Applied Biological Chemistry最新文献

{"title":"In vitro antibacterial, antioxidant, in silico molecular docking and ADEMT analysis of chemical constituents from the roots of Acokanthera schimperi and Rhus glutinosa","authors":"Bihon Abera,&nbsp;Yadessa Melaku,&nbsp;Kebede Shenkute,&nbsp;Aman Dekebo,&nbsp;Negera Abdissa,&nbsp;Milkyas Endale,&nbsp;Temesgen Negassa,&nbsp;Messay Woldemariam,&nbsp;Mo Hunsen","doi":"10.1186/s13765-024-00930-6","DOIUrl":"10.1186/s13765-024-00930-6","url":null,"abstract":"<div><p><i>Acokanthera schimperi</i> is a medicinal plant traditionally used for the treatment of wounds, scabies, and malaria. <i>Rhus glutinosa</i> has been also utilized for the management of ectoparasites and hemorrhoids. Silica gel column chromatography separation of CH₂Cl₂/MeOH (1:1) extract root of <i>A. schimperi</i> afforded oleic acid (<b>1</b>), lupeol (<b>2</b>), dihydroferulic acid (<b>3</b>), acovenosigenin A- 3-O-α-L-rhamnopyranoside (<b>4</b>) and sucrose (<b>5</b>) whereas CH₂Cl₂/ MeOH (1:1) and MeOH roots extracts of <i>R. glutinosa</i> afforded β-sitosterol (<b>6</b>), (E)-5-(heptadec-14-en-1-yl)-4,5-dihydroxycyclohex-2-enone (<b>7</b>), methyl gallate (<b>8</b>), and gallic acid (<b>9</b>). The structures of the compounds were established using spectroscopic (1D and 2D NMR) and FT-IR techniques. Disc diffusin and DPPH assay were used, respectively, to evaluate the antibacterial and antioxidant potential of the extracts and isolated compounds. MeOH extract root of <i>A. schimperi</i> showed a modest antibacterial effect against <i>E.coli</i> with an inhibition zone (ZI) of 16 ± 0.0 mm compared to ciprofloxacin (ZI of 27.0 ± 0.0 mm). CH₂Cl₂/MeOH (1:1) and MeOH root extracts of <i>R. glutinosa</i> showed maximum activity against <i>S. aureus</i> with ZI of 17.3 ± 0.04 and 18.0 ± 0.0 mm, respectively. At 5 mg/mL, the highest activity was noted against <i>S. aureus</i> by <b>8</b> with ZI of 18.6 ± 0.08 mm. Dihydroferulic acid (<b>3</b>), methyl gallate (<b>8</b>), and gallic acid (<b>9</b>) displayed potent scavenging of DPPH radical with respective IC₅₀ of 10.66, 7.48, and 6.08 µg/mL, compared with ascorbic acid (IC₅₀ of 5.83 µg/mL). Molecular docking results showed that lupeol (<b>2</b>) exhibited strong binding energy of -7.7 and − 10 kcal/mol towards PDB ID: 4F86 and PDB ID: 3T07, respectively, compared to ciprofloxacin (-6.5 and − 7.2 kcal/mole). Towards PDB ID: 1DNU receptor, compounds <b>3</b>, <b>8</b>, and <b>9</b> showed minimum binding energy of -5.1, -4.8, and − 4.9 kcal/mol, respectively, compared to ascorbic acid (-5.7 kcal/mol). The Swiss ADME prediction results indicated that compounds <b>2</b>, <b>3</b>, <b>8</b>, and <b>9</b> obeyed the Lipinksi rule of five and Veber rule with 0 violations. The in vitro antibacterial and antioxidant results supported by <i>in silico</i> analysis indicated that compounds <b>2</b>, <b>3</b>, <b>8</b>, and <b>9</b> can potentially be lead candidates for the treatment of pathogenic and free radical-induced disorders.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00930-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142123077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential endocrine-disrupting effects of iprodione via estrogen and androgen receptors: evaluation using in vitro assay and an in silico model","authors":"Ji-Yeon Yang,&nbsp;Jeong-Hyun Lim,&nbsp;Soo-Jin Park,&nbsp;Youmi Jo,&nbsp;Si Young Yang,&nbsp;Min-Kyoung Paik,&nbsp;So-Hye Hong","doi":"10.1186/s13765-024-00932-4","DOIUrl":"10.1186/s13765-024-00932-4","url":null,"abstract":"<div><p>This study was conducted to provide evidence, using in vitro and in silico testing methods, regarding the adverse effects of iprodione, a representative dichlorophenyl dicarboxamide fungicide, on the endocrine system. In the present study, we used the HeLa9903 stably transfected transactivation assay (OECD TG 455), 22Rv1/MMTV_GR‒KO androgen receptor transcriptional activation assay (OECD TG 458), and toxicity prediction using VEGA QSAR. Our results showed that iprodione had no estrogen receptor antagonistic or androgen receptor agonistic effects; however, iprodione was determined to be an estrogen receptor agonist (log PC₁₀ value is less than − 9) and androgen receptor antagonist (log IC₃₀ value is − 4.58) without intrinsic toxicity against the human cell lines used in this study. VEGA QSAR was used to evaluate five substances with structures similar to that of iprodione. Among them, four chemicals were found to have positive androgen receptor and aromatase activities and have been observed to be developmental toxicants. These results suggest that iprodione regulates steroid hormone receptor interactions and is a potential reproductive toxicant.