Journal of Taibah University Medical Sciences最新文献

{"title":"Comparison of progress test performance between PBL and TBL curricula","authors":"Ahmad S. Alamro PhD","doi":"10.1016/j.jtumed.2026.03.008","DOIUrl":"10.1016/j.jtumed.2026.03.008","url":null,"abstract":"<div><h3>Background</h3><div>Problem-based learning (PBL) and team-based learning (TBL) are two widely implemented educational strategies in medical education. Although both are aimed at enhancing student engagement and clinical reasoning, limited research has compared their long-term outcomes by using curriculum-independent measures such as progress testing.</div></div><div><h3>Methods</h3><div>This retrospective comparative study examined progress test performance among medical students enrolled in PBL vs. TBL tracks at the same university. From de-identified data, student scores were analyzed across five academic years, stratified by gender, discipline, body system, dimension of care, and physician activity.</div></div><div><h3>Results</h3><div>TBL students scored higher in early years, whereas PBL students showed a steeper knowledge trajectory and outperformed their TBL peers in senior years. The PBL students consistently achieved higher scores across most disciplines, organ systems, and care dimensions, particularly in chronic care, preventive care, diagnosis, and psychosocial domains. Analysis by gender indicated slightly higher performance among women than men in both tracks.</div></div><div><h3>Conclusion</h3><div>Curricular structure critically shapes students’ patterns of knowledge acquisition. The findings suggested that PBL and TBL support learning in different ways across the curriculum, with each demonstrating strengths at different stages of student progression. These results highlight the potential value of designing blended curricula drawing on the complementary features of both approaches to optimize medical education outcomes.</div></div>","PeriodicalId":46806,"journal":{"name":"Journal of Taibah University Medical Sciences","volume":"21 2","pages":"Pages 367-375"},"PeriodicalIF":1.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147656191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lateral intubation with the Macintosh laryngoscope and C-MAC D blade videolaryngoscope (LATIN): A randomized controlled trial","authors":"Renjith Viswanath MD ,&nbsp;Sryma PB DM ,&nbsp;Ganesan C MD ,&nbsp;Ganga Reghupathy MBBS","doi":"10.1016/j.jtumed.2026.03.010","DOIUrl":"10.1016/j.jtumed.2026.03.010","url":null,"abstract":"<div><h3>Background and aims</h3><div>Lateral intubation is frequently encountered outside operating room settings, particularly for endoscopic procedures. Challenges faced by anesthesiologists include a lack of training and experience in performing intubation in the lateral position, and the paucity of data regarding the ideal method. This study was aimed at comparing the glottis visualization times and intubation times with Macintosh laryngoscopes and C-MAC D blade video laryngoscopes in American Society of Anesthesiologists (ASA) 1 and 2 patients without any risk factors for difficult airway, in the right lateral decubitus position.</div></div><div><h3>Materials and methods</h3><div>The primary outcome was glottis visualization time. Secondary outcomes included intubation time, bougie use, intubation difficulty score, use of external laryngeal pressure, number of operators required, and complications. All intubators were trained experienced operators trained in lateral intubation on mannequins. Clinical trial registration is available at <span><span>https://ctri.nic.in/Clinicaltrials/login.php</span><svg><path></path></svg></span>, ID: CTRI/2024/06/068243.</div></div><div><h3>Results</h3><div>This randomized controlled trial included 62 patients, 31 per group, undergoing elective procedures under general anesthesia and requiring endotracheal intubation. The patients were able to be intubated in the lateral position with a success rate of 96.8%. The mean glottis visualization time was 7.9 ± 3.1 s in the Macintosh (M) group (n = 32) and 6.8 ± 3.1 s in the C-MAC D blade (C) group (n = 29), and did not significantly differ between groups (p = 0.14). The intubation time was 7.5 ± 5 s (n = 31) in group M and 6 ± 3.5 s (n = 29) in group C, and did not significantly differ between groups (p = 0.61). No major complications were observed.