Beni-Suef University Journal of Basic and Applied Sciences最新文献

{"title":"The impact of contaminated water with pharmaceutical hazards on the Vicia faba plant cell's components and mitosis behavior","authors":"Mariam Alketbi,&nbsp;Rania Ali,&nbsp;Abdullah A. L. Mutery","doi":"10.1186/s43088-025-00628-8","DOIUrl":"10.1186/s43088-025-00628-8","url":null,"abstract":"<div><h3>Introduction</h3><p>Paracetamol may have its potential risk to ecosystem when pollute water resources around the hospitals and medicines’ factories areas. Water recycling systems are admitted for irrigation purposes to cover the high needs in water demands. The pharmaceutical hazards in the recycled water should be investigated for its effect on plants’ stability and performance.</p><h3>Method</h3><p>This work carried the cytogenetics and cytological studies to visualize the plants performance under paracetamol stress in four concentrations (0, 6, 12.5, 25 and 50%) in two treatment times 3 &amp; 6 h. On the other hand, the recovery experiment for 24 h after treatment was carried to precise the plant cure status. The study is also supported with TEM (transmission electronic microscope) descriptive investigations to many vital cytoplasmic organelles that affect the plant responses, adaptation, energy level and all metabolic process.</p><h3>Results</h3><p>Despite that, the higher used concentration has its cytotoxic and turbo-genic impacts yet, it did not represent any mutagenic threat. It exerted an oxidative stress, chromosomes aberrations (stickiness and disturbances) and genotoxicity (fragmentation) in <i>Vicia faba</i> plant cells. The recovery treatment demonstrated the flexibility of plant detoxification systems to survive and to retain some of its fitness after the influencer disappears.</p><h3>Conclusion</h3><p>Paracetamol affected <i>Vicia faba</i> plant cells in a dose-dependent manner and induced oxidative stress and genotoxicity in <i>Vicia faba</i> plant cells. Therefore, it is important to monitor and regulate the presence of paracetamol in the environment and to develop effective strategies for their removal or degradation from the recycled water as a previous step before being used in plants irrigation.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-025-00628-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143861413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytodiversity and morpho-anatomical modification of the wild plants irrigated by tannery effluents","authors":"Sahar Naveed,&nbsp;Peter Olusakin Oladoye,&nbsp;Syeda Sadaf Zahra,&nbsp;Mansoor Hameed","doi":"10.1186/s43088-025-00629-7","DOIUrl":"10.1186/s43088-025-00629-7","url":null,"abstract":"<div><h3>Background</h3><p>With the rapid increase in industrialization, irrigation through waste or sewage water is a common practice due to low and scarce freshwater supply. Plants are exposed to hazardous metals that accumulate in plant tissues and negatively affect them. Therefore, current study aimed to investigate the phytodiversity and morpho-anatomical modification of wild plants irrigated by tannery effluents. Six wild plant species were collected from different sites of Multan near tannery industries through the quadrate method.</p><h3>Results</h3><p>Results indicated that <i>Cynodon dactylon</i> L. had the highest relative cover, relative frequency, relative density, and important index value, i.e., 35, 10, 30, and 75% followed by other species. The morpho-anatomical modifications showed a reduction in leaf characteristics in <i>C. dactylon</i>, <i>Eragrostis barrelieri</i> Daveau, <i>Polypogon monpeliensis</i> L., <i>Solanum nigrum</i> L., <i>Triticum aestivum</i> L., except in <i>Asparagus adscendens</i> Roxb<i>.</i> A decrease in leaf length was observed in all species with maximum reduction of 55% in <i>T. aestiv</i>um followed by <i>P. monpeliensis</i>. Similarly, reduction in metaxylem area was observed in <i>T. aestivum</i>, whereas sclerenchymatous thickness found to increase in <i>P. monpeliensis</i> by 15%. <i>Asparagus adscendens</i> showed decrease by 70% in comparison to other species.</p><h3>Conclusion</h3><p>Variation in the trend of the morpho-anatomical parameters as well as phytodiversity helps to understand the tolerance potential of the wild plants under wastewater irrigation released by tannery industries and that adverse developmental features in wild plants could be used as bioindicators of toxic heavy metal pollution.