Applied Microscopy最新文献_第2页

Histology and morphometry of the skin of purple spaghetti-eel Moringua raitaborua (Anguilliformes, Moringuidae) 紫鳗（Anguilliformes，Moringuaraitaborua）皮的组织学和形态计量学。

Applied Microscopy Pub Date : 2023-10-31 DOI: 10.1186/s42649-023-00093-6

Hyun-Tae Kim

引用次数: 0

Three-dimensional reconstruction of Y-IrNi rhombic dodecahedron nanoframe by STEM/EDS tomography Y-IrNi菱形十二面体纳米框架的STEM/EDS断层扫描三维重建。

Applied Microscopy Pub Date : 2023-09-21 DOI: 10.1186/s42649-023-00092-7

Taekyung Kim, Yongsang Lee, Yongju Hong, Kwangyeol Lee, Hionsuck Baik

{"title":"Three-dimensional reconstruction of Y-IrNi rhombic dodecahedron nanoframe by STEM/EDS tomography","authors":"Taekyung Kim, Yongsang Lee, Yongju Hong, Kwangyeol Lee, Hionsuck Baik","doi":"10.1186/s42649-023-00092-7","DOIUrl":"10.1186/s42649-023-00092-7","url":null,"abstract":"<div><p>The structural analysis of nanocrystals via transmission electron microscopy (TEM) is a valuable technique for the material science field. Recently, two-dimensional images by scanning TEM (STEM) and energy-dispersive X-ray spectroscopy (EDS) have successfully extended to three-dimensional (3D) imaging by tomography. However, despite improving TEM instruments and measurement techniques, detector shadowing, the missing-wedge problem, X-ray absorption effects, etc., significant challenges still remain; therefore, the various required corrections should be considered and applied when performing quantitative tomography. Nonetheless, this 3D reconstruction technique can facilitate active site analysis and the development of nanocatalyst systems, such as water electrolysis and fuel cell. Herein, we present a 3D reconstruction technique to obtain tomograms of IrNi rhombic dodecahedral nanoframes (IrNi-RFs) from STEM and EDS images by applying simultaneous iterative reconstruction technique and total variation minimization algorithms. From characterizing the morphology and spatial chemical composition of the Ir and Ni atoms in the nanoframes, we were able to infer the origin of the physical and catalytic durability of IrNi-RFs. Also, by calculating the surface area and volume of the 3D reconstructed model, we were able to quantify the Ir-to-Ni composition ratio and compare it to the EDS measurement result.</p></div>","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"53 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511395/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41093214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Standardizing HER2 immunohistochemistry assessment: calibration of color and intensity variation in whole slide imaging caused by staining and scanning 标准化HER2免疫组织化学评估:校正染色和扫描引起的全片成像的颜色和强度变化

Applied Microscopy Pub Date : 2023-09-14 DOI: 10.1186/s42649-023-00091-8

Chie Ohnishi, Takashi Ohnishi, Peter Ntiamoah, Dara S. Ross, Masahiro Yamaguchi, Yukako Yagi

{"title":"Standardizing HER2 immunohistochemistry assessment: calibration of color and intensity variation in whole slide imaging caused by staining and scanning","authors":"Chie Ohnishi, Takashi Ohnishi, Peter Ntiamoah, Dara S. Ross, Masahiro Yamaguchi, Yukako Yagi","doi":"10.1186/s42649-023-00091-8","DOIUrl":"10.1186/s42649-023-00091-8","url":null,"abstract":"<div><p>In the evaluation of human epidermal growth factor receptor 2 (HER2) immunohistochemistry (IHC) — one of the standard biomarkers for breast cancer— visual assessment is laborious and subjective. Image analysis using whole slide image (WSI) could produce more consistent results; however, color variability in WSIs due to the choice of stain and scanning processes may impact image analysis. We therefore developed a calibration protocol to diminish the staining and scanning variations of WSI using two calibrator slides. The IHC calibrator slide (IHC-CS) contains peptide-coated microbeads with different concentrations. The color distribution obtained from the WSI of stained IHC-CS reflects the staining process and scanner characteristics. A color chart slide (CCS) is also useful for calibrating the color variation due to the scanner. The results of the automated HER2 assessment were compared to confirm the effectiveness of two calibration slides. The IHC-CS and HER2 breast cancer cases were stained on different days. All stained slides and CCS were digitized by two different WSI scanners. Results revealed 100% concordance between automated evaluation and the pathologist’s assessment with both the scanner and staining calibration. The proposed method may enable consistent evaluation of HER2.</p></div>","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"53 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10499734/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10260046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-obesity effects of yuja (Citrus junos Sieb ex Tanaka) pomace extract fermented with lactic acid bacteria on the hepatocytes and epididymal fat tissue of rats 乳酸菌发酵玉子渣提取物对大鼠肝细胞和附睾脂肪组织的抗肥胖作用

Applied Microscopy Pub Date : 2023-08-09 DOI: 10.1186/s42649-023-00090-9

Han-Na Chu, Haeng-Ran Kim, Kyeong-A. Jang, Yu-Jin Hwang, Jeong-Sang Kim

引用次数: 0

Fine structural characteristics of the chorionic microspheres on the egg surface of the orb web spider Trichonephila clavata 球蛛卵表面绒毛膜微球的精细结构特征

