Applied Biological Chemistry最新文献

{"title":"Anti-cancer activity of rose-geranium essential oil and its bioactive compound geraniol in colorectal cancer cells","authors":"Hayeun Kim,&nbsp;Shiying Li,&nbsp;Sunita Nilkhet,&nbsp;Seung Joon Baek","doi":"10.1186/s13765-025-01005-w","DOIUrl":"10.1186/s13765-025-01005-w","url":null,"abstract":"<div><p>Essential oils are emerging as promising candidates in cancer therapeutics due to their various biological properties. This study investigates the anti-cancer effects of edible essential oils—Bergamot, Rose-geranium, Ginger, Turmeric, Myrrh, and Frankincense—on colorectal cancer cell lines, focusing on their impact on cell viability, molecular pathways, and anti-oxidant potential. Cell viability assays demonstrated dose-dependent inhibition of cancer cell growth, with rose-geranium essential oil showing the lowest IC50 values among those tested (0.37 µL/mL in HCT116 cells, 0.54 µL/mL in LoVo cells and 0.39 µL/mL in SW480 cells). Anti-oxidant activity showed that Ginger and Myrrh essential oils had the lowest EC50 values and highest Vitamin C Equivalent Antioxidant Capacity. Protein expression analysis revealed a significant decrease in oncogenic EpCAM protein and an increase in tumor suppressor proteins (p53 and NAG-1), with the most pronounced effects observed for rose-geranium oils. Geraniol, a primary component of rose-geranium essential oil, was further examined for its anti-cancer properties. Geraniol inhibited cell viability and spheroid formation in a dose-dependent manner, with significant effects on molecular markers such as NAG-1 and EpCAM expression. Antioxidant assays revealed that geraniol exhibited activity comparable to vitamin C. Furthermore, geraniol effectively enhanced NRF2-mediated antioxidant pathways, demonstrating comparable efficacy to the established NRF2 activator, quercetin. The transcript expression and promoter analyses demonstrated that geraniol regulates <i>NAG-1</i> transcription. Finally, the in silico analysis indicates that geraniol’s anti-cancer effects may be mediated by the modulation of transcription factors involved in <i>NAG-1</i> transcriptional regulation. These findings highlight the potential of edible essential oils, particularly rose-geranium and its active component geraniol, as promising therapeutic agents for colorectal cancer.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"68 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-025-01005-w","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145144126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regional and strain-level prevalence of nitrogen-fixing Bradyrhizobium with potential N2O reduction in South Korea","authors":"Jaeyoung Ro,&nbsp;Hor-Gil Hur,&nbsp;Sujin Lee","doi":"10.1186/s13765-025-00999-7","DOIUrl":"10.1186/s13765-025-00999-7","url":null,"abstract":"<div><p>Agricultural practices are the largest anthropogenic source of nitrous oxide (N₂O), a potent greenhouse gas contributing to global climate change. Applying symbiotic microbial inoculants capable of complete denitrification offers a promising strategy to mitigate N₂O emissions from agricultural fields. This study reports the strain-level diversity and geographical distribution of soybean symbiont bacteria <i>Bradyrhizobium</i> species carrying the <i>nosZ</i> gene, which encodes nitrous oxide reductase. Of 227 indigenous <i>Bradyrhizobium</i> isolates from soybean root nodules across South Korea, 162 were found to possess the <i>nosZ</i> gene, indicating their potential for N₂O reduction. The majority of the most prevalent species, <i>Bradyrhizobium diazoefficiens</i>, harbor the <i>nosZ</i> gene, contributing to the overall high frequency of <i>nosZ</i>-positive genotypes nationwide. In contrast, no evidence of the <i>nosZ</i> gene was detected in the second most abundant species, <i>Bradyrhizobium elkanii</i>, which was predominantly isolated from the southwestern regions, raising the possibility of elevated N₂O emissions in these areas. The presence of the <i>nosZ</i> gene varies substantially even within the same species, highlighting the importance of understanding strain-level genetic and functional diversity to develop <i>Bradyrhizobium</i> inoculants optimized for both nitrogen fixation and denitrification.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"68 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-025-00999-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145144265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alternate wetting and drying and nitrogen fertilizations under different soil conditions alter carbohydrate and nitrogen metabolisms in rice","authors":"Kyoung Rok Geem,&nbsp;Gahyun Kim,&nbsp;Jwakyung Sung","doi":"10.1186/s13765-025-01002-z","DOIUrl":"10.1186/s13765-025-01002-z","url":null,"abstract":"<div><p>Rice (<i>Oryza sativa</i> L.), a key staple crop, contributes over 20% of the daily caloric intake for more than 3.5 billion people worldwide. The continuous growth of the global population necessitates a steady increase in rice production, promoting