Beni-Suef University Journal of Basic and Applied Sciences最新文献

{"title":"Naringin from Citrus maxima peel: a potential therapeutic agent for breast cancer via PKM2 inhibition","authors":"Flama Monteiro,&nbsp;Vijith Vittal Shetty,&nbsp;Ranjitha Acharaya,&nbsp;Sriram Naresh,&nbsp;Manne Munikumar,&nbsp;Shilpa S Shetty,&nbsp;Pradeep Nataranjan,&nbsp;Suchetha Kumari N","doi":"10.1186/s43088-025-00668-0","DOIUrl":"10.1186/s43088-025-00668-0","url":null,"abstract":"<div><h3>Introduction</h3><p>An extensive array of medicinal plants has undergone investigation, underscoring the imperative for the continued screening of natural inhibitors with the potential to target cancer metabolism. The current research endeavor was directed toward evaluating the chemotherapeutic efficacy of peel extracts of <i>Citrus maxima</i> and its constituent flavonoid, Naringin (NA), in the context of breast cancer, specifically targeting the pyruvate kinase isozyme M2 (PKM2).</p><h3>Materials and methods</h3><p>Extracts from the peel of <i>Citrus maxima</i> were prepared and analyzed using liquid chromatography–mass spectrometry (LC–MS) to detect the presence of the bioactive compound, NA. The potential anti-proliferative effects of these peel extracts of <i>Citrus maxima</i> and NA were examined against human breast cancer cell lines utilizing an MTT assay. To investigate the distribution of the cell cycle, cell cycle analysis was conducted. The induction of apoptosis was ascertained using Annexin V-FITC through flow cytometry. The protein expression of PKM2 was analyzed using Western blotting. Molecular docking and dynamics simulations analysis were employed.</p><h3>Results</h3><p>Liquid chromatography–mass spectrometry (LC–MS) analysis confirmed the existence of NA within the extracts of <i>Citrus maxima</i>. Both the crude extracts and NA demonstrated a dose-dependent inhibition of breast cancer cell proliferation. Our findings indicate that these crude extracts and NA instigate both early and late apoptosis, in addition to causing cell cycle arrest in the G2/M phase. Immunoblotting studies further revealed that the expression of PKM2 protein was suppressed by both the crude extracts and NA. Computational analysis demonstrated stable binding affinity with Ser77, His78, and Lys207 of PKM2.</p><h3>Conclusion</h3><p>This investigation unveils the presence of NA within <i>Citrus maxima</i> extracts, exhibiting robust affinity for PKM2 via molecular docking and dynamics simulations. Extracts and NA dose-dependently inhibit breast cancer cell proliferation. Notably, PKM2 regulates cancer cell glycolysis, promising intricate therapeutic prospects for breast cancer.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-025-00668-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145162052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ethyl acetate extract from red coralline macroalgae Mastophora rosea, rich in polyphenolic catechins, induces apoptosis and inhibits metastasis in melanoma cell line","authors":"Eka Sunarwidhi Prasedya,&nbsp;Putu Bella Aprillia Saraswati,&nbsp;Ni Wayan Riyani Martyasari,&nbsp;Andri Frediansyah,&nbsp;Munawar Khalil,&nbsp;Sri Widyastuti","doi":"10.1186/s43088-025-00665-3","DOIUrl":"10.1186/s43088-025-00665-3","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Background&lt;/h3&gt;&lt;p&gt;The rise in global temperatures and subsequent increase in extreme heatwaves is implicated to have a significant contribution to the growing incidence of various skin diseases, particularly melanoma skin cancer. The existing therapeutic approaches for skin cancer such as chemotherapy induce significant adverse effects. This highlights the need for further development of novel medicinal substances from natural sources which has less side effects implications. Macroalgae, often known as seaweeds, are recognized for their diverse array of bioactive compounds, which have numerous potential applications. The red coralline macroalgae or seaweed &lt;i&gt;Mastophora&lt;/i&gt; &lt;i&gt;rosea&lt;/i&gt; is abundant in the tropical Indo-Pacific region, including Indonesia. However, little is known regarding the phytochemical profile and potent bioactivity of this unique seaweed. The objective of this study is to assess the phytochemical profile, antioxidant and cytotoxic activity of &lt;i&gt;M.&lt;/i&gt; &lt;i&gt;rosea&lt;/i&gt; in melanoma cell line.&lt;/p&gt;&lt;h3&gt;Methodology&lt;/h3&gt;&lt;p&gt;The dried macroalgae &lt;i&gt;M.