Beni-Suef University Journal of Basic and Applied Sciences最新文献

{"title":"Therapeutic and organelle contact-site modulation theory in non-alcoholic fatty liver disease","authors":"Xinyi Kwan,&nbsp;Yujie Wang,&nbsp;Huiqing Liang,&nbsp;Ning Qu,&nbsp;Shaodong Chen,&nbsp;Muhammad Shahzad Aslam","doi":"10.1186/s43088-026-00754-x","DOIUrl":"10.1186/s43088-026-00754-x","url":null,"abstract":"<div><h3>Background</h3><p>Non-alcoholic fatty liver disease affects roughly one third of adults worldwide and is expected to rise further. Despite its growing burden, effective pharmacological therapies remain limited. Emerging evidence indicates that disruption of communication between intracellular organelles, particularly between the endoplasmic reticulum and mitochondria, plays a central role in hepatic lipid accumulation, oxidative stress, and metabolic dysfunction. Restoration of these inter-organelle contact sites may therefore represent a unifying therapeutic strategy.</p><h3>Main body</h3><p>This narrative review, guided by systematic search principles, examined published studies on selected medicinal herbs and their potential influence on organelle communication in non-alcoholic fatty liver disease. Six major databases were searched through December 2024. Twenty-eight in vivo, in vitro, and clinical studies met predefined inclusion criteria, focusing on seven botanicals widely used in traditional medicine. To complement thematic synthesis, abstracts were analyzed using computational text mining and network approaches to identify dominant mechanistic patterns. Across the literature, hepatic lipid metabolism and organelle stress emerged as central themes. Network analysis identified liver, lipid, and protein as highly connected concepts linking mechanistic and therapeutic domains. Topic modeling revealed four main research areas: phytochemical interventions, lipid droplet and organelle interface dynamics, metabolic profiling, and steatohepatitis models. Experimental findings indicate that several herbal compounds reduce steatosis, oxidative stress, and fibrosis while improving mitochondrial function and endoplasmic reticulum homeostasis. Lifestyle interventions such as aerobic exercise were also associated with improved lipid droplet and mitochondria interactions. However, most studies inferred modulation of organelle contact sites indirectly through downstream metabolic markers, and direct structural assessment remains limited.</p><h3>Conclusion</h3><p>Current evidence suggests that modulation of inter-organelle contact sites may represent a convergent mechanism underlying the hepatoprotective effects of selected medicinal herbs. Enzymes involved in phospholipid remodeling and proteins governing organelle tethering appear as promising but underexplored therapeutic targets. Further mechanistic and multi-omics investigations, supported by well-designed clinical studies, are needed to translate these insights into effective treatments for non-alcoholic fatty liver disease.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"15 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s43088-026-00754-x.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147642410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vivo efficacy of Zingiber officinale (Zingiberaceae), Punica granatum (Punicaceae), and Foeniculum vulgare (Apiaceae) extracts against Giardia lamblia trophozoites with evaluation of serum IgE levels","authors":"Abdulkarim Dakah,&nbsp;Ghassan Abdulrahaman,&nbsp;Mohammed Nour Al Kurdi,&nbsp;Yahya assany","doi":"10.1186/s43088-026-00753-y","DOIUrl":"10.1186/s43088-026-00753-y","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Background&lt;/h3&gt;&lt;p&gt;&lt;i&gt;Giardia lamblia&lt;/i&gt;, a protozoan parasite causing Giardiasis, that is epidemic in the developing countries, is commonly treated with drugs like metronidazole, though resistance and side effects drive interest in herbal alternatives.