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-025-00629-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143861389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Variability in microplastic abundance, bisphenol A contamination, antioxidant properties, and health risks associated with vegetable consumption","authors":"Luqmon Azeez,&nbsp;Rasheed O. Adetoro,&nbsp;Babatunde K. Agbaogun,&nbsp;Abdulrasarq O. Oyedeji,&nbsp;Hassan K. Busari,&nbsp;Abayomi A. Oladejo,&nbsp;Oyeyinka K. Oyelami,&nbsp;Osilaja Deborah,&nbsp;Ruqoyyah D. Oladeji,&nbsp;Saheed Basiru,&nbsp;Shakirat Muhammad-Lawal,&nbsp;Aisha Hammed,&nbsp;Abdulrahman O. Makanjuola","doi":"10.1186/s43088-025-00627-9","DOIUrl":"10.1186/s43088-025-00627-9","url":null,"abstract":"<div><h3>Background</h3><p>Plastic pollution, particularly microplastics (MPs) and toxic additives such as bisphenol A (BPA), endangers human health. Therefore, their routes in the environment need to be investigated. This study investigated microplastic (MPs) abundance, bisphenol A (BPA) levels, and antioxidant activity (AA) in three commonly consumed vegetables—green amaranth, jute mallow, and spinach sourced from two markets in Osogbo, southwestern Nigeria. Microscopic technique was used to determine MP abundance, shapes, and colours. High-performance Liquid Chromatography (HPLC) was used to analyse BPA contents while 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay was used to measure AA. A hazard index (HI) and estimated dietary intakes (EDIs) were used to estimate the associated risks and food safety concerns with MP and BPA in vegetables.</p><h3>Results</h3><p>In this study, MP abundance in shoots ranged from 4.00 ± 0.50 to 7.67 ± 1.04 particles/g and in roots from 5.33 ± 1.53 to 18.00 ± 6.93 particles/g in spinach and green amaranth, respectively, indicating subsoil contamination. Three shapes (fragment, fibre, and irregular) and five colours (transparent, white, yellow, black, and brown) were detected, with fragment shape and transparent colour dominating. Fourier Transform Infrared spectroscopic (FTIR) analysis revealed a predominance of polyethylene, polypropylene, polyethylene terephthalate, polyvinyl chloride, and polyamide microplastics. The BPA ranged from 5.55 ± 0.56 to 6.65 ± 0.00 μg/g while AA ranged from 40.67 ± 6.79 to 72.55 ± 4.03%. A regression analysis and principal component analysis (PCA) were used to identify the relationship among the factors (MPs, BPA, and AA). It is suggested that high levels of MPs and BPA negatively impacted vegetable quality as they are associated with environmental contamination. Contrastingly, AA had a significant positive correlation with vegetable quality. Both EDIs and HI of MPs and BPA were &lt; 1, indicating no significant risk associated with BPA exposure from their consumption.</p><h3>Conclusion</h3><p>This study highlights the potential health risks of MPs and associated BPA contamination in vegetables. The estimated dietary intake and hazard index suggest no immediate health risks, but long-term exposure remains a concern. MPs and BPA in commonly consumed vegetables warrant stricter monitoring of agricultural soil and irrigation water sources. Microplastic exposure in food crops can be reduced with policies that regulate plastic waste disposal and promote sustainable farming practices.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-025-00627-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143848896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic value of focal hyperechoic mesenteric fat sign as an indicator of the primary source of intra-abdominal pathology in patients with abdominal pain","authors":"Rasha Mahmoud,&nbsp;Hanan M. Alfifi,&nbsp;Mohamed Mossad,&nbsp;Naglaa Ezzat Abd Elmageed","doi":"10.1186/s43088-024-00593-8","DOIUrl":"10.1186/s43088-024-00593-8","url":null,"abstract":"<div><h3>Background</h3><p>Most acute abdominal pain raises the suspension of infectious or inflammatory processes. The most obvious sign of acute intra-abdominal inflammation is frequently the identification of inflamed fat on the ultrasound. The study aimed to determine the specificity and sensitivity of focal hyperechogenic fat sign as a localizer of abdominal pathologies and to correlate findings with other imaging modalities as well as operative and pathological data.</p><h3>Results</h3><p>Seventy-six patients with abdominal pain have been examined by ultrasound. In 32 patients (42.1%), focal hyperechogenic fat sign was the only detected sign by ultrasound; however, it was associated with other findings in 44 patients (57.9%). In 16 patients (21.1%), echogenic fat represents the primary source of pathology, while in 59 patients (77.6%), underlying pathologies were detected, &amp; the echogenic fat was a secondary finding. The diagnostic performance of hyperechogenic fat signs shown in the US in detecting the cause of abdominal pain was true-positive sign in 75 cases &amp; false positive in one case. Its sensitivity is high. It was about 98.7%.