&lt;/i&gt; &lt;i&gt;rosea&lt;/i&gt; sample was extracted using various solvents with differing polarities: ethanol, ethyl acetate (EA), and n-hexane. Subsequent biochemical evaluations included total phenolic content and antioxidant capability. Additionally, cytotoxic experiments were conducted using the mammalian melanoma cell line SK-MEL-2.&lt;/p&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;p&gt;The ANOVA results revealed that the EA solvent was the most efficient in producing extracts with high total phenolic content (368.81 ± 3.16 mg GAE/g). This high total phenolic content is accompanied by potent antioxidant capacity (IC&lt;sub&gt;50&lt;/sub&gt; = 99.52 ± 19.58 µg/mL). In addition, &lt;i&gt;M.&lt;/i&gt; &lt;i&gt;rosea&lt;/i&gt; EA extract is attributed with the presence of various polyphenolic catechins, such as gallic acid, gallocatechin, epigallocatechin (EGC), catechin, epicatechin, epigallocatechingallate (EGCG), and epicatechingallate (ECG). Furthermore, &lt;i&gt;M.&lt;/i&gt; &lt;i&gt;rosea&lt;/i&gt; EA shows strong cytotoxic activity against the melanoma cell line SK-MEL-2 (IC&lt;sub&gt;50&lt;/sub&gt; = 37.10 ± 0.85 µg/mL). In addition to the melanoma cell line, the &lt;i&gt;M.&lt;/i&gt; &lt;i&gt;rosea&lt;/i&gt; EA extract shows similar cytotoxicity in epithelial HeLa cells (IC&lt;sub&gt;50&lt;/sub&gt; = 34.32 ± 1.19 µg/mL) and low cytotoxicity in normal human dermal fibroblast cells (IC&lt;sub&gt;50&lt;/sub&gt; &gt; 200 µg/mL).&lt;/p&gt;&lt;h3&gt;Conclusion&lt;/h3&gt;&lt;p&gt;The current results show that &lt;i&gt;M.&lt;/i&gt; &lt;i&gt;rosea&lt;/i&gt; could induce apoptosis and also suppress metastasis activity in SK-MEL-2 cells. Further characterization and isolation of the possible bioactive compounds are necessary to better comprehend its mechanism of action against melanoma. Nevertheless, this study indicates that more study needed to be done on various macroalgae regarding their phenolic components that may be beneficial for the development of novel therapeutic agents for cancer treatment.&lt;/p&gt;&lt;h3&gt;Graphical abstract&lt;/h3&gt;&lt;div&gt;&lt;figure&gt;&lt;div&gt;&lt;div&gt;&lt;picture&gt;&lt;source&gt;&lt;img&gt;&lt;/source&gt;&lt;/pict","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-025-00665-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145161261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bag Valve Mask with Ventilation Indicator (BVM-VI) prototype for ventilation rhythm in Basic Life Support","authors":"Pratama Yulianto,&nbsp;Endang Triyanto,&nbsp;Ridlwan Kamaluddin,&nbsp;Adhe Akbar Azanni","doi":"10.1186/s43088-025-00664-4","DOIUrl":"10.1186/s43088-025-00664-4","url":null,"abstract":"<div><h3>Background</h3><p>Bag valve mask (BVM) are a practical choice for emergency medical personnel to treat out-of-hospital cardiac arrest (OHCA). However, the use of currently available conventional BVM still often results in overventilation in cases of cardiac arrest or respiratory failure. Globally, 79% of hyperventilation events occur during Basic Life Support (BLS) with BVM. This study aims to produce a prototype BVM-VI.</p><h3>Method</h3><p>The prototyping method of Research and Development design was chosen as the primary method, followed by black box testing with clinical, practical, and academic experts, and ended with limited scale testing with 10 users.</p><h3>Result</h3><p>BVM-VI is the result of combining conventional BVM with a portable indicator light module to signal ventilation actions. Expert assessment using the black box test method with CVI analysis resulted in a content validity score of 100%. A limited study conducted by nursing students using a survey method showed reliability with an Intraclass Correlation Coefficient (ICC) of 0.769.</p><h3>Conclusion</h3><p>BVM-VI became the initial prototype for developing a ventilation aid with breathing rhythm adjustment in BLS.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-025-00664-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145160810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research progress on the application of near-infrared spectroscopy in liquid food quality testing","authors":"Wenliang Qi,&nbsp;Qingqing Jiang,&nbsp; Erihemu","doi":"10.1186/s43088-025-00660-8","DOIUrl":"10.1186/s43088-025-00660-8","url":null,"abstract":"<div><p>Near-infrared spectroscopy is a spectral analysis technique that involves electromagnetic radiation at wavelengths between the visible spectrum and the mid-infrared spectrum. The technique can realize non-destructive testing of samples and is particularly suitable for