Applied Biochemistry and Microbiology最新文献_第7页

Comparison of Efficiency of Different Promoters by β-Carotene Production in the Yeast Yarrowia lipolytica 脂性耶氏酵母不同启动子生产β-胡萝卜素效率的比较

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2026-02-24 DOI: 10.1134/S0003683825603762

A. A. Cherenkova, M. O. Taratynova, O. E. Melkina

{"title":"Comparison of Efficiency of Different Promoters by β-Carotene Production in the Yeast Yarrowia lipolytica","authors":"A. A. Cherenkova, M. O. Taratynova, O. E. Melkina","doi":"10.1134/S0003683825603762","DOIUrl":"10.1134/S0003683825603762","url":null,"abstract":"In the metabolic engineering of producer strains, strong constitutive promoters are an attractive alternative to inducible promoters, since they do not require the addition of expensive and sometimes toxic inducing substances to the fermentation medium. In this work, we have compared the Yarrowia lipolytica constitutive promoters: pEXP1, pRPS2, pRPL22 (native) and hybRPS2_499, hybRPL22 (synthetic) with the inducible promoter pEYK1-3AB. The synthetic promoters hybRPS2 and hybRPL22 are modified versions of the ribosomal promoters pRPS2 and pRPL22, respectively. Initially, promoter strength was assessed by the expression level of the reporter gene encoding the green fluorescent protein hrGFP. It was shown that the strength of the promoters increased in the following order: pRPS2, pRPL22, pEXP1, hybRPL22, hybRPS2_499, while the level of pEYK1-3AB activity with 1.5% erythritol induction was comparable to the level of unmodified pRPS2 and pRPL22. During heterologous expression of the CarRP-GGPPSs7 genes under the control of the studied promoters, the highest β-carotene production was observed in the recombinant Y. lipolytica strain with the pEXP1 promoter, while the hybRPS2_499 promoter showed the result comparable to the pEYK1-3AB promoter induced by the addition of 1.5% erythritol. Thus, we have shown that in constructing heterologous biosynthetic pathways in Y. lipolytica, a strong constitutive promoter can successfully compete with an inducible variant. However, in our case, the use of the strongest promoter did not lead to the highest yield of the target product. Therefore, rapid identification of the best producer strain requires testing a wide range of promoters of varying strengths.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"62 1","pages":"107 - 115"},"PeriodicalIF":1.1,"publicationDate":"2026-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1134/S0003683825603762.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147341434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Activation of Biosynthesis of Diethylhexyl Phthalate in Wheat during Germination: Possible Physiological Functions 小麦萌发过程中邻苯二甲酸二乙己酯生物合成的激活：可能的生理功能

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2026-02-24 DOI: 10.1134/S0003683825600460

A. G. Enikeev, V. A. Kustov, A. V. Permyakov, A. A. Semenov

引用次数: 0

Development of Immunochromatographic Assay of the Anti-Mullerian Hormone by Surface-Enhanced Raman Scattering Using Bimetallic Nanoparticles 双金属纳米粒子表面增强拉曼散射免疫层析法测定抗苗勒管激素的研究进展

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2026-02-24 DOI: 10.1134/S0003683825602240

K. V. Serebrennikova, N. S. Komova, A. V. Zherdev, B. B. Dzantiev

{"title":"Development of Immunochromatographic Assay of the Anti-Mullerian Hormone by Surface-Enhanced Raman Scattering Using Bimetallic Nanoparticles","authors":"K. V. Serebrennikova, N. S. Komova, A. V. Zherdev, B. B. Dzantiev","doi":"10.1134/S0003683825602240","DOIUrl":"10.1134/S0003683825602240","url":null,"abstract":"The use of surface-enhanced Raman spectroscopy (SERS) in immunochromatographic assay (ICA) supports integration of the rapidity, simplicity, and specificity of the immunoassay with high sensitivity and accuracy of SERS registration. An immunochromatographic test system has been developed for detection of the anti-Müllerian hormone (AMH), a marker of ovarian reserve in women of reproductive age. ICA has been implemented in a sandwich format, which facilitates the interaction between the AMH molecules in the tested sample and a pair of monoclonal antibodies that recognise different antigen epitopes. This interaction results in the formation of AMH-labeled antibody complexes (where the antibody is immobilized on the membrane of the immunochromatographic test strip). We have used bimetallic gold–silver core-shell nanoparticles modified with a SERS-active reporter molecule to label antibodies. We have evaluated the size characteristics of the nanoparticles and selected the optimal conditions for their conjugation with antibodies. ICA has been performed under conditions that ensure the achievement of the minimum AMH detection limit. The developed ICA-SERS system has a detection limit of 0.15 ng/mL and a working range of 4.7–29.5 ng/mL satisfying practical diagnostic requirements. The analytical system has been applied successfully to testing blood serum.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"62 1","pages":"180 - 189"},"PeriodicalIF":1.1,"publicationDate":"2026-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147341426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Prospects of an Acoustic Sensor System for Indication of Cancer Cells with Localized Heat Shock Proteins in Conducting Suspensions 用传导悬浮液中局部热休克蛋白指示癌细胞的声传感器系统的前景

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2026-02-24 DOI: 10.1134/S0003683825603208

O. I. Guliy, B. D. Zaitsev, S. A. Staroverov, I. A. Borodina

引用次数: 0

The Role of Anaplerotic Reactions for Oxaloacetate Synthesis in the L-Lysine-Producing Strain of Corynebacterium glutamicum 谷氨酸棒状杆菌l -赖氨酸生产菌株合成草酰乙酸的倒转反应作用