</p><h3>Conclusion</h3><p>Our investigation demonstrated that focal hyperechoic mesenteric fat sign on abdominal ultrasound exhibited exceptionally high diagnostic performance, with a sensitivity of 100% and accuracy of 98.7% in detecting potential intra-abdominal pathologies. However, these findings necessitate validation through larger, multi-center prospective studies to establish broader clinical applicability.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-024-00593-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143835556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative RSM analysis of CO2 laser and abrasive waterjet machining on Armox 500 T armored steel","authors":"M. M. Salem,&nbsp;Tamer Ismail,&nbsp;Y. M. Elattar,&nbsp;A. M. El-Wardany,&nbsp;Ahmed Salama","doi":"10.1186/s43088-025-00617-x","DOIUrl":"10.1186/s43088-025-00617-x","url":null,"abstract":"<div><p>This study employs response surface methodology (RSM) to model and analyze the effects of abrasive waterjet (AWJ) and CO₂ laser cutting on the surface roughness of ARMOX 500 T armor-grade steel (7.6 mm thick). A comprehensive investigation was conducted to evaluate the influence of key AWJ process parameters, including cutting speed, water jet pressure, stand-off distance, and abrasive flow rate. Similarly, for CO₂ laser cutting, the effects of laser power, assist gas pressure, focus plane location, and cutting speed were examined. Analysis of variance (ANOVA) and process capability statistical techniques were utilized to assess the significance and reliability of the results. The findings indicate that CO₂ laser cutting achieves a lower surface roughness compared to AWJ, suggesting its superior capability for applications requiring high-quality surface finishes. These insights contribute to optimizing cutting processes for high-strength steel components, enhancing efficiency and precision in industrial applications.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-025-00617-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143809241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural diversity and biological activities of naturally derived bafilomycins from actinomycetes","authors":"Aileen Bayot Custodio,&nbsp;John Edward Ico Zapater,&nbsp;Edwin Plata Alcantara","doi":"10.1186/s43088-025-00622-0","DOIUrl":"10.1186/s43088-025-00622-0","url":null,"abstract":"<div><h3>Background</h3><p>Bafilomycins (Baf) are plecomacrolide antibiotics characterized by a 16-membered macrocyclic lactone containing two sets of conjugated dienes linked by a 1,3-dimethyl propan-2-ol (C₃) spacer to an unusually folded tetrahydropyran ring. Bafs are known for their vacuolar H⁺-adenosine triphosphatase (V-ATPase) inhibitory activity with potential therapeutic applications as antivirals (against, e.g., Severe acute respiratory syndrome coronavirus 2, Zika virus, rhinovirus), anti-tumor agents (against, e.g., osteosarcoma, various cancer cell lines), and antiparasitic (against e.g. nematodes) among others.</p><h3>Main body</h3><p>Since the discovery of the first Bafs in 1983, numerous Baf derivatives with varying biological activities have been isolated from Actinomycetales organisms, primarily <i>Streptomyces</i> and <i>Kitasatospora</i>. Variations in the Baf structure, particularly in the macrocyclic lactone and tetrahydropyran rings, have led to different Baf derivatives with various levels of biological activities and targets.</p><h3>Short conclusion</h3><p>This paper reviews the naturally derived Bafs from actinomycetes from 1983 to 2024, their structural diversity, and their biological activities. It also highlights the reported differences in Baf bioactivities with changes in structure. Furthermore, this paper classified Bafs based on structural variations relative to Baf A1, specifically: (1) substitution and (2) dehydration of residues; (3) addition of cyclic rings; and (4) tetrahydropyran ring opening. This paper hopes to shed light on underexplored Baf derivatives with more diverse and potent bioactivities.