rapid analysis in the field and online quality monitoring. This technique utilizes the absorption of near-infrared light by hydrogen-containing groups within a sample, enabling the extraction of specific information regarding the characteristics of these hydrogen-containing groups within the analyzed organic molecules. The application of chemometrics in near-infrared spectral analysis reduces the data set size, excludes noise interference and improves the efficiency of multivariate correction. The finite variable model construction reduces the random errors and improves the accuracy of the prediction results. The present document synthesizes the application of near-infrared spectroscopy and chemometrics in the context of liquid food safety detection. It provides a comprehensive overview of the state-of-the-art research in this field, emphasizing the role of chemometrics analysis in evaluating the quality and safety of liquid foods. Additionally, it delves into the merits and limitations of this technological approach, offering insight into its potential for future advancement and expansion. </p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-025-00660-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145171037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation of Aloe vera extract-loaded chitosan nanoparticles for the controlled delivery of extract phytochemicals in carbon tetrachloride-induced liver injury rat model","authors":"Omar Ashraf,&nbsp;Alyaa Farid,&nbsp;Gehan Safwat","doi":"10.1186/s43088-025-00663-5","DOIUrl":"10.1186/s43088-025-00663-5","url":null,"abstract":"<div><h3>Background</h3><p>A significant contributing factor to liver damage is drug consumption. Phytochemicals of <i>Aloe vera</i> extract are effective against a variety of diseases. Consequently, this study aimed to create chitosan nanoparticles (chi NPs) that were loaded with <i>Aloe vera</i> extract (ALV extract) to increase the delivery of the extract's bioactive materials. ALV extract, chi NPs, and <i>Aloe vera</i> extract-loaded chitosan nanoparticles (ALV-chi NPs) underwent anti-oxidant, anti-inflammatory, and cytotoxicity tests. The preventive and therapeutic effects of ALV-chi NPs against carbon tetrachloride (CCl4)-induced liver injury were assessed using a male Sprague Dawley rat model.</p><h3>Results</h3><p>Our findings demonstrated that the synthesis of ALV-chi NPs was a promising option for combining the therapeutic benefits of both ALV extract (included in its phytochemicals) and chi NPs. ALV-chi NPs have a uniformly distributed smooth shape with a size of 48.3 ± 2.97 nm, similar to the hydrodynamic size (50.9 ± 0.07 nm), and a surface charge of 38.16 mV. At a 1000 μg/mL concentration, ALV-chi NPs showed high DPPH scavenging % and a high hemolysis inhibition %. At 75 μg/mL, ALV-chi NPs showed lower PTT (63.7 s) than ALV extract (71.2 s). The phenolic components and flavonoids in the extract were released under controlled conditions throughout time, and their bioavailability was enhanced by loading the extract on chi NPs.</p><h3>Conclusions</h3><p>Among all tested formulations, ALV-chi NPs demonstrated superior efficacy, showing 95.4% DPPH scavenging (vs. 91.8% for free extract), 94.1% hemolysis inhibition (comparable to indomethacin), and optimal hepatoprotection in CCl4-induced liver injury. ALV-chi NPs ameliorated the raised levels of liver function parameters, pro-inflammatory cytokines, and intracellular apoptotic proteins.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-025-00663-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145171730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial effects of nano-emulsified cumin oil on performance and carcass characteristics in weaning rabbits infected by Clostridium perfringens type A","authors":"Reda. R. Fathy,&nbsp;Mohamed Abaza,&nbsp;Zangabel Mohamed,&nbsp;Aya. H. Tantawy,&nbsp;Mona Abdallah,&nbsp;Noura M. khalaf,&nbsp;Sherein Mohamed","doi":"10.1186/s43088-025-00659-1","DOIUrl":"10.1186/s43088-025-00659-1","url":null,"abstract":"<div><h3>Background</h3><p>This study aimed to evaluate the antibacterial effects and growth-promoting potential of nano-emulsified cumin oil (NECO) in vitro and in vivo trials using newly weaned Gabaly rabbits. NECO was tested as both a preventive and curative agent against <i>Clostridium perfringens</i> type A infection through dietary supplementation.