APL Bioengineering最新文献_第3页

ALK5 inhibitor impact on bleomycin-induced systemic sclerosis mouse model via multifunctional optical coherence tomography. ALK5抑制剂对博莱霉素诱导的系统性硬化症小鼠模型的影响

IF 4.1 3区医学

APL Bioengineering Pub Date : 2025-11-06 eCollection Date: 2025-12-01 DOI: 10.1063/5.0281447

Pavel V Nikitin, Harshdeep S Chawla, Jessica Gutierrez, Geethangili Madamanchi, Manmohan Singh, Salavat R Aglyamov, Minghua Wu, Jerry Alonso, Matthew Fronheiser, Juliana Coculo, Shuyan Du, Shervin Assassi, Kirill V Larin

{"title":"ALK5 inhibitor impact on bleomycin-induced systemic sclerosis mouse model via multifunctional optical coherence tomography.","authors":"Pavel V Nikitin, Harshdeep S Chawla, Jessica Gutierrez, Geethangili Madamanchi, Manmohan Singh, Salavat R Aglyamov, Minghua Wu, Jerry Alonso, Matthew Fronheiser, Juliana Coculo, Shuyan Du, Shervin Assassi, Kirill V Larin","doi":"10.1063/5.0281447","DOIUrl":"10.1063/5.0281447","url":null,"abstract":"Systemic sclerosis (SSc) is a chronic autoimmune disease characterized by fibrosis, vascular dysfunction, and immune dysregulation, leading to significant morbidity and mortality. Noninvasive imaging techniques are critical for monitoring disease progression and evaluating therapeutic interventions. This study investigates the technical feasibility of multifunctional optical coherence tomography (OCT)-based methods for longitudinal assessment of skin thickness, stiffness, and microvasculature in a murine SSc model as a translational, noninvasive, and quantitative method to study disease progression and treatment response. Our findings demonstrate significant structural, biomechanical, and vascular changes in the skin's stiffness, indicative of fibrosis, a hallmark of SSc. The application of SB 525334 (a transforming growth factor β1 receptor ALK5 inhibitor) mitigated these changes, highlighting its potential as a treatment strategy. Despite the inherent limitations of the mouse model in replicating the complexity of SSc, this study introduces a new technique for investigating the SSc pathogenesis and evaluating the efficacy of potential SSc therapies. These results encourage further exploration of the multifunctional Optical Coherence Elastography and OCT Angiography for monitoring disease progression and treatment response in SSc. In summary, bleomycin treatment significantly increased skin thickness, stiffness, and vessel lumen width, while SB 525334 partially reversed these changes, demonstrating the feasibility of our multifunctional OCT approach for monitoring experimental SSc.","PeriodicalId":46288,"journal":{"name":"APL Bioengineering","volume":"9 4","pages":"046110"},"PeriodicalIF":4.1,"publicationDate":"2025-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12594547/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145483415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergy between photonics and biological approaches-A review of combination therapies for cancer theranostics. 光子学与生物学方法的协同作用——癌症治疗联合疗法综述。

IF 4.1 3区医学

APL Bioengineering Pub Date : 2025-10-29 eCollection Date: 2025-12-01 DOI: 10.1063/5.0252574

Jacob P Adams, Luke Pauli, Lin Wang, Trisha Valerio, Coline Furrer, Min Li, Naoko Takebe, Joanne Tuohy, Wei R Chen

