Beni-Suef University Journal of Basic and Applied Sciences最新文献

{"title":"Effectiveness of lime kiln dust on swelling of subgrade expansive soil","authors":"Mennat-allah Eid,&nbsp;Youssef Gomaa,&nbsp;Sameh Galal","doi":"10.1186/s43088-024-00501-0","DOIUrl":"10.1186/s43088-024-00501-0","url":null,"abstract":"<div><h3>Background</h3><p>The structure of flexible or rigid pavement built on expansive subgrade soil that has a volumetric change is vulnerable to many problems that might cause failure. Pavement and construction became more durable and economical by enhancing the quality of subgrade expansive soil. Solid waste recycling has become very popular recently as a means of attaining sustainable waste management, so using lime kiln dust (LKD), which is a by-product of quick lime production, to treat expansive soil in pavement subgrades. This research describes the effect of LKD on the chemical composition, strength, and swelling of high and low-plastic clay that were extracted from two sites. The minimum LKD required for treating expansive soils was determined by using the Eades and Grim pH test. From tests, it was found that the addition of LKD increased the shrinkage limit by a range (250–500)% and decreased the plasticity and swelling potential by between (50 and 100)% of expansive subgrade soils. The strength according to CBR, increased approximately by 150% for CL soil and 800% for CH soil.</p><h3>Results</h3><p>The optimal percentage of LKD for CH soil is 6%, and for CL soil, it is 2%. The plastic limit increased by 50% for CH soil at 6% LKD. On the other hand, CL soil became non-plastic at 4% LKD. With an increase in the percentage of LKD, it led an the increase in the shrinkage limit by 500% in CH soil and 250% in CL soil. The free swell decreased by 50% in CH soil and 100% in CL soil. The swelling pressure decreased by 50% for two expansive soils. CBR increased by 800% in CH soil and by 150% in CL soil.</p><h3>Conclusion</h3><p>This work found that the addition of LKD improves the physical, chemical, and mechanical properties of expansive subgrade soil.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"13 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-024-00501-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140924793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Space/ground-borne techniques and petrographic microscopic dissection for geologic mapping in Gabal Ras Abda area, Northeastern Desert, Egypt","authors":"Mahmoud M. Salem,&nbsp;Mohamed A. Yehia,&nbsp;Ali A. Omran,&nbsp;Hassan I. El Sundoly,&nbsp;Mohammed A. Soliman,&nbsp;Karim Abdelmalik","doi":"10.1186/s43088-024-00502-z","DOIUrl":"10.1186/s43088-024-00502-z","url":null,"abstract":"<div><h3>Background</h3><p>Gabal Ras Abda area as a part of the Red Sea Mountain range, is characterized by inaccessible and rugged terrains. The exposed rock units are hardly followed in the field because of the rigid topography. Thus, the present work proposes and develops an integrated approach to map the exposed rock units and extract the geologic structures using satellite imagery data followed by both field and petrographic verification, saving time, efforts and cost.</p><h3>Results</h3><p>To achieve the target, both the measured spectral signature curves with Landsat-8 and Sentinel-2A data were used to develop and create the most enhanced Band Ratios and Principal Components for lithological discrimination and mapping which were (((Band7 + Band 4)/(Band 7), (Band 2)/(Band 2 + Band 5) and (Band 5) in RGB) and ((Band 9 + Band 11 + Band 12)/(Band 1), (Band 4 − Band 2) and (Band 11/Band 6) + (Band 6) in RGB)) with Principal Component Bands ((PC1, PC2 and PC3 in RGB) and (PC3, PC2 and PC1 in RGB)), respectively. Also, georeferenced Google Earth Pro, panchromatic band of Landsat-8 and ALOS PALSAR Digital Elevation Model images were used to extract the structural lineaments. Geologic, petrographic and field structural studies were emphasized the remote sensing results, indicating that the main rock types cropped out in Ras Abda area from the oldest to the youngest are older granitoids (quartz-diorites, tonalites and granodiorites), Dokhan volcanics (andesites, rhyodacites, rhyolites and their related tuffs), younger gabbros, younger granites (monzogranites, syenogranites and alkali-feldspar granites), post-granite dykes and offshoots (acidic, microgranitic and basic types) and Phanerozoic sedimentary rocks. Also, the study emphasized that the E-W trend is the main structural trend controlling the investigated area followed by WNW-ESE and NE-SW directions.