Beni-Suef University Journal of Basic and Applied Sciences最新文献

{"title":"Relief of double-J stent-related symptoms: a comparison between mirabegron, tamsulosin and solifenacin","authors":"Akrm A. Elmarakbi,&nbsp;Osama M. Elsayed,&nbsp;Tamer R. Mohamed,&nbsp;Amr M. Lotfy","doi":"10.1186/s43088-024-00508-7","DOIUrl":"10.1186/s43088-024-00508-7","url":null,"abstract":"<div><h3>Background</h3><p>Inserting ureteral stents is a routine intervention that often results in problems. The cornerstone for treating stent-related symptoms is pharmacological therapy. This study was conducted to evaluate and to compare the effectiveness of mirabegron, tamsulosin, solifenacin and control in reducing double-J stent-related symptoms.</p><h3>Results</h3><p>Patients were evaluated preoperatively, one week after stent insertion and two weeks after the start of medications by the Ureteral Stent Symptom Questionnaire (USSQ), International Prostate Symptom Score (IPSS), overactive bladder questionnaire (OAB-q) and visual analogue pain scale (VAPS). Solifenacin and mirabegron groups had significantly lower sexual scores after the 1st and 2nd weeks post-operatively (PO) when compared with the control group. Mirabegron group had significantly lower sexual scores after 1st and 2nd weeks PO when compared with patients in tamsulosin and solifenacin groups. Patients in mirabegron group had significantly fewer additional problems after the 1st and 2nd weeks PO when compared with patients in the control and tamsulosin groups.</p><h3>Conclusions</h3><p>To sum up, mirabegron was found to be superior to solifenacin in lowering urinary symptoms scores, sexual performance scores and work performance scores at both first and second weeks post-operatively. Mirabegron is a good alternative choice for SRSs when tamsulosin or solifenacin is ineffective or not tolerated.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"13 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-024-00508-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141448065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A new mathematical technique and its Python program to assess wind potential","authors":"Shafiq Ur Rehman,&nbsp;Naeem Sadiq,&nbsp;Iqbal Tariq,&nbsp;Mahwish Mobeen Khan,&nbsp;Muhammad Mustaqeem Zahid,&nbsp;Ahmed Ali Rajput,&nbsp;Zaheer Uddin","doi":"10.1186/s43088-024-00510-z","DOIUrl":"10.1186/s43088-024-00510-z","url":null,"abstract":"<div><h3>Background</h3><p>A new approach based on the Newton–Gauss method is used to find the Weibull parameters.</p><h3>Results</h3><p>A Python program was developed to employ the Newton–Gauss method. It is implemented to find Weibull parameters and wind potential of Pakistan’s eight cities (Hyderabad, Khuzdar, Multan, Quetta, Bahawalpur, Islamabad, Lahore, and Peshawar). Wind speed data recorded at an interval of ten minutes for 2016 is used to implement a Python program to calculate wind potential. To compare the values of the parameters, five known methods, the empirical method, method of moments, energy pattern factor method, maximum likelihood method, and modified maximum likelihood method, were also used to model and determine the wind potential. The root mean square error, mean absolute error, coefficient of determination, and Akaike information criterion were calculated to compare values of wind parameters and average wind speed. The correlation between recorded and modeled Weibull pdf was almost 99% for each city.</p><h3>Conclusions</h3><p>The new method only caters to those wind speeds that contribute to the wind potential; therefore, the average value of the wind speed is the least in the case of the new method. The maximum wind potential was observed for Hyderabad.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"13 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-024-00510-z","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141444747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microsponges: a promising frontier for prolonged release-current perspectives and patents","authors":"N. Srinatha,&nbsp;Sowjanya Battu,&nbsp;B. A. Vishwanath","doi":"10.1186/s43088-024-00519-4","DOIUrl":"10.1186/s43088-024-00519-4","url":null,"abstract":"<div><h3>Background</h3><p>Microsponges are one of the advanced drug delivery systems that facilitates precise and controlled release of active ingredients that are suitable for topical and oral use. These porous microspheres are typically sized between 5 and 300 μm, offer benefits including controlled release, stability, and minimized side effects. Manufacturing techniques like quasi-emulsion solvent diffusion and liquid–liquid suspension polymerization are usually employed to prepare microsponges, although various challenges arise from the use of potentially hazardous organic solvents.