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-025-00622-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143801218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An eye surgery corneal nonlinear model: mathematical analysis and simulation via Dickson polynomials series","authors":"Atallah El-Shenawy,&nbsp;Mohammad Izadi,&nbsp;Mahmoud Abd El-Hady","doi":"10.1186/s43088-025-00616-y","DOIUrl":"10.1186/s43088-025-00616-y","url":null,"abstract":"<div><p>The paper offers an extensive mathematical study and simulation of a nonlinear corneal model pertinent to eye surgery, designed to tackle the intricacies of corneal behavior under diverse surgical situations. We utilize the Dickson polynomial series as a fundamental tool and use the benefits of the Dickson operational matrices collocation approach to establish a resilient solution framework. This method not only streamlines the computational procedure but also improves the precision of outcomes. Utilizing Dickson polynomials in our corneal nonlinear model represents a substantial advancement compared to conventional computational methods. Their unique properties provide a robust framework for accurately capturing the complex behaviors of the cornea during surgery. This results in enhanced computational efficiency, improved accuracy, and faster convergence rates compared to conventional techniques. The convergence analysis shown here illustrates the efficacy of our approach while verifying its speedy convergence to the accurate solution. Additionally, we present a comparative analysis with relevant computational techniques, demonstrating that our suggested approach delivers enhanced accuracy and efficiency. The results highlight the promise of the Dickson polynomial series in enhancing computational models in ophthalmology, facilitating future study and applications in eye surgical contexts.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-025-00616-y","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143793260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancing pancreatic cancer management: the role of artificial intelligence in diagnosis and therapy","authors":"Dilber Uzun Ozsahin,&nbsp;Natacha Usanase,&nbsp;Ilker Ozsahin","doi":"10.1186/s43088-025-00610-4","DOIUrl":"10.1186/s43088-025-00610-4","url":null,"abstract":"<div><h3>Background</h3><p>Pancreatic cancer is the deadliest form of cancer with a low survival rate due to its late diagnosis. Hence, early detection and swift intervention are very crucial for its management. However, the current diagnostic markers lack sufficient precision, and the effectiveness of treatment options remains imprecise, emphasizing the need for more advanced approaches.</p><h3>Main body</h3><p>Artificial intelligence (AI) technology enables rapid detection of high-risk groups for pancreatic cancer using various techniques such as medical imaging, pathological examination, biomarkers, and other methods, facilitating early detection of pancreatic cancer. Simultaneously, AI algorithms may also be used to forecast the duration of survival, the likelihood of recurrence, the cancer metastasis, and the response to treatment, all of which can impact the prognosis. Moreover, AI is applied in handling cancer cases in oncology departments, pancreatic cancer in particular, and creating computer-assisted diagnostic systems.</p><h3>Conclusion</h3><p>The end-to-end application of AI in pancreatic cancer management calls for multidisciplinary collaboration among doctors, laboratory scientists, data analysts, and engineers. Despite its limitations, its powerful computational capabilities will soon be crucial for combating pancreatic cancer and other health conditions.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-025-00610-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143793259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular characterization of chicken anemia virus and evaluation of maternally derived antibodies defense against commercially available vaccines","authors":"Amal R. Abdel Aziz,&nbsp;Kh. M. Elbayoumi,&nbsp;Sanaa S. A. Awad,&nbsp;Samah M. Mosad,&nbsp;Kamel I. Abou El-Azm","doi":"10.1186/s43088-025-00620-2","DOIUrl":"10.1186/s43088-025-00620-2","url":null,"abstract":"<div><h3>Background</h3><p>Chicken infectious anemia is a young chicken’s infection caused by a single-strand DNA <i>gyrovirus</i> and marked by aplastic anemia, lymphoid organs atrophy, and immunosuppression, causing severe financial losses to the poultry production. The prevalence of chicken anemia virus (CAV) in 25% of Egyptian governorates (Dakahlia, Gharbia, Monufia, Ismailia, Sinai, Damietta, Al sharqiya) from 2021 to 2023 was investigated. The protective efficacy of maternally derived antibodies was assessed in one-day-old chicks against three CAV vaccines via different exposure routes (intramuscular, drinking water, or contact) to mimic field strains.