Beni-Suef University Journal of Basic and Applied Sciences最新文献

{"title":"Data-driven optimization of friction stir welding parameters of AA7075 aluminium alloy using ANN surrogates and genetic algorithms","authors":"Omnia Abouhabaga,&nbsp;Eman El Shrief","doi":"10.1186/s43088-026-00755-w","DOIUrl":"10.1186/s43088-026-00755-w","url":null,"abstract":"<div><h3>Background</h3><p>This study proposes a data-driven hybrid framework for optimizing the process parameters of friction stir welding (FSW) of AA7075 aluminium alloy by integrating artificial neural network (ANN) surrogate models with genetic algorithm (GA) optimization<b>.</b></p><h3>Methods</h3><p>A Design of Experiments (DoE) approach comprising 27 systematically designed trials was employed to investigate the combined influence of rotational speed, welding speed, plunge depth, and shoulder-to-pin diameter ratio on the ultimate tensile strength (UTS) and microhardness of FSW joints. The experimental data were used to train and validate ANN surrogate models capable of capturing the nonlinear and interdependent relationships between the process variables and mechanical responses.</p><h3>Results</h3><p>The developed ANN models demonstrated high predictive accuracy, with R2 values exceeding 0.94 and 0.96 for ultimate tensile strength (UTS) and hardness, respectively, accompanied by low root mean square error (RMSE) and mean absolute error (MAE) values. Experimental validation confirmed the robustness of the models, yielding prediction errors ranging from 0.18% to 11%, with an average deviation of 3.6%. Coupling the trained ANN surrogate with a GA-based optimizer enabled the identification of parameter combinations that simultaneously maximize UTS and hardness. The optimal UTS of 345.76 MPa was obtained at a rotational speed of 814.13 rpm, welding speed of 51.53 mm/min, plunge depth of 0.213 mm, and a shoulder-to-pin ratio of 3.6, whereas the maximum hardness of 143.41 HV was achieved at lower rotational and welding speeds, combined with a higher plunge depth and shoulder-to-pin ratio.</p><h3>Conclusion</h3><p>The findings demonstrate that the ANN–GA hybrid approach provides a robust, accurate, and computationally efficient tool for predictive modelling and process optimization in FSW. This framework offers valuable potential for Industry 4.0 and intelligent manufacturing applications, particularly in the design of lightweight, high-performance aluminium structures.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"15 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s43088-026-00755-w.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147829387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Remediation of poultry wastewater contaminated with multi-drug-resistant Pseudomonas aeruginosa using Triazole-modified MIL-53(Fe)","authors":"Sahar A. Abdel Aziz,&nbsp;Maha B. Abd Elhaleem,&nbsp;Safaa M. Abbas,&nbsp;Sawsan Abd Elkhalik,&nbsp;Fatma M. Elantabli","doi":"10.1186/s43088-026-00745-y","DOIUrl":"10.1186/s43088-026-00745-y","url":null,"abstract":"<div><h3>Background</h3><p>Multi-drug-resistant spectrum of <i>Pseudomonas aeruginosa</i> (<i>P. aeruginosa</i>) is frequently issue in wastewater of poultry industry, posing a major public hazard. Also, the incorporation of promising nano-scale materials, notably metal organic frameworks (MOFs), is an auspicious tool for enhancing the biological quality of wastewater.</p><h3>Methods</h3><p>A total of wastewater samples (<i>n</i> = 50) were collected to from randomly chosen poultry shops in Beni-Suef Governorate, Egypt, to isolate <i>P. aeruginosa</i>, the antibiotic profile of the most frequently used antibiotics in the poultry farming sector was investigated, as well as the dissemination of antibiotic coding genes (<i>blaTEM</i> and <i>blaKPC</i> genes) through the molecular techniques was done, and finally, antimicrobial evaluation of the synthesized 3-amino-1,2,4-triazole, MOF, and T@MOFs composite is carried out against strains of <i>P. aeruginosa</i> exhibiting MRD.</p><h3>Results</h3><p><i>P. aeruginosa</i> identified in 68.0% of wastewater samples, showing multi-drug resistance to most of antibiotics including nalidixic acid, ciprofloxacin, amikacin cefoxitin, clindamycin, lincomycin, and gentamicin, with a Multiple Antibiotic Resistance (MAR) index extending from 0.5 to 0.83. Additionally, T@MOFs composite demonstrated a significant bactericidal potency indicated by a minimum bactericidal concentration of 83.33 ± 2.83 μg/mL, a minimum inhibitory concentration of 41.63 ± 10.43 μg/mL as well as a prominent inhibition zone diameter that measured 20.00 ± 2.31 mm.</p><h3>Conclusion</h3><p>The study reveals that wastewater in the examined areas poses noteworthy reservoir for the spread of MDR <i>P. aeruginosa</i> and their encoding genes. Also, T@MOFs composite displays exceptional antimicrobial efficacy against <i>P. aeruginosa</i> isolates, with a promising issue for critical wastewater bioremediation, addressing worldwide water scarcity challenges.