Beni-Suef University Journal of Basic and Applied Sciences最新文献

{"title":"Exploring and detecting predictors associated with survival and mortality of cervical cancer patients: a 10-year retrospective study","authors":"Shaba Muneeha,&nbsp;Raushan Kumar Chaudhary,&nbsp;Vijith Vittal Shetty,&nbsp;Soumya Patil,&nbsp;Uday Venkat Mateti","doi":"10.1186/s43088-025-00602-4","DOIUrl":"10.1186/s43088-025-00602-4","url":null,"abstract":"<div><h3>Background</h3><p>Cervical cancer is the 4th most prevalent cancer among females globally. In India, approximately 123,907 women are diagnosed with cervical cancer every year, leading to 77,348 deaths annually. However, Indian healthcare system lacks the sufficient information regarding the factors influencing survival and mortality among cervical cancer patients at regional levels. Thus, we aimed to identify the predictors associated with survival outcomes and mortality rates among cervical cancer.</p><h3>Methods</h3><p>A retrospective cohort study was conducted over 8 months at a tertiary care hospital where 10-year (January 2013–December 2022) data of cervical cancer patients were analyzed from medical record department (MRD). Telephonic interviews were carried out with patients or patient parties to know the survival status of patients. The data was analyzed using descriptive statistics, Kaplan–Meier curve, log-rank test and Cox regression.</p><h3>Results</h3><p>Out of 330 cervical cancer patients, majority (64.24%) were &gt; 50 years of age followed by 35.76% were &lt; 50 years. Most of the patients had abnormal body mass index (BMI) (46.96%), postmenopausal stage (75.76%), stage II cancer (43.03%), histologically poorly differentiated grade (47.88%) and squamous cell carcinoma (87.88%), with radiation plus chemotherapy being popular treatment choice (48.79%) and with the overall mean age of 56 years. Age, BMI, menopause, stage of cancer, histological grades and types of treatment were found to be significant predictors (<i>p</i> &lt; 0.05) of survival among cervical cancer patients. Using cox regression analysis, advanced age (age &gt; 50 years: hazard ratio (HR): 1.82), underweight (BMI &lt; 18.5: HR:1), postmenopause (HR:1), advanced stage of cervical cancer (Stage I, Stage II, Stage III, Stage IV: HR:1, HR:2.78, HR:10.08, HR:20.81), poorly differentiated cervical cancer (HR:1.70), radiation therapy (HR:4.86), chemotherapy (HR:6.55) or chemoradiation therapy (HR:3.31) and surgery plus chemotherapy (HR: 4.55) were identified to be significant predictors of mortality among cervical cancer patients.</p><h3>Conclusion</h3><p>We conclude that the 5- and 10-year survival rates for cervical cancer patients were found to be 51.2% and 42.9%, respectively. Advanced age, underweight, postmenopausal status, advanced cancer stage, poor cancer cell differentiation and chemotherapy-based treatment were significant predictor of mortality and vice-versa for survival which might guide clinicians and policymakers in making informed clinical decisions to combat cervical cancer.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-025-00602-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143108260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discovering the anti-parasitic activity of melatonin loaded lecithin/chitosan nanoparticles against giardiasis and cryptosporidiosis in Balb/c infected mice","authors":"Mohamed Nasser,&nbsp;Mona B. Abd El-atif,&nbsp;Habiba Alaa,&nbsp;Mariam Abdelaziz,&nbsp;Mariam Mustafa,&nbsp;Mustafa Masour,&nbsp;Salma Magdy,&nbsp;Salma Mohsen,&nbsp;Yomna El Karamany,&nbsp;Alyaa Farid","doi":"10.1186/s43088-024-00588-5","DOIUrl":"10.1186/s43088-024-00588-5","url":null,"abstract":"<div><h3>Background</h3><p><i>Giardia duodenalis</i> and <i>Cryptosporidium parvum</i> are the primary causes of diarrhea with global attention due to the severe pathophysiological changes leading to mortality. During this study, we explored the biological protozoal contaminants (<i>Giardia</i> and <i>Cryptosporidium</i> spp.) in some areas of the Nile River. Then, we evaluated effectiveness of melatonin (Mel) and melatonin loaded lecithin/chitosan nanoparticles (Mel-LCNPs) against giardiasis and cryptosporidiosis in mice models using parasitological and inflammatory response examination.