</p><h3>Main body</h3><p>Microsponges possess distinct traits such as extended drug release, formulation flexibility, and high drug loading capacity. Entrapment of drugs requires considerations of solubility, stability, and miscibility, while evaluation methods encompass production yield and particle size analysis. Their applications range from dermatological to biopharmaceutical delivery, with diverse products utilizing this technology. Ongoing innovations about microsponges are evident in patents concerning medical dressings and hyaluronic acid delivery systems.</p><h3>Conclusion</h3><p>Microsponges present a promising avenue in drug delivery, despite many challenges. Current review addresses on limitations and diverse products highlighting commercial viability. Patent activity signifies continued interest, suggesting significant potential for enhancing patient care.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"13 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-024-00519-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141435575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Iron deficiency anemia in relation to pinch strength and hand dexterity in preschool children: a cross-sectional study","authors":"Fatma Essam Amer,&nbsp;Khaled Ahmed Mamdouh,&nbsp;Tamer Hasan Moustafa Hassan,&nbsp;Walaa A. Abd-El-Nabie,&nbsp;Hoda Eltalawy","doi":"10.1186/s43088-024-00518-5","DOIUrl":"10.1186/s43088-024-00518-5","url":null,"abstract":"<div><h3>Background</h3><p>Iron deficiency anemia (IDA) constitutes a considerable nutritional problem in Egyptian preschool children. Moreover, IDA limits physical activity performance and interferes with children's development. Accordingly, we aim to investigate the correlation between IDA and hand dexterity and pinch grip strength in preschool children.</p><h3>Results</h3><p>This observational cross-sectional study encompassed the participation of 87 children, comprising both genders, with an age range of 5–6 years. The cohort was initially stratified into three distinct groups: Group A comprised 36 non-anemic children, group B included 25 children with mild iron-deficiency anemia (IDA), and group C consisted of 26 children with moderate IDA. After group delineation, comprehensive laboratory analyses were performed on all participants to assess for IDA, involving a thorough examination of their complete blood picture, hemoglobin (Hb) levels, serum iron levels, and serum ferritin levels. The evaluative metrics employed in this study encompassed the Bruininks-Oseretsky Test of Motor Proficiency-Second Edition (BOT-2) and the Baseline Mechanical Pinch Gauge. These instruments were utilized to assess manual dexterity and pinch strength, specifically tip-to-tip and tripod strength. This investigation revealed a positive correlation between Hb, serum iron, and serum ferritin levels with both manual dexterity and pinch strength across all three groups.</p><h3>Conclusion</h3><p>This study highlights that IDA negatively affects hand dexterity and pinch grip strength in preschool children. Managing IDA early is crucial for improving their physical performance and overall development.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"13 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-024-00518-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141424777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functionalized chitosan-G-poly caprolactone vaccine delivery system fabricated to display antigen–antibody immune complexes of Mycobacterium tuberculosis elicits immune response in Ex-vivo model","authors":"Sam Ebenezer Rajadas,&nbsp;Vignesh Sounderrajan,&nbsp;Rajendran Amarnath Prabhakaran,&nbsp;Ragini Agrawal,&nbsp;Lavanya Jeyadoss,&nbsp;Mariappan Rajan,&nbsp;Krupakar Parthasarathy,&nbsp;Shakila Harshavardhan","doi":"10.1186/s43088-024-00520-x","DOIUrl":"10.1186/s43088-024-00520-x","url":null,"abstract":"<div><h3>Background</h3><p>Vaccine development against tuberculosis remains a global health imperative, necessitating robust immunogenicity and safety profiles. Nanoparticle-based delivery systems offer promising avenues to enhance vaccine efficacy while ensuring tolerability. This study explores the utilization of chitosan micelles as a delivery platform for immune complex vaccination against tuberculosis. Leveraging two key antigens of <i>Mycobacterium tuberculosis</i>, namely HspX and Mpt51, known for their relevance in latent tuberculosis and its co-infection with the human immunodeficiency virus, immune complexes were synthesized <i>in vitro</i> using antibodies raised against these antigens. The immune complexes were then conjugated onto chitosan micelles, characterized for their physicochemical properties, and evaluated for their biocompatibility and immunogenicity.</p><h3>Results</h3><p>Chitosan nanoparticles conjugated with either antigen or its immune complexes were synthesized as micelles and physicochemical characterizations confirm the formation of micelles without altering the polymer composition. These immune complex-conjugated chitosan micelles were found to be safe, exhibiting no significant hemolytic and cytotoxic activity even at a higher concentration of 400 µg/ml. Peripheral blood mononuclear cells upon stimulation with immune complex-conjugated chitosan micelles showed enhanced cellular uptake and one to two-fold increased expression of key immune markers—interferon gamma and CD-86.