Applied Microscopy最新文献_第3页

Changes of synaptic vesicles in three-dimensional synapse models by treatment with umbelliferone in scopolamine-induced hippocampal injury model 在东莨菪碱诱导的海马损伤模型中使用伞形酮治疗三维突触模型中突触小泡的变化

Applied Microscopy Pub Date : 2024-01-23 DOI: 10.1186/s42649-024-00095-y

Ga-Young Choi, Eunyoung Moon, Hyosung Choi, Hee-Seok Kweon

引用次数: 0

A simple and rapid preparation of smooth muscle myosin 2 for the electron microscopic analysis 用于电子显微镜分析的平滑肌肌球蛋白 2 的简单快速制备方法。

Applied Microscopy Pub Date : 2024-01-02 DOI: 10.1186/s42649-023-00094-5

Anahita Vispi Bharda, Hyun Suk Jung

{"title":"A simple and rapid preparation of smooth muscle myosin 2 for the electron microscopic analysis","authors":"Anahita Vispi Bharda, Hyun Suk Jung","doi":"10.1186/s42649-023-00094-5","DOIUrl":"10.1186/s42649-023-00094-5","url":null,"abstract":"<div><p>There has been an increase in the demand for purified protein as a result of recent developments in the structural biology of myosin 2. Although promising, current practices in myosin purification are usually time-consuming and cumbersome. The reported increased actin to myosin ratio in smooth muscles adds to the complexity of the purification process. Present study outlines a streamlined approach to isolate smooth muscle myosin 2 molecules from actomyosin suspension of chicken gizzard tissues. The procedure entails treating actomyosin for a brief period with actin-binding peptide phalloidin, followed by co-sedimentation and short column size exclusion chromatography. Typical myosin molecule with heavy and light chains and approximately 95% purity was examined using gel electrophoresis. Negative staining electron microscopy and image processing showed intact 10S myosin 2 molecules, proving that phalloidin is effective at eliminating majority of actin in the form of F-actin without dramatic alteration in the structure of myosin. The entire purification discussed here can be completed in a few hours, and further analysis can be done the same day. Thus, by offering quick and fresh supplies of native myosin molecules suited for structural research, specially cryo-electron microscopy, this innovative approach can be adapted to get around the drawbacks of time-intensive myosin purifying processes.</p></div>","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"54 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://appmicro.springeropen.com/counter/pdf/10.1186/s42649-023-00094-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139072988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histology and morphometry of the skin of purple spaghetti-eel Moringua raitaborua (Anguilliformes, Moringuidae) 紫鳗（Anguilliformes，Moringuaraitaborua）皮的组织学和形态计量学。

Applied Microscopy Pub Date : 2023-10-31 DOI: 10.1186/s42649-023-00093-6

Hyun-Tae Kim

引用次数: 0

Three-dimensional reconstruction of Y-IrNi rhombic dodecahedron nanoframe by STEM/EDS tomography Y-IrNi菱形十二面体纳米框架的STEM/EDS断层扫描三维重建。

Applied Microscopy Pub Date : 2023-09-21 DOI: 10.1186/s42649-023-00092-7

Taekyung Kim, Yongsang Lee, Yongju Hong, Kwangyeol Lee, Hionsuck Baik

{"title":"Three-dimensional reconstruction of Y-IrNi rhombic dodecahedron nanoframe by STEM/EDS tomography","authors":"Taekyung Kim, Yongsang Lee, Yongju Hong, Kwangyeol Lee, Hionsuck Baik","doi":"10.1186/s42649-023-00092-7","DOIUrl":"10.1186/s42649-023-00092-7","url":null,"abstract":"<div><p>The structural analysis of nanocrystals via transmission electron microscopy (TEM) is a valuable technique for the material science field. Recently, two-dimensional images by scanning TEM (STEM) and energy-dispersive X-ray spectroscopy (EDS) have successfully extended to three-dimensional (3D) imaging by tomography. However, despite improving TEM instruments and measurement techniques, detector shadowing, the missing-wedge problem, X-ray absorption effects, etc., significant challenges still remain; therefore, the various required corrections should be considered and applied when performing quantitative tomography. Nonetheless, this 3D reconstruction technique can facilitate active site analysis and the development of nanocatalyst systems, such as water electrolysis and fuel cell. Herein, we present a 3D reconstruction technique to obtain tomograms of IrNi rhombic dodecahedral nanoframes (IrNi-RFs) from STEM and EDS images by applying simultaneous iterative reconstruction technique and total variation minimization algorithms. From characterizing the morphology and spatial chemical composition of the Ir and Ni atoms in the nanoframes, we were able to infer the origin of the physical and catalytic durability of IrNi-RFs. Also, by calculating the surface area and volume of the 3D reconstructed model, we were able to quantify the Ir-to-Ni composition ratio and compare it to the EDS measurement result.</p></div>","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"53 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511395/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41093214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Standardizing HER2 immunohistochemistry assessment: calibration of color and intensity variation in whole slide imaging caused by staining and scanning 标准化HER2免疫组织化学评估:校正染色和扫描引起的全片成像的颜色和强度变化

