Beni-Suef University Journal of Basic and Applied Sciences最新文献

{"title":"Novel transferosome formulation of Vemurafenib for skin cancer management: biocompatible and scalable strategies","authors":"Tariq M. Aljarba,&nbsp;Aftab Alam,&nbsp;Ahmed Farag El Kirdasy","doi":"10.1186/s43088-025-00671-5","DOIUrl":"10.1186/s43088-025-00671-5","url":null,"abstract":"<div><p>Due to challenges such as poor aqueous solubility and compromised oral bioavailability, delivering Vemurafenib via a topical route using a scalable and biocompatible carrier-based hydrogel. This study aims to develop and characterize Vemurafenib-loaded transferosomes for the management of skin cancer. A Vemurafenib-loaded transferosomal gel was developed and thoroughly analyzed using various techniques, including transmission electron microscopy, ultraviolet spectroscopy, dermatokinetic parameters, entrapment efficiency, stability assessment, in vitro release study, vesicle elasticity examination, and antioxidant assays. The in vitro release of formulations was analyzed using four models: Korsmeyer, Higuchi, first-order, and zero-order models. The transferosomes exhibited a typical size of 105 nm, with a zeta size of 106.31 nm and a polydispersity index of 0.2417. Among the models investigated for in vitro release analysis, the Higuchi model was found to be the most suitable for the transferosome formulation. Compared to the standard formulation, the Vemurafenib-loaded transferosomal gel achieved a significantly higher concentration of 140.45 µg/ml on the skin epidermis within just 1.5 h. Additionally, in two hours, the Vemurafenib-loaded transferosomal gel resulted in a greater concentration of 118.52 µg/ml in the skin dermis, surpassing the usual formulation. Furthermore, the group receiving twice-daily administration of Vemurafenib-loaded transferosomal gel exhibited minimal hyperkeratosis compared to other treatment groups. The (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) (MTT) assay showed a higher A-431 cell lines inhibition under vemurafenib Hydrogel formulation, i.e., 78.28%. This study offers compelling evidence for the effectiveness of the Vemurafenib transferosomal gel, demonstrating its enhanced skin absorption. The formulation shows considerable promise for further research and potential clinical application in skin cancer treatment.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-025-00671-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144868890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Huperzine A: a natural acetylcholinesterase inhibitor with multifunctional neuroprotective effects","authors":"Suvaiv,&nbsp;Kuldeep Singh,&nbsp;Syed Misbahul Hasan,&nbsp;Arun Kumar,&nbsp;Abdullah khan,&nbsp;Mo. Shahanawaz,&nbsp;Syed Mehdi Hasan Zaidi,&nbsp;Kausal Verma","doi":"10.1186/s43088-025-00675-1","DOIUrl":"10.1186/s43088-025-00675-1","url":null,"abstract":"<div><h3>Background</h3><p>Huperzine A, a naturally derived compound, has garnered interest for its capacity to inhibit cholinesterase with multifaceted neuroprotective effects and is obtained from Huperzia serrata (Chinese club moss plant). This review highlights its pharmacological potential in the treatment of neurodegenerative disorders, particularly Alzheimer’s disease (AD). Huperzine A was used in various neurological conditions in traditional Chinese treatments.</p><h3>Findings</h3><p>Acetylcholinesterase is responsible for the breakdown of acetylcholine, a neurotransmitter critical for cognitive functions such as memory, learning, and attention. Huperzine A exhibits neuroprotective effects by preserving acetylcholine levels, and also offers antioxidant and anti-inflammatory benefits. These pharmacological actions suggest a potential role in modifying disease progression in AD and vascular dementia (VD). Although preclinical and clinical studies have demonstrated promising cognitive benefits, discrepancies in outcomes still exist. While considered safe at therapeutic dosages, excessive intake may lead to adverse effects, e.g., nausea, diarrhea, and muscle cramps.</p><h3>Conclusion</h3><p>Research on its effectiveness in various neurological conditions is ongoing, and its use should be approached with caution and professional guidance.