</p><h3>Methods</h3><p>The study included two experimental phases: (1) an in vitro trial to determine the bacterial inhibition potential of different NECO concentrations (0.1, 0.2, 0.5, and 1 mL) and (2) an in vivo trial using 120 four-week-old newly weaned male Gabaly rabbits (0.544 kg average body weight) allocated into five groups (<i>n</i> = 24/group were divided into 3 replicates, 8 animals each). The groups were: G1 (negative control, no infection or supplement), G2 (NECO control, supplemented with 10 mL/kg diet), G3 (positive control, infected with <i>C. perfringens</i> type A, no supplement), G4 (preventive NECO, supplemented with 10 mL/kg diet before infection), and G5 (curative NECO, supplemented with 10 mL/kg diet after infection). NECO was formulated as a stable nano-emulsion using a high-shear homogenizer.</p><h3>Results</h3><p>The in vitro study demonstrated that NECO inhibited <i>C. perfringens</i> growth at all tested concentrations, with complete inhibition observed at 1 mL. Based on this, the 1 mL concentration was selected for in vivo trials. Infected rabbits (G3) exhibited clinical signs including anorexia, depression, severe diarrhea, and bloat, with a morbidity rate of 90% and a 35% mortality rate by the 5th day post-challenge. NECO supplementation significantly improved (<i>p</i> &lt; 0.05) final body weight (FBW), total weight gain, and average daily gain (ADG) in G2 compared to G3. While G4 and G5 did not significantly differ in FBW or ADG from the control (G1), all NECO-supplemented groups had improved feed conversion ratio (FCR) compared to G3 (<i>p</i> &lt; 0.05). The best FCR was observed in G2 (3.80), followed by G4 (4.51) and G5 (4.77), while the worst was in G3 (5.31). Histopathological and postmortem findings confirmed reduced disease severity with NECO supplementation.</p><h3>Conclusion</h3><p>These results suggest that NECO serves as a natural antibacterial alternative, effectively mitigating <i>C. perfringens</i> infection while enhancing growth performance.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-025-00659-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145171075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular and morphological characterization of Chrysomya bezziana (Diptera: Calliphoridae) in Egypt","authors":"Salwa S. Rashed,&nbsp;Emad I. M. Khater,&nbsp;Rawda M. Badawy,&nbsp;Abdalla H. Radwan,&nbsp;Eman E. Zaher","doi":"10.1186/s43088-025-00657-3","DOIUrl":"10.1186/s43088-025-00657-3","url":null,"abstract":"<div><h3>Background</h3><p><i>Chrysomya bezziana</i> (Villeneuve, 1914), the screw-worm fly, is a significant parasitic blow fly species that causes obligatory myiasis in livestock and, in some cases, in humans. Its ability to infest wounds and cause extensive tissue damage makes it a significant concern in the veterinary and public health sectors. Accurate and timely identification of <i>C. bezziana</i> is critical for managing outbreaks and implementing control measures. This study confirms the occurrence of <i>C. bezziana</i> in Egypt through both morphological and molecular identification, as only a few studies have reported its presence in Egypt so far. Adult samples of <i>C. bezziana</i> were collected using meat-baited traps situated in the cemeteries of two localities in Egypt (Port Said and El-Sharkia Governorates). DNA was extracted from the specimens for molecular identification using the cytochrome oxidase I (<i>cox1</i>) gene.</p><h3>Results</h3><p>Morphologically, <i>C. bezziana</i> adult flies were identified by a row of hairs on the dorsal surface of the wing's stem vein, blackish anterior spiracles, and the most distinctive characteristic, a white calypter with a whitish-yellow distal end, which differentiates this species from the most closely related species, <i>Chrysomya megacephala</i>. Genetically, it was found that based on a 703 bp fragment of the <i>cox1</i> gene, <i>C. bezziana</i> was accurately identified, and further phylogenetic analysis confirmed the DNA-based identification of adult specimens of <i>C. bezziana</i> examined. This finding confirms the presence of <i>C. bezziana</i> and expands our knowledge of its distribution in Egypt.</p><h3>Conclusion</h3><p>This study is the first to report the occurrence of <i>C. bezziana</i> in Egypt using an integration of molecular and morphological methods.