advancement in agricultural cultivation systems to achieve both sustainability and productivity. Effective water and nitrogen (N) fertilizer management are crucial for optimizing rice productivity and ensuring environmental sustainability. This study investigates the interactive effects of alternate wetting and drying (AWD) irrigation and different nitrogen sources under contrasting soil conditions, focusing on nitrogen use efficiency (NUE), nitrogen metabolism, and carbohydrate metabolism. Our findings showed that soil type significantly influenced NUE and N metabolism depending on nitrogen fertilizers more than water management under ALN. However, under AHN, both nitrogen fertilizers and water management affected NUE and nitrogen metabolism. In acidic, low-nutrient soils (ALN), AWD decreased nitrogen uptake efficiency and downregulated the nitrogen transport and assimilation-related genes. Conversely, in alkaline, high-nutrient soils (AHN), AWD enhanced nitrogen uptake and assimilation, leading to increased upregulation of nitrogen transporter and assimilation genes. Additionally, AWD altered carbohydrate metabolism, increasing or decreasing carbohydrates accumulation by soil chemical and nutrient conditions. This study provides valuable insights into improving rice productivity by optimizing nitrogen use efficiency and promoting sustainability under various soil and water conditions.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"68 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-025-01002-z","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145144222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Facile preparation and activity of polymeric-composite-coated antimicrobial textile","authors":"Sangwon Ko,&nbsp;Jae-Young Lee,&nbsp;Duckshin Park,&nbsp;Kyunghoon Kim","doi":"10.1186/s13765-025-00994-y","DOIUrl":"10.1186/s13765-025-00994-y","url":null,"abstract":"<div><p>With the increasing demand for large-area antimicrobial textiles, particularly following the pandemic, it is crucial to develop cost-effective and facile textile coating methods. This study aimed to develop antimicrobial textiles using a polymeric composite applied through simple solution-coating methods, such as dipping and roll-to-roll processes. By incorporating a citric acid solubilizer and Cu(II) electrophiles, the antimicrobial effectiveness of chitosan-coated textiles was enhanced due to the synergistic effect of their inherent antimicrobial properties via the formation of crosslinked complexes. The chitosan–citrate–copper polymeric composites exhibited scalability for roll-to-roll PET coatings and over 99.9% antibacterial efficiency against <i>Escherichia coli</i> and <i>Staphylococcus aureus</i>. The roll-to-roll PET coating also showed significant effectiveness against Influenza A, reducing it by 99.895%. This facile antimicrobial coating process, which avoids chemical modification and prolonged immersion, offers advantages for the mass production of antimicrobial textiles in related industries.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"68 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-025-00994-y","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145142639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cellular enzyme-modulating activity of p-acetoxycinnamyl alcohol from Alpinia officinarum","authors":"Chang-Dae Lee,&nbsp;Hyosun Lim,&nbsp;Hak-Dong Lee,&nbsp;Sullim Lee,&nbsp;In Young Bae,&nbsp;Sanghyun Lee","doi":"10.1186/s13765-025-01004-x","DOIUrl":"10.1186/s13765-025-01004-x","url":null,"abstract":"<div><p><i>Alpinia officinarum</i>, commonly referred to as lesser galangal, is a medicinal plant traditionally used in East Asian practices owing to its anti-inflammatory, antioxidant, and antimicrobial properties. This study aimed to isolate <i>p</i>-acetoxycinnamyl alcohol from <i>A. officinarum</i> and evaluate its effect on melanogenesis-related enzyme expression. <i>p</i>-Acetoxycinnamyl alcohol was successfully isolated and structurally characterized from <i>A. officinarum</i> using NMR spectroscopy. The compound’s skin-whitening potential was investigated through a combination of molecular docking simulations and in vitro assays using B16F10 melanoma cells. The results demonstrated that <i>p</i>-acetoxycinnamyl alcohol showed potent binding affinity and inhibited the expression of microphthalmia-associated transcription factor, tyrosinase, tyrosinase-related protein (TRP)-1, and TRP-2 in melanocytes as shown by molecular docking simulations and in vitro assays. These findings indicate that <i>p</i>-acetoxycinnamyl alcohol has potential as a natural skin-whitening compound that controls melanogenic enzyme expression and functionality. The therapeutic potential of <i>A. officinarum</i> in functional cosmeceuticals and dermatological therapies emphasizes its pharmacognostical importance.