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00932-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142091161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The green approach of chitosan/Fe2O3/ZnO-nanocomposite synthesis with an evaluation of its biological activities","authors":"Aisha M. H. Al-Rajhi,&nbsp;Tarek M. Abdelghany,&nbsp;Mohammed S. Almuhayawi,&nbsp;Mohammed H. Alruhaili,&nbsp;Soad K. Al Jaouni,&nbsp;Samy Selim","doi":"10.1186/s13765-024-00926-2","DOIUrl":"10.1186/s13765-024-00926-2","url":null,"abstract":"<div><p>Biopolymers embedded with nanoparticles of metal oxides (MOs) demonstrate a wide range of bio-functions. Chitosan-incorporated MOs are an interesting class of support matrices for enhancing the biological function, compared to other support matrices. Therefore, the importance of this study lies in exploiting chitosan as a carrier not of one metal as in previous studies, but of two metals in the form of a nanocomposite to carry out several biological functions. The coprecipitation approach was employed to synthesize chitosan/Fe₂O₃/ZnO-nanocomposite in the present research. The characterization of chitosan/Fe₂O₃/ZnO-nanocomposite was performed to find out the morphology and dispersion properties of chitosan/Fe₂O₃/ZnO-nanocomposite. The X-ray diffraction (XRD) investigation revealed that these were crystalline. Fourier transforms infrared (FTIR) spectrum bands were viewed at 400/cm and 900/cm, due to the stretching vibration of Fe and Zn oxygen bond. TEM showed that chitosan/Fe₂O₃/ZnO-nanocomposite was of 20–95 nm in size. chitosan/Fe₂O₃/ZnO-nanocomposite exhibited inhibitory potential against <i>Staphylococcus aureus</i>,<i> Bacillus subtilis, Escherichia coli</i>, and <i>Candida albicans</i> with inhibition zones of 25 ± 0.1, 28 ± 0.2, 27 ± 0.1, and 27 ± 0.2 mm, respectively while didn’t inhibited <i>Aspergillus niger</i>. MIC value of nanocomposite was 15.62 ± 0.33 µg/mL for<i> C. albicans, B. subtilis</i> and <i>E. coli</i>, while it was 62.50 ± 0.66 µg/mL for <i>Pseudomonas aeruginosa</i>. Ranged values of nanocomposite MBC (15.62 ± 0.33 to 125 ± 1 µg/mL) were attributed to all tested bacteria. Different concentrations of chitosan/Fe₂O₃/ZnO-nanocomposite MBC (25, 50, and 75%) reflected anti-biofilm activity against<i> E. coli</i> (85.0, 93.2, and 96.0%),<i> B. subtilis</i> (84.88, 92.21, and 96.99%), <i>S. aureus</i> 81.64, 90.52, and 94.64%) and <i>P. aurogenosa</i> (90.11, 94.43, and 98.24%), respectively. The differences in the levels of antimicrobial activities may depend on the type of examined microbes. Antioxidant activity of chitosan/Fe₂O₃/ZnO-nanocomposite was recorded with excellent IC₅₀ values of 16.06 and 32.6 µg/mL using DPPH and ABTS scavenging, respectively. Wound heal by chitosan/Fe₂O₃/ZnO-nanocomposite was achieved with 100% compared to the untreated cells (76.75% of wound closer). The cytotoxicity outcomes showed that the IC₅₀ of the chitosan/Fe₂O₃/ZnO-nanocomposite was 564.32 ± 1.46 µg/mL normal WI-38 cells. Based on the achieved findings, the chitosan/Fe₂O₃/ZnO-nanocomposite is a very promising agent for perform pharmacological activities.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00926-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142091153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing seed oil content and fatty acid composition in camelina through overexpression of castor RcWRI1A and RcMYB306","authors":"Mid-Eum Park,&nbsp;Inyoung Kim,&nbsp;Hye Ji Lee,&nbsp;Mi Chung Suh,&nbsp;Kyeong-Ryeol Lee,&nbsp;Hyun Uk Kim","doi":"10.1186/s13765-024-00927-1","DOIUrl":"10.1186/s13765-024-00927-1","url":null,"abstract":"<div><p>Seed triacylglycerol (TAG), a major component of vegetable oil, consists of a glycerol esterified with three fatty acids. Vegetable oil has industrial applications and is widely used as edible oil. The increasing demand for plant oils, owing to population growth, it is crucial to enhance the oil content in seeds. We found castor WRINKLED1A (RcWRI1A) and R2R3-type MYB domain protein 306 (RcMYB306) which have homology with Arabidopsis WRI1 (AtWRI1) and AtMYB96 which regulate genes involved in fatty acid and TAG synthesis, respectively. These castor genes were separately and jointly overexpressed using seed-specific promoters in an oil crop, camelina (<i>Camelina sativa</i>). Overexpression of <i>RcWRI1A</i>, <i>RcMYB306</i>, or <i>RcWRI1A</i> + <i>RcMYB306</i> increased the total seed oil content in camelina. However, this increase was not significantly different from that observed during the overexpression of <i>RcWRI1A</i> or/and <i>RcMYB306</i>. <i>RcWRI1A</i> overexpression increased the fatty acid content, including 16:0, 18:2, 18:3. Contrastingly, <i>RcMYB306</i> overexpression increased the 18:1, 18:2, 18:3, 20:0 and 20:1 fatty acid. In the <i>RcWRI1A + RcMYB306</i> lines, changes in fatty acid composition demonstrated the combined effects of these transcription factors. These results suggest that RcWRI1A and RcMYB306 can be used to improve the productivity of oil crops.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00927-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142050493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of halogens on 3-[4-(dimethylamino) phenyl]-1-phenylprop-2-en-1-ones: development of a new class of monoamine oxidase-B inhibitors","authors":"Haydara Ammar Hasan,&nbsp;Jiseong Lee,&nbsp;Sunil Kumar,&nbsp;Saleh Alfarraj,&nbsp;Sulaiman Ali Alharbi,&nbsp;Manu Pant,&nbsp;Hoon Kim,&nbsp;Bijo Mathew","doi":"10.1186/s13765-024-00929-z","DOIUrl":"10.1186/s13765-024-00929-z","url":null,"abstract":"<div><p>Five dimethylamino-based chalcone derivatives (AC) were synthesized and evaluated for their inhibition degree against monoamine oxidase (MAO) enzymes. All AC compounds showed better inhibitory activity against MAO-B than that against MAO-A. AC4 showed the highest inhibitory ability with an IC₅₀ value of 0.020 µM, similar to that of a reference drug safinamide (IC₅₀ = 0.019 µM) against MAO-B, followed by AC1 (IC₅₀ = 0.068 µM) and AC3 (IC₅₀ = 0.083 µM). Substituent -F in ring A (AC4) increased the MAO-B inhibition, followed by -H (AC1), -Br (AC3), and -Cl (AC2). The selectivity index (SI) value of AC4 was high (SI = 82.00) as well as other compounds (44.41 to 98.15). AC4 was found to be a reversible inhibitor as confirmed through analysis using the dialysis method. Interestingly, AC4 was observed to be a noncompetitive MAO-B inhibitor with a rare case and with K_i values of 0.011 ± 0.0036 µM. These experiments confirmed that AC4 is a reversible and potent selective inhibitor of MAO-B. Molecular docking experiments revealed that AC4 showed the highest inhibitory activity with a docking score (-9.510 kcal/mol). A study using molecular dynamics modeling revealed that the protein–ligand complex was more stable. It was observed that AC4 was non-cytotoxic in the study using L929 cell line. In conclusion, compound AC4 shows promise as a MAO-B inhibitor.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00929-z","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142045150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced extracellular production of maltotetraose amylase from Pseudomonas saccharophila in Bacillus subtilis through regulatory element optimization","authors":"Guilong Cong,&nbsp;Mingyu Li,&nbsp;Sitong Dong,&nbsp;Teng Ai,&nbsp;Xiaopeng Ren,&nbsp;Xianzhen Li,&nbsp;Conggang Wang,&nbsp;Fan Yang","doi":"10.1186/s13765-024-00921-7","DOIUrl":"10.1186/s13765-024-00921-7","url":null,"abstract":"<div><p>Maltotetraose amylase (Mta) catalyzes the hydrolysis of amylaceous polysaccharides into maltotetraose, which is an important functional sugar used in the food industry. However, the lack of efficient expression systems for recombinant Mta has hindered its scale-up production and application. In this study, a codon-optimized <i>mta</i> gene from <i>Pseudomonas saccharophila</i> was efficiently produced in <i>Bacillus subtilis</i> by optimizing the regulatory elements. First, a plasmid library containing 173 different signal peptide sequences placed upstream of <i>mta</i> gene was constructed, and transformed into <i>B. subtilis</i> strain WB800N(<i>amyE</i>Δ1) for high-throughput screening. The signal peptide yhcR was found to significantly enhance the secretion of Mta, reaching an activity of 75.4 U/mL in the culture medium. After optimization of the promoters, the Mta activity was further increased to 100.3 U/mL using a dual-promoter P_HpaIIP_amyE. Finally, the carbon sources and nitrogen sources for recombinant Mta production were optimized, yielding a highest Mta activity of 288.9 U/mL under the optimal culture conditions. The crude enzyme solution containing recombinant Mta produced a highest maltotetraose yield of 70.3% with 200 g/L of maltodextrin as the substrate. Therefore, the present study have demonstrated a high yield of Mta produced in <i>B. subtilis</i>, laying the foundation for large-scale Mta production and application.