Beni-Suef University Journal of Basic and Applied Sciences最新文献

{"title":"Current trends, knowledge structure, and evidence landscape of phosphorylation-related research in organoid models: a bibliometric analysis","authors":"Rui Guo,&nbsp;Jing Jia,&nbsp;Chaojun Wei","doi":"10.1186/s43088-026-00849-5","DOIUrl":"10.1186/s43088-026-00849-5","url":null,"abstract":"<div><h3>Background</h3><p>Research on phosphorylation in organoid models has experienced rapid expansion; however, the knowledge structure, academic impact, and depth of evidence in this field remain inadequately characterized.</p><h3>Methods</h3><p>Publications indexed in the Web of Science Core Collection (SCI-EXPANDED) from 2005 to 2025 were retrieved on April 2, 2026. Following document-type restriction, deduplication, and manual screening, a total of 236 studies were included. CiteSpace was employed to analyze publication trends, collaboration patterns, co-citation structures, highly cited studies, keyword evolution, and citation-based impact. The included studies were further classified according to evidence type.</p><h3>Results</h3><p>Publication output experienced a significant increase after 2017, with the United States and China emerging as the primary contributors. However, the levels of productivity did not consistently correlate with citation impact or collaborative centrality. Co-citation analysis indicated that the field predominantly focuses on the establishment of stem cell-derived organoids, translational oncology, cerebral organoid disease models, and the signaling pathways that regulate organoid growth and differentiation. The most highly cited studies primarily centered on mechanistic phospho-signaling research, while more recent influential works have expanded into disease modeling and phosphoproteomics. Notably, 184 out of 236 studies (77.97%) were classified as providing signaling evidence, whereas only 24 studies (10.27%) offered direct evidence of phosphorylation. Furthermore, intestinal and brain organoids comprised the majority of the studies included, highlighting a pronounced tissue bias within the research.</p><h3>Conclusions</h3><p>Research on phosphorylation within organoid models is a rapidly expanding yet heterogeneous field, predominantly characterized by studies focused on signaling pathways, while direct evidence of phosphorylation remains scarce. These findings underscore significant disparities in impact, model utilization, and the depth of evidence, which should guide future mechanistic and translational research endeavors.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"15 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s43088-026-00849-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149189491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated experimental and computational evaluation of a Marsilea minuta-loaded hydrogel for enhanced wound-healing","authors":"Satyapriya Mahapatra,&nbsp;Ghanshyam Panigrahi,&nbsp;Sandesh Kumar Pattanaik,&nbsp;Lorie Dehury,&nbsp;Ashutosh Prasad Ghadei,&nbsp;Diptirani Rath","doi":"10.1186/s43088-026-00845-9","DOIUrl":"10.1186/s43088-026-00845-9","url":null,"abstract":"<div><h3>Background</h3><p><i>Marsilea minuta</i> L., a medicinal aquatic fern is of ethnopharmacological importance as per various traditional systems of medicine. It is used to treat skin disorders like wound in India.This study aims to explore the effect of the methanolic extract <i>of M. minuta</i> by using both in silico and in-vivo techniques in the treatment of wounds.</p><h3>Methods</h3><p>The plant material <i>Marsilea minuta</i> L., (MEMM) was defatted first with petroleum ether to remove the fat and then extracted with solvent methanol in a Soxhlet apparatus. GC-MS analysis was used to identify the compound annotations tentatively in the methanolic extract. The methanolic extract was prepared into a hydrogel for the investigation of wound-healing activity using the excision model in rats. The animals were divided into four groups i.e. a standard group (STD) that treated with Silver nitrate gel, a normal control group (NC) treated with Gel base, and two groups treated with the MEMM-loaded hydrogel formulations (MEMM 5 % w/v and MEMM 10 % w/v), containing six animals per group. The primary end point was wound contraction area, and additionally % of wound-healing and histopathological examination were adopted. Additionally, in-silico molecular docking was conducted against the target proteins to support our findings. One-way ANOVA was used to examine group differences, followed by Tukey's post hoc test for multiple comparisions.