</p><h3>Results</h3><p>Out of 98 flocks examined from 2021 to 2023, 32.65% tested positive for chicken anemia. The infection rate was 25.92% in broiler and 40.91% in unvaccinated breeder flocks. VP1 and VP3 genes sequencing and phylogenetic analysis of four chicken anemia isolates revealed that three strains (EGY-1, EGY-9, and EGY-10) closely resemble most Egyptian field strains and vaccinal strains. In contrast, one strain (EGY-5) showed lineage with some Asian and Egyptian strains. VP1 and VP3 genes amino acid substitutions, including M70I in (EGY-5, EGY-9, and EGY-10) and N78T in (EGY-1 and EGY-10), have been recorded, marking the first recorded alterations in these genes compared to vaccinal strains and other Egyptian isolates. In an experiment simulating the effects of field strains, three vaccinal strains (Cux-1, Del-Ros, and 26P4) were administered either intramuscularly or via drinking water to 120 one-day-old commercial chicks with maternally derived antibodies (4882 mean antibody titers). However, these vaccines did not provide complete protection against infection with chicken anemia vaccine viruses, resulting in histopathological alterations, body weights, and chicken viability.</p><h3>Conclusion</h3><p>This research enhances the understanding of chicken anemia molecular characterization in Egypt and its implications for future genetic evolution studies. Further studies are necessary to determine the maternal antibody levels required for complete protection against CAV.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-025-00620-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143769877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular detection of bacteria isolated from polluted environment and screening their ability to produce extracellular biopolymer flocculants","authors":"Hawraa Qays Al-assdy,&nbsp;Wijdan Hussein Al-Tamimi,&nbsp;Asia Fadhile Almansoory","doi":"10.1186/s43088-025-00621-1","DOIUrl":"10.1186/s43088-025-00621-1","url":null,"abstract":"<div><h3>Background</h3><p>Microorganism bioflocculants are the large molecules released by microbes during growth and lysis. Bioflocculants are used in remediation wastewater and are thought to be more environmentally friendly. In the present study, 16 bacteria were isolated from hydrocarbons contaminated soil, sludge, and wastewater from different locations (Washing and lubrication stations of Zubair, Qurna, and Jazira, Beach of Shatt Al -Arab, and Al-Shuaiba Refinery) in Basrah city, south of Iraq. The isolates were identified by <i>16S rDNA</i> gene sequencing analysis. All isolated bacteria were subjected to a flocculants production test using a mineral salt medium. Bioflocculant activity was determined using kaolin clay and enhanced by addition cation (CaCl₂).</p><h3>Result</h3><p>The results showed that bacterial isolates were under 10 genera (<i>Alishewanella, Stutzerimonas, Pseudomonas, Bacillus, Pantoe, Acinetobacter, Escherichia, Exiguobacterium, Franconibacter, Lysinibacillus</i>), and nine isolates were recorded as new strains. Besides, the Phylogenetic tree was constructed to evaluate their close relationship and evolution between them. <i>Alishewanella</i> sp. was the most diverse and dominant genus among sixteen isolated bacteria. The isolates <i>Shewanella chilikensis, Exiguobacterium profundum,</i> and <i>Alishewanella jeotgali</i> were the most effective producing bioflocculant, where the flocculation activity recorded at 92.40%, 92.25%, and 91.65%, respectively. The ion Ca²⁺ removes most large molecules and reduces solution absorption from 1.918 (kaolin clay) to 1.258.</p><h3>Conclusion</h3><p>The contaminated environments harbor a diverse bioflocculant producing bacteria. The capacity of bacterial genera to produce bioflocculants varies, requiring the selection of optimal bacteria for bioflocculant production and their application in water treatment as effective alternatives to synthetic flocculants. The considerable flocculation activity seen suggests a potential for industrial applications. Moreover, more research on the process parameters is required to determine the possibility of large-scale production and to identify a compound responsible for flocculation activity.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-025-00621-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143761736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}