesel yield while reducing energy and material consumption.</p><h3>Methods</h3><p>The research employed a Box–Behnken experimental design integrated with response surface modeling (RSM) to optimize key process variables, including alcohol-to-LTW molar ratio (<i>r</i>_mo), operating temperature, pressure, and reaction duration. The produced biodiesel was characterized according to EN 14214 standards. Kinetic studies of the transesterification reaction were conducted, and a robust reactor model was developed in Aspen Plus to estimate the kinetic parameters.</p><h3>Results</h3><p>The activation energy (<i>E</i>_a) and pre-exponential factor (<i>A</i>) were determined to be 45.085 kJ/mol and 86.24 s⁻¹, respectively, with a rate constant (<i>k</i>) of 0.0098 s⁻¹ at an optimized temperature of 322.938 °C. The optimized conditions using RSM achieved a biodiesel yield of 89.35% at an <i>r</i>_mo of 32.35:1, a temperature of 322.938 °C, a pressure of 219.073 bar, and a reaction time of 14.26 min. The simulation model under the same conditions predicted a biodiesel yield of 89.49%, demonstrating excellent agreement with experimental results, with a maximum error of 0.54%.</p><h3>Conclusions</h3><p>This study presents a comprehensive approach to biodiesel production from LTW, demonstrating an efficient and sustainable alternative to conventional methods. The optimized catalyst-free SpCM process minimizes energy input and material usage while achieving high biodiesel yields. These findings contribute to waste valorization in the leather industry and support environmental sustainability efforts.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-025-00635-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143944335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis of new Cu(II), Ni(II), and Cd(II)-(N-Glycyl-L-leucine) complexes as peptide metalloantibiotics for targeting pathogenic water with antioxidant effect investigation","authors":"Safaa S. Hassan,&nbsp;Eman F. Mohamed,&nbsp;Kirolos Maged,&nbsp;Salma Hassan,&nbsp;Alaa Omran Hamad,&nbsp;Shahinda Nasr,&nbsp;Salma Reda,&nbsp;Poula Nabil,&nbsp;Andrew George,&nbsp;Mohamed M. Shoukry,&nbsp;Samar A. Aly,&nbsp;Ahmed M. Mongy,&nbsp;Entsar E. Badr,&nbsp;Khaled A. Abou Elfetouh,&nbsp;Aml M. Saleh","doi":"10.1186/s43088-025-00630-0","DOIUrl":"10.1186/s43088-025-00630-0","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Background&lt;/h3&gt;&lt;p&gt;In recent efforts to address the critical need for clean and portable water, we have focused on innovative methods to eliminate pathogenic microorganisms. To this aim, the N-Glycyl-L-leucine (&lt;b&gt;Gly-Leu)&lt;/b&gt; peptide ligand was complexed with different transition metal ions [Cu(II), Ni(II), and Cd(II)] as new peptide metalloantibiotics. The compounds were characterized and examined using various analytical methods, including elemental analysis (CHN), Fourier transform infrared spectroscopy (FTIR), assessments of magnetic properties, molar conductivity, &lt;sup&gt;1&lt;/sup&gt;HNMR, thermogravimetric analysis (TGA), and mass spectroscopy. The ligand acted as a di-anionic molecule using the carboxylate and the deprotonated amide nitrogen atom. The coordination sites were completed with carbonyl oxygen atoms and a water molecule. The complexes showed polymeric structures using bridging carboxylate groups.&lt;/p&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;p&gt;The antibacterial properties of the synthesized metal chelate were evaluated using disk diffusion and minimum inhibitory concentration methods on bacterial organisms identified from water samples taken from the Nile River. At a 1 mg/mL dose, the Cu(II)-chelate showed the biggest inhibitory zone of 27 mm against Klebsiella pneumonia, with a MIC value of 62.5 μg/mL, greater than that of the common gentamicin medication. Molecular docking investigations supported these findings, showing that Cu(II)-chelate had the lowest binding energy of − 6.16 kcal/mol, indicating significant, beneficial interactions with the amino acids in the active region of bacterial proteins. Furthermore, the Cu(II) complex and the COVID-19 main protease showed encouraging results in the docking analysis, indicating that the complex may have antiviral properties and be able to inhibit viral propagation successfully. The metal chelates demonstrated noteworthy antioxidant activity, especially against 1,1-diphenyl-2-picrylhydrazyl (DPPH radicals). The IC&lt;sub&gt;50&lt;/sub&gt; values of the antioxidant assay for Ni(II) and Cu(II) chelates were extremely similar to ascorbic acid, a common antioxidant. Their notable antioxidant capacity was demonstrated by the IC&lt;sub&gt;50&lt;/sub&gt; values of (14.4, 15.5, and 18 µg/mL) for ascorbic acid, Ni(II), and Cu(II) chelates, respectively.