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-025-00675-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144858581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights into the regulatory role of the lncRNA H19 and miRNA-152 and their cross-talk with their target genes in glioblastoma","authors":"M. A. Abdelsamed,&nbsp;N. A. Hasona,&nbsp;A. Lotfy,&nbsp;H. Y. Abdallah","doi":"10.1186/s43088-025-00670-6","DOIUrl":"10.1186/s43088-025-00670-6","url":null,"abstract":"<div><h3>Background</h3><p>Glioblastoma is a highly aggressive subtype of glioma. The alteration of non-coding RNA (lncRNA H19 and microRNA-152) in glioblastoma tissues promotes cell proliferation, migration, and invasion, while the exact relationship with glioblastoma is still uncertain with their genes (PTEN, KRAS, and NDRG1). This study aimed to identify new potential biomarkers for early diagnosis and novel therapeutic targets.</p><h3>Methods</h3><p>In a descriptive cross-sectional study, we employed quantitative real-time PCR for expression of lncRNA H19, miRNA-152, and their target genes in 84 glioblastoma specimens compared to 35 control samples (low-grade glioma, astrocytic astrocytoma, normal brain tissues). Additionally, for differential expression profile, predictive significance, and survival analysis, receiver operating characteristic analysis and Kaplan–Meier survival plot were used.</p><h3>Results</h3><p>The expression levels of lncRNA H19 and miR-152 were significantly altered in glioblastoma patients compared to those with low-grade glioma and normal brain tissues. Moreover, KRAS and NDRG1 showed significant upregulation in glioblastoma. It was demonstrated that lncRNA H19 has diagnostic values with AUC &gt; 0.7 that differentiated glioblastoma from non-cancerous lesions and low-grade glioma. Nevertheless, KRAS and NDRG1 with AUC &gt; 0.9 and &gt; 0.8, respectively, distinguished between glioblastoma and all other comparative groups including non-cancerous lesions, low-grade glioma, and astrocytic astrocytoma. Furthermore, poor overall survival was observed with a median survival rate of 15 months.</p><h3>Conclusions</h3><p>The long non-coding RNA H19, along with KRAS and NDRG1, has shown promise as biomarkers for differentiating between glioblastoma, lower-grade glioma, and non-malignant lesions.</p><h3>Key points</h3><ul>\u0000 <li>\u0000 <p>The expression levels of the lncRNA H19 and miR-152 were significantly altered in Glioblastoma patients compared to those with Low Grade Glioma and normal brain tissues.</p>\u0000 </li>\u0000 </ul><ul>\u0000 <li>\u0000 <p>The lncRNA H19, along with the genes KRAS and NDRG1, have shown promise as biomarkers for differentiating between Glioblastoma, Low Grade Glioma, and normal brain tissues.</p>\u0000 </li>\u0000 </ul></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-025-00670-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144832089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical analysis and evaluation of inhibitory potential of Ricinus communis leaf extract against novel Bacillus pathogens","authors":"Diptimayee Acharya,&nbsp;Puspanjali Parida,&nbsp;Himansu Sekhara Mohapatra,&nbsp;Subhasmita Mallik,&nbsp;Jatindra Nath Mohanty,&nbsp;Santi Lata Sahoo","doi":"10.1186/s43088-025-00666-2","DOIUrl":"10.1186/s43088-025-00666-2","url":null,"abstract":"<div><p>Mastitis is a common and complex disease, mainly caused by aureus and non-aureus species, and <i>Enterobacteriaceae</i>. Recently, a new and serious group of Gram-positive bacteria, the sporulating bacilli, has emerged as a mastitis pathogen. The study aimed to identify and characterize novel Bacillus strains (HSM85, SLS01, and DA03) associated with bovine mastitis and to evaluate the antimicrobial efficacy of <i>Ricinus communis</i> (castor) leaf extract against these pathogens. In methodology, Bacillus strains were isolated from milk samples of mastitis-infected cows, and the antimicrobial activity of different solvent-based leaf extract fractions was assessed using MIC, MBC, killing and biofilm inhibition assays, and GC–MS analysis