</p><h3>Results</h3><p>The experimental evidence shows that the 10 % w/v hydrogel group exhibits effective wound-healing activity as compared to that of the standard group. Histopathological examination confirms collagen formations, which accelerated re-epithelialization and remodelling of the wound. Further, the in-silico investigations support our findings, as the tentatively identified compounds showed moderate to strong binding affinities for wound-healing targets such as VEGFR-2, COX-2, and MMP-9.</p><h3>Conclusion</h3><p>This study concludes that <i>M. minuta</i> contains a diverse array of tentatively identified compounds that support the plant’s wound-healing activity. Overall, this investigation of scientific evidence supports the ethnopharmacological claim and highlights it as a promising, adjunctive natural therapeutic candidate for wound-healing.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"15 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s43088-026-00845-9.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149132429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The efficacy of the crude Origanum genus (Lamiaceae) essential oils on experimentally infected mice with Blastocystis spp.","authors":"Ekhlas H. Abdel-Hafeez,&nbsp;Mohamed M. Abdel-Fatah,&nbsp;Manar M. Sanadeki,&nbsp;Manar M. S. A. Nasr,&nbsp;Shaimaa M. Hafez,&nbsp;Reham A. M. Abd Rabou","doi":"10.1186/s43088-026-00838-8","DOIUrl":"10.1186/s43088-026-00838-8","url":null,"abstract":"<div><h3>Background</h3><p><i>Blastocystis</i> spp. is a common intestinal protozoan with a controversial role in gastrointestinal disease, and the variable efficacy of conventional therapies has prompted the search for alternative treatment options. This study evaluated the therapeutic effects of crude <i>Origanum</i> essential oil in experimentally infected mice compared with nitazoxanide (NTZ).</p><h3>Methods</h3><p>Forty male Swiss albino mice were allocated into four groups: negative control, infected untreated, NTZ-treated (<span>(:100text{:mg/kg/day})</span>), and crude <i>Origanum</i> oil-treated (<span>(:0.5text{:mL/kg/day})</span>). The essential oil was evaluated in its crude form without chemical characterization (e.g., GC-MS). Mice were orally infected with <span>(:1times:{10}^{5}text{:Blastocystis})</span> cysts and treated for seven days. Treatment efficacy was assessed by fecal cyst counts, intestinal nitric oxide (NO) and malondialdehyde (MDA) levels, and histopathological examination of intestinal tissues. Data was analyzed using one-way ANOVA.</p><h3>Results</h3><p>Infected untreated mice exhibited reduced activity and soft stools. Clinical improvement was observed in both treatment groups within 5–7 days, and no mortality occurred during the study. The infected untreated group showed the highest cyst burden (<span>(:6.2times:{10}^{5}pm:0.4times:{10}^{5}text{:cysts/g:stool})</span>), whereas NTZ and crude <i>Origanum</i> oil treatment significantly reduced cyst shedding by <span>(:85.6%)</span> and <span>(:91.3%)</span>, respectively, compared with infected controls (<span>(:p&lt;0.001)</span>). No cysts were detected in the non-infected control group. Infection was associated with significant increases in intestinal NO and MDA levels, indicating oxidative stress and inflammation. Both treatments significantly reduced these markers (<span>(:p&lt;0.001)</span>), with values in the <i>Origanum</i>-treated group approaching those of the negative control group. Histopathological examination revealed villous atrophy, epithelial desquamation, and inflammatory cell infiltration in infected untreated mice. NTZ treatment resulted in partial improvement of these lesions, whereas crude <i>Origanum</i> oil treatment was associated with marked restoration of villous architecture and minimal inflammatory changes.</p><h3>Conclusions</h3><p>Under this experimental model, crude <i>Origanum vulgare</i> essential oil improved parasitological, biochemical, and histopathological parameters in <i>Blastocystis</i>-infected mice. Because chemical profiling was not performed, these effects reflect the biological activity of the crude extract as a whole. Further studies incorporating GC-MS characterization are needed to identify active constituents, optimize dosing, and confirm these findings in other models and clinical settings.