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"15 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s43088-026-00745-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147796959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring new solutions for the stochastic Gardner equation forced by advection Brownian motion used in nonlinear wave propagation","authors":"Sofian T. Obeidat,&nbsp;Hamdy Ahmed,&nbsp;Mohamed S. Algolam,&nbsp;Hijyah M. Alshammary,&nbsp;Wael W. Mohammed","doi":"10.1186/s43088-026-00761-y","DOIUrl":"10.1186/s43088-026-00761-y","url":null,"abstract":"<div><h3>Background</h3><p>The stochastic Gardner equation with Itô-type advection Brownian motion provides an effective mathematical framework for modeling the propagation of nonlinear waves in random environments. It plays a key role in understanding the transition from coherent wave structures to irregular dynamics by elucidating the interplay among randomness, nonlinearity, and dispersion in complex physical systems. Consequently, obtaining exact analytical solutions of the stochastic Gardner equation is of significant theoretical and practical importance.</p><h3>Methods</h3><p>By employing appropriate transformation techniques together with Itô calculus, the stochastic Gardner equation is decomposed into two coupled components: a deterministic Gardner equation with an additional diffusion term and a stochastic ordinary differential equation. The extended tanh-function method is applied to derive exact traveling wave solutions of the deterministic Gardner equation. These solutions are then combined with the analytical solution of the stochastic ordinary differential equation to construct exact solutions of the original stochastic Gardner equation. The effectiveness of the proposed framework can be demonstrated by deriving various stochastic wave solutions and graphing them, thus proving its ability to investigate nonlinear wave propagation in the presence of stochastic effects.</p><h3>Results</h3><p>A variety of exact analytical solutions for the stochastic Gardner equation are successfully obtained, including solitary wave structures influenced by stochastic effects. The impact of advection Brownian motion on the wave dynamics is systematically investigated. Three-dimensional graphical simulations, generated using MATLAB, illustrate how stochastic advection modifies the shape, amplitude, and evolution of the solutions compared to their deterministic counterparts. Additionally, these findings shed light on how stochastic perturbations affect the amplitude and propagation characteristics of nonlinear waves in the Gardner model.</p><h3>Conclusion</h3><p>The proposed analytical framework provides explicit exact solutions for the stochastic Gardner equation and reveals the significant role of advection Brownian motion in altering nonlinear wave behavior. These results enhance the understanding of stochastic nonlinear wave propagation and may be useful for modeling realistic physical systems subject to random perturbations.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"15 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s43088-026-00761-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147738476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Existence and uniqueness results for mixed pantograph fractional differential equations equipped with the p-Laplacian operator with impulsive boundary conditions","authors":"Elham Yousefi,&nbsp;Mozhgan Akbari,&nbsp;Mohammad Esmael Samei","doi":"10.1186/s43088-026-00748-9","DOIUrl":"10.1186/s43088-026-00748-9","url":null,"abstract":"<div><p>Mixed fractional boundary value problems with impulsive conditions have attracted significant attention due to their applications in various fields. In this study, we investigate the existence and uniqueness of solutions for a mixed fractional boundary value problem with impulsive conditions involving the <span>(texttt{p})</span>-Laplacian operator. The analysis is conducted using the Leray–Schauder and Banach fixed point theorems. Our theoretical findings confirm the existence and uniqueness of solutions under suitable assumptions. To illustrate the applicability of our results, two examples are provided at the end of the paper. The results contribute to the theory of fractional differential equations with impulsive effects and pave the way for further research on related nonlinear boundary value problems.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"15 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s43088-026-00748-9.