</p><h3>Conclusions</h3><p>These findings underscore the potential of chitosan nanoparticles as a versatile delivery platform for immune complex vaccination against tuberculosis. While limitations exist, such as including only two markers of immune modulation, this study lays a foundation for future investigations into immune complex vaccine potential in animal models. In conclusion, chitosan micelles carrying immune complexes of HspX and Mpt51 tuberculosis antigens exhibit promising immunogenicity, highlighting their potential as a platform for multi-antigenic vaccine components warranting further <i>in vivo</i> studies.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"13 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-024-00520-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141333511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization and utilization capabilities of industrial wastes for green bricks production","authors":"Medhat Sobhy El-Mahllawy,&nbsp;Sarah Akram Mohsen","doi":"10.1186/s43088-024-00517-6","DOIUrl":"10.1186/s43088-024-00517-6","url":null,"abstract":"<div><h3>Background</h3><p>The goal of this study is to develop a feasible and sustainable solution to manage the use of industrial wastes of ground granulated blast-furnace steel slag (GGBS) activated by cement kiln dust (CKD) and quicklime (QL). Using activated GGBS in the manufacture of stabilized green bricks is still uncommon in Egypt in such applications. Five clay-based mixtures, each with varying replacement ratios (5–10, wt.%) of CKD and QL, were studied. Laboratory tests were conducted on cylindrical specimens made from these mixtures, which were left to cure for periods of up to 60 days. The raw materials and lab-made specimens were analyzed using particle size analysis, differential thermal analysis, X-ray fluorescence, and X-ray diffraction techniques. The physical and mechanical properties of the cured specimens were also determined and evaluated according to standard specifications. Furthermore, the durability of the cured specimens was evaluated against collapsibility in water.</p><h3>ResuIts</h3><p>It has been observed that adding QL and CKD to the stabilized green specimens of different mixes can enhance their engineering properties with curing age increasing. This is due to the pozzolanic reaction, which fills the pore structure with calcium silicate hydrates and calcium aluminate hydrates gel. The ratio of QL and CKD used significantly affected the engineering properties of the specimens. The study found that using 20% GGBS and 5% QL led to an increase in compressive strength (266 kg/cm²) at the density of (2.15 g/cm³), while also water absorption was reduced (8%) to give superior results. When GGBS and CKD were combined, a higher content of CKD (10 wt.%) gave better results compared to (5 wt.%) CKD. Furthermore, the physical and mechanical properties of the tested specimens (MD 1, MD II, MD III and MD IV) met the acceptable limits of dry compressive strength (30–70 kg/cm²), water absorption (8–15%), and density (1.7–2 gm/cm³), as specified by the Egyptian standard specifications for buildings used compressed earth blocks.</p><h3>Conclusion</h3><p>The CKD and QL act as alkali activators for GGBS and can be utilized in masonry construction.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"13 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-024-00517-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141326429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Uncovering and evaluating coconut oil-loaded silica nanoemulsion as anti-viral, bacterial, and fungal: synthesis, fabrication, characterization, and biosafety profiles","authors":"Mohamed E. Elnosary,&nbsp;Hesham A. Aboelmagd,&nbsp;Ahmed R. Sofy,&nbsp;Ahmed A. Hmed,&nbsp;Ehab E. Refaey,&nbsp;Sayeda M. Ali,&nbsp;Mayssa Abdel Hady","doi":"10.1186/s43088-024-00513-w","DOIUrl":"10.1186/s43088-024-00513-w","url":null,"abstract":"<div><h3>Background</h3><p>Coconut oil, a natural component abundant in terpenoids, possesses various physiological functions. The global concern over the spread of viral infections and antimicrobial-resistant bacteria and fungi has highlighted the need for novel treatments. Coconut oil, with its known antimicrobial properties, presents an attractive candidate for combating these pathogens. This study aims to investigate the potential of coconut oil-loaded silica nanoemulsion (ON@SiO₂) as a novel therapeutic agent against viral, antimicrobial-resistant bacteria, and fungal pathogens.