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"15 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s43088-026-00838-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149132311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Computational elucidation of the potential mechanisms of Warburgia ugandensis against colorectal cancer","authors":"Yohana Amos,&nbsp;Bienvenu Nsengimaana,&nbsp;Mercy Bisola Faleyimu,&nbsp;Peter Olorundunsin Adeniyi,&nbsp;Aishat Adebola Lawal,&nbsp;Orace Mathieu Kenou,&nbsp;Darius Riziki Martin,&nbsp;Ashita Jawali,&nbsp;Olaitan I. Awe","doi":"10.1186/s43088-026-00840-0","DOIUrl":"10.1186/s43088-026-00840-0","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Background&lt;/h3&gt;&lt;p&gt;Colorectal cancer remains a major global health challenge due to its high incidence, mortality, therapeutic resistance, and treatment-related adverse effects. &lt;i&gt;Warburgia ugandensis&lt;/i&gt; is a medicinal plant that has demonstrated anti-colorectal cancer; however, the specific bioactive compounds responsible and their underlying molecular mechanisms remain underexplored. This study investigated the anti-colorectal cancer potential and molecular mechanisms of &lt;i&gt;W. ugandensis&lt;/i&gt; phytochemicals using an integrated computational approach.&lt;/p&gt;&lt;h3&gt;Methods&lt;/h3&gt;&lt;p&gt;Phytochemicals were retrieved from published literature and the Collection of Open Natural Products database, standardized, and screened for drug-likeness and oral bioavailability. Potential protein targets were predicted and intersected with colorectal cancer-associated genes, followed by network pharmacology and functional enrichment analyses. Molecular docking, 100 ns molecular dynamics simulations, molecular mechanics Poisson-Boltzmann surface area binding energy calculations, and absorption, distribution, metabolism, excretion, and toxicity (ADMET) analyses were performed to evaluate compound-target interactions, complex stability, and pharmacological properties.&lt;/p&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;p&gt;Among 242 identified phytochemicals, 21 compounds satisfied drug-likeness and oral bioavailability criteria. Target prediction identified 435 potential protein targets of the screened phytochemicals, of which 411 overlapped with colorectal cancer-associated genes. Network analysis of the overlapping targets identified PIK3CA, PIK3CD, GNAI1, GNAI3, and SRC as key hub genes. Functional enrichment analysis suggested that these targets are involved in cancer-relevant signaling pathways, including the phosphatidylinositol 3-kinase-protein kinase B, insulin receptor, and relaxin signaling pathways. Molecular docking identified quercetin, kaempferol, and bemadienolide as leading candidates, with strong binding affinities toward GNAI1 (-9.8 kcal/mol), GNAI3 (-9.6 kcal/mol), and PIK3CD (-8.7 kcal/mol), respectively. Molecular dynamics simulations demonstrated stable protein-ligand complexes with reduced structural fluctuations over 100 ns. Binding free energy analysis revealed favorable interactions, with quercetin-GNAI1 showing the strongest predicted binding stability (-86.2 ± 3.3 kcal/mol). Pharmacokinetic and toxicity predictions indicated high gastrointestinal absorption and generally low predicted toxicity profiles.&lt;/p&gt;&lt;h3&gt;Conclusion&lt;/h3&gt;&lt;p&gt;This study provides computational, hypothesis-generating evidence that &lt;i&gt;Warburgia ugandensis&lt;/i&gt; phytochemicals may modulate multiple molecular targets and signaling pathways involved in colorectal cancer. Quercetin, kaempferol, and bemadienolide represent promising candidates for further experimental investigation. Nevertheless, these predictive findings require validation through in-vitro and in-vivo studies to establish their therapeutic releva","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"15 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s43088-026-00840-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149132243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In silico identification and design of 2-(3-methoxyphenoxy)-N-methylacetamide analogs as anti-tuberculosis transcriptional regulator (EthR) inhibitors via E-pharmacophore modeling, QSAR, docking, MMGBSA, ADMET, and molecular dynamics","authors":"Anne Jibrin,&nbsp;Adamu Uzairu,&nbsp;Imran Mohd,&nbsp;Gideon Adamu Shallangwa,&nbsp;Stephen Eyije Abechi,&nbsp;Abdullahi Bello Umar","doi":"10.1186/s43088-026-00827-x","DOIUrl":"10.1186/s43088-026-00827-x","url":null,"abstract":"<div><h3>Background</h3><p>Tuberculosis (TB) remains a leading cause of infectious mortality worldwide, exacerbated by the emergence of multidrug-resistant strains and the dose-limiting toxicity of existing chemotherapeutics. Targeting regulatory mechanisms that potentiate current drugs offers a promising alternative strategy. EthR, a TetR-family transcriptional repressor that downregulates <i>ethA</i> and thereby limits ethionamide activation, has emerged as an attractive molecular target. Inhibiting EthR can enhance ethionamide efficacy while potentially reducing required dosage. Although 2-(3-methoxyphenoxy)-<i>N</i>-methylacetamide derivatives have been reported as EthR binders, their pharmacophoric features, structure–activity relationships, and dynamic interaction profiles remain insufficiently characterized.