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147738289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decoding precision medicine in prediabetes: a citespace-based bibliometric analysis of current trends and future directions","authors":"Manru Xu,&nbsp;Hanyue Gan,&nbsp;Furong Zhong,&nbsp;Mengyuan Qiao,&nbsp;Pan Ren,&nbsp;Yue Zhu,&nbsp;Qi Wang,&nbsp;Wenbin Wu","doi":"10.1186/s43088-026-00762-x","DOIUrl":"10.1186/s43088-026-00762-x","url":null,"abstract":"<div><h3>Background</h3><p>Prediabetes is characterized by its high global prevalence and significant risk of progression to diabetes. The current research focus lies in precise risk stratification and intervention, wherein precision medicine plays a critical role by integrating multi-omics data with clinical information. This study examines how precision medicine concepts, including risk stratification, biomarker-guided subtyping, and individualized intervention, have progressively permeated prediabetes research, acknowledging that these ideas often integrate with, rather than replace, traditional prevention frameworks.</p><h3>Methods</h3><p>Publications were retrieved from the Web of Science Core Collection (WoSCC). Visualization and quantitative analyses were conducted using CiteSpace 6.4.R1 and VOSviewer 1.6.20.</p><h3>Result</h3><p>A total of 103 publications were included, involving 607 authors from 347 institutions across 50 countries/regions. The USA led in publication volume and international collaboration, with Harvard Medical School and the University of Copenhagen emerging as the most influential institutions. Journals with high impact factors, such as The Lancet Diabetes and Endocrinology and The New England Journal of Medicine, accounted for a substantial share of citations in this corpus. Keyword co-occurrence and burst analyses revealed that research hotspots have shifted from metabolic risk factors such as hypertension and impaired glucose tolerance toward insulin resistance, genetic biomarkers, precision nutrition, and artificial intelligence–assisted patient stratification.</p><h3>Conclusions</h3><p>Current research emphasizes individualized intervention strategies supported by multi-omics technologies, continuous glucose monitoring, and artificial intelligence. Future efforts should focus on integrating dynamic biomarkers and personalized nutrition into scalable precision prevention frameworks, offering new avenues for early diagnosis, timely treatment, and stratified interventions in prediabetes.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"15 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s43088-026-00762-x.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147737832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical audit of testosterone therapy practice patterns for hypoactive sexual desire dysfunction in postmenopausal women","authors":"Bhawna Sharma,&nbsp;Shiaam Thava,&nbsp;Mena Abdalla","doi":"10.1186/s43088-026-00766-7","DOIUrl":"10.1186/s43088-026-00766-7","url":null,"abstract":"<div><h3>Background</h3><p>This retrospective clinical audit evaluates practice patterns and associative outcomes of testosterone therapy for hypoactive sexual desire dysfunction (HSDD) in postmenopausal women. A clinical audit was conducted on 45 postmenopausal women attending a postmenopausal clinic between July 1, 2024, and December 31, 2024, followed for a 3-month period.</p><h3>Methods</h3><p>Data extracted included presenting complaints, HSDD symptom identification, baseline and follow-up testosterone levels, counseling on side effects, and adherence to standard dosing protocols. Simulated data, based on observed audit criteria adherence rates from clinical practice, were utilized to demonstrate analytical methodology. Missing data (35.6%) were handled using complete case analysis after confirming missingness completely at random (MCAR).</p><h3>Results</h3><p>Results suggest an association between testosterone therapy and increased testosterone levels from baseline (17.7 ± 4.8 ng/dL) to 3-month follow-up (39.5 ± 7.6 ng/dL) in patients with complete paired data (n = 29) (mean difference: 21.8 ng/dL, 95% CI: [18.2, 25.3], <i>p</i> &lt; 0.001, Cohen’s d: 2.33). Patient compliance rate was 91.1% (95% CI: [79.3%, 96.5%]). Clinical improvement in HSDD symptoms, defined a priori as an increase of ≥ 1.2 points on the Female Sexual Function Index (FSFI) desire domain, was reported in 78% of patients (95% CI: [63.7%, 87.5%]). While the audit criteria for HSDD screening and appropriate prescribing showed high adherence (95.6% and 100% respectively), there were areas for improvement in baseline (68.9%) and follow-up (64.4%) testosterone level measurements.</p><h3>Conclusion</h3><p>This clinical audit highlights the associative benefits of testosterone therapy for HSDD and underscores the critical importance of adherence to clinical guidelines for monitoring. Testosterone therapy is associated with increased circulating testosterone levels in postmenopausal women with corresponding clinical symptom improvement. However, given the use of simulated data for analytical demonstration, definitive conclusions regarding treatment effectiveness cannot be drawn. Further prospective studies with larger cohorts and high-quality randomized controlled trials are warranted to confirm these findings.