{"title":"Influence of a Wood Substrate on the Profile of Volatile Organic Compounds Produced by White Rot Fungus Trametes hirsuta LE-BIN 072","authors":"A. V. Shabaev, T. V. Fedorova","doi":"10.1134/S0003683824605821","DOIUrl":null,"url":null,"abstract":"<p>Volatilomes of the basidial wood-destroying white rot fungus <i>Trametes hirsuta</i> LE-BIN 072 growing on a glucose–peptone medium (GP) with different types of wood sawdust (birch, alder, and pine) are represented by the following main groups of compounds: terpenes, aromatic compounds, fatty acid derivatives, and alkanes. The addition of sawdust to the cultivation medium led to a change in the spectrum of terpene and sesquiterpene compounds synthesized by fungus, as well as to a change in the ratio of the VOC component composition. On the control GP medium, β-elemene, β-barbatene, and γ-muurolene were detected; on the media with birch, alder, and pine sawdust, α-bulnesene, γ-cadinene, Δ3-carene, and camphene were detected. In silico analysis of the <i>T. hirsuta</i> 072 genome predicted ten genes encoding terpene synthases. Phylogenetic analysis showed that the proteins clustered with other basidiomycete terpene synthases into four major clades, suggesting that the enzymes in each cluster may produce related terpenes and sesquiterpenes.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 6","pages":"1240 - 1251"},"PeriodicalIF":1.0000,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied Biochemistry and Microbiology","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1134/S0003683824605821","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Volatilomes of the basidial wood-destroying white rot fungus Trametes hirsuta LE-BIN 072 growing on a glucose–peptone medium (GP) with different types of wood sawdust (birch, alder, and pine) are represented by the following main groups of compounds: terpenes, aromatic compounds, fatty acid derivatives, and alkanes. The addition of sawdust to the cultivation medium led to a change in the spectrum of terpene and sesquiterpene compounds synthesized by fungus, as well as to a change in the ratio of the VOC component composition. On the control GP medium, β-elemene, β-barbatene, and γ-muurolene were detected; on the media with birch, alder, and pine sawdust, α-bulnesene, γ-cadinene, Δ3-carene, and camphene were detected. In silico analysis of the T. hirsuta 072 genome predicted ten genes encoding terpene synthases. Phylogenetic analysis showed that the proteins clustered with other basidiomycete terpene synthases into four major clades, suggesting that the enzymes in each cluster may produce related terpenes and sesquiterpenes.
期刊介绍:
Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.