</p><h3>Methods</h3><p>In this study, a comprehensive in silico workflow was employed to design and evaluate novel 2-(3-methoxyphenoxy)-<i>N</i>-methylacetamide analogs as EthR inhibitors. An energetically optimized e-pharmacophore model was developed and used to virtually screen a curated compound library. Quantitative structure–activity relationship (QSAR) modeling was performed to elucidate key molecular descriptors governing inhibitory activity. High-ranking compounds were subjected to molecular docking, MM-GBSA binding free-energy calculations, ADMET prediction, and 100 ns molecular dynamics (MD) simulations to assess binding stability and drug-likeness.</p><h3>Results</h3><p>The generated e-pharmacophore hypothesis effectively captured essential steric and electronic features required for EthR inhibition. The QSAR model demonstrated robust predictive performance (R² = 0.8289; Q² = 0.6383), revealing a strong correlation between functional-group distribution and bioactivity. Docking studies identified several analogs with high affinity for the hydrophobic regulatory pocket of EthR, stabilized by persistent hydrogen-bond interactions with key residues including Trp103, Asn179, and Phe184. MMGBSA analysis confirmed favorable binding free energies ranging from − 71.43 to − 96.28 kcal/mol. The selected hits exhibited acceptable pharmacokinetic properties and low predicted toxicity. MD simulations further demonstrated conformational stability of the protein-ligand complexes, with low RMSD fluctuations and sustained intermolecular interactions throughout the simulation period.</p><h3>Conclusions</h3><p>This integrative computational investigation identifies 2-(3-methoxyphenoxy)-<i>N</i>-methylacetamide analogs as promising EthR inhibitors with favorable binding energetics, stability, and drug-like characteristics. By elucidating their pharmacophoric requirements and dynamic binding behavior, the study provides a mechanistically informed framework for the rational development of EthR-targeted adjunct therapies, offering a viable pathway toward improved anti-tuberculosis treatment strategies.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"15 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s43088-026-00827-x.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149132244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Existence and Hyers-Ulam stability of a coupled fractional differential system involving the ({rm{Psi }})-Caputo derivative and mixed boundary conditions","authors":"Ouarda Saifia,&nbsp;Adel Lachouri,&nbsp;Mohammad Esmael Samei","doi":"10.1186/s43088-026-00828-w","DOIUrl":"10.1186/s43088-026-00828-w","url":null,"abstract":"<div><p>In this study, the Banach contraction principle and Schaefer’s fixed point theorem are employed to establish, respectively, the uniqueness and existence of solutions for a coupled fractional differential system subject to mixed boundary conditions. These conditions consist of Dirichlet conditions and nonlocal fractional integral conditions involving the <span>(Psi)</span>-Riemann-Liouville fractional integral evaluated at the interior point <span>(gamma)</span>. Furthermore, the Hyers-Ulam stability of the proposed system is examined. Finally, an illustrative example is provided to support the theoretical findings.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"15 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s43088-026-00828-w.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149133335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lycopene partially ameliorates alcohol-induced sperm dysfunction and testicular toxicity in male Wistar rats","authors":"Oore-oluwapo Ololade Daramola,&nbsp;Adekunle Wahab Oyeyemi,&nbsp;Adeniran O. Akinola,&nbsp;Miracle Deborah Adeniyi,&nbsp;Ayomikun Joyce Tomomewo,&nbsp;Divine Ndako,&nbsp;Jesutofunmi Oguntimehin,&nbsp;Babatunde Oguntimehin,&nbsp;Dayo Rotimi Omotoso","doi":"10.1186/s43088-026-00843-x","DOIUrl":"10.1186/s43088-026-00843-x","url":null,"abstract":"<div><h3>Background</h3><p>Alcohol consumption predisposes one to various hazards like reproductive toxicity. Lycopene, a carotenoid with antioxidant capacity credited with the ability to decrease oxidative stress has been reportedly used to ameliorate reproductive damage in experimental animals. This research investigated the mitigating abilities of lycopene administration on reproductive toxicity caused by alcohol in male Wistar rats.</p><h3>Methods</h3><p>Twenty male Wistar rats (150–180 g) were grouped as follows: 1 (control; 3 mL/kg 1% tween 80), 2 (Alcohol; Alc 3.2 g/kg of 30% v/v), 3 (lycopene; Lyc10 mg/kg) and 4 (Alc + Lyc). They were orally treated for 35 days and anaesthetized on day 36. Spermatozoa were profiled microscopically. 