Applied Microscopy最新文献

Bispecific antibodies and CLEM: an analytical approach to advanced cell imaging for therapeutic strategies 双特异性抗体和CLEM：用于治疗策略的先进细胞成像分析方法

Applied Microscopy Pub Date : 2025-01-20 DOI: 10.1186/s42649-024-00106-y

Han-ul Kim, Young Kwan Kim

{"title":"Bispecific antibodies and CLEM: an analytical approach to advanced cell imaging for therapeutic strategies","authors":"Han-ul Kim, Young Kwan Kim","doi":"10.1186/s42649-024-00106-y","DOIUrl":"10.1186/s42649-024-00106-y","url":null,"abstract":"<div><p>The development of bispecific antibodies (BsAbs) represents a significant advancement in therapeutic antibody design, enabling the simultaneous targeting of two different antigens. This dual-targeting capability enhances therapeutic efficacy, particularly in complex diseases like cancer, where tumor heterogeneity presents a significant challenge for traditional treatments. By bridging two distinct pathways, BsAbs can improve specificity and minimize off-target effects, making them invaluable in therapeutic contexts. Integrating advanced imaging techniques, particularly Correlative Light and Electron Microscopy (CLEM), offers a unique opportunity to visualize the dynamic interactions of BsAbs within cellular environments. CLEM combines the strengths of optical and electron microscopy, allowing researchers to observe real-time antibody-antigen interactions at nanoscale resolution. This synergy not only deepens our understanding of BsAbs’ mechanisms of action but also provides critical insights into their spatial distribution, binding kinetics, and functional dynamics in live cells. In this review, the integration of BsAbs and CLEM paves the way for targeted therapeutic strategies, fostering the development of more effective treatments that can adapt to the complexities of disease pathology.</p></div>","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://appmicro.springeropen.com/counter/pdf/10.1186/s42649-024-00106-y","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142994997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anatomy and histology of the olfactory organ of the javelin goby Synechogobius hasta (Gobiiformes, Gobiidae)

Applied Microscopy Pub Date : 2024-12-17 DOI: 10.1186/s42649-024-00105-z

Hyun-Tae Kim

引用次数: 0

Effects of excessive bleaching on hair: comparative analysis of external morphology and internal microstructure

Applied Microscopy Pub Date : 2024-12-17 DOI: 10.1186/s42649-024-00104-0

Dong Heui Kim, Seung Hyun Oh, Byung Soo Chang

{"title":"Effects of excessive bleaching on hair: comparative analysis of external morphology and internal microstructure","authors":"Dong Heui Kim, Seung Hyun Oh, Byung Soo Chang","doi":"10.1186/s42649-024-00104-0","DOIUrl":"10.1186/s42649-024-00104-0","url":null,"abstract":"<div><p>This study investigates the impact of excessive bleaching on the external morphology and internal microstructure of hair, compared to untreated hair. Using scanning electron microscopy (SEM) and transmission electron microscopy (TEM), we observed significant changes in both the surface and internal structures of bleached hair. SEM analysis of normal hair revealed a relatively clean surface with intact cuticle scales, while bleached hair showed brittle, torn scales with a rough appearance. In areas where the cuticle was broken, remnants of endocuticle debris were still attached, contributing to the rough surface. Complete separation of the cuticle layer resulted in numerous longitudinal fissures along the exposed cortical surface of bleached hair. TEM analysis further confirmed distinct differences; in normal hair, the cuticle layer and cortex were well-separated, and a small hole was observed within the endocuticle of the cuticle cells. Conversely, in bleached hair, the cuticle layer was separated from the cortex, with numerous pores formed by the dissolution of melanin granules scattered within the cortex, specifically between the macrofibrils. No melanin granules were detected in the cortex of bleached hair, although the macrofibril structure remained intact. The findings clearly indicate that excessive bleaching leads to the loss of the cuticle layer, exposing the cortex and significantly altering the hair’s structural integrity.</p></div>","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"54 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://appmicro.springeropen.com/counter/pdf/10.1186/s42649-024-00104-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142826233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Material analysis on semi-permanent makeup needles 半永久性化妆针的材料分析。