to identify bioactive compounds. In result we found, the chloroform (non-polar) fraction showed the highest antimicrobial activity, followed by the ethanol (polar) fraction. MIC and MBC values ranged from 25 to 200 and 25–400 µg/ml, respectively. Bacterial growth was significantly inhibited at ½ MIC, while control cells entered log phase within 4 h. Assays (MIC, MBC, MBC/MIC ≤ 4, killing, biofilm inhibition, and nucleic acid leakage) confirmed the bactericidal potential of <i>Ricinus communis</i> leaf extract against novel <i>Bacillus</i> strains. GC–MS analysis revealed 48–53 peaks across solvent extracts, identifying dominant compounds like phytol, phytyl palmitate, and tetracontane, with some solvent-specific presence. <i>Ricinus communis</i> leaf extract showed strong bactericidal activity against emerging <i>Bacillus</i> strains causing bovine mastitis, with the chloroform fraction showing the highest efficacy. Its bioactive compounds and multi-target properties suggest its potential as a natural alternative for managing mastitis in dairy cattle.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-025-00666-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144810774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ginger oil microemulsion improves reproductive performance of male albino rats: sexual behavior, gross testicular measurements, spermatogenesis, and expression of sperm capacitation genes","authors":"Enas K. Aziz,&nbsp;Nagwa I. Sherabia,&nbsp;Doaa A. Mansour,&nbsp;Mona A. Nasr,&nbsp;Eman Elhusseiny,&nbsp;Afaf A. Kishta,&nbsp;Ahmed E. Elweza,&nbsp;Hend Mohmed Abdel-Bar,&nbsp;Reda M. Korany,&nbsp;Basma A. Elshafey","doi":"10.1186/s43088-025-00654-6","DOIUrl":"10.1186/s43088-025-00654-6","url":null,"abstract":"<div><h3>Background</h3><p>The effectiveness of nanoparticles in enhancing male sexual performance is a topic of great attention these days. The research goal was to evaluate the potency of ginger oil (G-Oil) and ginger oil microemulsion (G-ME) on male rat fertility and reproductive performance. Thirty-five male albino rats were split up into five groups and delivered oral gavage three times a week for eight weeks: control (1 ml /kg bw distilled water), corn oil (1 ml /kg bw corn oil), ginger oil (75 mg/kg bw ginger oil in corn oil as a vehicle), ginger oil microemulsion at two doses (75 and 37.5 mg/kg bw, respectively). Assessments of sexual behavior, sperm quality, relative testes weight, serum sex hormones, testicular oxidant/antioxidant status, sperm capacitation markers, and histomorphology and immunohistochemical changes in both testicles and epididymis were utilized.</p><h3>Results</h3><p>The results revealed that G-ME administration, especially at a low dose (37.5 mg/kg bw), significantly <i>(p</i> &lt; <i>0.05)</i> improved sexual behavior, relative testes weight, serum testosterone, and activating antioxidant enzyme Superoxide dismutase (SOD) along with reducing malondialdehyde (MDA) content in testicular tissues, indicating its antioxidant activity. Moreover, G-ME had significantly <i>(p</i> &lt; <i>0.001)</i> higher expression of <i>SPACA1</i> and <i>CD59b</i> genes, accompanied by improvements in sperm motility, count, and viability compared to the control group. Additionally, G-ME 37.5 mg/kg bw showed remarkable histological enhancements as spermatogenic cells and spermatozoa increased in seminiferous tubules and epididymis tubular lumen. Also, a stimulatory effect was detected on proliferating cells with increasing PCNA immunodepression in testicular and epididymal tissues.</p><h3>Conclusions</h3><p>Finally, the results obtained revealed the superiority of G-ME at a low dose of 37.5 mg/kg above the traditional G-Oil due to improvements in its physical properties, which resulted in increased ginger bioavailability and improved different rat fertility indices.