8-hydroxydeoxyguanosine, Caspase-3 activities, <i>BcL-2</i> and interleukin-1β (IL-1β) were evaluated using Enzyme Linked Immunosorbent Assay (ELISA) technique. Malondialdehyde (MDA) concentrations, superoxide dismutase (SOD) and catalase were spectrophotometrically determined and testicular histopathology was assessed. Data derived was statistically analysed, expressed as Mean ± standard error of mean (Mean ± SEM) and compared using one way analysis of variance followed by Waller-Duncan’s post-hoc test.</p><h3>Result</h3><p>Alcohol caused an increase in abnormal morphology of spermatozoa. It also caused an increase in MDA concentration, Caspase-3 and IL-1β expression while a decrease in SOD, catalase activities and zinc level were observed. In addition, alcohol exposure caused histopathological changes within the testicular tissue. However, lycopene administration ameliorated all the observed deleterious changes induced by alcohol exposure.</p><h3>Conclusion</h3><p>Lycopene improved sperm morphology, testicular catalase activity and zinc level, lowered MDA level, Caspase 3 and interleukin 1 expression and reversed testicular histopathology in rats subjected to damages caused by alcohol.</p><h3>Clinical trial number</h3><p>Not applicable.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"15 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s43088-026-00843-x.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149133137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineered bimetallic Ag-Se nanozyme with multifunctional biomedical potential","authors":"Rasitha Arafa Rafiudeen,&nbsp;S. Ranjani,&nbsp;S. Hemalatha","doi":"10.1186/s43088-026-00841-z","DOIUrl":"10.1186/s43088-026-00841-z","url":null,"abstract":"<div><h3>Background</h3><p>Multidrug-resistant Gram-negative ESBL-producers including <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> are becoming a critical obstacle in the treatment of chronic diabetic wounds. Biogenesis of nanoparticles with plant phytochemicals is a strong alternative antimicrobial treatment. <i>Tylophora indica</i>, a medicinal plant native to South Asia and widely used in traditional medicine, has been reported to exhibit anticancer and antimicrobial activities.</p><h3>Methods</h3><p>This study focuses on green synthesis of silver-selenium nanoparticles called bimetallic Ag-Se nanozyme (TiAg-SeNZ) with <i>T. indica</i> aqueous extract. This valorized approach not only utilizes plant materials effectively, but also contributes to development of sustainable nanomaterial.</p><h3>Results</h3><p>Synthesized TiAg-SeNZ was characterized by UV-Vis spectroscopy, FTIR, XRD, DLS and zeta potential analyses. FESEM coupled with EDAX analyses of TiAg-SeNZ revealed a porous, quasi-spherical aggregated morphology with a silver-rich, AgCl-dominant bimetallic structure, with selenium incorporated in amorphous form within the phytochemical-rich organic matrix. Nanotoxicity assessment in <i>Danio rerio</i> (zebrafish) embryo demonstrated biocompatibility at ≤ 25 µg/ml. TiAg-SeNZ showed multipotent ability including anti-diabetic, anti-inflammatory, antioxidant, and strong antimicrobial activities in pathogenic <i>E. coli</i> and <i>K. pneumoniae</i> with MIC and MBC values ranging between 3 and 25 µg/ml and 6–50 µg/ml, respectively. Anti-biofilm activity reached up to approximately 85% at MIC concentration. These findings highlight the therapeutic potential of <i>T. indica</i> derived TiAg-SeNZ for topical applications against ESBL-producing bacteria associated with diabetic wound infection. However, further biocompatibility and wound-healing studies are required to validate its therapeutic potential.</p><h3>Conclusion</h3><p>These findings demonstrate the potential of sustainably engineered plant-based bimetallic Ag-Se nanozyme as functional nanomaterials for advanced biomedical applications. The study paves the way for the development of eco-conscious topical therapeutics for diabetic wound infections, aligning with global efforts in the development of sustainable healthcare materials.</p><h3>Clinical trial number</h3><p>Not applicable</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"15 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s43088-026-00841-z.