{"title":"Synergy between photonics and biological approaches-A review of combination therapies for cancer theranostics.","authors":"Jacob P Adams, Luke Pauli, Lin Wang, Trisha Valerio, Coline Furrer, Min Li, Naoko Takebe, Joanne Tuohy, Wei R Chen","doi":"10.1063/5.0252574","DOIUrl":"10.1063/5.0252574","url":null,"abstract":"Cancer therapies have evolved considerably over the past several decades. Physical interactions, particularly through phototherapy as a new class of treatment modality, have become widely used. Phototherapy utilizes light to combat cancer by generating heat (photothermal therapy), reactive oxygen species (photodynamic therapy), or photochemical internalization, to kill targeted cells. While these therapies have shown promise in pre-clinical studies, they have demonstrated limitations in clinical cancer treatment. Primarily, while phototherapies excel in eliminating primary tumors, they often fail to provide systemic effects, particularly when treating metastatic cancers, in addition to certain undesirable side effects. Phototherapy has been combined with immunotherapy, nanomedicine, and other cancer therapies to overcome specific weaknesses and enhance the therapeutic benefits of individual treatments. These combinations often involve nanomaterials to deliver adjuncts for phototherapy to the tumor site, immune stimulants/adjuvants to enhance the immune response, and immune checkpoint inhibitors to counter immune suppression. Phototherapies may also be combined with specific photonics-related principles to enable simultaneous diagnostic and therapeutic effects, known as theranostics. Herein, we review current approaches to modern cancer therapies, such as nanotechnology-based therapies, immunotherapies, and especially phototherapy, and the combination of these therapies with diagnostic techniques in combating cancer.","PeriodicalId":46288,"journal":{"name":"APL Bioengineering","volume":"9 4","pages":"041503"},"PeriodicalIF":4.1,"publicationDate":"2025-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12575004/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145432506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Subject-specific multivariate modeling for regenerative rehabilitation of bone healing. 骨愈合再生康复的多变量模型。

IF 4.1 3区医学

APL Bioengineering Pub Date : 2025-10-24 eCollection Date: 2025-12-01 DOI: 10.1063/5.0273944

Kylie E Williams, Farhan Muhib, Ethan Dinh, Kelly E Leguineche, Auveen Hajarizadeh, J Walker Rosenthal, Tyler Guyer, Theo Seah, Nick J Willett, Jeffrey A Weiss, Robert E Guldberg

{"title":"Subject-specific multivariate modeling for regenerative rehabilitation of bone healing.","authors":"Kylie E Williams, Farhan Muhib, Ethan Dinh, Kelly E Leguineche, Auveen Hajarizadeh, J Walker Rosenthal, Tyler Guyer, Theo Seah, Nick J Willett, Jeffrey A Weiss, Robert E Guldberg","doi":"10.1063/5.0273944","DOIUrl":"10.1063/5.0273944","url":null,"abstract":"Rehabilitation can help promote functional restoration following surgical reconstruction of severe bone injuries, yet prescribed regimens are often conservative due to limited understanding of their impact on healing. This study examined rodent physical activity parameters, including wheel running duration, distance, bout frequency, bout duration, velocity, and rest time, assessing their combined impact on bone healing in 2 and 3 mm segmental defects. Artifical intelligence (AI)-based genetic programming generated high-accuracy nonlinear models, revealing a \"goldilocks\" phenomenon: some rehabilitation stimulates bone formation, but excessive activity is detrimental. Subject-specific finite element modeling showed that local defect compressive strains decreased only in injuries that achieved union and that the correlation between strain and healing evolved over time. These findings highlight the dynamic healing process, necessitating a subject-specific approach. While bone healing is often thought to be primarily driven by achieving optimal strain magnitudes, our results suggest a more complex reality. Bone healing depends not only on optimal strain magnitude but also on balancing activity and rest, which shifts with injury severity and healing progression. Overall, effective rehabilitation must consider injury stabilization, severity, and healing status while ensuring adequate rest to promote optimal bone regeneration.","PeriodicalId":46288,"journal":{"name":"APL Bioengineering","volume":"9 4","pages":"046107"},"PeriodicalIF":4.1,"publicationDate":"2025-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12553521/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145379127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessing human scalp and brain blood flow sensitivities via superficial temporal artery occlusion using speckle contrast optical spectroscopy. 利用散斑对比光谱学通过颞浅动脉闭塞评估人头皮和脑血流敏感性。

IF 4.1 3区医学

APL Bioengineering Pub Date : 2025-10-21 eCollection Date: 2025-12-01 DOI: 10.1063/5.0263953

Yu Xi Huang, Simon Mahler, Maya Dickson, Aidin Abedi, Yu Tung Lo, Patrick D Lyden, Jonathan Russin, Charles Liu, Changhuei Yang

