Applied Microscopy最新文献_第3页

Fractography of white charcoal reveals past fungal infection and embolism in the secondary xylem via SEM 白色木炭的断口形貌通过扫描电镜显示了次生木质部过去的真菌感染和栓塞

Applied Microscopy Pub Date : 2026-03-03 DOI: 10.1186/s42649-026-00126-w

Chul Jong Yoon, Ki Woo Kim

{"title":"Fractography of white charcoal reveals past fungal infection and embolism in the secondary xylem via SEM","authors":"Chul Jong Yoon, Ki Woo Kim","doi":"10.1186/s42649-026-00126-w","DOIUrl":"10.1186/s42649-026-00126-w","url":null,"abstract":"<div>\u0000 \u0000 <p>The internal structures of fractured white charcoal were investigated using a scanning electron microscope. The charcoal was fractured using a razor blade and hammer, gold-coated, and observed under the electron microscope. Both embolized and conductive vessels coexisted across the transverse surfaces of the fractured charcoal. Vessels were predominantly ellipsoidal, with an average diameter of approximately 250 μm. Embolized vessels exhibited membranous tyloses within their lumens. The presence of ring-porous wood, axial parenchyma cells, and xylem fibers within the secondary xylem implies that the white charcoal was produced from a <i>Quercus</i> species. Fungal hyphae were observed to proliferate, branch, and sporulate on the secondary cell walls of vessels. The conidiogenous cells and conidia closely resembled those of a fungal pathogen known to cause oak wilt disease in South Korea. Fungal hyphae were also found within the pits of the secondary cell walls. These observations suggest that the oak tree used for charcoal production in this study may have been diseased. These results indicate that wood structures are preserved through the white charcoal production process, allowing the observation of fungal structures within the host.</p>\u0000 </div>","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"56 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s42649-026-00126-w.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147336300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in EBSD-based approaches for quantifying slip activity in deformed Mg alloys 基于ebsd的变形镁合金滑移活度定量方法研究进展。

Applied Microscopy Pub Date : 2026-02-12 DOI: 10.1186/s42649-026-00124-y

Hafiz Muhammad Rehan Tariq, Tea-Sung Jun

{"title":"Advances in EBSD-based approaches for quantifying slip activity in deformed Mg alloys","authors":"Hafiz Muhammad Rehan Tariq, Tea-Sung Jun","doi":"10.1186/s42649-026-00124-y","DOIUrl":"10.1186/s42649-026-00124-y","url":null,"abstract":"<div><p>Plastic deformation in Mg alloys requires a full understanding of slip activity and intergranular interactions, which determine mechanical behavior and strain localization. Electron backscatter diffraction (EBSD) has emerged as a versatile technique to map crystallographic orientations, slip systems, and lattice rotations, permitting the systematic analysis of deformation mechanisms across polycrystalline aggregates. The coupling of EBSD with metrics including Schmid factors, ingrain misorientation axes, and slip-transfer criteria permits a quantitative assessment of slip compatibility and the role of grain boundaries in strain accommodation. Limitations related to conventional 2D surface characterization create a growing need for novel three-dimensional techniques that can accurately represent grain boundary geometry as well as complex intergranular deformation pathways. A focused review of such methodologies will compile current knowledge on these methods and their capabilities and limitations, guiding future investigations toward a deeper understanding of microstructure-mechanics relationships in Mg alloys.</p></div>","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"56 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12894533/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146163278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antonie van Leeuwenhoek and the dawn of microscopic observation: a narrative review from Delft’s lens to the modern microscope 安东尼·范·列文虎克与显微镜观察的曙光：从代尔夫特的镜头到现代显微镜的叙事回顾。