</div></div><div><h3>Conclusion</h3><div>Both Macintosh and C-MAC D blade laryngoscopes can be effectively used by trained clinicians for intubation in the lateral position.</div></div><div><h3>Registration details</h3><div>Clinical trial registration is available at <span><span>https://ctri.nic.in/Clinicaltrials/login.php</span><svg><path></path></svg></span>, ID: CTRI/2024/06/068243.</div></div>","PeriodicalId":46806,"journal":{"name":"Journal of Taibah University Medical Sciences","volume":"21 2","pages":"Pages 361-366"},"PeriodicalIF":1.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147656192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of functional capacity and clinical outcomes in healthy female college students: A cross-sectional study","authors":"Tarek M. El-gohary PhD","doi":"10.1016/j.jtumed.2026.03.011","DOIUrl":"10.1016/j.jtumed.2026.03.011","url":null,"abstract":"<div><h3>Objectives</h3><div>This study was aimed at comparing step-up-and-down (SUD) test performance between the dominant and non-dominant lower limbs, and examining the association between isokinetic strength measures and functional performance in college students.</div></div><div><h3>Methods</h3><div>A cross-sectional study was conducted in 39 healthy females. Participants performed the SUD test on both lower limbs. The isokinetic strength of the knee extensors was assessed at angular velocities of 60°/s and 180°/s with a calibrated isokinetic dynamometer.</div></div><div><h3>Results</h3><div>No significant differences were observed in SUD test performance between the dominant and non-dominant limbs (<em>p</em> &gt; 0.05). Significant moderate negative correlations were found between SUD performance and isokinetic strength measures. SUD time with the dominant limb correlated with peak torque (ρ = −0.40, <em>p</em> = 0.02), average torque (ρ = −0.36, <em>p</em> = 0.04), and average power (ρ = −0.61, <em>p</em> &lt; 0.001), whereas SUD time with the non-dominant limb correlated with average power (ρ = −0.41, <em>p</em> = 0.02). Therefore, greater muscular strength was associated with faster functional performance.</div></div><div><h3>Conclusions</h3><div>Although no significant functional differences were observed, integrating isokinetic strength testing with functional assessments enables more comprehensive evaluation of lower-limb performance, enhances the detection of subtle strength–function discrepancies, and supports targeted, individualized rehabilitation and preventive strategies.</div></div>","PeriodicalId":46806,"journal":{"name":"Journal of Taibah University Medical Sciences","volume":"21 2","pages":"Pages 376-383"},"PeriodicalIF":1.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147656190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of a pulmonary rehabilitation program in a patient with very severe chronic obstructive pulmonary disease at a primary care center: A case report","authors":"Mohammed E. Alsubaiei PhD","doi":"10.1016/j.jtumed.2025.12.009","DOIUrl":"10.1016/j.jtumed.2025.12.009","url":null,"abstract":"","PeriodicalId":46806,"journal":{"name":"Journal of Taibah University Medical Sciences","volume":"21 1","pages":"Pages 129-134"},"PeriodicalIF":1.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146023188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In silico evaluation of Alstonia scholaris phytochemicals against periodontal pathogens","authors":"Muhammad I. Rizal PhD ,&nbsp;Syafia Husain BDS ,&nbsp;Komariah Komariah PhD ,&nbsp;Ciptadhi T. Binartha PhD ,&nbsp;Arief Cahyanto PhD ,&nbsp;Moehamad O. Roeslan PhD","doi":"10.1016/j.jtumed.2025.12.006","DOIUrl":"10.1016/j.jtumed.2025.12.006","url":null,"abstract":"<div><h3>Objectives</h3><div><em>Porphyromonas gingivalis</em> and <em>Treponema denticola</em> are red complex pathogens strongly associated with periodontal disease. <em>P. gingivalis</em> relies on the heme-binding receptor HmuY for heme acquisition, whereas <em>T. denticola</em> uses factor H-binding protein (FhbB) to evade immune responses. This study was aimed at evaluating the inhibitory potential of three phytocompounds (alstonine, echitamine chloride, and villalstonine) from <em>Alstonia scholaris</em> against these targets, which were compared with minocycline and chlorhexidine, via molecular docking.