Gerald Zirintunda, John Kateregga, Sarah Nalule, Savino Biryomumaisho, Francis Omujal, James Okwee-Acai, Patrick Vudriko
{"title":"番木瓜和辣椒提取物与枸橼酸哌嗪和盐酸左旋咪唑对家禽蠕虫的治疗效果相当","authors":"Gerald Zirintunda, John Kateregga, Sarah Nalule, Savino Biryomumaisho, Francis Omujal, James Okwee-Acai, Patrick Vudriko","doi":"10.1186/s43088-025-00607-z","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>In rural smallholder poultry production systems, synthetic anthelmintic drugs are considered expensive and in some instances ineffective because of anthelmintic resistance. We report on the phytochemical properties and efficacy of crude extracts of <i>Carica papaya</i> L. and <i>Capsicum annuum</i> L. against helminth infections of chickens. The experiments that compared the extract action to piperazine and levamisole were carried out in Soroti District, Eastern Uganda.</p><h3>Method</h3><p>An experiment was set to evaluate efficacy of crude extracts of <i>C. papaya</i> and <i>C. annuum</i> against natural poultry helminths infections. Commercially available formulations of levamisole and piperazine were used to make a comparative efficacy study. Faecal egg count reduction (FECR) tests were used to measure efficacy of the treatments.</p><h3>Results</h3><p>On gas chromatograph mass spectrometry (GC–MS) analysis of CPLa showed, vitamin C (42%), sterols (13%) and Triterpenoids (6%). CPLe contained lipids (45.04%), pyranones (20.3%), diterpenoids (4.9%), triterpenoids (3.5%), phenolics (3.1%), glycosides (2.2%) and steroids (1.4%). GC–MS analysis of CAFa gave lipids (45.04%), alkanes (27.7%) and alkaloids (8.2%). CAFe showed lipids (50.16%), alkaloids (22.73%), glycosides (3.61%) and pyranones (3.55%). In the in vitro assays, 0.08 g/ml of each of the extracts caused motility inhibition of more than 50% of adult <i>A. galli</i> after 5 h. The ranking of the in vivo average FECR was levamisole hydrochloride > CPLa > CAFa > CAFe > CPLe > piperazine citrate with the percentage reductions of 98.67 ± 2.309, 97.67 ± 2.517, 79.67 ± 1.528, 76.33 ± 1.528, 54.00 ± 2.00, 35.67 ± 2.082, respectively.</p><h3>Conclusion</h3><p>The GC–MS analysis of the analysed plants shows presence of terpenoids, phenolics and alkaloids which are known for anthelmintic action. All the extracts caused higher FECR than piperazine. The presence of vitamin C in CPLa made it the best extract. Combinations of anthelmintics with vitamin C are recommended and toxicological studies of extracts.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"14 1","pages":""},"PeriodicalIF":2.5000,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-025-00607-z","citationCount":"0","resultStr":"{\"title\":\"Extracts of Carica papaya L. and Capsicum annuum L. showed comparable efficacy to piperazine citrate and levamisole hydrochloride in treatment of poultry helminths\",\"authors\":\"Gerald Zirintunda, John Kateregga, Sarah Nalule, Savino Biryomumaisho, Francis Omujal, James Okwee-Acai, Patrick Vudriko\",\"doi\":\"10.1186/s43088-025-00607-z\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>In rural smallholder poultry production systems, synthetic anthelmintic drugs are considered expensive and in some instances ineffective because of anthelmintic resistance. We report on the phytochemical properties and efficacy of crude extracts of <i>Carica papaya</i> L. and <i>Capsicum annuum</i> L. against helminth infections of chickens. The experiments that compared the extract action to piperazine and levamisole were carried out in Soroti District, Eastern Uganda.</p><h3>Method</h3><p>An experiment was set to evaluate efficacy of crude extracts of <i>C. papaya</i> and <i>C. annuum</i> against natural poultry helminths infections. Commercially available formulations of levamisole and piperazine were used to make a comparative efficacy study. Faecal egg count reduction (FECR) tests were used to measure efficacy of the treatments.