</p><h3>Results</h3><p>The number of positive samples for <i>Cryptosporidium</i> was higher than that for <i>Giardia</i> with percentage of 46.67% and 40.0%, respectively. Prior to treatment, the physical characterization (hydrodynamic size and zeta potential) and in vitro characterization of Mel-LCNPs were carried. Mel-LCNPs revealed a hydrodynamic size of 78.8 nm and a zeta potential of − 27.2 mV. Furthermore, they have powerful antioxidant and anti-inflammatory properties, while displaying minimal anticoagulant and cytotoxic effects during in vivo evaluation. Mel was consistently discharged from nanoparticles in a regulated and enduring manner for 36 h. Moreover, Mel in NPs has an entrapment efficiency (EE) of 33.6% and a drug loading capacity (DLC) of 7.2% and significant reduction (100% and 99.4%, respectively) in the shedding of <i>Giardia cysts</i> and <i>Cryptosporidium oocysts</i>. This reduction was higher than that observed with Mel alone or LCNPs alone on the 14th day post-infection. Moreover, mice of group V, which received Mel-LCNP treatment, exhibited significantly normal levels of interleukin-4 (IL-4) and interferon-gamma (IFN-γ) as well as healthy control mice group (group I).</p><h3>Conclusion</h3><p>Mel-LCNPs were highly effective preparations against giardiasis and cryptosporidiosis in Balb/c mice experimentally infected with proved antioxidant, anti-inflammatory, and immunological modulatory characteristics.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-024-00588-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143107874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and genetic analysis of the recent Avian pox viruses from infected pigeons and chickens","authors":"Amira M. Helal,&nbsp;Osama Mahana,&nbsp;Ali Zanaty,&nbsp;Karim Selim,&nbsp;Mohamed. E. Taha,&nbsp;Mostafa M. Saleh,&nbsp;Mohamed I. Hassan,&nbsp;Mona S. Abdallah","doi":"10.1186/s43088-024-00589-4","DOIUrl":"10.1186/s43088-024-00589-4","url":null,"abstract":"<div><h3>Background</h3><p>Avian pox viruses (APVs) are highly contagious poultry diseases, posing a major economic threat to poultry production systems, and have a high mortality rate among young birds, Infected birds also face condemnation of affected carcasses due to the unsightly appearance of the nodular skin lesions.</p><p>This study aimed to provide an overview of the current genetic status of APV in backyard poultry, with a focus on the commercially available vaccines. To achieve this, molecular characterization and phylogenetic analysis of APVs were conducted, comparing their sequences with vaccine strains used in Egypt.</p><h3>Results</h3><p>The polymerase chain reaction (PCR) assay was able to detect APV in all the tested samples; 12 positive samples (6 chicken flocks and 6 pigeon flocks) were selected for DNA sequencing. The sequences were submitted to GenBank with accession numbers OR027032 to OR027043. The chicken strains exhibited 100% nucleotide identity with commercially available fowl pox virus (FPV) vaccines and were phylogenetically clustered within subclade A1 with other FPVs. On the other hand, the pigeon pox virus (PPV) strains were closely related to other PPV strains within subclade A2 and showing 100% and 91% nucleotide identity with the PPV and the FPV vaccines, respectively.</p><h3>Conclusions</h3><p>Despite the availability of APV vaccines in Egypt, a persistent threat of APV to poultry in the backyard system remains a significant concern. Our molecular characterization revealed the high genetic similarity between our field strains and commercially available vaccine strains, suggesting an urgent need for vaccination in backyard systems to counteract this emerging threat to bird populations.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-024-00589-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143110169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction Note: Histological, ultrastructural, and biochemical study on the possible role of Panax ginseng in ameliorating liver injury induced by Lambda cyhalotherin","authors":"Manal Abdul-Hamid,&nbsp;Hanaa M. Mohamed,&nbsp;Sanaa M. Abd El-Twab,&nbsp;Karim Zaied","doi":"10.1186/s43088-025-00600-6","DOIUrl":"10.1186/s43088-025-00600-6","url":null,"abstract":"","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-025-00600-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143110102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fish-derived hepcidins in cancer treatment: a scoping review","authors":"Wan-Atirah Azemin,&nbsp;Syarifah Ab Rashid,&nbsp;Noraini Philip,&nbsp;Abdul Manaf Ali,&nbsp;Mohd Shahir Shamsir","doi":"10.1186/s43088-025-00599-w","DOIUrl":"10.1186/s43088-025-00599-w","url":null,"abstract":"<div><h3>Background</h3><p>The discovery of hepcidin, a peptide hormone primarily known for iron homeostasis regulation, has revealed promising anticancer properties. While extensively studied in mammals, fish-derived hepcidins represent an unexplored area in cancer therapeutics, offering unique structural and functional characteristics that may prove valuable in oncological applications.