&lt;/p&gt;&lt;h3&gt;Methods&lt;/h3&gt;&lt;p&gt;The ethanolic extracts of Ginger (&lt;i&gt;Zingiber officinale&lt;/i&gt;), Pomegranate (&lt;i&gt;Punica granatum&lt;/i&gt;), and Fennel (&lt;i&gt;Foeniculum vulgare&lt;/i&gt;) were prepared and subjected to qualitative phytochemical analysis to detect the presence of alkaloids, flavonoids, saponins, tannins, and polyphenols. Stool samples were collected from patients and &lt;i&gt;Giardia lamblia&lt;/i&gt; cysts were isolated. For in vivo experiments, 36 four-week-old BALB/c mice were used. Each mouse was orally inoculated with 0.5 mL of the Giardia cyst suspension (9000 cysts/mL). Infection was confirmed by microscopic examination of fecal samples five days post inoculation. The mice were treated with 300 mg/kg of extracts for 7 days, and the anti-Giardia efficacy of extracts were tested by measuring cyst counts and reduction percentages over 20 days. Blood was drawn from the orbital vein after infection in mice and before treatment and again on day 14 of infection. Serum was obtained by centrifuging blood samples. IgE levels were measured using the Roche Elecsys device, which uses sandwich immunoassay technology that relies on streptavidin binding and electrochemiluminescence.&lt;/p&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;p&gt;All three plants share several phytochemical constituents (tannins, flavonoids, and polyphenols), but there are distinct differences in the presence of saponins and alkaloids among them. Early intervention (Day 5): Among all treatments, fennel showed the greatest initial cyst reduction (50.8%), followed in decreasing order by ginger (43.9%) and finally metronidazole (22.1%). Pomegranate behaved poorly in terms of showing an early effect. Mid-treatment (Day 10): Metronidazole was the foremost (88.1%), with fennel next (82.2%), followed in decreasing order by ginger (76.7%) and then pomegranate (69.5%). Day 20: Metronidazole remained the most effective (98.1%) while fennel (90.9%) and ginger (90.5%) were approaching similar efficacy, and pomegranate having attained (81.3%). IgE modulation: All extracts counteracted the elevated levels of IgE post-treatment; however, among all, pomegranate was the most potent in achieving a maximum reduction (45%), with ginger being next at (33%), followed by fennel at (30%) and metronidazole at (28%).&lt;/p&gt;&lt;h3&gt;Conclusion&lt;/h3&gt;&lt;p&gt;Fennel and ginger extracts show promising anti-Giardia activity, especially in cyst reduction, while pomegranate extract, although slower in initial antiparasitic action, excels in modulating immune response by significantly reducing IgE levels. These findings support the potential of these plant extracts as adjunct therapies for giardiasis, combining antiparasitic effects with immune modulation, which may enhance treatment outcomes and reduce reliance on convention","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"15 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s43088-026-00753-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147561458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biological control of Tetranychus urticae mite by predatory mites compared to pesticides on strawberry plants under field conditions","authors":"Manal A. Abdel Razik,&nbsp;Zamzam M. Al Dhafar,&nbsp;Mohamed A. Osman,&nbsp;Mohamed E. Sweelam","doi":"10.1186/s43088-026-00741-2","DOIUrl":"10.1186/s43088-026-00741-2","url":null,"abstract":"<div><h3>Background</h3><p>The two-spotted spider mite, <i>Tetranychus urticae</i> (<i>T. urticae</i>) is one of the most serious pests affecting strawberry plants. The use of various chemical pesticides has posed numerous environmental risks, necessitating the search for alternative solutions that are safer and more sustainable, such as the use of biological control agents, which offer an effective and environmentally friendly method in pest management.</p><h3>Methods</h3><p>This study was conducted to compare the biological control of the two-spotted spider mite by releasing predatory mites three times over two successive seasons, comparing to three pesticide systems applied by two methods as well as, to estimate the impact of the release processes and applied pesticides on strawberry yield.</p><h3>Results</h3><p>The predatory mites: <i>Phytoseiulus persimilis</i> (<i>P. persimilis</i>), and <i>Neoseiulus californicus</i> (<i>N. californicus</i>), demonstrated significant efficacy in reducing populations of <i>T. urticae</i> on strawberry plants during 2023 and 2024 seasons. <i>P. persimilis</i> outperformed the other species, recording the lowest numbers of eggs and motile stages of the pest, with reduction rates of 81.7% and 84.9% in the 2023 season, and 72.6% and 76.6% in the 2024 season. <i>N. californicus</i> ranked second in effectiveness, with reduction rates of 78.4% and 74.6% in the 2023 season, and 68.9% and 70.4% in the 2024 season. The chemical treatment of (thiamethoxam, micronized sulfur, and mineral oil) also showed good efficacy, particularly when applied as a foliar spray, with reduction rates of 85.7% and 81.5% in the 2023 season, and 68.1% and 71.4% in the 2024 season. Regarding strawberry yield, the biological treatment with <i>P. persimilis</i> achieved the highest increase in yield, with 38.7% in the 2023 season and 39.7% in the 2024 season. Among the chemical treatments, the foliar spray of thiamethoxam, micronized sulfur, and mineral oil resulted in the highest yield increase, with 46.2% in the 2023 season and 44% in the 2024 season.