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"68 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-025-01004-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145145272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomarkers for biological quality control of Samsoeum: a scoping review","authors":"Mei Tong He,&nbsp;Ji Hwan Lee,&nbsp;Noriko Yamabe,&nbsp;Kiwon Jung,&nbsp;Ki Sung Kang","doi":"10.1186/s13765-025-01000-1","DOIUrl":"10.1186/s13765-025-01000-1","url":null,"abstract":"<div><p>Samsoeum<b> (</b>SSE) is a traditional herbal prescription used to cure early signs of a cold such as headache, cough, runny nose, and fever. The safety and efficacy of SSE can be guaranteed with the use of its quality control biomarkers. Although chemical quality control is performed to ensure consistent efficacy of SSE, it has limitations in evaluating the efficacy of complex natural products. To find biological assays that could complement the biological quality control of SSE, we conducted a study of the literature. In this review, we collected and identified 17 articles that investigated the efficacy of SSE, including clinical, in vivo, and in vitro studies. As a result, most studies evaluated anti-inflammatory and anti-allergy efficacies. One clinical study has reported efficacy of SSE for chronic bronchitis. In basic studies, 7 in vivo studies and 4 in vitro studies were mainly related to chronic diseases, including inflammation, allergy, cancer, and obesity. Optimal in vitro and in vivo efficacy evaluation techniques that can assess consistent clinical effects should be chosen for future qualitative evaluation studies of herbal prescriptions (herbal medicines).</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"68 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-025-01000-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144140086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resveratrol protects against uremic serum-induced endothelial cell injury by activating the FUS/KLF2/FBXW7 signaling pathway","authors":"Danjun Wang,&nbsp;Jianlian Liu,&nbsp;Juan Wang,&nbsp;Yang Feng","doi":"10.1186/s13765-025-00997-9","DOIUrl":"10.1186/s13765-025-00997-9","url":null,"abstract":"<div><h3>Background</h3><p>Chronic kidney disease causes endothelial cell dysfunction associated with uremia, which triggers a high risk of cardiovascular diseases. Furthermore, prolonged exposure of the vascular endothelium to uremic toxins could provoke endothelial damage in patients with end-stage renal disease. Resveratrol (RSV), a dietary polyphenol compound, has been reported to possess health benefits due to its anti-inflammatory and anti-oxidative properties. However, the role of RSV on uremic serum (US)-induced endothelial cell injury is still unclear.</p><h3>Methods</h3><p>HUVECs were stimulated by the US to mimic the inflammatory damage model in vitro. Cell viability and apoptosis were detected using CCK-8 and flow cytometry. IL-6, IL-1β, and TNF-α were evaluated using ELISA. ROS and SOD levels were detected using special assay kits. Kruppel-Like Factor 2 (KLF2), Fused-in-Sarcoma (FUS), and F-box and WD repeat domain-containing 7 protein (FBXW7) levels were determined using western blot. KLF2 mRNA level was examined using RT-qPCR. After ENCORI, HitPredict, and BioGRID software prediction, the interaction between KLF2 and FUS or FBXW7 was identified using RIP and Co-Immunoprecipitation (IP) assays.</p><h3>Results</h3><p>RSV could relieve US-triggered HUVEC viability inhibition, apoptosis, inflammatory response, and oxidative stress promotion. KLF2 knockdown partly attenuated the repression of RSV on US-induced HUVEC injury. Mechanistically, FUS bound with KLF2 to improve the stability of KLF2 mRNA. KLF2 interacted with FBXW7. RSV hindered US-caused HUVEC injury by regulating FUS/KLF2/FBXW7 pathway.</p><h3>Conclusion</h3><p>RSV exposure could mitigate US-evoked HUVEC dysfunction by activating the FUS/KLF2/FBXW7 pathway, providing a better understanding of the role of RSV in the anti-inflammatory therapeutics for uremia treatment.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"68 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-025-00997-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144125525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of residue dissipation of hexaconazole in Chinese cabbage and spring scallion","authors":"Ji Won Lee,&nbsp;Jin-Seong Kim,&nbsp;Ji Hyun Park,&nbsp;Hyun Ho Noh,&nbsp;Min Seok Oh,&nbsp;Jin-Hyo Kim,&nbsp;Kyeong-Ae Son","doi":"10.1186/s13765-025-00995-x","DOIUrl":"10.1186/s13765-025-00995-x","url":null,"abstract":"<div><p>The characterization of hexaconazole residues was evaluated through both single and sequential applications in Chinese cabbage and spring scallion. Maximum residue levels following foliar application were determined to be 1.14 mg kg^− 1 in the cabbage and 0.04 mg kg^− 1 in the scallion, whereas the soil application contributed minimally. Notably, the initial residue in the cabbage was 28 times greater than that observed in the scallion. Fourteen days after the final foliar application, the cabbage exhibited a 3.6-fold increase in fresh weight, compared to a 1.4-fold increase in the scallion. The degradation rates of hexaconazole were calculated at 0.089 day^− 1 for the cabbage and 0.207 day^− 1 for the scallion, corresponding with degradation half-lives (T_1/2) of 7.8 days for the cabbage, which was more than double the 3.4 days for the scallion. Biological T_1/2s in vitro were 210 min for the scallion, while the degradation was not detected in the cabbage. Finally, the 50% dissipation times (DT₅₀) were 3.6–3.8 days for the cabbage and 2.0-3.7 days for the scallion after foliar and sequential applications. In addition, hexaconazole exhibited persistence in soil under greenhouse conditions, resulting in the translocation of residues to rotational crops with detected concentrations reaching up to 0.013 mg kg^− 1.