</p><h3>Conclusions</h3><p>The results of remote sensing achieved compliance with the geologic, petrographic and structural investigation through distinctly differentiating the different rocks and extracting the lineaments, indicating the accuracy of the remote sensing results and emphasizing their importance and effective role in producing a precise and highly accurate geologic map.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"13 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-024-00502-z","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140906945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative study of the reptilian cornea's microstructure","authors":"Zeinab Abdelftah,&nbsp;Ahmed Ragab,&nbsp;Rasha E. Abo-Eleneen,&nbsp;Abdelaziz S. Abuelsaad,&nbsp;Ahlam M. EL-Bakry","doi":"10.1186/s43088-024-00485-x","DOIUrl":"10.1186/s43088-024-00485-x","url":null,"abstract":"<div><h3>Background</h3><p>The cornea of various vertebrate is considered a major part in the glop, which acts as a powerful lens, providing a sharp retinal image, and meantime acts as an excellent defensor for other corneal layers.</p><h3>Results</h3><p>The four reptilian families [Scincidae (<i>Chalcides ocellatus</i>); Chamaeleontidae (<i>Chameleon chameleon</i>); Cheloniidae (<i>Chelonia mydas</i>) and Testudiniae (<i>Testudo kleinmanni</i>)] investigated in the current study were gathered across Egypt. After being taken out of the orbit, the cornea under inquiry was ready for light, specific stain and scanning electron microscopy. The epithelium, stroma, and endothelium are the three corneal layers that are common to all four species of reptiles. All other species lack Bowmen's and Descemet's membranes, with the exception of <i>C. ocellatus</i> and <i>T. kleinmanni</i>. The latter layers of <i>Chalcides ocellatus</i> display a strong affinity for Periodic Acid Schiff stain. Epithelial cells with a variety of forms, from hexagonal to atypical polygonal cells, cover the outer corneal surface. These epithelial cells are coated in short microplicae, microvilli, and microholes of varied diameters. There are a few blebs scattered around their surface.</p><h3>Conclusion</h3><p>The current study concluded that various habitats have various significant effects on the cornea's microstructure characteristics and its physiological trends to accommodate different environmental surroundings.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"13 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-024-00485-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140895167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interaction between HOTTIP, H19, and HOTAIR long noncoding RNAs and miRNA-152 in cases of HCC caused by HCV infection","authors":"Rady E. El-Araby,&nbsp;Fawzy Roshdy,&nbsp;Mariam Zaghloul,&nbsp;Ahmed A. E. Saad,&nbsp;Maha H. Morsi,&nbsp;Wafaa M. Radwan,&nbsp;Rana M. Adel,&nbsp;Sara H. Elshafiey,&nbsp;Yasmine Elhusseny,&nbsp;Reham F. Othman,&nbsp;Hamed Helal,&nbsp;Doha E. Hassanein,&nbsp;Hany A. Elghobary","doi":"10.1186/s43088-024-00499-5","DOIUrl":"10.1186/s43088-024-00499-5","url":null,"abstract":"<div><h3>Background</h3><p>Liver cancer (hepatocellular carcinoma “HCC”) remains a significant health issue without prompt detection and appropriate prevention. By interacting with each other, long noncoding RNAs (lncRNAs) and microRNAs (miRNAs) can form gene regulatory networks. Specifically, we aim to determine whether the lncRNAs (HOTTIP, H19, and HOTAIR) and miRNA-152 interact in a significant manner in the progression of Hepatitis C virus (HCV) patients to HCC. This is followed by the question of whether these biomarkers can be used to diagnose and prognose noninvasively. We used online computational techniques to predict which miRNA group is likely to affect the lncRNAs being examined. This study involved 133 participants. 103 patients with HCV were included in the study, which was divided into two groups: Group I, with 65 cases of chronic liver disease without HCC, and Group II, with 38 cases of chronic liver disease with HCC. In addition, 30 healthy volunteers served as controls. In this study, a qRT-PCR was used to test gene expression.</p><h3>Results</h3><p>A consistent reverse correlation has been observed between lncRNAs and miRNA-152 as the disease progresses.</p><h3>Conclusion</h3><p>According to our findings, the studied biomarkers may be useful as noninvasive biomarkers for prognosis in patients with HCV Genotype 4 who develop liver cirrhosis and HCC. Many miRNAs, including miRNA-19a and miRNA-106a, may interact with lncRNAs that have been investigated in addition to miRNA-152.