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-025-00657-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145167626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The potential of microneedle platforms in addressing pediatric drug delivery challenges: perspectives of healthcare professionals and parents","authors":"Heba Y. Raslan,&nbsp;Waleed Faisal,&nbsp;Noura H. Abdellah,&nbsp;Sara A. Abouelmagd,&nbsp;Elsayed A. Ibrahim","doi":"10.1186/s43088-025-00662-6","DOIUrl":"10.1186/s43088-025-00662-6","url":null,"abstract":"<div><h3>Background</h3><p>Pediatric drug delivery faces significant challenges owing to children’s unique physiology and limitations of conventional dosage forms. There is a growing need for age-appropriate, safe, and effective alternatives.</p><h3>Objectives</h3><p>This study aimed to assess the challenges in selecting pediatric pharmaceutical dosage forms and to evaluate the potential of microneedles (MNs) as an innovative solution, capturing perspectives of healthcare professionals (HCPs) and parents.</p><h3>Methods</h3><p>Two cross-sectional surveys were conducted: (1) a survey for pediatricians (<i>n</i> = 154) on dosage form challenges, and (2) a survey of HCPs and parents (<i>n</i> = 386) on perceptions of MNs for pediatric drug delivery. Responses were analyzed quantitatively and qualitatively.</p><h3>Results</h3><p>Pediatricians identified key barriers: unsuitable formulations (53%), injection distress (38%), and inadequate strength availability (29%). Cost (59%), therapeutic efficacy (60%), and administration route (53%) dominated prescribing decisions. Anti-infectives (69%) and analgesics (47%) were top candidates for reformulation. Respondents (85%) acknowledged needle phobia as a major issue, and 84% expressed willingness to use MNs, peaking at 93% for children aged 1–3 years. MNs’ perceived advantages included reduced needle phobia, improved compliance in chronic diseases, and self-administration potential. Primary concerns included practicality in emergencies (59%), drug-loading capacity (46%), and dosing accuracy.</p><h3>Conclusions</h3><p>Significant unmet needs persist in pediatric drug delivery. MNs demonstrate strong potential to address core challenges, particularly needle aversion and formulation unsuitability, with high acceptance among stakeholders. Translation requires overcoming barriers in manufacturing scalability, regulatory clarity, and user-centered design. Coordinated efforts in education, targeted formulation development, and policy advocacy are essential for clinical integration.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-025-00662-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145167627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Docking investigation of Thevetia peruviana plant compounds for targeting molluscicidal activity against Biomphalaria alexandrina snails, the intermediate host of Schistosoma mansoni","authors":"Samah I. Ghoname,&nbsp;Basma H. Amin,&nbsp;Asmaa T. Mohamed,&nbsp;Olfat A. Hammam,&nbsp;Hebat-Allah A. Dokmak","doi":"10.1186/s43088-025-00624-y","DOIUrl":"10.1186/s43088-025-00624-y","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Background&lt;/h3&gt;&lt;p&gt;&lt;i&gt;Thevetia peruviana&lt;/i&gt;, commonly known as Yellow Oleander or Cabbage Tree, is a tropical shrub. Extracts from various parts of the plant (e.g., leaves, seeds) have demonstrated pesticidal properties, including the ability to repel or kill intermediate hosts of &lt;i&gt;Schistosomiasis.&lt;/i&gt;&lt;/p&gt;&lt;h3&gt;Methods&lt;/h3&gt;&lt;p&gt;This study employed gas chromatography–mass spectrometry (GC–MS) analysis to identify 33 components in the ethanol extract, with key compounds exhibiting antioxidant, anti-inflammatory, anticancer, and antimicrobial properties. Additionally, the research investigated the molluscicidal activity of the plant extract, its impact on Caspase-3 expression, steroid sex hormone levels in &lt;i&gt;Biomphalaria alexandrina&lt;/i&gt;, and histological alterations in the digestive and hermaphrodite glands. Levels of nitric oxide and comet assay results were evaluated in &lt;i&gt;Schistosoma mansoni&lt;/i&gt;-Infected &lt;i&gt;B. alexandrina&lt;/i&gt; snails, comparing them to a control group at the first cercarial shedding stage. The analysis of docking scores provides a quantitative measure of binding affinity, where more negative values indicate stronger interactions. The variation in docking scores highlights the diverse binding potentials of these compounds and emphasizes the importance of considering specific molecular interactions in the context of Caspase-3 modulation.