</div></div><div><h3>Methods</h3><div>Molecular docking was performed with validation by redocking and confirmation of receptor reliability (RMSD 1.821 Å for HmuY and 0.851 Å for FhbB). Chlorhexidine and minocycline were included as positive controls. Binding energies (ΔG), inhibition constants (Ki), and receptor–ligand interactions were analyzed.</div></div><div><h3>Results</h3><div>Echitamine chloride exhibited the strongest affinity for HmuY (ΔG −4.86 kcal/mol; Ki 274.96 μM), whereas alstonine bound most strongly to FhbB (ΔG −7.44 kcal/mol; Ki 3.53 μM). Villalstonine showed moderate affinity, whereas minocycline was the most potent overall. Key hydrogen bonds involved ARG178, TRP70, and TYR116.</div></div><div><h3>Conclusions</h3><div><em>A. scholaris</em> phytochemicals, particularly echitamine chloride and alstonine, displayed promising but weaker inhibitory potential than standard antimicrobials. They therefore might serve as natural adjuncts in periodontal therapy, pending experimental validation.</div></div>","PeriodicalId":46806,"journal":{"name":"Journal of Taibah University Medical Sciences","volume":"21 1","pages":"Pages 25-30"},"PeriodicalIF":1.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145885891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploratory analysis of immune profiles in 2- to 3-year-old children with growth stunting","authors":"Rizana Fajrunni'mah M.Si.Med. ,&nbsp;Mohamad Sadikin D.Sc. ,&nbsp;Heri Wibowo Ph.D. ,&nbsp;Dwirini Retno Gunarti Ph.D.","doi":"10.1016/j.jtumed.2026.01.004","DOIUrl":"10.1016/j.jtumed.2026.01.004","url":null,"abstract":"<div><h3>Background</h3><div>Growth stunting, which is associated with metabolic and immunological changes, remains a concerning global challenge. However, the etiology and underlying pathophysiological mechanisms remain elusive.</div></div><div><h3>Objectives</h3><div>This study was aimed at exploring humoral, cytokine, and T-cell immune profiles in children 2–3 years of age, with or without stunting.</div></div><div><h3>Methods</h3><div>A cross-sectional exploratory study was conducted among children with or without stunting. Antibody isotypes (IgM, IgG1, IgG2, IgG3, IgG4, IgA, and IgE) were measured with a ProcartaPlex Human Antibody Isotyping Panel 7-Plex kit. Proportions of T lymphocytes and Tregs were determined through flow cytometry. Cytokine levels (IL-6, TNF-α, and IL-10) were measured with a human cytokine kit, read with an x-MAP (Luminex200) instrument. Group differences were analyzed with t-tests or Mann–Whitney tests. False discovery rate (FDR) correction was applied. Multivariable logistic regression provided adjusted odds ratios (AORs). ROC-derived cut-off points were used for exploratory quadrant analysis of significant biomarkers.</div></div><div><h3>Results</h3><div>Unadjusted analyses indicated higher IgM, IgG2, IgG3, IL-6, and IL-6/IL-10 ratios in children with rather than without stunting (p ≤ 0.05). After FDR correction, no biomarkers achieved conventional significance. However, several markers, including IL-6 and IgG3, approached the exploratory threshold (FDR &lt; 0.20) commonly accepted in hypothesis-generating immunological analyses. In multivariable models, IL-6 remained associated with stunting (AOR 1.731; 95% CI 1.044–2.870), and IgG3 showed the largest effect estimate (AOR 4.055; 95% CI 0.813–20.226), despite non-significance. Quadrant analysis demonstrated substantially elevated odds of stunting in children with concurrent high IL-6 and high IgG3 (OR 9.56–18.7).