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-025-00654-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145163655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Naringin from Citrus maxima peel: a potential therapeutic agent for breast cancer via PKM2 inhibition","authors":"Flama Monteiro,&nbsp;Vijith Vittal Shetty,&nbsp;Ranjitha Acharaya,&nbsp;Sriram Naresh,&nbsp;Manne Munikumar,&nbsp;Shilpa S Shetty,&nbsp;Pradeep Nataranjan,&nbsp;Suchetha Kumari N","doi":"10.1186/s43088-025-00668-0","DOIUrl":"10.1186/s43088-025-00668-0","url":null,"abstract":"<div><h3>Introduction</h3><p>An extensive array of medicinal plants has undergone investigation, underscoring the imperative for the continued screening of natural inhibitors with the potential to target cancer metabolism. The current research endeavor was directed toward evaluating the chemotherapeutic efficacy of peel extracts of <i>Citrus maxima</i> and its constituent flavonoid, Naringin (NA), in the context of breast cancer, specifically targeting the pyruvate kinase isozyme M2 (PKM2).</p><h3>Materials and methods</h3><p>Extracts from the peel of <i>Citrus maxima</i> were prepared and analyzed using liquid chromatography–mass spectrometry (LC–MS) to detect the presence of the bioactive compound, NA. The potential anti-proliferative effects of these peel extracts of <i>Citrus maxima</i> and NA were examined against human breast cancer cell lines utilizing an MTT assay. To investigate the distribution of the cell cycle, cell cycle analysis was conducted. The induction of apoptosis was ascertained using Annexin V-FITC through flow cytometry. The protein expression of PKM2 was analyzed using Western blotting. Molecular docking and dynamics simulations analysis were employed.</p><h3>Results</h3><p>Liquid chromatography–mass spectrometry (LC–MS) analysis confirmed the existence of NA within the extracts of <i>Citrus maxima</i>. Both the crude extracts and NA demonstrated a dose-dependent inhibition of breast cancer cell proliferation. Our findings indicate that these crude extracts and NA instigate both early and late apoptosis, in addition to causing cell cycle arrest in the G2/M phase. Immunoblotting studies further revealed that the expression of PKM2 protein was suppressed by both the crude extracts and NA. Computational analysis demonstrated stable binding affinity with Ser77, His78, and Lys207 of PKM2.</p><h3>Conclusion</h3><p>This investigation unveils the presence of NA within <i>Citrus maxima</i> extracts, exhibiting robust affinity for PKM2 via molecular docking and dynamics simulations. Extracts and NA dose-dependently inhibit breast cancer cell proliferation. Notably, PKM2 regulates cancer cell glycolysis, promising intricate therapeutic prospects for breast cancer.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-025-00668-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145162052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ethyl acetate extract from red coralline macroalgae Mastophora rosea, rich in polyphenolic catechins, induces apoptosis and inhibits metastasis in melanoma cell line","authors":"Eka Sunarwidhi Prasedya,&nbsp;Putu Bella Aprillia Saraswati,&nbsp;Ni Wayan Riyani Martyasari,&nbsp;Andri Frediansyah,&nbsp;Munawar Khalil,&nbsp;Sri Widyastuti","doi":"10.1186/s43088-025-00665-3","DOIUrl":"10.1186/s43088-025-00665-3","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Background&lt;/h3&gt;&lt;p&gt;The rise in global temperatures and subsequent increase in extreme heatwaves is implicated to have a significant contribution to the growing incidence of various skin diseases, particularly melanoma skin cancer. The existing therapeutic approaches for skin cancer such as chemotherapy induce significant adverse effects. This highlights the need for further development of novel medicinal substances from natural sources which has less side effects implications. Macroalgae, often known as seaweeds, are recognized for their diverse array of bioactive compounds, which have numerous potential applications. The red coralline macroalgae or seaweed &lt;i&gt;Mastophora&lt;/i&gt; &lt;i&gt;rosea&lt;/i&gt; is abundant in the tropical Indo-Pacific region, including Indonesia. However, little is known regarding the phytochemical profile and potent bioactivity of this unique seaweed. The objective of this study is to assess the phytochemical profile, antioxidant and cytotoxic activity of &lt;i&gt;M.&lt;/i&gt; &lt;i&gt;rosea&lt;/i&gt; in melanoma cell line.