Applied Microscopy Pub Date : 2023-09-14 DOI: 10.1186/s42649-023-00091-8

Chie Ohnishi, Takashi Ohnishi, Peter Ntiamoah, Dara S. Ross, Masahiro Yamaguchi, Yukako Yagi

{"title":"Standardizing HER2 immunohistochemistry assessment: calibration of color and intensity variation in whole slide imaging caused by staining and scanning","authors":"Chie Ohnishi, Takashi Ohnishi, Peter Ntiamoah, Dara S. Ross, Masahiro Yamaguchi, Yukako Yagi","doi":"10.1186/s42649-023-00091-8","DOIUrl":"10.1186/s42649-023-00091-8","url":null,"abstract":"<div><p>In the evaluation of human epidermal growth factor receptor 2 (HER2) immunohistochemistry (IHC) — one of the standard biomarkers for breast cancer— visual assessment is laborious and subjective. Image analysis using whole slide image (WSI) could produce more consistent results; however, color variability in WSIs due to the choice of stain and scanning processes may impact image analysis. We therefore developed a calibration protocol to diminish the staining and scanning variations of WSI using two calibrator slides. The IHC calibrator slide (IHC-CS) contains peptide-coated microbeads with different concentrations. The color distribution obtained from the WSI of stained IHC-CS reflects the staining process and scanner characteristics. A color chart slide (CCS) is also useful for calibrating the color variation due to the scanner. The results of the automated HER2 assessment were compared to confirm the effectiveness of two calibration slides. The IHC-CS and HER2 breast cancer cases were stained on different days. All stained slides and CCS were digitized by two different WSI scanners. Results revealed 100% concordance between automated evaluation and the pathologist’s assessment with both the scanner and staining calibration. The proposed method may enable consistent evaluation of HER2.</p></div>","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"53 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10499734/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10260046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-obesity effects of yuja (Citrus junos Sieb ex Tanaka) pomace extract fermented with lactic acid bacteria on the hepatocytes and epididymal fat tissue of rats 乳酸菌发酵玉子渣提取物对大鼠肝细胞和附睾脂肪组织的抗肥胖作用

Applied Microscopy Pub Date : 2023-08-09 DOI: 10.1186/s42649-023-00090-9

Han-Na Chu, Haeng-Ran Kim, Kyeong-A. Jang, Yu-Jin Hwang, Jeong-Sang Kim

引用次数: 0

Fine structural characteristics of the chorionic microspheres on the egg surface of the orb web spider Trichonephila clavata 球蛛卵表面绒毛膜微球的精细结构特征

Applied Microscopy Pub Date : 2023-07-18 DOI: 10.1186/s42649-023-00087-4

Seung-Min Lee, Myung-Jin Moon

{"title":"Fine structural characteristics of the chorionic microspheres on the egg surface of the orb web spider Trichonephila clavata","authors":"Seung-Min Lee, Myung-Jin Moon","doi":"10.1186/s42649-023-00087-4","DOIUrl":"10.1186/s42649-023-00087-4","url":null,"abstract":"<div><p>The eggs laid by the orb web spider <i>Trichonephila clavata</i> must overwinter in bitterly freezing and dry conditions before hatching, but there does not seem to be any protection like a compact silk case covering the entire eggmass. Instead, the surface of the eggmass is completely coated with a milky coating called chorionic microspheres (CM). Therefore, we investigated the fine structural characteristics of CM to demonstrate their ecological importance. Although the diameter of CM in outer eggmass exhibits a significant variation, the chorionic surface is coated with a single layer of CM, characterized by a consistent diameter of approximately 2.3 µm. The surface structure of aggregated CM shows short papillary projections demonstrating segmental adhesion of mucous components. CM is insoluble in water but partially soluble in anhydrous ethanol, and its spherical structure is completely decomposed by hexafluoroisopropanol (HFIP), a strong organic solvent. Since our fine structural observations clearly show that CM is not derived from vitellogenic or choriogenetic processes, the CM adhesive coatings during ovipositional process appears to be equivalent to cocoon silk for various protective functions in silken eggcase.</p></div>","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"53 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://appmicro.springeropen.com/counter/pdf/10.1186/s42649-023-00087-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4722094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent progress in the role of grain boundaries in two-dimensional transition metal dichalcogenides studied using scanning tunneling microscopy/spectroscopy 扫描隧道显微镜/光谱学研究二维过渡金属二硫族化合物中晶界作用的最新进展

Applied Microscopy Pub Date : 2023-07-17 DOI: 10.1186/s42649-023-00088-3

Hyo Won Kim

引用次数: 0

Therapeutic treatments for diabetes mellitus-induced liver injury by regulating oxidative stress and inflammation 通过调节氧化应激和炎症治疗糖尿病性肝损伤

Applied Microscopy Pub Date : 2023-07-10 DOI: 10.1186/s42649-023-00089-2

Chun-Sik Bae, Youngchan Lee, Taeho Ahn

引用次数: 2

New recorded diatoms in Holocene sediment cores from the Gonggeom-ji Wetland in Korea 韩国贡贡记湿地全新世沉积物岩心中新记录的硅藻

Applied Microscopy Pub Date : 2023-01-20 DOI: 10.1186/s42649-023-00086-5

Daeryul Kwon, Mirye Park, Hoil Lee, Jin-Young Lee, Sang Deuk Lee

引用次数: 0