</p><h3>Results</h3><p>The study synthesized coconut oil-loaded silica nanoemulsion (ON@SiO₂) using an eco-friendly, cost-effective method with native coconut oil (CO). Characterization confirmed successful synthesis on the nanoscale with good distribution. Three nanoemulsion samples (ON-1@SiO₂, ON-2@SiO₂, and ON-3@SiO₂) were prepared, with average particle sizes of 193 nm, 200 nm, and 325 nm, respectively. Evaluation of cytotoxicity on Vero-E6 cell lines indicated safety of ON-0@SiO₂ and ON-3@SiO₂, with CC50 values of 97.5 mg/ml and 89.1 mg/ml, respectively. ON-3@SiO₂ demonstrated anti-Herpes I and II (HSV1 and HSV2) activity, with IC50 values of 1.9 mg/ml and 2.1 mg/ml, respectively. Additionally, ON-3@SiO₂ exhibited promising antibacterial activity against <i>E. coli, P. aeruginosa, S. aureus,</i> and <i>B. subtilis</i>, with MIC values of 25 mg/ml, 12.5 mg/ml, 25 mg/ml, and 3.12 mg/ml, respectively.</p><h3>Conclusions</h3><p>ON-3@SiO₂ showed potential antifungal activity against <i>C. albicans</i>, a unicellular fungus, with an MIC of 12.5 mg/ml. Overall, ON@SiO₂ possesses antiviral, antibacterial, and antifungal properties.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"13 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-024-00513-w","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141298357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitigating the escalating threat of infectious diseases outbreaks in tropical Africa: a perspective examination of challenges and strategies for future preparedness","authors":"Hakeem Kayode Hassan,&nbsp;Olaniyi Abideen Adigun,&nbsp;Emery Manirambona,&nbsp;Noah Olabode Olaleke,&nbsp;Micheal Sunday Abioye,&nbsp;Don Eliseo Lucero-Prisno III,&nbsp;Faith Ayobami Atewologun,&nbsp;Olalekan John Okesanya","doi":"10.1186/s43088-024-00511-y","DOIUrl":"10.1186/s43088-024-00511-y","url":null,"abstract":"<div><h3>Background</h3><p>The escalating threat of infectious disease outbreaks in Africa, particularly emerging and re-emerging diseases, necessitates urgent and comprehensive action. The frequency of these outbreaks demands a robust enhancement of notification and reporting systems to enable swift public health interventions.</p><h3>Main body of the abstract</h3><p>Tropical diseases such as malaria, COVID-19, typhoid fever, yellow fever, arboviruses, cholera, rabies, schistosomiasis, tuberculosis, black fungus, meningitis, evolving pathogens, and antimicrobial resistance pose significant health risks globally, especially in Sub-Saharan Africa. The region faces complexities in healthcare, including weak systems, inadequate surveillance, socioeconomic disparities, and other issues. Poor health literacy, traditional practices, and distrust hinder effective disease control and contribute to disease emergence in Sub-Saharan Africa. Continuous research and global collaboration are essential to address these public health concerns, especially given Africa's unique challenges. Disease surveillance emerges as a highly effective strategy, crucial in regions vulnerable to infectious diseases. Establishing and strengthening comprehensive surveillance and reporting systems at individual, regional, national, and international levels is crucial due to the unpredictable nature of borderless outbreaks and their significant impact on morbidity, mortality, and economic stability. National surveillance relies heavily on effective control mechanisms within local community areas, necessitating the active involvement of medical personnel. Successful systems depend on functional countries using collected data for timely warnings and localized interventions. Stakeholders, including governments, health authorities, and international organizations, must collaborate urgently to implement and sustain these vital systems, mitigating the devastating consequences of infectious disease outbreaks. Additionally, a holistic approach is crucial, involving prioritized local production of vaccines, medicines, and diagnostics through initiatives like the African Vaccine Producers Initiative. This approach emphasizes the need for domestic pharmaceutical production, intensified public awareness campaigns, and the training of the next generation of global health leaders, ensuring multidimensional strategies, political and diplomatic skills, and evidence-based assessments.</p><h3>Conclusion</h3><p>Collaboration among governments, international organizations, and educational institutions is essential for successful policy advocacy and implementation to strengthen health security and mitigate the continuous rise of infectious diseases on the continent.