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"15 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s43088-026-00766-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147737833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"De novo design and evaluation of dual inhibitory peptide binders targeting MDMX-p53 and MDM2-p53 interactions","authors":"Olanrewaju Ayodeji Durojaye,&nbsp;Sm Faysal Bellah,&nbsp;Henrietta Uzoeto,&nbsp;Somtochukwu Ezechukwu,&nbsp;Amarachukwu Arazu,&nbsp;Judith Nnedimkpa Ajima,&nbsp;Anthonia Ngozi Ngwu,&nbsp;Samuel Cosmas","doi":"10.1186/s43088-026-00765-8","DOIUrl":"10.1186/s43088-026-00765-8","url":null,"abstract":"<div><h3>Background</h3><p>The MDMX-p53 and MDM2-p53 interactions are critical regulators of the tumor-suppressive functions of p53, often disrupted in cancers through overexpression of murine double minute X (MDMX) and murine double minute 2 (MDM2).</p><h3>Methods</h3><p>In this study, we designed and computationally evaluated dual inhibitory peptides using RFdiffusion, ProteinMPNN, AlphaFold Multimer, and AlphaFold 3. Electrostatic complementarity, thermal stability, and binding affinity were assessed, followed by 300 ns molecular dynamics (MD) simulations.</p><h3>Results</h3><p>Mb2 and Mb4 (MDMX/2 binder 2 and 4) exhibited improved predicted binding affinity, enhanced electrostatic complementarity, and higher thermal stability relative to p53. Structural modeling and comparative validation confirmed reliable peptide–protein interactions. MD simulations further demonstrated stable trajectories, reduced conformational fluctuations, and persistent binding of Mb2 and Mb4 to both MDMX and MDM2.</p><h3>Conclusions</h3><p>These findings identify Mb2 and Mb4 as promising dual inhibitory peptides with potential for restoring p53 activity. This study provides a computational foundation for future experimental validation and therapeutic development.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"15 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s43088-026-00765-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147737971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization and bioactivities of a novel copper–zinc superoxide dismutase from the marine snail Rapana venosa: antioxidant, antimicrobial, and anticancer effects","authors":"Mai A. Saleh,&nbsp;Mohamed R. Habib,&nbsp;Sherif R. AbdElGhany,&nbsp;Mohamed Fathy Abouel-Nour,&nbsp;Hassan M. M. Masoud","doi":"10.1186/s43088-026-00759-6","DOIUrl":"10.1186/s43088-026-00759-6","url":null,"abstract":"<div><h3>Background</h3><p>Marine organisms, particularly snails, are rich sources of bioactive compounds with potential antioxidant, antimicrobial, and anticancer properties, offering promising avenues for drug discovery.</p><h3>Methods</h3><p>In this study, <i>Rapana venosa</i> extracts were evaluated for antioxidant enzyme activities and tested for antimicrobial and anticancer effects. Furthermore, a superoxide dismutase (SOD) isoenzyme, designated <i>Rapana venosa</i> superoxide dismutase 1 (RvSOD1), was purified from <i>R. venosa</i> using chromatographic techniques, characterized, and evaluated for its antimicrobial and anticancer activities.</p><h3>Results</h3><p>The crude <i>R. venosa</i> extract demonstrated substantial antioxidant activity, with measurable catalase (CAT), superoxide dismutase (SOD), glutathione S-transferase (GST), and glutathione peroxidase (GPx) activities. It exhibited antimicrobial effects, inhibiting <i>Escherichia coli</i> by 71.2% and <i>Candida albicans</i> by 90.8%. The extract also showed cytotoxicity against human cancer cell lines MCF-7 (breast cancer), MDA-MB-231 (triple-negative breast cancer), and A549 (lung adenocarcinoma). The purified RvSOD1 displayed a specific activity of 600.7 U/mg (7.5-fold purification; 30.4% yield). Characterization indicated that RvSOD1 is a copper–zinc SOD, sensitive to potassium cyanide and activated by Zn²⁺ and Cu²⁺, with an optimum pH of 8.0 and an apparent molecular mass of ~ 90 kDa. Functionally, RvSOD1 showed antifungal activity against <i>C. albicans</i> (79.9% inhibition) and antibacterial activity against <i>E. coli</i>, <i>Pseudomonas aeruginosa</i>, <i>Klebsiella pneumoniae</i>, and <i>Staphylococcus aureus</i>. In anticancer assays, RvSOD1 was active against MCF-7 and MDA-MB-231 cells but was less effective against A549 cells. Notably, the crude extract was more effective than purified RvSOD1 against all three cell lines, suggesting synergistic effects or additional bioactive compounds.</p><h3>Conclusion</h3><p>Both the <i>R. venosa</i> extract and the isolated RvSOD1 possess significant antioxidant, antimicrobial, and anticancer properties. These findings highlight their potential as promising multifunctional therapeutic candidates for future pharmaceutical and biomedical applications.