&lt;/p&gt;&lt;h3&gt;Conclusions&lt;/h3&gt;&lt;p&gt;Our study successfully demonstrated the potential of a new &lt;b&gt;Gly-Leu&lt;/b&gt; peptide ligand complexed with transition metal ions, particularly Cu(II), in eliminating pathogenic microorganisms from water. Cu(II)-chelate exhibited superior antibacterial properties, as confirmed by both experimental and molecular docking results. The chelates also displayed noteworthy antioxidant capacity, comparable to that of ascorbic acid. Additionally, the Cu(II)-chelate demonstrated promising antiviral potential, theoretically interacting effectively with the COVID-19 main protease, which suggests its ability to inhibit viral replication. These results underscore the potential of ","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-025-00630-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143938455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro antiproliferative activity, docking study, and plausible mode of action of (+)-2,2′-epicytoskyrin A against breast cancer MCF-7 and T47D cell lines","authors":"Agus Budiawan Naro Putra,&nbsp;Kartika Dyah Palupi,&nbsp;Ahmad Fathoni,&nbsp;Listiana Oktavia,&nbsp;Endah Puji Septisetyani,&nbsp;Arif Nurkanto,&nbsp;Praptiwi Praptiwi,&nbsp;Dewi Wulansari,&nbsp;Adi Santoso,&nbsp;Dwinna Rahmi,&nbsp;Andria Agusta","doi":"10.1186/s43088-025-00632-y","DOIUrl":"10.1186/s43088-025-00632-y","url":null,"abstract":"<div><h3>Background</h3><p>The endophytic fungus <i>Diaporthe</i> sp. GNBP-10 has been reported to produce metabolite bis-anthraquinone (+)-2,2′-epicytoskyrin A in high titers. In this study, we evaluated the potential of this metabolite as an anti-breast cancer agent by conducting antiproliferative activity studies against two breast cancer cell lines, MCF-7 and T47D.</p><h3>Materials and methods</h3><p>The antiproliferative activity of (+)-2,2′-epicytoskyrin A was determined by a 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay, fluorescence microscopy, and flow cytometry. In addition, the mode of action was assessed by several techniques, including the formation of apoptotic bodies using scanning electron microscopy (SEM) and the interaction or intercalation between (+)-2,2′-epicytoskyrin A with calf thymus DNA as indicated by the UV and ¹H-NMR spectra. Moreover, molecular docking was also conducted.</p><h3>Results</h3><p>(+)-2,2′-Epicytoskyrin A exhibited significant antiproliferative effects against MCF-7 and T47D cells. The half-maximal inhibitory concentration (IC₅₀) values for MCF-7 and T47D cells were 50.77 ± 33.23 µM and 36.24 ± 12.57 µM, respectively. (+)-2,2′-Epicytoskyrin A induced cancer cell death and promoted the formation of apoptotic bodies in both cell lines. These phenomena were expected to be facilitated by the DNA-binding ability of hydrogen atoms contained in (+)-2,2′-epicytoskyrin A. Molecular docking data indicated that (+)-2,2′-epicytoskyrin A might bind to the minor groove area of DNA, suggesting its potential as a DNA intercalator.</p><h3>Conclusion</h3><p>This study highlighted the potential of (+)-2,2′-epicytoskyrin A as a promising candidate for future breast cancer treatment.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-025-00632-y","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143930038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of the effects of Dictyota ciliolata extract on growth performance, feed utilisation, haemato-biochemical indices, hepatic antioxidants activities and immune responses of Clarias gariepinus","authors":"Ademola Z. Aderolu,&nbsp;Ismail O. Ishola,&nbsp;Uzeme P. Aluta,&nbsp;Khadijat O. Abdulwahab,&nbsp;Muyideen O. Lawal","doi":"10.1186/s43088-025-00619-9","DOIUrl":"10.1186/s43088-025-00619-9","url":null,"abstract":"<div><p>Seaweeds and their derived products represent huge resources that are still underexploited as functional ingredients in fish feeds. This study evaluated the growth performance, nutrient utilisation, antioxidation status, and immunity of <i>Clarias gariepinus</i> fed diets containing <i>Dictyota ciliolata</i> extract (DCE). Ethanolic extract of the brown seaweed <i>D</i>. <i>ciliolata</i> was obtained using the Maceration extraction method. Afterwards, four diets were prepared with DCE at inclusion levels of 0 g/kg (control), 0.25 g/kg (DCE-0.25), 0.5 g/kg (DCE-0.5) and 1.0 g/kg (DCE-1.0). One hundred and twenty fish (14.47 g/fish) were allotted to four triplicate groups (10 fish per group) and fed thrice daily with diets containing DCE at various graded levels. At the end of the feeding trial, there were no statistical differences (<i>p</i> &gt; 0.05) in final weight, weight gain and specific growth rate among the dietary group. Fish fed DCE-based diet had lower feed conversion ratio and higher protein efficiency ratio that differs significantly (<i>p</i> &lt; 0.05) from the control. Erythrocytes and leukocytes were significantly higher (<i>p</i> &lt; 0.05) in <i>C. gariepinus</i> fed DCE-0.25 and DCE-0.5 diets in comparison to the control. The liver function enzyme alkaline phosphatase was higher (<i>p</i> &lt; 0.05) in fish fed DCE-0.5 and DCE-1.0 diets than the control and DCE-0.25 groups. Dietary DCE improved antioxidant activities and decreased peroxidation of lipid in the hepatic tissue of <i>C. gariepinus</i>. The cytokines (Tumour Necrosis Factor – alpha, Interleukin-6, Interleukin-1 Beta) were significantly elevated (<i>p</i> &lt; 0.05) in the liver tissue of <i>C. gariepinus</i> fed DCE-based diets. Therefore, these results indicate that fish fed DCE diet had better nutrient utilisation, enhanced antioxidants activities and immune responses, suggesting the high suitability of <i>D. ciliolata</i> extract as a functional ingredient to improve the welfare of <i>C</i>. <i>gariepinus</i>.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-025-00619-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143930059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}