Applied Microscopy Pub Date : 2024-11-16 DOI: 10.1186/s42649-024-00103-1

Hyun Sook Jin, Seung Hyun Oh, Byung Soo Chang

{"title":"Material analysis on semi-permanent makeup needles","authors":"Hyun Sook Jin, Seung Hyun Oh, Byung Soo Chang","doi":"10.1186/s42649-024-00103-1","DOIUrl":"10.1186/s42649-024-00103-1","url":null,"abstract":"<div><p>The cosmetic-tattoo industry is evolving every year and the microstructures of the equipment have the great potential for semi-permanent makeup applications. Present paper explores the materials and microparticles of semi-permanent makeup tattoo needles. The surface of the five-round-shader tattoo needle used in semi-permanent makeup process was examined by scanning electron microscopy, and its elemental composition was analyzed by energy-dispersive X-ray spectroscopy. The comparison of five-round-shader needles have undergone thorough observation: original five-round-shader needle and distorted five-round-shader needle. The diameter of the sharp and rounded needle tip was measured at approximately 6.80 μm, while the deformed needle tip was approximately 16 μm thick, about 2.5 times thicker than the rounded needle tip. Many rosette-shaped lead (Pb) particles and irregular clusters adhere to the welded areas and closely adjacent needle shaft surfaces. The lead particles have a diameter ranging from 4 μm to 5 μm and exhibit a grid-like structure with a consistent thickness of plate-like shape. The distorted structure of Pb in rosette-shaped formations is shown to have originated from the grinding and polishing processes during needle manufacturing. To produce sterilized tattoo needles, high-quality tattoo needle inspection processes are necessary to remove any unhygienic substances adhering to the needle surface.</p></div>","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"54 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://appmicro.springeropen.com/counter/pdf/10.1186/s42649-024-00103-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142638215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analytical microscopy techniques using coaxial and oblique illuminations to detect thin glass particulates generated from glass vials for parenteral drug products 利用同轴和斜向照明的分析显微镜技术，检测肠外药品玻璃瓶中产生的薄玻璃微粒。

Applied Microscopy Pub Date : 2024-10-23 DOI: 10.1186/s42649-024-00101-3

Adedayo M. Sanni, Adedamola A. Opalade, Armen Shamirian, Spencer Mattson, Eric Driscoll, Michael St. Martin, Shikhar Mohan, Brooke Trimmer, Tarq Bunch, Robert Ovadia, Jungjoo Yoon, Sarina Ma, Chris Foti

{"title":"Analytical microscopy techniques using coaxial and oblique illuminations to detect thin glass particulates generated from glass vials for parenteral drug products","authors":"Adedayo M. Sanni, Adedamola A. Opalade, Armen Shamirian, Spencer Mattson, Eric Driscoll, Michael St. Martin, Shikhar Mohan, Brooke Trimmer, Tarq Bunch, Robert Ovadia, Jungjoo Yoon, Sarina Ma, Chris Foti","doi":"10.1186/s42649-024-00101-3","DOIUrl":"10.1186/s42649-024-00101-3","url":null,"abstract":"<div><p>Glass vials are the most widely used primary containers for the packaging of parenteral products due to their optical clarity, general inertness, and hermetic properties, but under certain circumstances, they can pose safety concerns. Most of these issues are related to the potential formation of glass particulates through delamination or precipitation, resulting from the chemical interaction between the drug product and the inner surface of the glass vial. Hence, it is imperative for pharmaceutical companies to conduct product-vial compatibility studies to determine the appropriate packaging/container closure system. To support this development activity, scientists need to develop analytical methods to detect subvisible glass particulates in parenteral products, along with the appropriate positive controls, to facilitate detection and identification. This paper outlines the utilization of coaxial/episcopic and oblique illumination microscopy, combined with spectroscopic techniques, to detect thin glass particulates generated from a modified procedure. It also showcases the importance of angle-dependent lighting in visualizing positive control samples containing thin glass particulates. The analytical microscopy techniques discussed in this paper can assist scientists in selecting suitable container closure systems for developing parenteral products.</p></div>","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"54 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11499564/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142492495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Microstructural, mechanical, and electrochemical analysis of carbon doped AISI carbon steels 更正：掺碳 AISI 碳钢的微观结构、机械和电化学分析。

Applied Microscopy Pub Date : 2024-10-11 DOI: 10.1186/s42649-024-00102-2

Muhammad Ishtiaq, Aqil Inam, Saurabh Tiwari, Jae Bok Seol

引用次数: 0

In situ observation of catalyst nanoparticle sintering resistance on oxide supports via gas phase transmission electron microscopy 通过气相透射电子显微镜原位观察氧化物载体上的催化剂纳米颗粒烧结阻力