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"15 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s43088-026-00759-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147642427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natural products as alternative therapies for eye disorders: from traditional use to modern formulations","authors":"Abdullah M. M. Elbadry,&nbsp;Shaza H. Aly,&nbsp;Nada A. Abdelrazek,&nbsp;Riham A. El-Shiekh,&nbsp;Aya Salman,&nbsp;Ahmed Nabil,&nbsp;Ahmed M. Reda,&nbsp;Omneya Eid,&nbsp;Eman G. Manaa,&nbsp;Sherif R. Abdel-All,&nbsp;Mai M. Farid","doi":"10.1186/s43088-026-00757-8","DOIUrl":"10.1186/s43088-026-00757-8","url":null,"abstract":"<div><h3>Background</h3><p>Eye disorders represent a global health challenge, requiring the exploration of natural products as alternative remedies.</p><h3>Main body</h3><p>This review establishes a critical bridge between traditional ethnobotanical knowledge and modern clinical ophthalmology to combat irreversible blindness. The analysis is unified by the pathological aspects of oxidative stress, chronic inflammation, and neurodegeneration common to both anterior and posterior ocular segments. We gathered data on plants traditionally used for diverse conditions, ranging from surface ailments (dry eye, keratitis, conjunctivitis, corneal wounds, blepharitis) to deep-tissue pathologies (cataract, glaucoma, macular degeneration, presbyopia, and diabetic retinopathy). The review culminates in a discussion on advanced formulations designed to overcome ocular barriers and enhance delivery. By integrating herbal with pharmacological insights, this work identifies research gaps and the potential for standardized natural products to preserve vision health worldwide.</p><h3>Conclusion</h3><p>This review underscores the therapeutic promise of ethnobotanical natural products in targeting oxidative stress, inflammation, and neurodegeneration across ocular pathologies. By elucidating their bioactive compounds and innovative delivery systems, we highlight the potential for standardized formulations as adjuncts or alternatives to conventional therapies for vision preservation.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"15 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s43088-026-00757-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147642491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methanolic extract of Citrus japonica fruits accelerates wound healing in rats: an experimental animal study and LC-HRESIMS metabolomic analysis","authors":"Ingy M. Hashad,&nbsp;Heba A. S. El-Nashar,&nbsp;Dalia O. Saleh,&nbsp;Nesma M. E. Abo El-Nasr,&nbsp;Marwa E. Shabana,&nbsp;Usama R. Abdelmohsen,&nbsp;Nada M. Mostafa,&nbsp;Ahmed M. Mostafa","doi":"10.1186/s43088-026-00758-7","DOIUrl":"10.1186/s43088-026-00758-7","url":null,"abstract":"<div><h3>Background</h3><p><i>Citrus japonica (C. japonica</i>), known as Kumquat, is small-size edible fruit native to South Asian regions and reported for its anti-inflammatory and antioxidant properties. This manuscript aimed to investigate LC-HRESIMS-based metabolomic profiling and wound healing properties of <i>C. japonica</i> fruit extract hydrogel formulation.</p><h3>Methods</h3><p>The metabolic profiling of the fruit extract was investigated using LC-HRESIMS. The extract was formulated in hydrogel preparation and examined for in vivo wound healing activity. Twenty rats were divided into four different groups (n = 5); the negative control group did not undergo any wound creation; the positive control group was wounded and did not receive daily application of a topical formulation based on the wound area; the reference group consisted of wounded rats treated with MEBO® ointment applied to the wound area. The remaining group of rats were wounded and received topical preparations of <i>C. japonica</i> with variations in the size of the applicator head. The effects of <i>C. japonica</i> fruit extract were examined on all phases of wound healing via assessment of the expression levels of key markers such as TNF-α, VEGF, PDGF, and TIMP-2. Besides, morphological and histopathological studies were conducted.</p><h3>Results</h3><p>The findings reveal that treatment with <i>C. japonica</i> extract expedites wound healing, showing comparable effectiveness to standard treatments like MEBO®. Understanding these molecular markers provides valuable insights for designing precise treatments that enhance the wound healing process and minimize complications in clinical settings. The LC-HRESIMS revealed the characterization of 21 secondary metabolites belonging to methoxylated flavonoids, phenolic acids, anthocyanins, and coumarins.</p><h3>Conclusion</h3><p><i>C. japonica</i> gel exhibits significant efficacy in wound healing in vivo and warrants further investigation for potential clinical applications.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"15 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s43088-026-00758-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147642490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}