{"title":"Assessing human scalp and brain blood flow sensitivities via superficial temporal artery occlusion using speckle contrast optical spectroscopy.","authors":"Yu Xi Huang, Simon Mahler, Maya Dickson, Aidin Abedi, Yu Tung Lo, Patrick D Lyden, Jonathan Russin, Charles Liu, Changhuei Yang","doi":"10.1063/5.0263953","DOIUrl":"10.1063/5.0263953","url":null,"abstract":"Cerebral blood flow is a critical metric for cerebrovascular monitoring, with applications in stroke detection, brain injury evaluation, aging, and neurological disorders. Noninvasively measuring cerebral blood dynamics is challenging due to the presence of scalp and skull, which obstruct direct brain access and contain their own blood dynamics that must be isolated. We developed an aggregated seven-channel speckle contrast optical spectroscopy (SCOS) system to measure blood flow and blood volume noninvasively. Each channel, with a distinct source-to-detector distance, targeted different depths to detect scalp and brain blood dynamics separately. By briefly occluding the superficial temporal artery, which supplies blood only to the scalp, we isolated surface blood dynamics from brain signals. Results on 20 subjects show that scalp-sensitive channels experienced significant reductions in blood dynamics during occlusion, while brain-sensitive channels experienced minimal changes. This provides experimental evidence of scalp blood flow sensitivity in diffuse optical measurements such as SCOS, highlighting optimal configuration for preferentially probing brain signals noninvasively.","PeriodicalId":46288,"journal":{"name":"APL Bioengineering","volume":"9 4","pages":"046106"},"PeriodicalIF":4.1,"publicationDate":"2025-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12543367/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145356396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Credibility assessment of patient-specific modeling in transcatheter aortic valve implantation. I. A population-based validation of patient-specific modeling. 经导管主动脉瓣植入术中患者特异性建模的可信度评估。1 .基于人群的患者特异性模型验证。

IF 4.1 3区医学

APL Bioengineering Pub Date : 2025-09-25 eCollection Date: 2025-12-01 DOI: 10.1063/5.0280958

Chiara Catalano, Roberta Scuoppo, Tahir Turgut, Vincent Bouwman, Nils Götzen, Stefano Cannata, Giovanni Gentile, Caterina Gandolfo, Salvatore Pasta

{"title":"Credibility assessment of patient-specific modeling in transcatheter aortic valve implantation. I. A population-based validation of patient-specific modeling.","authors":"Chiara Catalano, Roberta Scuoppo, Tahir Turgut, Vincent Bouwman, Nils Götzen, Stefano Cannata, Giovanni Gentile, Caterina Gandolfo, Salvatore Pasta","doi":"10.1063/5.0280958","DOIUrl":"10.1063/5.0280958","url":null,"abstract":"Transcatheter aortic valve implantation (TAVI) has become a standardized treatment for aortic valve stenosis, supported by computational modeling to enhance procedural planning. However, the credibility of TAVI simulations requires rigorous validation following regulatory standards. This study aims to perform a population-based validation of the structural and hemodynamic simulation performance of the SAPIEN 3 (S3) Ultra device by comparing computational predictions with clinical post-TAVI data, following the ASME V&V 40 framework. A patient-specific structural model followed by fluid-structure interaction was developed to simulate S3 deployment and then assess post-TAVI hemodynamics in 20 patients. Structural parameters (device diameters) and hemodynamic indices (effective orifice area, EOA, and transmural pressure gradient, TPG) were extracted. Validation was performed using empirical cumulative distribution function (ECDF) analysis, with an acceptance threshold of 5% for model credibility. EOA and TPG predictions showed reasonable agreement with echocardiographic data (errors within 10%). ECDF-based comparison demonstrated a high level of accuracy for device diameters (≤5% area metric), whereas hemodynamic parameters exhibited slightly greater discrepancies, potentially due to clinical measurement variability. This study establishes a robust computational validation framework for patient-specific TAVI modeling, ensuring regulatory compliance and clinical applicability. These findings highlight the potential of in silico trials to support TAVI planning and decision-making. This study is complemented by a second part dedicated to uncertainty quantification and sensitivity analysis.","PeriodicalId":46288,"journal":{"name":"APL Bioengineering","volume":"9 4","pages":"046101"},"PeriodicalIF":4.1,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12471802/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145187203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Credibility assessment of patient-specific modeling in transcatheter aortic valve implantation-Part 2: Uncertainty quantification and sensitivity analysis. 经导管主动脉瓣置入术中患者特异性建模的可信度评估-第二部分：不确定性量化和敏感性分析。

IF 4.1 3区医学

APL Bioengineering Pub Date : 2025-09-25 eCollection Date: 2025-12-01 DOI: 10.1063/5.0280959