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"68 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-025-00995-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144117651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The antioxidant activity of diosgenin, a plant steroid sapogenin, in C2C12 myoblasts is achieved by blocking mitochondrial ROS production","authors":"Cheol Park,&nbsp;Gi-Young Kim,&nbsp;Yung Hyun Choi","doi":"10.1186/s13765-025-00996-w","DOIUrl":"10.1186/s13765-025-00996-w","url":null,"abstract":"<div><p>Diosgenin, a plant-derived steroid sapogenin, has been reported to have many health benefits, including antioxidant activity. Although oxidative stress is a major factor impeding the differentiation and homeostasis of skeletal muscles, its antioxidant activity in skeletal muscle cells has not been thoroughly studied. This study aimed to explore the protective mechanisms of diosgenin against oxidative damage in skeletal muscle cells. C2C12 murine myoblasts were pretreated with nontoxic concentrations of diosgenin and exposed to hydrogen peroxide (H₂O₂) to mimic oxidative stress. The results of this study showed that diosgenin significantly reduced H₂O₂-induced cytotoxicity, blocked the formation of comet tails, and increased the levels of 8-hydroxy-2’-deoxyguanosine, which are representative biomarkers of DNA damage. In addition, diosgenin counteracted H₂O₂-induced apoptosis by enhancing the Bax/Bcl-2 expression ratio and suppressing the activation of the caspase cascade, which is associated with the blockade of cytochrome <i>c</i> release into the cytoplasm by maintaining mitochondrial stability. Furthermore, diosgenin eliminated the production of intracellular and mitochondrial reactive oxygen species (ROS) by restoring glutathione (GSH) content and the activities of antioxidant enzymes such as GSH peroxidase 1 and manganese-dependent superoxide dismutase, which were inhibited by H₂O₂. Therefore, diosgenin protects C2C12 myoblasts from oxidative damage by attenuating mitochondrial ROS generation and regulating the mitochondrial apoptotic pathway.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"68 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-025-00996-w","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144117788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"7,8-dihydroxyflavone and daphnetin have virucidal and neuraminidase inhibition activities against influenza A virus in vitro","authors":"Sujeong Kim,&nbsp;Ju Won Kim,&nbsp;Sanghyun Lee,&nbsp;Yo Han Jang","doi":"10.1186/s13765-025-00998-8","DOIUrl":"10.1186/s13765-025-00998-8","url":null,"abstract":"<div><p>Influenza A virus remains a major global health concern, underscoring the need for novel antiviral agents. This study investigated the antiviral potential of two natural compounds, 7,8-dihydroxyflavone (DHF) and daphnetin (DAP), against influenza A viruses in vitro. Following in vitro cytotoxicity and antioxidative activity assessments, the antiviral effects of DHF and DAP were evaluated, with a particular focus on their direct viral inhibition. DHF and DAP demonstrated complete virucidal activity influenza A virus at concentrations of 50 µM and 100 µM, respectively. However, neither compound inhibited influenza surface protein hemagglutination (HA), suggesting that their virucidal effects are independent of HA receptor binding. Both compounds exhibited neuraminidase (NA) inhibition, with DAP showing stronger activity compared to DHF. Furthermore, DHF and DAP suppressed influenza virus replication in cells, as evidenced by a reduction in green fluorescence protein (GFP) reporter expression in virus-infected cells. Growth kinetics analysis revealed that both compounds significantly reduced viral replication when applied to cells before or after viral infection. These findings demonstrate that DHF and DAP exhibit multifaceted antiviral activity, including direct virucidal action, NA inhibition, and suppression of viral replication. Our results suggest that DHF and DAP are promising candidates for the development of novel influenza therapeutics.</p></div>","PeriodicalId":467,"journal":{"name":"Applied Biological Chemistry","volume":"68 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://applbiolchem.springeropen.com/counter/pdf/10.1186/s13765-025-00998-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144100276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}