</p><h3>Conclusions</h3><p>It could be recommend the use of <i>P. persimilis</i> and <i>N. californicus</i> predators along with the treatment of (thiamethoxam-micronized sulfur-mineral oil) to control <i>T. urticae</i> infestation on strawberry plants under field conditions.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"15 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s43088-026-00741-2.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147561459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From wheat to wheat: synergistic effect of silica nanoparticles synthesized from wheat straw assisted by gamma irradiation against khapra beetle, Trogoderma granarium everts on wheat grain","authors":"Sherif Hamed,&nbsp;Rehab M. Sayed,&nbsp;Shaimaa A. A. Mo’men,&nbsp;Asmaa Sayed,&nbsp;Emad M. S. Barakat","doi":"10.1186/s43088-026-00751-0","DOIUrl":"10.1186/s43088-026-00751-0","url":null,"abstract":"<div><h3>Background</h3><p><i>Trogoderma granarium</i> Everts (Coleoptera: Dermestidae) is one of the most deleterious post-harvest pests of wheat grain in the first place and many other stored commodities. Sustainable, environmentally safe alternatives to chemical control methods are urgently needed, especially for food materials. Therefore, this study proposes a novel approach that utilizes wheat straw, an agricultural waste, to synthesize amorphous silica nanoparticles (SiO₂NPs) and enhance their efficacy by exposing them to different doses of gamma irradiation.</p><h3>Methods</h3><p>Silica nanoparticles (SiO₂NPs) were synthesized and exposed to different gamma irradiation doses (25, 50, 100 and 200 kGy). Characteristics of the as-synthesized SiO₂NPs were evaluated using different characterization techniques such as Fourier transform infrared (FTIR), Transmission electron microscope (TEM), X-ray diffraction (XRD), energy-dispersive X-ray analysis spectrometer (EDX), electron spin resonance (ESR), thermal gravimetric analysis (TGA) and Brunauer–Emmer–Teller analysis (BET). Bioassay experiments were conducted at four concentrations (0.5, 1, 2 and 4 mg per treatment) to evaluate the insecticidal activity of the as-synthesized SiO₂NPs using the median lethal concentration (LC₅₀) for both unirradiated and gamma-irradiated insects and wheat group at a sublethal dose of 100 Gy.</p><h3>Results</h3><p>The 50-kGy-irradiated SiO₂NPs (50kGy_SiO₂NPs) showed the most prominent characteristics, such as the uniform dispersion, a small particle size (5.4 ± 0.04 nm) and the highest surface area (148.70 m²/g). The combination of 50 kGy SiO₂NPs with 100-Gy-irradiated insects showed the most potent insecticidal activity with the lowest LC₅₀. A powerful synergistic effect was observed when (4 mg/5 g) of 50kGy_SiO₂NPs was combined with the 100-Gy-irradiated insects, resulting in 95% and 100% larval mortality at 7 and 14 days post-treatment, respectively, accompanied by 100% antifeedant percentage, pupal inhibition and complete suppression of adult emergence.</p><h3>Conclusion</h3><p>Combining gamma-optimized SiO₂NPs with sublethal irradiation of insects can provide a potent, reliable and eco-friendly approach for the integrated pest management of stored grain pests. Such a combination aims to reduce the applied amount of SiO₂NPs and lower the irradiation dose required for controlling <i>T. granarium</i> in their separate usage. To our knowledge, this study is the first to report the synergistic insecticidal effect of gamma-irradiated SiO₂NPs derived from wheat straw for <i>T. granarium</i> control.