</p><h3>Method</h3><p>A scoping review was conducted using the Scopus, Web of Science (WoS), and PubMed databases to comprehensively analyse published literature on fish-derived hepcidins. Publications were identified using Boolean combinations of ‘fish’, ‘hepcidin’, and ‘cancer’. Two independent reviewers screened articles, with a third reviewer resolving disagreements. Research themes were categorised and analysed with focus on species distribution, mechanisms of action, and therapeutic potential.</p><h3>Result</h3><p>Analysis of 881 publications revealed research distribution across four main categories: immune response (60.07%), antimicrobial peptides (17.65%), iron homeostasis (13.69%), and cancer research (2.94%). The review identified 17 fish species with documented hepcidin studies. Tilapia-derived hepcidins demonstrated notable anticancer properties, including concentration-dependent effects, selective cytotoxicity against cancer cells, and potential enhancement of conventional chemotherapy efficacy through mechanisms involving reactive oxygen species production and mitochondrial apoptosis pathways.</p><h3>Conclusion</h3><p>Despite promising anticancer properties of fish-derived hepcidins, particularly from tilapia, significant knowledge gaps exist in understanding their cancer-specific mechanisms and clinical applications. Future research should prioritise broader species investigation, safety profiling, and delivery system development to advance their therapeutic potential in cancer treatment.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-025-00599-w","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143109572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of thymol and propolis extract on genotoxicity, biochemical activities and expression profile of some genes on honey bee, Apis mellifera, infected with Vairimorpha (Nosema) ceranae","authors":"Salwa I. Sebak,&nbsp;Heba A. S. Elelimy,&nbsp;Heba Seyam,&nbsp;Soad A. Elkenawy","doi":"10.1186/s43088-025-00596-z","DOIUrl":"10.1186/s43088-025-00596-z","url":null,"abstract":"<div><h3>Background</h3><p><i>Nosema ceranae,</i> the predominant microsporidian parasite, weakens <i>Apis mellifera</i> honey bee colonies and reduces their productivity and reproduction. This research aims to detect the impact of thymol and propolis extracts on genotoxicity repair, oxidative enzymes, expression of some antimicrobial genes and some hypopharyngeal glands genes in <i>N. ceranae</i>-infected bees. Experimentally, infected honeybee colonies were divided into four groups: (1) untreated, (2) for propolis (3 g/L) treatment, (3) for thymol (0.1 g/L) treatment and (4) for mixed treatment with thymol and propolis. Workers from adult honeybee colonies, treated and untreated, were randomly sampled. The collected samples were used in studying the genotoxicity effect, the concentration of oxidative enzymes (superoxide-dismutases, glutathione S transferase, catalase and malondialdehyde (MDA)), measuring the relative expression of antimicrobial genes and hypopharyngeal glands genes major royal proteins 1 &amp; 2 (MJRP1 and MJRP2).</p><h3>Results</h3><p>The results show that thymol and propolis extract decreased genotoxicity effect, increased expression of antimicrobial genes, increased expression levels of MJRP1 and MJRP2 genes and decreased activities of oxidative enzymes when applied to <i>Nosema</i>-infected bees.</p><h3>Conclusion</h3><p>Thymol and propolis extract positively affect honey bee health and mix of them. This study reveals that natural product extracts and their mixture may fight <i>Nosema</i> and prevent honey bee colonies decline and sudden death.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-025-00596-z","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143109610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recognition model for major depressive disorder in Arabic user-generated content","authors":"Esraa M. Rabie,&nbsp;Atef F. Hashem,&nbsp;Fahad Kamal Alsheref","doi":"10.1186/s43088-024-00592-9","DOIUrl":"10.1186/s43088-024-00592-9","url":null,"abstract":"<div><h3>Background</h3><p>One of the psychological problems that have become very prevalent in the modern world is depression, where mental health disorders have become very common. Depression, as reported by the WHO, is the second-largest factor in the worldwide burden of illnesses. As these issues grow, social media has become a tremendous platform for people to express themselves. A user’s social media behavior may therefore disclose a lot about their emotional state and mental health. This research offers a novel framework for depression detection from Arabic textual data utilizing deep learning (DL), natural language processing (NLP), machine learning (ML), and BERT transformers techniques in light of the disease’s high prevalence. To do this, a dataset of tweets was used, which was collected from 3 sources, as we mention later. The dataset was constructed in two variants, one with binary classification and the other with multi-classification.