Applied Microscopy Pub Date : 2024-09-17 DOI: 10.1186/s42649-024-00100-4

Wonjun Kim, Kangsik Kim, Jaejin Kim, Zonghoon Lee

{"title":"In situ observation of catalyst nanoparticle sintering resistance on oxide supports via gas phase transmission electron microscopy","authors":"Wonjun Kim, Kangsik Kim, Jaejin Kim, Zonghoon Lee","doi":"10.1186/s42649-024-00100-4","DOIUrl":"10.1186/s42649-024-00100-4","url":null,"abstract":"<div><p>Oxide-supported metal catalysts are essential components in industrial processes for catalytic conversion. However, the performance of these catalysts is often compromised in high temperature reaction environments due to sintering effects. Currently, a number of studies are underway with the objective of improving the metal support interaction (MSI) effect in order to enhance sintering resistance by surface modification of the oxide support, including the formation of inhomogeneous defects on the oxide support, the addition of a rare earth element, the use of different facets, encapsulation, and other techniques. The recent developments in in situ gas phase transmission electron microscopy (TEM) have enabled direct observation of the sintering process of NPs in real time. This capability further allows to verify the efficacy of the methods used to tailor the support surface and contributes effectively to improving sintering resistance. Here, we review a few selected studies on how in situ gas phase TEM has been used to prevent the sintering of catalyst NPs on oxide supports.</p></div>","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"54 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://appmicro.springeropen.com/counter/pdf/10.1186/s42649-024-00100-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142236084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research reviews on myosin head interactions with F-actin 肌球蛋白头部与 F-肌动蛋白相互作用的研究综述。

Applied Microscopy Pub Date : 2024-08-28 DOI: 10.1186/s42649-024-00099-8

Yoon Ho Park, Gang San Song, Hyun Suk Jung

{"title":"Research reviews on myosin head interactions with F-actin","authors":"Yoon Ho Park, Gang San Song, Hyun Suk Jung","doi":"10.1186/s42649-024-00099-8","DOIUrl":"10.1186/s42649-024-00099-8","url":null,"abstract":"<div><p>The sliding filament theory and the cross-bridge model have been fundamental in understanding muscle contraction. While the cross-bridge model explains the interaction between a single myosin head and actin filament, the native myosin molecule consists of two heads. This review explores the possibility and mechanism of two-headed binding in myosin II to the actin. Recent studies using electron tomography and resonance energy transfer have provided evidence in support of the occurrence of two-headed binding. The flexibility of the regulatory light chain (RLC) appears to play a significant role in enabling this binding mode. However, high-resolution structures of the RLCs in the two-headed bound state have not yet been reported. Resolving these structures, possibly through sub-tomogram averaging or single-particle analysis, would provide definitive proof of the conformational flexibility of RLCs and their role in facilitating two-headed binding. Further investigations are also required to address questions such as the predominance of two-headed versus single-headed binding and the influence of the state of each of the heads on the other. An understanding of the mechanism of two-headed binding is crucial for developing a comprehensive model of the cross-bridge cycle of the native myosin molecule.</p></div>","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"54 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://appmicro.springeropen.com/counter/pdf/10.1186/s42649-024-00099-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142078722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clearing techniques for deeper imaging of plants and plant–microbe interactions 清除植物和植物与微生物相互作用的深度成像技术。

Applied Microscopy Pub Date : 2024-05-31 DOI: 10.1186/s42649-024-00098-9

Ki Woo Kim

{"title":"Clearing techniques for deeper imaging of plants and plant–microbe interactions","authors":"Ki Woo Kim","doi":"10.1186/s42649-024-00098-9","DOIUrl":"10.1186/s42649-024-00098-9","url":null,"abstract":"<div><p>Plant cells are uniquely characterized by exhibiting cell walls, pigments, and phenolic compounds, which can impede microscopic observations by absorbing and scattering light. The concept of clearing was first proposed in the late nineteenth century to address this issue, aiming to render plant specimens transparent using chloral hydrate. Clearing techniques involve chemical procedures that render biological specimens transparent, enabling deep imaging without physical sectioning. Drawing inspiration from clearing techniques for animal specimens, various protocols have been adapted for plant research. These procedures include (i) hydrophobic methods (e.g., Visikol™), (ii) hydrophilic methods (Sca<i>l</i>eP and ClearSee), and (iii) hydrogel-based methods (PEA-CLARITY). Initially, clearing techniques for plants were mainly utilized for deep imaging of seeds and leaves of herbaceous plants such as <i>Arabidopsis thaliana</i> and rice. Utilizing cell wall-specific fluorescent dyes for plants and fungi, researchers have documented the post-penetration behavior of plant pathogenic fungi within hosts. State-of-the-art plant clearing techniques, coupled with microbe-specific labeling and high-throughput imaging methods, offer the potential to advance the <i>in planta</i> characterization of plant microbiomes.</p></div>","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"54 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://appmicro.springeropen.com/counter/pdf/10.1186/s42649-024-00098-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141178392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Noise reduction of electron holography observations for a thin-foiled Nd-Fe-B specimen using the wavelet hidden Markov model 利用小波隐马尔可夫模型降低薄箔钕铁硼试样电子全息观测数据的噪声

Applied Microscopy Pub Date : 2024-04-17 DOI: 10.1186/s42649-024-00097-w

Sujin Lee, Yoshihiro Midoh, Yuto Tomita, Takehiro Tamaoka, Mitsunari Auchi, Taisuke Sasaki, Yasukazu Murakami

引用次数: 0