&lt;/p&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;p&gt;The study identified 33 components in the ethanol extract of &lt;i&gt;T. peruviana&lt;/i&gt;, analyzed by GC–MS. Molluscicidal tests demonstrated concentration-dependent sensitivity, with an LC&lt;sub&gt;90&lt;/sub&gt; value of 43.79 ppm. Caspase-3 expression was upregulated in &lt;i&gt;B. alexandrina&lt;/i&gt; snails following treatment with LC&lt;sub&gt;10&lt;/sub&gt; ppm and LC&lt;sub&gt;25&lt;/sub&gt; ppm, affecting steroid sex hormone levels. Histological analysis revealed significant damage to the digestive and hermaphrodite glands, indicating reproductive disturbances post-treatment. At the first cercarial shedding stage, nitric oxide levels increased at LC&lt;sub&gt;10&lt;/sub&gt; ppm and LC&lt;sub&gt;25&lt;/sub&gt; ppm compared to the control group (&lt;i&gt;P&lt;/i&gt; &lt; 0.001). DNA damage percentage also increased with higher treatment concentrations, showing varied responses in &lt;i&gt;S. mansoni&lt;/i&gt;-infected &lt;i&gt;B. alexandrina&lt;/i&gt; snails. The in silico study identified Glycan Sialylated Tetraose Type-2 as the most active compound, exhibiting the highest affinity and the most significant effect against Caspase-3.&lt;/p&gt;&lt;h3&gt;Conclusions&lt;/h3&gt;&lt;p&gt;&lt;i&gt;Thevetia peruviana&lt;/i&gt; extract exhibited concentration-dependent molluscicidal effects on &lt;i&gt;B. alexandrina&lt;/i&gt; snails, impacting key biological processes, such as Caspase-3 expression and sex hormone levels. Histological analysis revealed glandular damage, while increased nitric oxide levels and DNA damage highlighted the extract’s effects on infected snails. Furthermore, the findings emphasize the importance of understanding ligand–protein interactions, providing valuable insights into targeting apoptosis through Ca","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-025-00624-y","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145161699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green silver nanoparticles ameliorate diet-induced obesity through antioxidant and anti-inflammatory properties","authors":"Neveen Madbouly,&nbsp;Asmaa Mahmoud,&nbsp;Aya Mohamed,&nbsp;Joseph Magdy,&nbsp;Manar Hassan,&nbsp;Marina Osama,&nbsp;Mina Adel,&nbsp;Yousab Romany,&nbsp;Alyaa Farid","doi":"10.1186/s43088-025-00656-4","DOIUrl":"10.1186/s43088-025-00656-4","url":null,"abstract":"<div><p>This study aims to evaluate the anti-obesity effect of silver nanoparticles biosynthesized in <i>Aloe vera</i> leaf extract (AV-AgNPs) administrated to Sprague Dawley rats  that fed with high-fat diet (HFD) for up to 12 weeks. AV-AgNPs were recognized by transmission electron microscopy (TEM) and dynamic light scattering. The in vitro study investigated the antioxidant ability, effects on coagulation time, anti-inflammatory, cytotoxicity of AVLE and AV-AgNPs and cytokinesis-block micronucleus (CBMN) assay for genotoxic effects. When the in vivo experiment is completed, different parameters such as body weight, fasting plasma glucose (FPG), serum lipid levels, white adipose tissue (WAD) oxidative stress markers and adipokines were evaluated. Green AV-AgNPs were spherical with sizes of 19.4–25.9 nm as revealed using TEM. The sizes and zeta potentials of AV-AgNPs presented particle stability up to 70 days. AV-AgNPs had a high in vitro antioxidant and anti-inflammatory abilities with no significant effects on partial thromboplastin time and the prothrombin time. In vitro CBMN assay indicated no significant genotoxic effects of AVLE or AV-AgNPs at low concentration (25 µg/ml). AV-AgNPs and AVLE showed significant decline in body FPG and lipid profile in HFD-fed rats with no apparent effects on normal pellet diet (NPD)-fed ones. In addition, oxidative stress markers, size of subcutaneous adipocytes and micro-vesicular steatosis of hepatocytes in the HFD group were significantly reduced after AVLE and AV-AgNPs administration. Finally, WAD analysis of M2 interleukin (IL)-4 and IL-10 was significantly elevated with AVLE and AV-AgNPs supplementation. Further, both significantly lowered M1 pro-inflammatory cytokines as tumor necrosis factor-α (TNF-α), IL-1β and IL-6 in the WAD of HFD-fed group. In terms of body weight, adiposity and hepatic steatosis, the anti-obesity properties of AV-AgNPs were significant (34.4–35.17% weight reduction) compared to whole AVLE (13.9–18.36% weight reduction). The biochemical and immunological effects were comparable and mediated by amelioration of oxidative stress and induction M2 polarization in WAD.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-025-00656-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145161265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}