</p><h3>Results</h3><p>On gas chromatograph mass spectrometry (GC–MS) analysis of CPLa showed, vitamin C (42%), sterols (13%) and Triterpenoids (6%). CPLe contained lipids (45.04%), pyranones (20.3%), diterpenoids (4.9%), triterpenoids (3.5%), phenolics (3.1%), glycosides (2.2%) and steroids (1.4%). GC–MS analysis of CAFa gave lipids (45.04%), alkanes (27.7%) and alkaloids (8.2%). CAFe showed lipids (50.16%), alkaloids (22.73%), glycosides (3.61%) and pyranones (3.55%). In the in vitro assays, 0.08 g/ml of each of the extracts caused motility inhibition of more than 50% of adult <i>A. galli</i> after 5 h. The ranking of the in vivo average FECR was levamisole hydrochloride > CPLa > CAFa > CAFe > CPLe > piperazine citrate with the percentage reductions of 98.67 ± 2.309, 97.67 ± 2.517, 79.67 ± 1.528, 76.33 ± 1.528, 54.00 ± 2.00, 35.67 ± 2.082, respectively.</p><h3>Conclusion</h3><p>The GC–MS analysis of the analysed plants shows presence of terpenoids, phenolics and alkaloids which are known for anthelmintic action. All the extracts caused higher FECR than piperazine. The presence of vitamin C in CPLa made it the best extract. 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Extracts of Carica papaya L. and Capsicum annuum L. showed comparable efficacy to piperazine citrate and levamisole hydrochloride in treatment of poultry helminths
Background
In rural smallholder poultry production systems, synthetic anthelmintic drugs are considered expensive and in some instances ineffective because of anthelmintic resistance. We report on the phytochemical properties and efficacy of crude extracts of Carica papaya L. and Capsicum annuum L. against helminth infections of chickens. The experiments that compared the extract action to piperazine and levamisole were carried out in Soroti District, Eastern Uganda.
Method
An experiment was set to evaluate efficacy of crude extracts of C. papaya and C. annuum against natural poultry helminths infections. Commercially available formulations of levamisole and piperazine were used to make a comparative efficacy study. Faecal egg count reduction (FECR) tests were used to measure efficacy of the treatments.
Results
On gas chromatograph mass spectrometry (GC–MS) analysis of CPLa showed, vitamin C (42%), sterols (13%) and Triterpenoids (6%). CPLe contained lipids (45.04%), pyranones (20.3%), diterpenoids (4.9%), triterpenoids (3.5%), phenolics (3.1%), glycosides (2.2%) and steroids (1.4%). GC–MS analysis of CAFa gave lipids (45.04%), alkanes (27.7%) and alkaloids (8.2%). CAFe showed lipids (50.16%), alkaloids (22.73%), glycosides (3.61%) and pyranones (3.55%). In the in vitro assays, 0.08 g/ml of each of the extracts caused motility inhibition of more than 50% of adult A. galli after 5 h. The ranking of the in vivo average FECR was levamisole hydrochloride > CPLa > CAFa > CAFe > CPLe > piperazine citrate with the percentage reductions of 98.67 ± 2.309, 97.67 ± 2.517, 79.67 ± 1.528, 76.33 ± 1.528, 54.00 ± 2.00, 35.67 ± 2.082, respectively.
Conclusion
The GC–MS analysis of the analysed plants shows presence of terpenoids, phenolics and alkaloids which are known for anthelmintic action. All the extracts caused higher FECR than piperazine. The presence of vitamin C in CPLa made it the best extract. Combinations of anthelmintics with vitamin C are recommended and toxicological studies of extracts.
期刊介绍：
Beni-Suef University Journal of Basic and Applied Sciences (BJBAS) is a peer-reviewed, open-access journal. This journal welcomes submissions of original research, literature reviews, and editorials in its respected fields of fundamental science, applied science (with a particular focus on the fields of applied nanotechnology and biotechnology), medical sciences, pharmaceutical sciences, and engineering. The multidisciplinary aspects of the journal encourage global collaboration between researchers in multiple fields and provide cross-disciplinary dissemination of findings.
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