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"13 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-024-00499-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140895173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of single and combined administration of ubiquinone and lactoferrin on histopathology, ultrastructure, oxidative stress, and WNT4 expression gene induced by thioacetamide on hepatorenal system of adult male rats","authors":"Sohaila Abd El-Hameed,&nbsp;Iman Ibrahim,&nbsp;Walaa Awadin,&nbsp;Ahmed El-Shaieb","doi":"10.1186/s43088-024-00494-w","DOIUrl":"10.1186/s43088-024-00494-w","url":null,"abstract":"<div><h3>Background</h3><p>Hepatorenal syndrome is a life-threatening medical complication of liver cirrhosis. Hepatic cirrhosis is commonly accompanied by rapid failure of renal functions. Thioacetamide (TAA) is a potent hepatotoxin and a class 2-type carcinogen. Ubiquinone (Coq₁₀) and lactoferrin (LF) are potent antioxidants with antifibrotic and antiinflammatory effects. However, whether Coq₁₀ and LF reduce the hepatorenal injury induced by TAA remains unclear. Here, we investigated the potential protective effect of both/or Coq₁₀ and LF in ameliorating TAA-induced hepatorenal injury and the role of WNT4 gene expression in detecting TAA-induced renal injury in rats. Seventy healthy and mature male Sprague Dawley rats, weighting (200 g ± 20 g) and aging (4–6) weeks were randomly divided into seven groups (<i>n</i> = 10): control, Coq₁₀, LF, TAA, TAA + Coq₁₀, TAA + LF, and TAA + Coq₁₀ + LF. The hepatorenal injury was induced through intraperitoneal (i.p.) injection of TAA (150 mg/kg/twice/weekly) for nine weeks. Coq₁₀ (10 mg/kg/day) and LF (200 mg/kg/day) were orally administered for nine weeks.</p><h3>Results</h3><p>TAA induced marked hepatorenal damage, evident by the significant increase in the alanine aminotransferase (ALT), aspartate transaminase (AST), serum creatinine (SCr) activities, and the blood urea nitrogen (BUN) level. Besides, the significant increases in concentrations of malondialdehyde (MDA) and nitric oxide (NOx) together with significant decreases in the activities of catalase (CAT) and superoxide dismutase (SOD). The histopathological analysis of the TAA group showed obvious fibrosis, steatosis, and inflammation of the hepatic parenchyma as well as severe glomerular and tubular damage of the renal parenchyma. In addition, TAA induced marked ultrastructural alterations and up-regulation in the expression of the WNT4 gene in the kidney. Meanwhile, the biochemical, histopathological, and ultrastructural alterations were significantly decreased with significant down-regulation in the expression of WNT4 in the groups exposed to TAA and treated with Coq₁₀ and LF.</p><h3>Conclusion</h3><p>Our data suggested that Coq₁₀ and LF could have protective effects on TAA hepatorenal damage, through improving the hepatic and renal functions, reduction of oxidative stress, structural and ultrastructural alterations, besides down-regulation in the expression of WNT4.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"13 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-024-00494-w","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140826086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic characterization and pathogenic effects of Hepatozoon canis infection in police dogs in Egypt","authors":"Olfat A. Mahdy,&nbsp;Marwa M. Khalifa,&nbsp;Ahmed A. Zaki,&nbsp;Asmaa K. Al-Mokaddem,&nbsp;Marwa M. Attia","doi":"10.1186/s43088-024-00493-x","DOIUrl":"10.1186/s43088-024-00493-x","url":null,"abstract":"<div><h3>Background</h3><p><i>Hepatozoon canis</i> is a protozoan parasite transmitted to dogs through ingesting the arthropod vector (hard ticks), which contains mature protozoal oocysts harboring infectious sporozoites.</p><h3>Aims</h3><p>This study aims to evaluate the blood parameters, biochemical assays and histopathological appraisal of infected police dogs with <i>Hepatozoon canis</i>, from kennels in the police academy of Egypt during 2020–2021.</p><h3>Methods</h3><p>Red blood cells count, hemoglobin, hematocrit, blood platelets and white blood cells count from collected blood samples were analyzed, and serum albumin, creatinine, urea, aspartate aminotransferase and alanine aminotransferase were analyzed from serum samples. Polymerase chain reaction amplified the 18S ribosomal RNAgene of the <i>Hepatozoon</i> species for genetic analysis, and the deoxyribonucleic acid products were sequenced and added to GenBank.</p><h3>Results</h3><p>The present study resulted in 5% of the police dog population being infested with <i>Rhipicephalus sanguineus</i>. This study registered the sequences of the <i>Hepatozoon canis</i> 18S ribosomal RNAgene in Egypt for the first time in Genbank (MW362244.1–MW362245.1). The biochemical assay revealed that the parasite severely affected the protein, significantly increasing serum albumin in positive polymerase chain reaction testing dogs.