Moustafa M. Morsy, R. G. Ahmed, Mohammed Abdel-Gabbar
{"title":"纳米姜黄素通过调节氧化应激、炎症和 COX-2/NF-κB/Nrf2 信号转导，改善咖啡因诱导的雄性 Wistar 大鼠脑部变化","authors":"Moustafa M. Morsy, R. G. Ahmed, Mohammed Abdel-Gabbar","doi":"10.1186/s43088-024-00526-5","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>This research aims to determine the probable protective effect of nano-curcumin (N-CUR) on caffeine (1,3,7-trimethylxanthine)-induced neurotoxicity in cerebral rats.</p><h3>Methods</h3><p>Twenty-four male Wistar rats were divided into three groups: control, caffeine (150 mg kg<sup>−1</sup>), and caffeine (150 mg kg<sup>−1</sup>) treated with N-CUR (300 mg kg<sup>−1</sup>). All treatments were administrated by gavage every day for a month.</p><h3>Results</h3><p>Administration of caffeine significantly elevated the levels of serum interleukins 6 (IL-6), tumor necrosis factor-alpha (TNF-α), vascular endothelial growth factor (VEGF), and cyclooxygenase2 (COX-2). Also, there was a significant increase in levels of cerebral malondialdehyde (MDA), significantly diminished glutathione (GSH), and superoxide dismutase (SOD) activity. Caffeine administration significantly downregulated the gene expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and upregulated the expression of nuclear factor kappa-light-chain enhancer of activated B cells (NF-κB). Administration of N-CUR caused a significant amelioration in TNF-α and IL-6 levels and a significant rise in SOD activity, while it caused a significant downregulation in NF-κB mRNA expression. Additionally, N-CUR has exerted insignificant amelioration of COX-2 and MDA contents and Nrf2 mRNA expression compared to the caffeine-treated group.</p><h3>Conclusion</h3><p>N-CUR may have a mild to moderate ameliorative effect on caffeine-induced apoptosis, oxidative stress, and inflammatory response in the cerebrum.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"13 1","pages":""},"PeriodicalIF":2.5000,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-024-00526-5","citationCount":"0","resultStr":"{\"title\":\"Nano-Curcumin improves caffeine-induced cerebral alterations in male Wistar rats by modifying oxidative stress, inflammation, and COX-2/NF-κB/Nrf2 signaling\",\"authors\":\"Moustafa M. Morsy, R. G. Ahmed, Mohammed Abdel-Gabbar\",\"doi\":\"10.1186/s43088-024-00526-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>This research aims to determine the probable protective effect of nano-curcumin (N-CUR) on caffeine (1,3,7-trimethylxanthine)-induced neurotoxicity in cerebral rats.</p><h3>Methods</h3><p>Twenty-four male Wistar rats were divided into three groups: control, caffeine (150 mg kg<sup>−1</sup>), and caffeine (150 mg kg<sup>−1</sup>) treated with N-CUR (300 mg kg<sup>−1</sup>). All treatments were administrated by gavage every day for a month.</p><h3>Results</h3><p>Administration of caffeine significantly elevated the levels of serum interleukins 6 (IL-6), tumor necrosis factor-alpha (TNF-α), vascular endothelial growth factor (VEGF), and cyclooxygenase2 (COX-2). Also, there was a significant increase in levels of cerebral malondialdehyde (MDA), significantly diminished glutathione (GSH), and superoxide dismutase (SOD) activity. Caffeine administration significantly downregulated the gene expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and upregulated the expression of nuclear factor kappa-light-chain enhancer of activated B cells (NF-κB). Administration of N-CUR caused a significant amelioration in TNF-α and IL-6 levels and a significant rise in SOD activity, while it caused a significant downregulation in NF-κB mRNA expression. Additionally, N-CUR has exerted insignificant amelioration of COX-2 and MDA contents and Nrf2 mRNA expression compared to the caffeine-treated group.</p><h3>Conclusion</h3><p>N-CUR may have a mild to moderate ameliorative effect on caffeine-induced apoptosis, oxidative stress, and inflammatory response in the cerebrum.</p></div>\",\"PeriodicalId\":481,\"journal\":{\"name\":\"Beni-Suef University Journal of Basic and Applied Sciences\",\"volume\":\"13 1\",\"pages\":\"\"},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2024-09-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-024-00526-5\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Beni-Suef University Journal of Basic and Applied Sciences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://link.springer.com/article/10.1186/s43088-024-00526-5\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MULTIDISCIPLINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Beni-Suef University Journal of Basic and Applied Sciences","FirstCategoryId":"1085","ListUrlMain":"https://link.springer.com/article/10.1186/s43088-024-00526-5","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}
Nano-Curcumin improves caffeine-induced cerebral alterations in male Wistar rats by modifying oxidative stress, inflammation, and COX-2/NF-κB/Nrf2 signaling
Background
This research aims to determine the probable protective effect of nano-curcumin (N-CUR) on caffeine (1,3,7-trimethylxanthine)-induced neurotoxicity in cerebral rats.
Methods
Twenty-four male Wistar rats were divided into three groups: control, caffeine (150 mg kg−1), and caffeine (150 mg kg−1) treated with N-CUR (300 mg kg−1). All treatments were administrated by gavage every day for a month.
Results
Administration of caffeine significantly elevated the levels of serum interleukins 6 (IL-6), tumor necrosis factor-alpha (TNF-α), vascular endothelial growth factor (VEGF), and cyclooxygenase2 (COX-2). Also, there was a significant increase in levels of cerebral malondialdehyde (MDA), significantly diminished glutathione (GSH), and superoxide dismutase (SOD) activity. Caffeine administration significantly downregulated the gene expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and upregulated the expression of nuclear factor kappa-light-chain enhancer of activated B cells (NF-κB). Administration of N-CUR caused a significant amelioration in TNF-α and IL-6 levels and a significant rise in SOD activity, while it caused a significant downregulation in NF-κB mRNA expression. Additionally, N-CUR has exerted insignificant amelioration of COX-2 and MDA contents and Nrf2 mRNA expression compared to the caffeine-treated group.
Conclusion
N-CUR may have a mild to moderate ameliorative effect on caffeine-induced apoptosis, oxidative stress, and inflammatory response in the cerebrum.
期刊介绍：
Beni-Suef University Journal of Basic and Applied Sciences (BJBAS) is a peer-reviewed, open-access journal. This journal welcomes submissions of original research, literature reviews, and editorials in its respected fields of fundamental science, applied science (with a particular focus on the fields of applied nanotechnology and biotechnology), medical sciences, pharmaceutical sciences, and engineering. The multidisciplinary aspects of the journal encourage global collaboration between researchers in multiple fields and provide cross-disciplinary dissemination of findings.
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