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"15 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s43088-026-00751-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147561533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Data-efficient AI models for aerodynamic coefficient prediction: a case study on NACA airfoils with minimal CFD runs","authors":"Abdelrahman Mostafa,&nbsp;Mohamed Adel,&nbsp;Mira M. Suliman,&nbsp;Mofreh Milad,&nbsp;Amr A. Zamel","doi":"10.1186/s43088-026-00750-1","DOIUrl":"10.1186/s43088-026-00750-1","url":null,"abstract":"<div><h3>Background</h3><p>High-fidelity computational fluid dynamics (CFD) remains a bottleneck during early aerodynamic design, where many candidate configurations must be screened under strict computational budgets. Data-efficient artificial intelligence (AI) surrogates offer a promising solution to recover aerodynamic coefficients with minimal simulation effort. Within this context, achieving comparable predictive performance using a fraction of the CFD data is essential for efficient surrogate-based design exploration. To reflect real aerodynamic design constraints, this study explicitly investigates how far CFD-generated datasets can be reduced while maintaining predictive reliability, establishing a quantitative balance between accuracy and computational cost.</p><h3>Methods</h3><p>A validated dataset was generated using Reynolds-averaged Navier–Stokes (RANS) simulations with the Spalart–Allmaras turbulence model for four NACA airfoil families, covering a range of Reynolds numbers and angles of attack. To emulate resource-constrained scenarios, the dataset was systematically reduced to 30%, 25%, 20%, 15%, 10%, and 5% of its original size. Ten widely used machine learning algorithms were benchmarked against feedforward backpropagation neural networks with varying hidden layer sizes.</p><h3>Results</h3><p>Across the tested configurations, models maintained strong predictive performance even with substantially reduced datasets. At a 10% training ratio, some models achieved <i>R</i>² values up to 0.98398 with low mean absolute error, while simulation time decreased from approximately 36 h 40 min to about 3 h 45 min. However, reducing the dataset to 5% resulted in a measurable decline in accuracy, particularly for lift predictions and for deeper neural networks.</p><h3>Conclusions</h3><p>This study demonstrates that AI surrogates can be integrated into CFD workflows to significantly reduce computational cost while preserving predictive accuracy. The findings establish a practical framework for dataset reduction, paving the way for extending this methodology to broader aerodynamic configurations in future studies.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"15 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s43088-026-00750-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147561602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anticancer, antimicrobial, and probiotic properties of bacterial strains derived from edible snails Cornu aspersum and Eobania vermiculata gut microbiota","authors":"Reham Alaa Eldin Shaker,&nbsp;Rasha A. Hashem,&nbsp;Mariam Hassan,&nbsp;Amina M. Ibrahim,&nbsp;Yasser M. Ragab,&nbsp;Rania Abdelmonem Khattab","doi":"10.1186/s43088-026-00747-w","DOIUrl":"10.1186/s43088-026-00747-w","url":null,"abstract":"<div><h3>Background</h3><p>Land snails, a diverse and ecologically important group under the class Gastropoda, possess unique gut microbial communities that are crucial to their physiology, ecology, and environmental interactions. To explore the intestinal microbiota of land snails, <i>Cornu aspersum</i> (<i>C. aspersum</i>) and <i>Eobania vermiculata</i> (<i>E. vermiculata</i>) were obtained from their natural habitat in agricultural environments and investigated in this study.</p><h3>Results</h3><p>The results demonstrated that the phyla <i>Pseudomonadota, Bacteroidota,</i> and <i>Actinomycetota</i> dominated land snails’ guts, comprising 82–96%, 13.7–3.5%, and 2–0.5% of the total relative abundance in <i>C. aspersum</i> and <i>E. vermiculata,</i> respectively. Through <i>16S rRNA</i> gene sequencing, two bacterial strains were isolated from land snails’ gut microbiota and identified as <i>Staphylococcus xylosus</i> (<i>S. xylosus</i>) and <i>Enterococcus hirae</i> (<i>E. hirae</i>)<i>.</i> The two bacterial strains exhibited probiotic properties such as persistence in acidic pH (2.5) and bile salts (0.7% and 0.3%) and demonstrated antibacterial properties against pathogenic bacteria. In addition, both isolated bacterial strains demonstrated anticancer activity against Caco-2 cells. The production of exopolysaccharide (EPS) from the isolated bacteria was quantified, showing higher quantities produced by <i>E. hirae</i>.