Applied Microscopy Pub Date : 2023-07-18 DOI: 10.1186/s42649-023-00087-4

Seung-Min Lee, Myung-Jin Moon

{"title":"Fine structural characteristics of the chorionic microspheres on the egg surface of the orb web spider Trichonephila clavata","authors":"Seung-Min Lee, Myung-Jin Moon","doi":"10.1186/s42649-023-00087-4","DOIUrl":"10.1186/s42649-023-00087-4","url":null,"abstract":"<div><p>The eggs laid by the orb web spider <i>Trichonephila clavata</i> must overwinter in bitterly freezing and dry conditions before hatching, but there does not seem to be any protection like a compact silk case covering the entire eggmass. Instead, the surface of the eggmass is completely coated with a milky coating called chorionic microspheres (CM). Therefore, we investigated the fine structural characteristics of CM to demonstrate their ecological importance. Although the diameter of CM in outer eggmass exhibits a significant variation, the chorionic surface is coated with a single layer of CM, characterized by a consistent diameter of approximately 2.3 µm. The surface structure of aggregated CM shows short papillary projections demonstrating segmental adhesion of mucous components. CM is insoluble in water but partially soluble in anhydrous ethanol, and its spherical structure is completely decomposed by hexafluoroisopropanol (HFIP), a strong organic solvent. Since our fine structural observations clearly show that CM is not derived from vitellogenic or choriogenetic processes, the CM adhesive coatings during ovipositional process appears to be equivalent to cocoon silk for various protective functions in silken eggcase.</p></div>","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"53 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://appmicro.springeropen.com/counter/pdf/10.1186/s42649-023-00087-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4722094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent progress in the role of grain boundaries in two-dimensional transition metal dichalcogenides studied using scanning tunneling microscopy/spectroscopy 扫描隧道显微镜/光谱学研究二维过渡金属二硫族化合物中晶界作用的最新进展

Applied Microscopy Pub Date : 2023-07-17 DOI: 10.1186/s42649-023-00088-3

Hyo Won Kim

引用次数: 0

Therapeutic treatments for diabetes mellitus-induced liver injury by regulating oxidative stress and inflammation 通过调节氧化应激和炎症治疗糖尿病性肝损伤

Applied Microscopy Pub Date : 2023-07-10 DOI: 10.1186/s42649-023-00089-2

Chun-Sik Bae, Youngchan Lee, Taeho Ahn

引用次数: 2

New recorded diatoms in Holocene sediment cores from the Gonggeom-ji Wetland in Korea 韩国贡贡记湿地全新世沉积物岩心中新记录的硅藻

Applied Microscopy Pub Date : 2023-01-20 DOI: 10.1186/s42649-023-00086-5

Daeryul Kwon, Mirye Park, Hoil Lee, Jin-Young Lee, Sang Deuk Lee

引用次数: 0

Environmental factors affecting akinete germination and resting cell awakening of two cyanobacteria 影响两种蓝藻芽胞萌发和静息细胞觉醒的环境因素

Applied Microscopy Pub Date : 2023-01-17 DOI: 10.1186/s42649-023-00085-6

Daeryul Kwon, Keonhee Kim, Hyunjin Jo, Sang Deuk Lee, Suk Min Yun, Chaehong Park

{"title":"Environmental factors affecting akinete germination and resting cell awakening of two cyanobacteria","authors":"Daeryul Kwon, Keonhee Kim, Hyunjin Jo, Sang Deuk Lee, Suk Min Yun, Chaehong Park","doi":"10.1186/s42649-023-00085-6","DOIUrl":"10.1186/s42649-023-00085-6","url":null,"abstract":"<div><p>Globally, cyanobacteria frequently cause blooms that outcompete other species in the waterbody, affecting the diversity, decreasing water exchange rates, and promoting eutrophication that leads to excessive algal growth. Here, <i>Dolichospermum circinale</i> (akinetes) and <i>Microcystic aeruginosa</i> (resting cells), were isolated from the sediment in the Uiam Dam in the North Han River and near Ugok Bridge in the Nakdong River, respectively. The morphology, germination process and rates, and growth was evaluated in different environmental conditions. <i>D. cercinalis</i> germination began on day two of culturing, with maximum cell growth observed on day ten. In contrast, <i>M. aeruginosa</i> exhibited daily increase in cell density and colony size, with notable density increase on day six. Next, different environmental conditions were assessed. Akinetes exhibited high germination rates at low light intensity (5—30 µmol/m<sup>2</sup>/s), whereas resting cells exhibited high growth rates at high light intensity (50—100 µmol/m<sup>2</sup>/s). Furthermore, both cell types exhibited optimum germination and growth in media containing N and P at 20—30° at a pH of 7—9. Our study reveals the optimum conditions for the germination and growth of cyanobacterial akinetes and resting cells isolated from river sediment, respectively, and will assist in predicting cyanobacterial blooms for appropriate management.</p></div>","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"53 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://appmicro.springeropen.com/counter/pdf/10.1186/s42649-023-00085-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4679627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Changes of lysosome by L-serine in rotenone-treated hippocampal neurons 鱼藤酮处理海马神经元中l -丝氨酸对溶酶体的影响

Applied Microscopy Pub Date : 2023-01-10 DOI: 10.1186/s42649-022-00084-z

Sumin Shin, Su-Kyeong Hwang, Ji Young Mun

引用次数: 0