Applied Microscopy Pub Date : 2026-02-02 DOI: 10.1186/s42649-026-00123-z

Hyunjeong Lee, Im Joo Rhyu

{"title":"Antonie van Leeuwenhoek and the dawn of microscopic observation: a narrative review from Delft’s lens to the modern microscope","authors":"Hyunjeong Lee, Im Joo Rhyu","doi":"10.1186/s42649-026-00123-z","DOIUrl":"10.1186/s42649-026-00123-z","url":null,"abstract":"<div><p>Antonie van Leeuwenhoek (1632–1723) transformed observation into science through the power of a single handmade lens. His work emerged from the visual culture of seventeenth-century Delft, where craftsmanship, optics, and artistic precision intersected. While Robert Hooke’s compound microscope introduced the idea of microscopic visualization, Leeuwenhoek’s single-lens instruments achieved far superior magnification and resolution by minimizing optical interfaces. Using these deceptively simple devices, he documented the first observations of free-living microorganisms, fungal hyphae, red blood cells, capillary flow, oral bacteria, and spermatozoa in more than two hundred letters to the Royal Society of London.</p><p>But his investigations reached far beyond microbiology. Leeuwenhoek also examined the barbed structure of the bee sting, the ordered vessels of ash wood, and the geometric microstructure of crystals and salts—demonstrating that hidden organization pervades both living and non-living matter. These studies established microscopy as a universal investigative tool, capable of unifying biology, medicine, botany, and early materials science under a single optical principle.</p><p>Leeuwenhoek’s work marks one of the earliest examples of how rigorous observation can redefine scientific domains. His use of a home-crafted single lens created an empirical foundation for biological microscopy that persists to this day. The legacy of his minimalist optical design also survives in the digital age: modern clip-on smartphone microscopes and paper-based platforms such as the Foldscope reproduce the same single-lens principle through micro-optics mounted directly onto digital sensors.</p><p>Three and a half centuries later, his work continues to remind us that new worlds do not emerge from new theories alone, but from new ways of seeing.</p></div>","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"56 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12862032/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146099649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effects of nanopillar and nanopit arrays on the morphology and osteogenic differentiation of adipose-derived stem cells 纳米柱和纳米颗粒阵列对脂肪干细胞形态和成骨分化的影响

Applied Microscopy Pub Date : 2026-01-24 DOI: 10.1186/s42649-026-00122-0

Jihun Kang, Young-Shik Yun, Eun-Hye Kang, Jihye Lee, Deok-Jin Jeon, Seungmuk Ji, Yong-Oock Kim, In-Sik Yun, Jong-Souk Yeo

{"title":"The effects of nanopillar and nanopit arrays on the morphology and osteogenic differentiation of adipose-derived stem cells","authors":"Jihun Kang, Young-Shik Yun, Eun-Hye Kang, Jihye Lee, Deok-Jin Jeon, Seungmuk Ji, Yong-Oock Kim, In-Sik Yun, Jong-Souk Yeo","doi":"10.1186/s42649-026-00122-0","DOIUrl":"10.1186/s42649-026-00122-0","url":null,"abstract":"<div>\u0000 \u0000 <p>Nanotopographic control of cell behavior offers great potential in designing biomimetic scaffolds for cell therapy. However, the behavior of cells on different nanotopographies is not fully understood. In this study, we investigated the effect of nanostructures on human adipose-derived stem cells (ASCs) by directly comparing nanopillar and nanopit arrays. Morphological changes, cell viability and early osteogenic differentiation of ASCs have been analyzed on the nanostructures. Nanopit arrays were found to increase cell areas and promote early osteogenic differentiation more than nanopillar arrays. Analysis of focal adhesion (FA) formation indicated a larger increase in total area as well as the number of FAs during cell spreading on nanopit arrays. The maturation of FA is related to cellular traction forces, which are known to stimulate osteogenic induction through the RhoA-ROCK pathway. We conclude that ASCs can spread more on the nanopit array than on the nanopillar array due to the presence of continuous adhesive paths on the nanopit array, which is associated with increased expression of RUNX2 as an early osteogenic marker. Our results suggest that a connected path in nanopit arrays plays a critical role in controlling stem cell behavior compared to nanopillar arrays. A comparative understanding of nanostructures can provide a guideline for designing an artificial substrate for osteogenesis and tissue engineering. </p>\u0000 </div>","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"56 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s42649-026-00122-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146027394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effective elimination of Moiré fringe at the nanoprecipitate/matrix interface 有效消除纳米沉淀/基体界面处的莫尔条纹