&lt;/p&gt;&lt;h3&gt;Methodology&lt;/h3&gt;&lt;p&gt;The dried macroalgae &lt;i&gt;M.&lt;/i&gt; &lt;i&gt;rosea&lt;/i&gt; sample was extracted using various solvents with differing polarities: ethanol, ethyl acetate (EA), and n-hexane. Subsequent biochemical evaluations included total phenolic content and antioxidant capability. Additionally, cytotoxic experiments were conducted using the mammalian melanoma cell line SK-MEL-2.&lt;/p&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;p&gt;The ANOVA results revealed that the EA solvent was the most efficient in producing extracts with high total phenolic content (368.81 ± 3.16 mg GAE/g). This high total phenolic content is accompanied by potent antioxidant capacity (IC&lt;sub&gt;50&lt;/sub&gt; = 99.52 ± 19.58 µg/mL). In addition, &lt;i&gt;M.&lt;/i&gt; &lt;i&gt;rosea&lt;/i&gt; EA extract is attributed with the presence of various polyphenolic catechins, such as gallic acid, gallocatechin, epigallocatechin (EGC), catechin, epicatechin, epigallocatechingallate (EGCG), and epicatechingallate (ECG). Furthermore, &lt;i&gt;M.&lt;/i&gt; &lt;i&gt;rosea&lt;/i&gt; EA shows strong cytotoxic activity against the melanoma cell line SK-MEL-2 (IC&lt;sub&gt;50&lt;/sub&gt; = 37.10 ± 0.85 µg/mL). In addition to the melanoma cell line, the &lt;i&gt;M.&lt;/i&gt; &lt;i&gt;rosea&lt;/i&gt; EA extract shows similar cytotoxicity in epithelial HeLa cells (IC&lt;sub&gt;50&lt;/sub&gt; = 34.32 ± 1.19 µg/mL) and low cytotoxicity in normal human dermal fibroblast cells (IC&lt;sub&gt;50&lt;/sub&gt; &gt; 200 µg/mL).&lt;/p&gt;&lt;h3&gt;Conclusion&lt;/h3&gt;&lt;p&gt;The current results show that &lt;i&gt;M.&lt;/i&gt; &lt;i&gt;rosea&lt;/i&gt; could induce apoptosis and also suppress metastasis activity in SK-MEL-2 cells. Further characterization and isolation of the possible bioactive compounds are necessary to better comprehend its mechanism of action against melanoma. Nevertheless, this study indicates that more study needed to be done on various macroalgae regarding their phenolic components that may be beneficial for the development of novel therapeutic agents for cancer treatment.&lt;/p&gt;&lt;h3&gt;Graphical abstract&lt;/h3&gt;&lt;div&gt;&lt;figure&gt;&lt;div&gt;&lt;div&gt;&lt;picture&gt;&lt;source&gt;&lt;img&gt;&lt;/source&gt;&lt;/pict","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-025-00665-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145161261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bag Valve Mask with Ventilation Indicator (BVM-VI) prototype for ventilation rhythm in Basic Life Support","authors":"Pratama Yulianto,&nbsp;Endang Triyanto,&nbsp;Ridlwan Kamaluddin,&nbsp;Adhe Akbar Azanni","doi":"10.1186/s43088-025-00664-4","DOIUrl":"10.1186/s43088-025-00664-4","url":null,"abstract":"<div><h3>Background</h3><p>Bag valve mask (BVM) are a practical choice for emergency medical personnel to treat out-of-hospital cardiac arrest (OHCA). However, the use of currently available conventional BVM still often results in overventilation in cases of cardiac arrest or respiratory failure. Globally, 79% of hyperventilation events occur during Basic Life Support (BLS) with BVM. This study aims to produce a prototype BVM-VI.</p><h3>Method</h3><p>The prototyping method of Research and Development design was chosen as the primary method, followed by black box testing with clinical, practical, and academic experts, and ended with limited scale testing with 10 users.</p><h3>Result</h3><p>BVM-VI is the result of combining conventional BVM with a portable indicator light module to signal ventilation actions. Expert assessment using the black box test method with CVI analysis resulted in a content validity score of 100%. A limited study conducted by nursing students using a survey method showed reliability with an Intraclass Correlation Coefficient (ICC) of 0.769.</p><h3>Conclusion</h3><p>BVM-VI became the initial prototype for developing a ventilation aid with breathing rhythm adjustment in BLS.