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149034970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eco-pharmacovigilance in traditional Chinese medicine: a review of mechanisms, challenges, and a call for a “One Health” approach","authors":"Wenhao Zhong,&nbsp;Wenren Zhao,&nbsp;Xiaoxiao Cai,&nbsp;Ruoyu Gan,&nbsp;Yan Lin,&nbsp;Yu Wang,&nbsp;Xiaomeng Zhang,&nbsp;Bing Zhang,&nbsp;Zhijian Lin","doi":"10.1186/s43088-026-00836-w","DOIUrl":"10.1186/s43088-026-00836-w","url":null,"abstract":"<div><p>Eco-pharmacovigilance (EPV) conventionally addresses the environmental impacts of pharmaceuticals. However, extending EPV to Traditional Chinese Medicine (TCM) necessitates evaluating a product life cycle that is deeply intertwined with natural ecosystems. This review investigates the environmental footprint of TCM through a ‘One Health’ lens. We systematically evaluated contamination pathways, including heavy metal accumulation in medicinal plants and pollutant mobilization facilitated by dissolved organic matter from extraction residues. Furthermore, we analyzed under-investigated risks, such as the environmental dissemination of antibiotic resistance genes, the endocrine-disrupting properties of specific herbal compounds, and the implications of climate change for the quality and safety of medicinal plants. To compile this evidence, a systematic literature search was conducted across multiple academic databases, primarily PubMed, Web of Science, Scopus, and the China National Knowledge Infrastructure (CNKI), covering publications up to May 2026. The search strategy utilized terms including ‘eco-pharmacovigilance’, ‘traditional Chinese medicine’, ‘One Health’, ‘heavy metals’, ‘medicinal plants’, ‘antibiotic resistance genes’, ‘endocrine-disrupting compounds’, ‘life cycle assessment’, ‘dissolved organic matter’, and ‘green manufacturing’, both individually and in combination. Following a rigorous screening process for relevance, methodological quality, and thematic scope, a total of 62 peer-reviewed references were included in this comprehensive analysis. Based on the synthesized data, this review identifies critical gaps in current EPV frameworks and proposes an integrated strategy combining mechanistic toxicology, climate resilience planning, green manufacturing technologies, and international regulatory cooperation to advance the ecological sustainability of TCM.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"15 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s43088-026-00836-w.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148927094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A prospective observational pre-post study of dental anxiety associated with rubber dam isolation in patients undergoing restorative procedures","authors":"Mirnalini Selvakumar,&nbsp;Madhumitha Raju,&nbsp;Mahalakshmi Srinivasan","doi":"10.1186/s43088-026-00831-1","DOIUrl":"10.1186/s43088-026-00831-1","url":null,"abstract":"<div><h3>Aim</h3><p>To assess pre-treatment and post-treatment dental anxiety levels in patients undergoing restorative procedures using rubber dams.</p><h3>Methods</h3><p>A prospective observational pre-post study was conducted on 102 patients treated at the Department of Conservative Dentistry and Endodontics of a reputed private Dental College. Informed consent was obtained from all the participants. A pre-treatment questionnaire assessing baseline anxiety (MDAS) was administered before the procedure. The patients were then educated about the rubber dam kit and its clinical significance. At the end of the dental procedure, a post-treatment questionnaire was administered. Statistical analysis was performed using the Statistical Package for the Social Sciences (SPSS) version 25.0, with a significance level set at p &lt; 0.05. Descriptive statistics, mean calculation, Chi square test and Linear Regression analysis was performed.</p><h3>Results</h3><p>A total of 102 participants were included, comprising 52.0% males and 48.0% females. The mean pre-treatment MDAS score was 9.84 ± 4.54. Overall, 53.9% of participants demonstrated low anxiety, 40.2% moderate anxiety, and 5.9% high anxiety. Female participants reported significantly greater tenderness-related anxiety during rubber dam placement and removal. Occupation showed significant associations with anxiety measures (p &lt; 0.05), with unemployed participants exhibiting higher anxiety than students and employed individuals. Multiple linear regression identified occupation as a significant predictor of dental anxiety (β = 0.250, p = 0.006), while age and gender were not significant predictors. Verbal explanation of the rubber dam procedure improved treatment acceptance and reduced anxiety among participants</p><h3>Conclusion</h3><p>Within the limitations of the study, factors such as occupation influenced anxiety levels after rubber dam usage, and the results were statistically significant. Pressure or discomfort felt on the gums during the placement and removal of the rubber dam increases anxiety in patients. A verbal explanation of the rubber dam prior to treatment had a positive effect on treatment acceptance and reduced anxiety.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"15 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s43088-026-00831-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148927095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}