</p><h3>Conclusion</h3><p>A thorough inspection discovered that 100 police dogs had clinical symptoms like fever, emaciation and anemia, while the other 200 were healthy and had no evident clinical indicators.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"13 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-024-00493-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140820446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical composition, antioxidant, cytotoxic, antiviral, and lung-protective activities of Salvia officinalis L. ethanol extract herb growing in Sinai, Egypt","authors":"Asmaa A. Amer,&nbsp;Samar H. Kassem,&nbsp;Mohammed Abdalla Hussein","doi":"10.1186/s43088-024-00498-6","DOIUrl":"10.1186/s43088-024-00498-6","url":null,"abstract":"<div><h3>Background</h3><p>Pulmonary fibrosis (PF) is a respiratory ailment that causes the substitution of healthy lung tissue with scar tissue due to collagen deposition and fibroblast proliferation. The incidence of PF increased with the successive waves of Coronaviruses and other viruses affecting the lungs. Therefore, the priority is moving toward treatment with medicinal plants for their active constituents. <i>Salvia officinalis</i> L. herbal drug (sage, family; Lamiaceae) is characterized by its strong antioxidative activity. This study aims to declare the effect of using sage ethanol extract (SOEE) as a protective agent from PF through an in vivo study on mercuric chloride<b> (</b>HgCl₂)-induced lung fibrosis and in vitro evaluation of its anticancer, antiviral, and antioxidant activities concerning its phytoconstituents.</p><h3>Results</h3><p>Twelve compounds were isolated and identified as apigenin, luteolin, genkwanin, quercetin, hispidulin, luteolin-7-<i>O</i>-<i>β</i>- glucopyranoside, rutin, rosmarinic acid, caffeic acid, ferulic acid chlorogenic acid, and ellagic acid. The results of antioxidants indicated that SOEE exhibited the greatest efficacy as an antioxidant agent in the 1,1-Diphenyl-2-picrylhydrazyl assay with inhibitory concentration (IC₅₀) 23.21 ± 1.17 µg/ mL followed by 1864.71 and 1793.80 µM Trolox equivalent/mg extract for ferric ion reducing antioxidant power and 3-ethylbenzothiazoline-6-sulfonic acid (ABTS), respectively. SOEE showed notable cytotoxic activity against A549. Oral administration of SOEE at 69.4 and 173.5 mg/kg.bw afforded a noteworthy protective effect against HgCl₂-induced pulmonary fibrosis. Also, lung superoxide dismutase (SOD), catalase (CAT), and reduced glutathione (GSH) were increased by SOEE. That was in parallel with working to reduce the malondialdehyde (MDA), necrosis factor kappa (NF-κB), interleukin-1β (IL-1β), and cyclooxygenase-2 (COX-2) levels. The lung P53, Bcl-2, Bcl-XL, AKT, and vanin-1 gene expression was upregulated in SOEE-treated rats compared to HgCl₂-treated rats.</p><h3>Conclusion</h3><p>SOEE reduces acute lung injury and can be used to inhibit the proliferation and migration of lung fibrotic cells. These activities may be related to its high antioxidant activity, which could result from the phenolic constituents. The authors recommended using sage in the treatment of lung diseases to protect from lung fibrosis. Additional animal and human experiments are needed for future research.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"13 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-024-00498-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140817162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of cigarette and e-cigarette smoke on dental pulp stem cells proliferation capacity and differentiation [in vitro study]","authors":"Dalia Riad,&nbsp;Amani Nour Eldin,&nbsp;Mai Abdelhalim Hamouda","doi":"10.1186/s43088-024-00496-8","DOIUrl":"10.1186/s43088-024-00496-8","url":null,"abstract":"<div><h3>Background</h3><p>Mesenchymal stem cells (MSCs) have long been known for their ability to regenerate tissue. Cigarette smoking is one environmental risk factor that may impair the performance of MSCs. Electronic cigarettes have recently become a popular and widely accepted alternative to tobacco cigarettes due to their safety. The present study aims to analyze how smoke extracts of cigarette tobacco and electronic cigarettes affect the capability of dental pulp stem cell (DPSCs) proliferation and osteogenic differentiation. In this study, DPSCs were isolated from healthy impacted third molars of non-smokers, and two smoke extracts were made from tobacco powder and electronic cigarettes. Half maximal inhibitory concentration (IC50) was calculated at two time intervals (14 and 21 days), and its effect on the proliferation and osteogenic differentiation of the DPSCs was assessed.