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-025-00664-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145160810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research progress on the application of near-infrared spectroscopy in liquid food quality testing","authors":"Wenliang Qi,&nbsp;Qingqing Jiang,&nbsp; Erihemu","doi":"10.1186/s43088-025-00660-8","DOIUrl":"10.1186/s43088-025-00660-8","url":null,"abstract":"<div><p>Near-infrared spectroscopy is a spectral analysis technique that involves electromagnetic radiation at wavelengths between the visible spectrum and the mid-infrared spectrum. The technique can realize non-destructive testing of samples and is particularly suitable for rapid analysis in the field and online quality monitoring. This technique utilizes the absorption of near-infrared light by hydrogen-containing groups within a sample, enabling the extraction of specific information regarding the characteristics of these hydrogen-containing groups within the analyzed organic molecules. The application of chemometrics in near-infrared spectral analysis reduces the data set size, excludes noise interference and improves the efficiency of multivariate correction. The finite variable model construction reduces the random errors and improves the accuracy of the prediction results. The present document synthesizes the application of near-infrared spectroscopy and chemometrics in the context of liquid food safety detection. It provides a comprehensive overview of the state-of-the-art research in this field, emphasizing the role of chemometrics analysis in evaluating the quality and safety of liquid foods. Additionally, it delves into the merits and limitations of this technological approach, offering insight into its potential for future advancement and expansion. </p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-025-00660-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145171037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation of Aloe vera extract-loaded chitosan nanoparticles for the controlled delivery of extract phytochemicals in carbon tetrachloride-induced liver injury rat model","authors":"Omar Ashraf,&nbsp;Alyaa Farid,&nbsp;Gehan Safwat","doi":"10.1186/s43088-025-00663-5","DOIUrl":"10.1186/s43088-025-00663-5","url":null,"abstract":"<div><h3>Background</h3><p>A significant contributing factor to liver damage is drug consumption. Phytochemicals of <i>Aloe vera</i> extract are effective against a variety of diseases. Consequently, this study aimed to create chitosan nanoparticles (chi NPs) that were loaded with <i>Aloe vera</i> extract (ALV extract) to increase the delivery of the extract's bioactive materials. ALV extract, chi NPs, and <i>Aloe vera</i> extract-loaded chitosan nanoparticles (ALV-chi NPs) underwent anti-oxidant, anti-inflammatory, and cytotoxicity tests. The preventive and therapeutic effects of ALV-chi NPs against carbon tetrachloride (CCl4)-induced liver injury were assessed using a male Sprague Dawley rat model.</p><h3>Results</h3><p>Our findings demonstrated that the synthesis of ALV-chi NPs was a promising option for combining the therapeutic benefits of both ALV extract (included in its phytochemicals) and chi NPs. ALV-chi NPs have a uniformly distributed smooth shape with a size of 48.3 ± 2.97 nm, similar to the hydrodynamic size (50.9 ± 0.07 nm), and a surface charge of 38.16 mV. At a 1000 μg/mL concentration, ALV-chi NPs showed high DPPH scavenging % and a high hemolysis inhibition %. At 75 μg/mL, ALV-chi NPs showed lower PTT (63.7 s) than ALV extract (71.2 s). The phenolic components and flavonoids in the extract were released under controlled conditions throughout time, and their bioavailability was enhanced by loading the extract on chi NPs.</p><h3>Conclusions</h3><p>Among all tested formulations, ALV-chi NPs demonstrated superior efficacy, showing 95.4% DPPH scavenging (vs. 91.8% for free extract), 94.1% hemolysis inhibition (comparable to indomethacin), and optimal hepatoprotection in CCl4-induced liver injury. ALV-chi NPs ameliorated the raised levels of liver function parameters, pro-inflammatory cytokines, and intracellular apoptotic proteins.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-025-00663-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145171730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}