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2026-02-24 DOI: 10.1134/S0003683825603385

T. E. Leonova, L. E. Ryabchenko, T. I. Kalinina, T. V. Gerasimova, A. S. Yanenko

{"title":"The Role of Anaplerotic Reactions for Oxaloacetate Synthesis in the L-Lysine-Producing Strain of Corynebacterium glutamicum","authors":"T. E. Leonova, L. E. Ryabchenko, T. I. Kalinina, T. V. Gerasimova, A. S. Yanenko","doi":"10.1134/S0003683825603385","DOIUrl":"10.1134/S0003683825603385","url":null,"abstract":"The properties of three mutants of the industrial-scale L-lysine–producing strain Corynebacterium glutamicum VKPM B-11969 were studied. These mutants had one of the anaplerotic pathways for synthesizing lysine precursors, oxaloacetate, blocked due to inactivation of the ppc (encodes phosphoenolpyruvate carboxylase) or pyc (encodes pyruvate carboxylase) genes, or one of the pyruvate synthesis pathways was disrupted due to inactivation of the pyk gene (encodes pyruvate kinase). Inactivation of pyruvate carboxylase, which catalyzes the synthesis of oxaloacetate from pyruvate and CO2, did not affect L-lysine production. Inactivation of pyruvate kinase, which catalyzes the synthesis of pyruvate from phosphoenolpyruvate, resulted in a decrease in the rate of L-lysine synthesis (by 20–25%). The synthesis of L-lysine decreased to the greatest extent with the inactivation of phosphoenolpyruvate carboxylase, by 30–50% depending on the growing conditions. It was concluded that, in the B-11969 strain, under conditions of active synthesis of L‑lysine, the formation of oxaloacetate occurs predominantly with the participation of phosphoenolpyruvate carboxylase. However, the increase in the expression of the ppc gene in the producer strain led to an increase in the activity of phosphoenolpyruvate carboxylase, but not in the production of L-lysine, which allowed us to assume that in the B-11969 strain the stage of oxaloacetate synthesis is not limiting for the production of lysine.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"62 1","pages":"29 - 38"},"PeriodicalIF":1.1,"publicationDate":"2026-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1134/S0003683825603385.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147341421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multispecies Biofilms of PGPR Strains with Antibacterial Potential as a Basis for the Development of Effective Biopreparations 具有抗菌潜力的PGPR菌株的多物种生物膜是开发有效生物修复的基础

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2026-02-24 DOI: 10.1134/S0003683825602999

A. M. Lavina, A. A. Vladimirova, An. Kh. Baimiev, Al. Kh. Baimiev

引用次数: 0

Prospects for the Use of Plant-Associated Bacteria in Biological Control of Oomycetes 植物伴生菌在卵菌生物防治中的应用前景

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2026-02-24 DOI: 10.1134/S000368382560318X

A. V. Sorokan, V. F. Gabdrakhmanova, I. V. Maksimov

{"title":"Prospects for the Use of Plant-Associated Bacteria in Biological Control of Oomycetes","authors":"A. V. Sorokan, V. F. Gabdrakhmanova, I. V. Maksimov","doi":"10.1134/S000368382560318X","DOIUrl":"10.1134/S000368382560318X","url":null,"abstract":"Oomycetes are a particularly dangerous group of pathogens that significantly reduce both the productivity and the quality of production of agricultural crops. Protective measures using available fungicides are often ineffective against them. One of the simplest and, at the same time, innovative approaches to plant protection from oomycete infection can be considered biological methods, which, if used in a timely manner, can be even more effective than chemical means of protection. Biological control involves the use of beneficial microorganisms that can stop the development of diseases by producing biologically active substances with antibiotic activity, such as antibiotics, biosurfactants, and toxins. In this review, we describe a strategy for sustainable prevention and control of oomycete pathogens using plant-associated microorganisms, including bacteria of the genera Bacillus, Paenibacillus, Streptomyces, Pseudomonas, and Lactobacillus. The mechanisms that determine the protective effects of bioprotective strains in the endo-, phyllo-, and rhizosphere of plants are also considered, which can be used in the development of strategies for constructing phytomicrobiomes.","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"62 1","pages":"1 - 16"},"PeriodicalIF":1.1,"publicationDate":"2026-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147341433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Erratum to: Glycation of Leghemoglobin by Methylglyoxal in Comparison with Other Hemoglobins and the Influence on Peroxidase Activity 与其他血红蛋白相比，甲基乙二醛使豆红蛋白糖化及其对过氧化物酶活性的影响

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2026-01-16 DOI: 10.1134/S0003683825090108

E. I. Nasybullina, O. V. Kosmachevskaya, A. F. Topunov

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Erratum to: Effect of Fermentation with Different Lactobacilli on the Organoleptic Properties of Pea Protein Isolate 不同乳酸菌发酵对豌豆分离蛋白感官特性的影响

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2026-01-16 DOI: 10.1134/S0003683825090042

I. V. Kravchenko, V. A. Furalyov, E. S. Pshennikova, A. N. Fedorov, V. O. Popov

引用次数: 0

A New Relevant Cellular Model of Uterine Leiomyoma: Method of Isolation and Prospects of Use 一种新的子宫平滑肌瘤相关细胞模型：分离方法及应用前景

IF 1.1 4区生物学

Applied Biochemistry and Microbiology Pub Date : 2026-01-16 DOI: 10.1134/S0003683825700553

N. Yu. Shved, A. A. Egorova, S. V. Shtykalova, N. S. Osinovskaya, I. Yu. Kogan, A. V. Kiselev

引用次数: 0