</div></div><div><h3>Conclusion</h3><div>Exploratory analyses indicate biologically plausible inflammatory-humoral activation signatures in children with stunting, warranting confirmation in larger longitudinal studies.</div></div>","PeriodicalId":46806,"journal":{"name":"Journal of Taibah University Medical Sciences","volume":"21 1","pages":"Pages 139-148"},"PeriodicalIF":1.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146078730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pain determinants during office hysteroscopy: Insights from clinical and procedural factors: A nine-year prospective cohort study","authors":"Wassan Nori MD,PhD ,&nbsp;Zeena R. Helmi MD,PhD ,&nbsp;Rafal M. Murshid MD,PhD","doi":"10.1016/j.jtumed.2025.12.011","DOIUrl":"10.1016/j.jtumed.2025.12.011","url":null,"abstract":"","PeriodicalId":46806,"journal":{"name":"Journal of Taibah University Medical Sciences","volume":"21 1","pages":"Pages 104-113"},"PeriodicalIF":1.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146023121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thermoresponsive propolis gel for gingival wound healing post-depigmentation: A prospective clinical study","authors":"Naina Pattnaik MDS (Periodontics), Associate Professor ,&nbsp;Smitapadma Mohanty PhD (Pharmacy) ,&nbsp;Dhirendra K. Singh MDS (Periodontics), Professor ,&nbsp;Anasuya Sahoo M.Pharm, Master (Pharmacy), Associate Professor ,&nbsp;Jugajyoti Pathi MDS (Oral and Maxillofacial Surgery), Associate Professor ,&nbsp;Md Jalaluddin MDS (Periodontics), Professor","doi":"10.1016/j.jtumed.2025.12.002","DOIUrl":"10.1016/j.jtumed.2025.12.002","url":null,"abstract":"<div><h3>أهداف البحث</h3><div>على الرغم من أن فرط تصبغ اللثة حميد، إلا أنه غالبا ما يثير مخاوف جمالية قد تؤثر على تقدير الذات. وقد أصبحت تقنية إزالة التصبغ بمساعدة الليزر تقنية مفضلة، نظرا لدقتها وقلة تدخلها الجراحي. ومع ذلك، لا يزال تحسين الشفاء بعد الجراحة أولوية سريرية. قيّمت هذه الدراسة فعالية جل موضعي جديد مُستجيب للحرارة، قائم على البروبوليس، في تعزيز التئام الأنسجة الرخوة بعد إجراءات إزالة التصبغ بالليزر.</div></div><div><h3>طرق البحث</h3><div>أجريت تجربة سريرية مستقبلية مضبوطة بدواء وهمي على 66 فردا سليما تتراوح أعمارهم بين 15 و30 عاما، خضعوا لإزالة تصبغ اللثة بالليزر الثنائي، مقتصرة على اللثة الشفوية الأمامية العلوية (من الناب إلى الناب). خضعت جميع الإجراءات لمعايير موحدة، وأُجريت من قِبل طبيب معاير واحد. وُزّع المشاركون عشوائيا على مجموعتين: مجموعة اختبار تلقت جل موضعي قائم على البروبوليس، ومجموعة ضابطة تلقت جلا وهميا خاليا من المكونات النشطة. أظهر الجل خصائص إطلاق متواصل، ولصق مخاطي، واستجابة حرارية، وتم تقييم ثباته الفيزيائي ونمط إطلاقه في المختبر. تم تقييم الشفاء سريريا باستخدام مؤشر لاندري للشفاء في نهاية الأسابيع الأول والثاني والثالث بعد الجراحة. تم تسجيل الانزعاج بعد الجراحة باستخدام مقياس التناظر البصري. تم تحليل البيانات إحصائيا.</div></div><div><h3>النتائج</h3><div>أظهرت مجموعة الاختبار تحسنا ملحوظا في درجات الشفاء في جميع نقاط التقييم مقارنة بالمجموعة الضابطة. أشارت درجات مقياس التناظر البصري إلى انزعاج متقارب بعد الجراحة بين المجموعتين، مما يشير إلى تحمّل إيجابي للعلاج. حافظت التركيبة على ثباتها، ولصقها المخاطي، واستمرار إطلاق الدواء لأكثر من 12 ساعة.</div></div><div><h3>الاستنتاجات</h3><div>يُعد جلنا الموضعي المستجيب للحرارة، القائم على البروبوليس، علاجا آمنا وفعالا لتعزيز التئام الأنسجة الرخوة بعد إزالة تصبغ اللثة بالليزر. تدعم سهولة استخدامه وفوائده السريرية تطبيقه على نطاق أوسع في إجراءات اللثة والأنسجة الرخوة الفموية.</div></div>","PeriodicalId":46806,"journal":{"name":"Journal of Taibah University Medical Sciences","volume":"21 1","pages":"Pages 67-77"},"PeriodicalIF":1.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145978085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From diagnostics to education: Multi-domain evaluation of LLM chatbots in neurology","authors":"Gopi Battineni PhD, MS, ME ,&nbsp;Nalini Chintalapudi PhD, MTech ,&nbsp;Venkata R. Dhulipalla PhD, MTech ,&nbsp;Francesco Amenta PhD, MD","doi":"10.1016/j.jtumed.2025.11.004","DOIUrl":"10.1016/j.jtumed.2025.11.004","url":null,"abstract":"<div><h3>Objectives</h3><div>The development of large language models (LLMs) has shown promising results in enhancing research processes, data analysis, and communication in various domains of neurology. In this work, we systematically review and synthesize current evidence on the applications of LLMs in the assessment, diagnosis, and monitoring of neurological disorders.