Applied Microscopy Pub Date : 2026-01-07 DOI: 10.1186/s42649-025-00121-7

Yoon-Uk Heo, Dongwon Lee, T. T. T. Trang, Changwan Hong

{"title":"Effective elimination of Moiré fringe at the nanoprecipitate/matrix interface","authors":"Yoon-Uk Heo, Dongwon Lee, T. T. T. Trang, Changwan Hong","doi":"10.1186/s42649-025-00121-7","DOIUrl":"10.1186/s42649-025-00121-7","url":null,"abstract":"<div><p>The effective method for eliminating the Moiré fringes in the MC nanoprecipitates in the austenite matrix was studied. Considering the dynamic diffraction between the matrix and precipitate, the spots originating from the Moiré pattern were selected in the fast Fourier transform (FFT) pattern. The Moiré fringe image was extracted by performing inverse FFT(IFFT) of the selected spots. Subtracting the Moiré fringe contribution from the original image intensity reveals the veiled lattice fringes of MC carbide in the austenite matrix. The image was compared and discussed with the IFFT image of the direct selection of the matrix + MC FFT patterns. The suggested method was applied to strain measurement in the MC carbide-containing area using the geometrical phase analysis. Compared with the result obtained from the raw image containing Moiré fringes, the applied method shows artifact-free strain distribution in the MC carbide. The process was applied to the inclined twin interface in BCC steel. The lattice fringe in the overlapping area shows a more detailed lattice feature. This novel study sheds light on the veiled crystal structure and strain distribution under various conditions where more than two lattice fringes overlap.</p></div>","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"56 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s42649-025-00121-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145904513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlative x-ray microscopy and transmission electron microscopy for ultrastructural analysis of zebrafish tissue 相关x射线显微镜及透射电镜对斑马鱼组织超微结构的分析

Applied Microscopy Pub Date : 2025-12-27 DOI: 10.1186/s42649-025-00120-8

Sun-Yeong Gwon, MinKi Choi, Ji Young Mun

引用次数: 0

From a clinical case to a general methodology to analyze prosthetic joint failure, by micro- and nano-characterization with SEM of intra-tissue wear debris 从临床案例到一般方法分析假体关节失效，通过微观和纳米表征组织内磨损碎片的扫描电镜。

Applied Microscopy Pub Date : 2025-12-15 DOI: 10.1186/s42649-025-00118-2

Stefania Tarter, Lorena Maines, Giandomenico Nollo, Marco Molinari, Stefano Gialanella

{"title":"From a clinical case to a general methodology to analyze prosthetic joint failure, by micro- and nano-characterization with SEM of intra-tissue wear debris","authors":"Stefania Tarter, Lorena Maines, Giandomenico Nollo, Marco Molinari, Stefano Gialanella","doi":"10.1186/s42649-025-00118-2","DOIUrl":"10.1186/s42649-025-00118-2","url":null,"abstract":"<div><p>Release of prosthesis debris at the tissue-implant interface is a major cause of aseptic loosening, a phenomenon that requires premature replacement of the prosthesis. The main objective of this paper is to propose a step-structured <i>modus operandi</i> for a reliable scanning electron microscopy (SEM) analysis of debris released from prostheses into the surrounding tissues. Following a proven methodology for the analysis of this wear debris would allow research and hospital laboratories to reduce time and obtain results associated with a common protocol and consequently, more comparable results. For developing the methodology, we chose the clinical case of a hip prosthetic revision, in which a Cr-Co head misalignment caused the wearing out of the polyethylene acetabular insert and a partial wear of the Ti-6Al-4 V acetabular cup. Samples of periprosthetic tissues, after being partially digested in a KOH basic solution, were investigated in vivo and in situ with SEM observations and Energy Dispersive X-rays Spectroscopy (EDXS) analyses. Although developed from a specific case study, this methodology is compatible and applicable to other standard cases as well. Regarding the set of samples we selected, a complete set of micro- and nano-structural analysis, compositional spectra and high-resolution images have been acquired, showing the morphology of the debris involved, and the agglomeration phenomena occurring in the tissue. The proposed protocol complements previous studies on tribological phenomena, underlying debris production at the tissue-prosthesis interface, best digestion techniques for fragment isolation, and nanotoxicology.</p></div>","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12705928/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145754692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of electron ptychography from algorithms, detectors to its applications 从算法、检测器到应用的电子印刷技术的发展。