</p><h3>Conclusions</h3><p>This investigation demonstrated the intestinal microbiota biodiversity of terrestrial snails <i>C. aspersum</i> and <i>E. vermiculata</i> and their use as a niche source for beneficial bacteria.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"15 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s43088-026-00747-w.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147561721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Network pharmacology and computational dissection of Solanum trilobatum bioactives reveals modulation of steroid receptor associated signalling in breast cancer","authors":"Prarambh S. R. Dwivedi,&nbsp;A. Muthu Bharathi,&nbsp;Wilona Loren Lobo,&nbsp;C. S. Shastry,&nbsp;Akhil Nair,&nbsp;Akhilesh Dubey,&nbsp;Vijishna Lekshmi Viswambharan,&nbsp;Pavithra Pradeep Prabhu","doi":"10.1186/s43088-026-00756-9","DOIUrl":"10.1186/s43088-026-00756-9","url":null,"abstract":"<div><h3>Background</h3><p>Breast cancer is the most common form of malignancy in women worldwide. Although chemotherapy has been improved, the major drawbacks demand alternative treatment plans. Bioactives based on plants have become a key focus of interest as complementary agents and <i>Solanum trilobatum</i> has been known to possess multiple pharmacological activities that include, antioxidant, antimicrobial, anti-inflammatory, and anti-cancer effects. The molecular paths of action of its anticancer effect, however, is mostly unexplored.</p><h3>Methods</h3><p>An integrative in silico strategy combining network pharmacology, molecular docking, molecular dynamics (MD) simulations, and binding free energy calculations was employed to elucidate the anticancer mechanisms of <i>S. trilobatum</i> bioactives. Phytoconstituents were mined from curated databases and screened based on drug-likeness, ADMET properties, and predicted anti-neoplastic activity. Protein protein interaction networks, KEGG pathway enrichment, and Gene Ontology analyses were performed to identify key pathways and hub genes. Selected bioactives were docked against oncogenic targets using AutoDock Vina and Schrödinger Glide. The most promising protein ligand complexes were further validated through long-timescale MD simulations and MMPBSA binding free energy analysis.</p><h3>Results</h3><p>The enrichment of the analysis by KEGG pathways demonstrated the PI3K-Akt signalling pathway as the pathway that was massively altered. Network analysis identified AKT1, TP53, TNF, STAT3, MYC, IL6, HIF1A, EGFR, CTNNB1, and CASP3 as hub genes. Molecular docking of rheochrysin, hypoxiside, cytochalasin and 4- p- coumatoylquinic acid to oncogenic proteins, HRAS, KRAS, NCOA3 (a nuclear coactivator of steroid receptors), PGR, and PIK3CB oncogenes was observed using AutoDock 4.2 and Schrodinger Glide with binding affinities displaying strong binding. Molecular dynamics simulations further confirmed stable and sustained interactions, particularly between KRAS–4-p-coumaroylquinic acid and TP53–Hypoxoside A complexes.</p><h3>Conclusion</h3><p>These findings provide mechanistic insights into the anticancer potential of <i>S. trilobatum</i>, supporting its role as a promising source of bioactive compounds capable of modulating steroid receptor–associated signalling networks in breast cancer therapy.</p><h3>Graphic abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"15 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s43088-026-00756-9.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147560347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effects of propolis against bisphenol a-induced hepatorenal toxicity in male rats: an experimental study","authors":"Nehal H. Ali,&nbsp;Heba N. Gad EL-Hak,&nbsp;Zohour I. Nabil,&nbsp;Ghada M. Abd Elghany","doi":"10.1186/s43088-026-00744-z","DOIUrl":"10.1186/s43088-026-00744-z","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Background&lt;/h3&gt;&lt;p&gt;Bisphenol A (BPA), a ubiquitous environmental endocrine disruptor, induces multisystem toxicity primarily through oxidative stress and activation of inflammatory pathways. Propolis, a natural bee-derived product rich in polyphenolic compounds, exhibits potent antioxidant and anti-inflammatory properties. This study investigated the protective efficacy of aqueous propolis against BPA-induced hepatorenal dysfunction in male rats.