</p><h3>Results</h3><p>In binary classifications, we used ML techniques such as “support vector machine (SVM), random forest (RF), logistic regression (LR), and Gaussian naive Bayes (GNB),” and used BERT transformers “ARABERT.” In comparison ML with BERT transformers, ARABERT has high accuracy in binary classification with a 93.03 percent accuracy rate. In multi-classification, we used DL techniques such as “long short-term memory (LSTM),” and used BERT transformers “Multilingual BERT.” In comparison DL with BERT transformers, multilingual has high accuracy in multi-classification with an accuracy of 97.8%.</p><h3>Conclusion</h3><p>Through user-generated content, we can detect depressed people using artificial intelligence technology in a fast manner and with high accuracy instead of medical technology.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-024-00592-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143109384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combination therapy of intravitreal ranibizumab and sub-tenon triamcinolone for treatment of resistant diabetic macular edema: a clinical study in Egypt","authors":"Hossam Eldin M. A. Khalil,&nbsp;Hazem E. Haroun,&nbsp;Alaa A. I. Shalan,&nbsp;Waleed M. Mahran","doi":"10.1186/s43088-025-00598-x","DOIUrl":"10.1186/s43088-025-00598-x","url":null,"abstract":"<div><h3>Background</h3><p>Among those aged 20–74 in industrialized countries, diabetic retinopathy (DR) is the main cause of visual impairment. Diabetic macular edema (DME) is the leading cause of blindness in people with DR. DME that is resistant to therapy is now being treated with a number of different management strategies. This research was to examine the efficacy of sub-tenon steroid and anti- vascular endothelial growth factor (VEGF) injections as a combination therapy for the treatment of resistant DME, owing to the synergistic effect of this combination.</p><h3>Methods</h3><p>This is a two-arm, randomized, prospective clinical trial that included 100 eyes of patients with refractory DME divided into 2 equal groups: group 1 received posterior subtenon triamcinolone (STTA) and anti-VEGF injections (0.5 mg ranibizumab), and group 2 received anti-VEGF injections (0.5 mg ranibizumab) only, in the same session. The 2 groups were followed up for a period of 6 months.</p><h3>Results</h3><p>Group 1 showed significant improvements in best corrected visual acuity (BCVA) (from 0.20 ± 0.11 to 0.32 ± 0.12, <i>p</i> = 0.04) and central macular thickness (CMT) (from 393.2 ± 35.29 to 260.2 ± 11.43 µm, <i>p</i> = 0.001), with fewer injections required compared to Group 2. Recurrence rates were significantly higher in Group 2 (42% vs. 12%, <i>p</i> = 0.026). After injections, there was a noticeable rise in intraocular pressure (IOP) (16.02 ± 1.56 Vs 16.26 ± 1.24 in both groups respectively). However, this elevation is usually just transitory lasting for short periods of time and is within the safe, insignificant rise ranges.</p><h3>Conclusion</h3><p>The use of combined therapy with anti-VEGF treatment and STTA has been found to be an effective and safe approach to managing resistant DME. The lower number of injections needed help to reduce the economic burden, especially under constrained financial circumstances.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-025-00598-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143109261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Apoptosis induction and tumor growth suppression by hydroxyapatite nanoparticles–cisplatin combined treatment in Ehrlich solid carcinoma-bearing mice","authors":"Shaymaa M. Eissa,&nbsp;Asmaa M. Mahfouz,&nbsp;Saad M. El-Gendy,&nbsp;Al-shimaa Zakaria,&nbsp;Heba Effat,&nbsp;Hanan R. H. Mohamed","doi":"10.1186/s43088-025-00595-0","DOIUrl":"10.1186/s43088-025-00595-0","url":null,"abstract":"<div><h3>Background</h3><p>Hydroxyapatite (HAP) resembles the components of biological hard tissue. Recent research has been interested in the biomedical application of HAP nanoparticles (HAP-NPs) in cancer treatment, HAP-NPs have high cytotoxic activity against cancerous cells, in addition, they are nontoxic to healthy normal cells, biocompatible, biodegradable, and have a high absorption rate within the tissue. Therefore, this study evaluated HAP-NPs' antitumoral activity in Ehrlich solid carcinoma (ESC)-bearing mice, in addition, we examined the anticancer efficacy of combined treatment of a common chemotherapeutic drug such as Cisplatin (CDDP) and HAP-NPs in ESC-bearing mice.