Applied Microscopy Pub Date : 2025-12-03 DOI: 10.1186/s42649-025-00119-1

Wonwoo Suh, Jeewon B. Choi, Keun-Yeol Park, Celesta S. Chang

引用次数: 0

Anatomy and histology of the olfactory organ of Korean amur goby Rhinogobius brunneus (Gobiiformes, Gobiidae) 韩国虾虎鱼（虾虎目，虾虎科）嗅觉器官的解剖与组织学

Applied Microscopy Pub Date : 2025-11-29 DOI: 10.1186/s42649-025-00116-4

Hyun Tae Kim

{"title":"Anatomy and histology of the olfactory organ of Korean amur goby Rhinogobius brunneus (Gobiiformes, Gobiidae)","authors":"Hyun Tae Kim","doi":"10.1186/s42649-025-00116-4","DOIUrl":"10.1186/s42649-025-00116-4","url":null,"abstract":"<div>\u0000 \u0000 <p>This study investigated the anatomy and histology of the olfactory organ of the Korean amur goby <i>Rhinogobius brunneus</i> from Jeonjucheon stream. This species lives in shallow, stagnant, and intermittently low-oxygenated streams, reservoirs, and ponds affected by seasonal rainfall. Anatomically, its olfactory organ consisted of a short tubular anterior nostril, a posterior nostril, a single longitudinal lamella, and two accessory nasal sacs (ethmoidal and lacrymal sacs). Its single lamella structure resembles other gobiid fishes with a simplified olfactory surface. Histologically, the sensory epithelium comprised olfactory receptor neurons, supporting cells, and basal cells, while the non-sensory epithelium contained stratified epithelial cells, basal cells, and mucous cells. In particular, the mucous cells stained intensely red with Hematoxylin and eosin and Masson’s trichrome, indicating proteinaceous granular mucins rich in glycoproteins; such secretions likely protect the epithelium against particles, toxins, and low-oxygenated freshwater. Overall, the goby’s olfactory organ exhibits traits uniquely adapted to stagnant and hypoxic conditions.</p>\u0000 </div>","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s42649-025-00116-4.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145613111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electron diffraction and dark-field TEM for structural analysis of 2D van der Waals materials 二维范德华材料的电子衍射和暗场透射电镜结构分析。

Applied Microscopy Pub Date : 2025-11-11 DOI: 10.1186/s42649-025-00117-3

Byunghyun Kim, Daesung Park, Siwon Jeong, Hyobin Yoo

{"title":"Electron diffraction and dark-field TEM for structural analysis of 2D van der Waals materials","authors":"Byunghyun Kim, Daesung Park, Siwon Jeong, Hyobin Yoo","doi":"10.1186/s42649-025-00117-3","DOIUrl":"10.1186/s42649-025-00117-3","url":null,"abstract":"<div><p>Two-dimensional (2D) van der Waals materials possess structural degrees of freedom that set them apart from conventional bulk crystals and strongly influence their physical properties. Such freedom, enabled by the weak interlayer bonding, permits stacking, twisting, and lateral sliding of layers, leading to structural variations such as out-of-plane corrugations, layer-number–dependent electronic and optical responses, and interlayer registry variations that produce stacking domains with distinct functionalities. Capturing and understanding these variations is essential for linking structure to function. Transmission electron microscopy (TEM) offers complementary approaches for this purpose: electron diffraction provides quantitative crystallographic fingerprints, while dark-field (DF) imaging translates selected diffraction information into spatial maps of local structure. When combined, these techniques can resolve complex structural modulations across multiple length scales and under diverse experimental conditions. Recent advances have extended diffraction and DF imaging into in-situ and operando regimes, enabling real-time observation of domain reconfiguration, phase transitions, and polarization switching under external stimuli. This review discusses how these methods are applied to 2D van der Waals materials to reveal structural degrees of freedom and illustrates their unique capability to directly connect structural evolution to functional behavior.</p></div>","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://appmicro.springeropen.com/counter/pdf/10.1186/s42649-025-00117-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145487319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0