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00921-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142041501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-neuroinflammatory effects of the KIOM -patented Polygonum multiflorum maximized root tuber against LPS-stimulated BV2 Cells","authors":"Tae Woo Oh,&nbsp;Yeongjun Ban,&nbsp;Youngmin Kang","doi":"10.1186/s13765-024-00928-0","DOIUrl":"10.1186/s13765-024-00928-0","url":null,"abstract":"<div><p>The main pathological mechanism of neurodegeneration is neuroinflammation. It is known that the persistent neuroinflammatory response is harmful by causing secondary nerve tissue damage. Meanwhile, <i>P. multiflorum</i> is a traditional oriental medicinal herb. It has been used as a hematopoietic agent and is used to treat a variety of diseases and conditions. The aim of the present study was to compare the anti-inflammatory efficacy between the commonly available <i>P. multiflorum</i> (C1) and the KIOM-patented in vitro-propagated <i>P. multiflorum</i> (K1), which had higher content of active ingredients and biomass, using culture and cultivation conditions of LPS-induced neuroinflammation. After stimulation with LPS and treatment with C1 and K1 in mouse microglial BV-2 cells, nitric oxide (NO) production, pro-inflammatory cytokine secretion, inducible NO synthase (iNOS) expression, MAPK phosphorylation and transcription factor activity were assessed. We examined the antioxidant effect using DPPH and production of nitric oxide (NO). C1 and K1 suppressed the expression of iNOS and COX-2 and the production of pro-inflammatory cytokines. Furthermore, we determined the levels of inflammatory mediators, such as interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α and mitogen-activated protein kinases and IκBα via Western blotting to understand the regulating mechanisms. Additionally, C1 and K1 also inhibited the activation of p38 and nuclear factor-kappa B (NF-κB) in LPS-stimulated BV2 cells. In all experimental results, excellent anti-neuroinflammatory effects were confirmed at a lower dose in K1 than in C1, which is believed to be due to the increased biomass. Therefore, K1 is expected to be more effective than C1 and can be applied more broadly in the development of prevention and treatment of various inflammatory-mediated neurodegenerative diseases.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00928-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142013464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive analysis of a peripheral blood transcriptome signature in piglets infected with Salmonella Typhimurium: insight into immune responses","authors":"Eun-Yeong Bok,&nbsp;Seung-Won Yi,&nbsp;Han Gyu Lee,&nbsp;Jae Kyeom Kim,&nbsp;Kangwook Lee,&nbsp;Seungmin Ha,&nbsp;Bumseok Kim,&nbsp;Young-Hun Jung,&nbsp;Sang-Ik Oh","doi":"10.1186/s13765-024-00924-4","DOIUrl":"10.1186/s13765-024-00924-4","url":null,"abstract":"<div><p><i>Salmonella</i> Typhimurium (ST) infection in pigs poses a significant threat to animal health and food safety; the intricate mechanisms underlying host–immune responses and pathogen persistence remain poorly understood. To address this knowledge gap, we comprehensively analyzed the peripheral blood transcriptome in piglets infected with ST. We performed histopathological evaluation, blood parameter analysis, advanced RNA-sequencing techniques, and quantitative reverse transcription PCR (RT-qPCR)-based validation. The increasement in the monocyte counts at 2 days post-infection suggested its potential to serve as a hematological marker for ST infection in piglets. Functional and pathway enrichment analyses of the differentially expressed genes highlighted the pivotal roles