&lt;/p&gt;&lt;h3&gt;Methods&lt;/h3&gt;&lt;p&gt;Forty-two male Sprague–Dawley rats (90–120 g) were randomly allocated into seven groups (&lt;i&gt;n&lt;/i&gt; = 6): control (saline), vehicle (olive oil; 5 mL/kg), BPA (10 mg/kg), propolis-low (125 mg/kg), propolis-high (250 mg/kg), BPA + propolis-low, and BPA + propolis-high. Treatments were administered orally once daily for eight weeks. Hematological parameters, hepatic enzymes (ALT, AST), renal function markers (creatinine, urea), electrolytes (Na&lt;sup&gt;+&lt;/sup&gt;, K&lt;sup&gt;+&lt;/sup&gt;, Ca&lt;sup&gt;2+&lt;/sup&gt;), oxidative stress biomarkers (MDA, TAC, NO, protein carbonyl), inflammatory cytokines (IL-6, TNF-α, IFN-γ), and tissue trace elements were quantified using standardized methodologies. Liver and kidney tissues were processed for histopathological examination using hematoxylin and eosin (H&amp;E) staining, and pathological changes were graded based on the percentage of affected tissue area.&lt;/p&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;p&gt;BPA exposure induced significant hepatorenal toxicity, characterized by elevated transaminases, impaired renal function, and electrolyte imbalance. Profound oxidative stress and inflammatory activation were observed, accompanied by a decline in hemoglobin concentration. Histopathological examination revealed severe hepatic damage, including hepatocyte degeneration and necrosis, while renal histology showed extensive tubular epithelial necrosis, tubular dilatation with proteinaceous casts, glomerular congestion, interstitial edema, and inflammatory cell infiltration. Propolis co-administration conferred dose-dependent protection, with the high-dose regimen (250 mg/kg) restoring hemoglobin to near-normal levels, completely normalizing creatinine, reducing MDA, restoring TAC, and significantly lowering IL-6 and TNF-α levels. Histopathological analysis confirmed the protective role of propolis; high-dose treatment preserved normal hepatic architecture with minimal degenerative changes. Hepatic zinc accumulation was evident in the high-dose group, potentially contributing to the activation of antioxidant enzymes.&lt;/p&gt;&lt;h3&gt;Conclusions&lt;/h3&gt;&lt;p&gt;Chronic BPA exposure induces severe hepatorenal dysfunction, oxidative stress, inflammatory activation, and hematological disturbances in male rats, as evidenced by biochemical and histopathological alterations. Propolis supplementation, particularly at 250 mg/kg, demonstrated marked protective efficacy by scavenging free radicals, restoring antioxidant capacity, suppressing inflammatory mediators, and modulating trace elements. These effects were accompanied ","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"15 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s43088-026-00744-z.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147441327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Archachatina marginata slime extract predisposes to oxidative stress following sub-acute oral exposure in rats","authors":"Morayo Barnabas,&nbsp;Damilare Rotimi,&nbsp;Tobiloba Maduakolam-Aniobi,&nbsp;Amarachi Iroaganachi,&nbsp;Funmilayo Okeniyi,&nbsp;Oluwakemi Awakan,&nbsp;Oluyomi Adeyemi","doi":"10.1186/s43088-026-00752-z","DOIUrl":"10.1186/s43088-026-00752-z","url":null,"abstract":"<div><h3>Background</h3><p><i>Archachatina marginata</i>, the giant African land snail, is a terrestrial mollusk native to West Africa and commonly found in Nigeria and other tropical regions. Over the years, it has gained popularity not only as a source of protein but also for its slime, which is increasingly utilized in traditional medicine and the cosmetic industry. The slime, a viscous secretion produced by the snail’s foot, is rich in glycoproteins, hyaluronic acid, antimicrobial peptides, and antioxidants. Traditionally, it has been applied topically to treat wounds, skin infections, and burns, while its oral use is gaining attention in folk remedies and as a perceived natural health booster. Despite these claims, the scientific evaluation of the safety profile of <i>A. marginata</i> slime, particularly upon oral ingestion, remains limited. This study evaluated the sub-acute oral impact of <i>A. marginata</i> slime extracts on rat hematology, redox status, and pro-inflammatory cytokine expression.