</p><h3>Results</h3><p>The proliferation rate with the calculated IC50 of both smoke extracts was reduced compared to control cells. After 21 days of osteogenic induction, significantly fewer calcium deposits were visible among cells exposed to both smoke extracts. In addition, the expression of alkaline phosphatase and RANKL proteins was significantly reduced in differentiated DPSCs subjected to both smoke extracts.</p><h3>Conclusions</h3><p>DPSCs exposed to both smoke extracts showed decreased cell viability and osteogenic differentiation potentiality compared to control cells. Smoking in any form has a detrimental effect on the proliferation and regenerative capacity of MSCs.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"13 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-024-00496-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140648096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Entropy generation analysis for magnetohydrodynamic flow of chemically reactive fluid due to an accelerated plate","authors":"T. N. Abdelhameed","doi":"10.1186/s43088-024-00497-7","DOIUrl":"10.1186/s43088-024-00497-7","url":null,"abstract":"<div><h3>Background</h3><p>The mixed convection flow of viscous fluid due to an oscillating plate is inspected. The external heating effects and chemical reaction assessment are predicted. Moreover, the flow applications of the entropy generation phenomenon are claimed.</p><h3>Results</h3><p>The dimensionless system is expressed in partial differential forms, which are analytically treated with the Laplace scheme. The physical aspects of the flow model are graphically observed. The optimized phenomenon is focused on flow parameters. The results for the Bejan number are also presented. The dynamic of heat transfer and entropy generation phenomenon is observed with applications of Bejan number.</p><h3>Conclusions</h3><p>It is claimed that an enhancement of entropy generation phenomenon is noticed due to heat and mass Grashof coefficients. The Bejan number declined due to mass Grashof number. Furthermore, the velocity profile boosted due to Grashof constant.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"13 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-024-00497-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140648098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cloning, heterologous expression and purification of the novel thermo-alkalistable cellulase from Geobacillus sp. TP-3 and its molecular characterisation","authors":"Meghna Arya,&nbsp;Garima Chauhan,&nbsp;Utsav Verma,&nbsp;Monica Sharma","doi":"10.1186/s43088-024-00495-9","DOIUrl":"10.1186/s43088-024-00495-9","url":null,"abstract":"<div><h3>Background</h3><p>Thermophilic cellulases are essential for effectively degrading cellulose, which is a significant part of lignocellulosic waste. In this study, we focused on a cellulase gene (~ 1.2 kb) obtained from <i>Geobacillus</i> sp. TP-3, a thermo-alkalophilic bacterium isolated from the hot springs of Tapovan (Uttarakhand, India). Cellulase gene (~ 1.2 kb) was amplified via PCR, cloned into pET-28a (+) vector, transferred to <i>Escherichia coli</i> DH5α cells and expressed in <i>Escherichia</i> coli BL21 (DE3). The recombinant cellulase (<i>rCel_TP</i>) was purified using Ni²⁺-NTA affinity chromatography.</p><h3>Results</h3><p>The purified <i>rCel_TP</i> enzyme exhibited optimal activity at 50 ºC and pH 8, displaying stability even after 3 h of incubation at 50 ºC. The molecular weight of the purified 6 × His-tagged <i>rCel_TP</i> was determined to be ~ 40.2 kDa. Under conditions of 50 ºC and pH 8, the kinetic parameters of the purified enzyme were determined, with K_m and V_max values of 116.78 mg/mL and 44.05 µmolmg⁻¹ min⁻¹, respectively. The activity of the <i>rCel_TP</i> cellulase was significantly improved by Hg²⁺, Cu²⁺ and Co²⁺. However, it was suppressed by dithiothreitol and β-mercaptoethanol. Ethylenediaminetetraacetic acid and solvents also had a slight inhibitory effect.</p><h3>Conclusion</h3><p>These results suggest the potential applications of the recombinant cellulase in biomass conversion processes for the production of fuels and other industrial operations. The study contributes valuable insights into the properties and applicability of cellulases derived from extremophilic microorganisms.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"13 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-024-00495-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140633642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}