of innate and adaptive immune responses, notably in pathways associated with Toll-like receptors, NIK/NF-κB signaling, cytokine signaling, and T cell proliferation. RT-qPCR-based validation using peripheral blood mononuclear cells provided additional insights into the immune system dynamics in response to ST infection, revealing the marked elevation of the interleukin (<i>IL</i>)<i>-15</i>, <i>IL-27</i>, and <i>CXCL10</i> levels being significantly elevated in ST-infected piglets. Our comprehensive analysis underscores the multifaceted impact of ST infection on piglets and offers valuable insights into the host–pathogen interactions and the role of host immune system during ST infection.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00924-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142013467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A study on the photoisomerization of phenylpropanoids and the differences in their radical scavenging activity using in-situ NMR spectroscopy and on-line radical scavenging activity analysis","authors":"Sangah Park,&nbsp;Hojin Kim,&nbsp;Miyeon Bang,&nbsp;Byung-Hun Um,&nbsp;Jin Wook Cha","doi":"10.1186/s13765-024-00925-3","DOIUrl":"10.1186/s13765-024-00925-3","url":null,"abstract":"<div><p>Phenylpropanoids are naturally occurring secondary metabolites that exhibit various biological activities such as ultra-violet (UV) light protection and reactive-oxygen species (ROS) scavenging. In this study, we utilized a light-emitting diode (LED) based in-situ UV irradiation nuclear magnetic resonance (NMR) technique to monitor the photoisomerization reactions of these phenylpropanoids under UV irradiation in real-time. Through this approach, we measured the photochemical reaction rates and photostationary state (PSS) ratios of these molecules and observed distinct reaction rate and PSS ratio information depending on the variation of substituent groups in each phenylpropanoid molecule. We also evaluated the radical scavenging activity (RSA) for each photochemical product through diphenyl-1-picrylhydrazyl radical (DPPH) assay and 2,2’-azino-bis(3-ethylbenzenthiazoline-6-sulphonic acid) (ABTS) assay. We found that the photoisomerization product of caffeic acid can increase both DPPH and ABTS radical scavenging activities, and confirmed the enhanced ABTS radical scavenging ability of caffeic acid <i>cis</i>-isomer based on the online high-pressure liquid chromatography (HPLC)-ABTS analysis and the PSS ratio information of each isomer.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00925-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141994036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic changes reveal hypoxic stress response in submerged seeds of maize (Zea mays L.)","authors":"Ji Won Kim,&nbsp;Seongmin Hong,&nbsp;Jiyun Go,&nbsp;Jin Seong Park,&nbsp;Gibum Yi","doi":"10.1186/s13765-024-00922-6","DOIUrl":"10.1186/s13765-024-00922-6","url":null,"abstract":"<div><p>Maize is highly sensitive to waterlogging stress, and seeds fail to germinate under hypoxic conditions induced by submergence, leading to severe yield losses. We conducted a comparative transcriptome analysis during the initial stages of seed germination, exploring aerobic and hypoxic conditions in two inbred lines, B73 and Okcheon Chal-1. Notably, significant differences emerged between aerobic and hypoxic conditions on the first day of germination, particularly in genes associated with fermentation and phytohormone regulation. However, consistent transcriptomic changes were observed in primary metabolic pathways such as glycolysis, the TCA cycle, and the pentose phosphate pathway. These differences strongly correlate with each other, illustrating the efficacy of the hypoxic response for survival in water. Furthermore, this suggests that germinating seeds serve as a promising model for studying plant hypoxia responses with controlled environmental conditions. Insights from this study contribute to understanding the fundamental mechanisms of hypoxia response and hold promise for developing strategies to cultivate waterlogging-tolerant maize cultivars.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"67 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-024-00922-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141994035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}