</p><h3>Method</h3><p>The grouping of rats (5 animals per group) was random, comprising a control group and treatment groups receiving <i>A. marginata</i> slime extract at doses of 125, 250, or 500 mg/kg body weight for 28 days. A follow-up group received 500 mg/kg and was observed for an additional 14 days post-treatment. Following exposure, animals were euthanized, and blood and key organs were collected for biochemical analysis.</p><h3>Results</h3><p>In male rats, the 250 mg/kg dose led to a notable increase in malondialdehyde (MDA) levels and a decrease in reduced glutathione (GSH), suggesting that the slime extract may induce oxidative stress at this dosage. Female follow-up group exhibited elevated MDA and CRP (C-reactive protein) levels. Notably, slime extract had no effect on rat hematological profile and plasma levels of Nrf-2 (nuclear factor erythroid 2-related factor 2), NF-κB (nuclear factor kappa B), and TNF-α (tumor necrosis factor alpha). Chemical analysis identified predominant compounds like allantoin and glycolic acid, endorsing the cosmetic potential of the slime.</p><h3>Conclusion</h3><p>Overall, these findings contribute to understanding the toxicity and safety profile of <i>A. marginata</i> slime extract in rats.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"15 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s43088-026-00752-z.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147441033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytoconstituents and bioactivities among different parts of Ardisia humilis Vahl.: antioxidant activity and anticancer potential via in vitro and molecular docking approaches","authors":"Maya Damayanti Rahayu,&nbsp;Devi Permatasari,&nbsp;Galih Kusuma Aji,&nbsp;Susi Kusumaningrum,&nbsp;Siska Andrina Kusumastuti,&nbsp;Ngatinem Ngatinem,&nbsp;Agus Himawan,&nbsp;Nurhadi Nurhadi,&nbsp;Rudi Heryanto,&nbsp;Alfi Hudattul Hasanah,&nbsp;Agung Eru Wibowo","doi":"10.1186/s43088-026-00749-8","DOIUrl":"10.1186/s43088-026-00749-8","url":null,"abstract":"<div><h3>Background</h3><p><i>Ardisia humilis Vahl.</i> belongs to the genus <i>Ardisia</i>, which possesses secondary metabolites. This study investigates the phytoconstituents, antioxidant, and cytotoxic properties of different parts of Ardisia humilis Vahl.</p><h3>Methods</h3><p>Different parts (leaf, stem, seed, flower, and young fruit) of <i>Ardisia humilis Vahl.</i> were extracted using methanol and investigated for the antioxidant and cytotoxic properties in human breast cancer cells (Michigan Cancer Foundation-7/MCF-7). In addition, biochemical profiling was performed using ultra-high-performance liquid chromatography coupled with high-resolution mass spectrometry (UPLC-HRMS). Moreover, molecular docking studies on estrogen receptor alpha (Erα) (PDB ID = 6CBZ) and fatty acid synthase (FASN) (PDB ID = 4PIV) were performed.</p><h3>Results</h3><p>The leaf extract exhibited the highest antioxidant activity (IC₅₀ = 32.869 µg/mL), followed by the stem, seed, young fruit, flower, and old fruit. The highest phenolic content (155.07 mgGAE/g extract) was observed in the leaf extract. The highest total flavonoid content (TFC) was obtained from the stem extract (46.97 mgQE/g extract). A total of 38 compounds were putatively identified across all different parts of <i>Ardisia humilis Vahl.</i> via UPLC-HRMS. In addition, cytotoxicity assays on human breast cancer (MCF-7) cells demonstrated that the seed and leaf extracts significantly inhibited cancer cell proliferation by 101.74 and 92.56%, respectively, at 200 µg/mL. Molecular docking revealed that 4',4-dimethylepigallocatechingallate, predominantly found in leaf extract, had the strongest binding affinity to Erαwith a MolDock score of − 145.667 kcal/mol, surpassing the positive control 4-hydroxytamoxifen (− 116.207 kcal/mol). Meanwhile, tiliroside, also identified in the leaf, exhibited the best score docking to FASN with a MolDock score (− 172.372 kcal/mol), outperforming the known inhibitors IPI-9119 and cerulenin.</p><h3>Conclusion</h3><p>This study establishes <i>Ardisia humilis Vahl.</i> plant parts as a significant source of antioxidant and anticancer potential. Further studies are warranted to isolate, characterize, and elucidate the bioactive mechanisms through comprehensive in vitro and in vivo investigations.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"15 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s43088-026-00749-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147363101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}