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"13 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-024-00511-y","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141294954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Network pharmacology, molecular docking study, and in vivo validation of the wound healing activity of the Red Sea soft coral Paralemnalia thyrsoides (Ehrenberg 1834) ethanolic extract and bioactive metabolites","authors":"Radwa Taher Mohie el-dien,&nbsp;Sherif A. Maher,&nbsp;Mohamed Hisham,&nbsp;Entesar Ali Saber,&nbsp;Amgad I. M. Khedr,&nbsp;Mostafa A. Fouad,&nbsp;Mohamed Salah Kamel,&nbsp;Basma Khalaf Mahmoud","doi":"10.1186/s43088-024-00512-x","DOIUrl":"10.1186/s43088-024-00512-x","url":null,"abstract":"<div><h3>Background</h3><p>Wounds are a major health issue on a global scale, putting a great deal of financial, commercial, and social strain on healthcare organizations, patients, and individuals. So, this study aims to investigate the <i>in vitro</i> antioxidant activity of <i>Paralemnalia thyrsoides</i> soft coral total ethanolic extract. Also, bio-guided <i>in vivo</i> wound healing validation enhanced by the evaluation of related gene expression of <i>Paralemnalia thyrsoides</i> total extract, derived fractions, and three known metabolites was done. Furthermore, utilizing network pharmacology, we identified ten hub target genes associated with wound healing, including <i>AKT1(RAC-alpha serine/threonine–protein kinase), IL6 (interleukin-6)</i><i>, </i><i>MAPK3</i><i> (mitogen-activated protein kinase 3), MMP9 (matrix metalloproteinase 9), and APP (amyloid P protein precursor).</i> We conducted molecular docking to assess how the three compounds interact with these hub genes and inflammatory cytokines <i>(IL-1β (interleukin-1 beta), TGF-β (transforming growth factor-beta), TNF-α (tumor necrosis factor-alpha),</i> and <i>NF-KB (nuclear factor-kappa B)</i> linked to wound healing.</p><h3>Results</h3><p><i>In vitro</i> antioxidant activity of the total ethanolic extract of <i>Paralemnalia thyrsoides</i> revealed potent scavenging activity against H₂O₂ with IC₅₀ of 178.62 μg/mL. Additionally, the bio-guided scheme of the <i>in vivo</i> wound healing assay leads to the most active fraction, petroleum ether, with a healing percentage of 85% ± 4. Several chromatographic procedures upon petroleum ether fraction led to the isolation of three known compounds with significant <i>in vivo</i> wound healing potential which are recognized as triacontan-1-ol (1), 24-methylcholesterol (2) 6α-acetyl-7α-acetate-1(10)-α-13-nornardosine [C₁₆H₂₄O₄] (3). Noteworthy downregulation in <i>Cox-2 (Cyclooxygenase-2), Cox-1 (Cyclooxygenase-1), IL-1β, TGF-β, TNF-α, NF-KB, and INF-ϒ (interferon-ϒ)</i> relative genes expression and upregulation in <i>TGF-β, and IL-10 (interleukin-10)</i> relative genes expression proved that compounds (3), (2), and (1) were, respectively, significant. The <i>in silico</i> study suggests that both C₁₆H₂₄O₄ and 24-methyl cholesterol have potential in wound healing, possibly involving the regulation of RAC-alpha serine/threonine-protein kinase (<i>AKT1</i>).</p><h3>Conclusion</h3><p>Our study highlights the antioxidant and wound-healing potential of <i>Paralemnalia thyrsoides</i> soft coral that can be attributed to its diverse chemical metabolites. The <i>in vivo</i> and <i>in silico</i> findings highlighted that <i>P. thyrsoides</i> can be an effective remedy for wound restoration with the need for extensive future detailed clinical studies to prove these results.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"13 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-024-00512-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141294953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tricyclic microwave-assisted synthesis of gold nanoparticles for biomedical applications: combatting multidrug-resistant bacteria and fungus","authors":"Sarah Al Azzam,&nbsp;Zabih Ullah,&nbsp;Sarfuddin Azmi,&nbsp;Mozaffarul Islam,&nbsp;Ishtiaque Ahmad,&nbsp;Mohd Kamil Hussain","doi":"10.1186/s43088-024-00514-9","DOIUrl":"10.1186/s43088-024-00514-9","url":null,"abstract":"<div><h3>Background</h3><p>Rising global mortality due to antibiotic-resistant pathogens necessitates novel antibacterial and antifungal agents. This study focuses on synthesizing gold nanoparticles (GNPs) via tricyclic microwave irradiation (TMI) to combat Multi-Drug-Resistant Bacteria and Fungus. The demand for sustainable synthesis methods has led to the exploration of TMI for GNP production.</p><h3>Results</h3><p>Characterization demonstrates consistent, uniform, and dispersed GNPs with trigonal and hexagonal shapes. GNPs sized 20–55 nm exhibit superior antibacterial and antifungal activity, particularly against drug-resistant Gram-positive bacteria. Notably, GNPs display consistent efficacy against drug-resistant fungus and demonstrate potential for broad-spectrum antimicrobial applications.</p><h3>Conclusion</h3><p>TMI-synthesized GNPs, characterized by their favorable physical properties and size-dependent efficacy, show promise as effective agents against drug-resistant pathogens. Their ability to combat Gram-positive bacteria, Gram-negative bacteria, and drug-resistant fungus positions them as valuable tools in biomedical sciences. By addressing the urgent need for novel antimicrobial agents, TMI-synthesized GNPs offer a sustainable solution to the escalating global health challenge of antibiotic resistance.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"13 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-024-00514-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141286749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}