</p><h3>Methods</h3><p>Forty female mice were inoculated with 200 µl of diluted ascites fluid containing approximately two million viable cancer cells in the mice's left thigh, after 14 days of inoculation, the mice were distributed into four groups: 10 mice in each. ESC group was administrated distilled water, the HAP-NPs group was treated orally with 100 mg/kg of hydroxyapatite nanoparticles, the CDDP group was administrated intraperitoneally with 5 mg/kg of Cisplatin, the HAP-NPs + CDDP group was treated with both doses of hydroxyapatite and cisplatin, the animal treatment was conducted for 20 days. Antitumor activity was assessed for two durations after 10 and 20 days. DNA damage assessment was performed using comet assay in ESC, in addition, we measured the expression of the following genes (<i>P53, Bcl2,</i> and <i>Bax</i>,) using quantitative real-time PCR, and the apoptotic-related proteins (P53 and Ki-67) using immunohistochemical analysis. A histopathological examination of ESC was performed.</p><h3>Results</h3><p>The obtained data illustrated a promising anticancer activity of HAP-NPs, and the combined treatment of HAP-NPs and CDDP illustrated a higher anticancer efficacy. HAP-NPs, CDDP, and HAP-NPs + CDDP resulted in significant (<i>P</i> &lt; 0.05) nucleic acid destruction, and significant (<i>P</i> &lt; 0.05) overexpression of apoptotic-related genes (<i>P53, Bax</i>, and <i>Bcl2</i>) and proteins (Ki-67 and P53), causing the tumor bulk to be greatly reduced in HAP-NPs, CDDP, and HAP-NPs + CDDP (1100, 570, and 450 mm³), respectively, compared to ESC group was 2240 mm³.</p><h3>Conclusion</h3><p>Hydroxyapatite nanoparticles can provoke DNA damage and regulate apoptosis, selectively eliminating tumor cells. The co-administration of HAP-NPs and CDDP resulted in a synergistic enhancement of cisplatin activity within the tumor tissue.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-025-00595-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cell-free supernatants from plant growth-promoting rhizobacteria Bacillus albus strains control Aspergillus flavus disease in peanut and maize seedlings","authors":"Lai Loi Trinh,&nbsp;Kim Ngoc Le,&nbsp;Hoang Anh Le Lam,&nbsp;Hoai Huong Nguyen","doi":"10.1186/s43088-025-00594-1","DOIUrl":"10.1186/s43088-025-00594-1","url":null,"abstract":"<div><h3>Background</h3><p><i>Aspergillus flavus</i>, a seed-borne fungal pathogen, colonizes host plants and exploits nutrients, hindering the growth of seedlings such as peanut and maize. This study investigates the effectiveness of cell-free supernatants (CFSs) from the plant growth-promoting rhizobacteria (PGPR) <i>Bacillus albus</i> strains NNK24 and NDP61, which belong to the <i>Bacillus cereus</i> group, in suppressing <i>A. flavus</i> AF1.</p><h3>Results</h3><p>The antifungal activity of these CFSs was attributed to their surfactant properties and chemical composition. These were characterized using rapid chemical assays and ultra-high-performance liquid chromatography-electrospray ionization-quadrupole time-of-flight/mass spectrometry (UHPLC-ESI-QTOF/MS), combined with bioinformatic tools such as Global Natural Product Social Molecular Networking (GNPS) and Natural Products Atlas (NPAtlas). Identified putative antifungal compounds included two diketopiperazines (cyclo(Pro-Leu) and cyclo(2-hydroxy-Pro-Leu)), four macrolactins (7-O-succinyl macrolactin A, 7-O-methyl-5′-hydroxy-3′-heptenoate-macrolactin, macrolactin B, and macrolactin C), two siderophores (petrobactin and bacillibactin), and three cyclic lipopeptides (kurstakin 1, 2 or 3, and 4). These compounds are hypothesized to act synergistically via multiple mechanisms, including disruption of fungal membranes, iron capture, direct antibiosis, and triggering plant immunity. Both CFSs strongly suppressed the harmful effects of <i>A. flavus</i> AF1 and seed-borne <i>A. flavus</i> on peanut and maize seedlings, reducing disease incidence (DI) and disease severity index (DSI) compared to controls. The disease control efficacy (DCE) of the CFSs was comparable to that of the commercial fungicide. Additionally, the CFSs enhanced seed germination, vigor, seedling length, and weight in both peanut and maize. Vigor index (VI) values increased by 222.4–286.0% in peanuts and 181.7–216.4% in maize at 7 days after treatment (DAT).</p><h3>Conclusion</h3><p>CFSs of <i>B. albus</i> NNK24 and NDP61 show significant potential as bioprotective agents for sustainable agriculture. Importantly, their use eliminates the need for live bacterial cells from the <i>B. cereus</i> group, addressing biosafety concerns.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-025-00594-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}