评估谷胱甘肽过氧化物酶1和4在口腔鳞状细胞癌患者在三级保健中心的表达：一项比较横断面研究

IF 1.5 Q2 MEDICINE, GENERAL & INTERNAL

Journal of Taibah University Medical Sciences Pub Date : 2025-06-01 DOI:10.1016/j.jtumed.2025.06.003

Sadia Farooqi MDS , Shazia Akbar Ansari PhD , Farheen Danish M.Phil , Rida Fatima Khan MDS , Ammar Ali Muhammad MSc , Aliya Zaman M.Phil

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引用次数: 0

摘要

谷胱甘肽过氧化物酶1 （gtathione peroxidase 1, GPX1）和GPX4是哺乳动物组织细胞质中丰富的抗氧化酶。这些酶具有清除自由基的能力，在多种癌症，特别是口腔鳞状细胞癌（OSCC）中发挥重要作用。比较GPX1和GPX4在OSCC原发肿瘤不同病理分期中的表达水平。2. 评估GPX1和GPX4表达与其他自变量（年龄、性别、病变部位、侧边性、组织病理分级、OSCC肿瘤淋巴结累及）的关系。方法从陶氏大学陶氏诊断研究与转诊实验室选取133例前瞻性OSCC组织标本。免疫组织化学检测GPX1和GPX4的表达水平。使用ImageJ软件获取免疫反应评分（IRS）值。根据肿瘤病理分期及其他自变量比较GPX1和GPX4的IRS值。数据采用SPSS 21和STATA进行统计分析。结果在133份样本中，GPX4存在于所有阶段的口腔癌中，高IRS值大多对应于中至强染色强度。相比之下，GPX1在口腔癌的所有分期中均未发现，低IRS值多与弱染色强度相对应。没有发现与其他变量的关系。结论在口腔癌的所有分期和所有组织样本中均存在ongpx4，而GPX1未在所有病例中发现。GPX1和GPX4的IRS值与口腔癌的病理分期有显著关系。

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Assessment of glutathione peroxidase 1 and 4 expression in oral squamous cell carcinoma patients in a tertiary care center: A comparative cross-sectional study

Background

Glutathione peroxidase 1 (GPX1) and GPX4 are abundant antioxidant enzymes within the cytosol in mammalian tissues. These enzymes have the capacity to scavenge free radicals, and they play roles in various cancers, especially oral squamous cell carcinoma (OSCC).

Objectives

1. To compare the expression levels of GPX1 and GPX4 among different pathological stages for OSCC primary tumors. 2. To assess the associations of GPX1 and GPX4 expression with other independent variables (age, gender, site of lesion, laterality, histopathological grades, and lymph node involvement of OSCC tumor).

Methods

In total, 133 prospective OSCC tissue specimens were selected from Dow Diagnostic Research and Referral Laboratory of Dow University. Expression levels of GPX1 and GPX4 were evaluated by immunohistochemistry. Immunoreactive score (IRS) values were obtained using ImageJ software. The IRS values for GPX1 and GPX4 were compared according to the pathological stages of cancer and other independent variables. Data were statistically analyzed by using SPSS version 21 and STATA.

Results

Among the 133 samples, GPX4 was present in all stages of oral cancer and high IRS values mostly corresponded to moderate to strong staining intensity. By contrast, GPX1 was not found in all stages of oral cancer, and low IRS values mostly corresponded to weak staining intensity. No relationships were found with other variables.

Conclusion

GPX4 was present in all stages of oral cancer and in all tissue samples, whereas GPX1 was not found in all cases. The IRS values for GPX1 and GPX4 had significant relationships with pathological stages of oral cancer.

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