</div></div><div><h3>Methods</h3><div>Three databases, namely PubMed, Scopus, and Web of Science, were considered for document search. Article selection was according to PRISMA guidelines, and Newcastle–Ottawa Scale (NOS) was used to assess the article quality based on relevance, quality, and applicability.</div></div><div><h3>Results</h3><div>Nine studies were included in the final analysis. Based on the findings, LLMs have been utilized in diverse areas of neuroscience including hypothesis generation, clinical decision support, and cognitive modeling. LLMs can process large datasets, identify trends, and support personalized medicine. However, challenges such as interpretability, ethical considerations, and domain-specific training remain critical.</div></div><div><h3>Conclusions</h3><div>By facilitating workflows and uncovering new insights, LLMs can revolutionize different domains of neurology. Nevertheless, further research on their reliability, ethical implications, and adaptation to the unique demands of neuroscience is needed.</div></div>","PeriodicalId":46806,"journal":{"name":"Journal of Taibah University Medical Sciences","volume":"21 1","pages":"Pages 15-24"},"PeriodicalIF":1.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145842385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mesenchymal and neural stem cell differentiation into Purkinje-like cells: Morphological study","authors":"Maeda H. Mohammad PhD ,&nbsp;Ahmed M. Al-Shammari PhD ,&nbsp;Ahmad A. Aljuboori PhD ,&nbsp;Rafal Hussamildeen PhD ,&nbsp;Zaid A. Al-Hilli PhD","doi":"10.1016/j.jtumed.2026.01.009","DOIUrl":"10.1016/j.jtumed.2026.01.009","url":null,"abstract":"<div><h3>أهداف البحث</h3><div>تُعدّ خلايا بيركنجي فئةً محورية من الخلايا العصبية المُثبِّطة في الجهاز العصبي المركزي، وقد ارتبطخلل وظيفتها بطيفٍ من الاضطرابات العصبية. وقد أتاح عزل خلايا بيركنجي وتكثيرها تحقيق تقدّمٍ في مجال علم الأعصاب التجديدي، بما يدعم تطوير العلاجات المعتمدة على الخلايا لاستعادة التوازن التثبيطي في الدوائر العصبية المصابة. هدفت هذه الدراسة إلى تطوير طريقةٍ سهلة لتوليد خلايا شبيهة بخلايا بيركنجي داخل المختبر من الخلايا الجذعية العصبية المستمدة من الخلايا الجذعية الميزنكيمية لنخاع العظم، وكذلك من الخلايا الجذعية العصبية المستمدة من الفص الشمي، تمهيدًا لاستخدامها في تطبيقات سريرية مستقبلية.</div></div><div><h3>طرق البحث</h3><div>تم عزل الخلايا الجذعية الميزنكيمية من نخاع عظم الفئران وتحفيز تمايزها إلى خلايا جذعية عصبية. كماجرى عزل خلايا جذعية عصبية مباشرةً من الفص الشمي للفئران. وتم عزل خلايا بيركنجي باستخدام خرزاتٍ مغناطيسية. واستُخدمت خلايا بيركنجي المعزولة لتحفيز تمايز الخلايا الجذعية العصبية إلى خلايا شبيهة بخلايا بيركنجي عبر طريقتي المُحلَّل الخلوي والمشاركة في الزراعة. وجرت دراسة خصائص الخلايا الناتجة وأنماطها الظاهرية اعتمادًا على عدد التفرعات وطولها، وطول المحور العصبي، إضافةً إلى التحليل المناعي الخلوي.</div></div><div><h3>النتائج</h3><div>تم بنجاح عزل وتكثير الخلايا الجذعية الميزنكيمية، والخلايا الجذعية العصبية، والخلايا الشبيهة بخلايا بيركنجيفي الزراعة الخلوية، كما أظهرت التحاليل المناعية الخلوية إيجابيةً للواسمات الظاهرية ذات الصلة، مثل واسمات الخلايا الجذعية الميزنكيمية، وواسم الخلايا الجذعية العصبية، وواسم خلايا بوركنجي. كما أظهرت خلايا بيركنجي المُنتَجة أطوالًا أكبر للمحاور العصبية، وعددًا وطولًا أكبر للتفرعات مقارنةً بأنواع الخلايا الأخرى. إضافةً إلى ذلك، نجحت كلٌّ من طريقتي المُحلَّل الخلوي والمشاركة في الزراعة في تكثير الخلايا الشبيهة بخلايا بيركنجي داخل المختبر.</div></div><div><h3>الاستنتاجات</h3><div>أثبتت الطرائق المعتمدة على المشاركة في الزراعة لخلايابيركنجي المعزولة والمنقّاة و/أو استخدام مُحلَّلاتهاالخلوية فعاليتها في تمايز الخلايا الجذعية العصبية إلى خلايا تحمل صفاتٍ ظاهرية شبيهة بخلايا بيركنجي.</div></div>","PeriodicalId":46806,"journal":{"name":"Journal of Taibah University Medical Sciences","volume":"21 1","pages":"Pages 164-177"},"PeriodicalIF":1.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146173255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}