Roberta Scuoppo, Chiara Catalano, Tahir Turgut, Nils Götzen, Stefano Cannata, Giovanni Gentile, Caterina Gandolfo, Salvatore Pasta

{"title":"Credibility assessment of patient-specific modeling in transcatheter aortic valve implantation-Part 2: Uncertainty quantification and sensitivity analysis.","authors":"Roberta Scuoppo, Chiara Catalano, Tahir Turgut, Nils Götzen, Stefano Cannata, Giovanni Gentile, Caterina Gandolfo, Salvatore Pasta","doi":"10.1063/5.0280959","DOIUrl":"10.1063/5.0280959","url":null,"abstract":"Transcatheter aortic valve implantation (TAVI) benefits from patient-specific computational modeling, yet model credibility remains a challenge. The ASME V&V40 standard provides a framework for assessing uncertainty and sensitivity in in silico predictions, ensuring reliability in clinical decision-making. This study evaluates uncertainty quantification (UQ) and sensitivity analysis of a patient-specific TAVI model using the ASME V&V40 standard to enhance model credibility. Four patient-specific TAVI models with 23 and 26 mm SAPIEN 3 Ultra (S3) devices were developed using finite-element simulations for deployment and fluid-structure interaction analysis for hemodynamic analysis. Uncertain parameters included anatomical features, material properties, hemodynamic conditions, and procedural variables. A surrogate model was constructed with Gaussian-process regression, and probabilistic assessment was conducted via quasi-Monte Carlo analysis. Sensitivity analysis identified key parameters influencing model outputs. The surrogate model accurately predicted device diameter (mean relative error <1%), with balloon expansion volume and stent-frame material properties being the most influential. Hemodynamic predictions exhibited greater uncertainty, with effective orifice area and pressure gradient showing deviations beyond the 5% validation threshold. This study establishes a framework for UQ in patient-specific TAVI modeling, demonstrating reliable device deployment predictions. The findings support integrating in silico models into clinical decision-making, benefiting clinicians, manufacturers, and regulatory bodies. This study is complemented by a first part dedicated to the discrete validation of the patient-specific TAVI model against clinical data.","PeriodicalId":46288,"journal":{"name":"APL Bioengineering","volume":"9 4","pages":"046102"},"PeriodicalIF":4.1,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12471805/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145187218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Machine learning-enabled detection of electrophysiological signatures in iPSC-derived models of schizophrenia and bipolar disorder. ipsc衍生的精神分裂症和双相情感障碍模型中电生理特征的机器学习检测。

IF 4.1 3区医学

APL Bioengineering Pub Date : 2025-09-22 eCollection Date: 2025-09-01 DOI: 10.1063/5.0250559

Kai Cheng, Autumn Williams, Anannya Kshirsagar, Sai Kulkarni, Rakesh Karmacharya, Deok-Ho Kim, Sridevi V Sarma, Annie Kathuria

{"title":"Machine learning-enabled detection of electrophysiological signatures in iPSC-derived models of schizophrenia and bipolar disorder.","authors":"Kai Cheng, Autumn Williams, Anannya Kshirsagar, Sai Kulkarni, Rakesh Karmacharya, Deok-Ho Kim, Sridevi V Sarma, Annie Kathuria","doi":"10.1063/5.0250559","DOIUrl":"10.1063/5.0250559","url":null,"abstract":"Neuropsychiatric disorders such as schizophrenia (SCZ) and bipolar disorder (BPD) remain challenging to diagnose due to the absence of objective biomarkers, with current assessments relying largely on subjective clinical evaluations. In this study, we present a computational analysis pipeline designed to identify disease-specific electrophysiological signatures from multi-electrode array (MEA) recordings of patient-derived cerebral organoids (COs) and two-dimensional cortical interneuron cultures (2DNs). Using a Support Vector Machine classifier optimized for high-dimensional data, we achieved 95.8% classification accuracy in distinguishing SCZ from control samples in 2DNs under both baseline and post-electrical-stimulation (PES) conditions with the extracted electrophysiological signatures. In COs, classification accuracy improved from 83.3% at baseline to 91.6% following PES, enabling robust separation of control, SCZ, and BPD cohorts. Key discriminative features included channel-specific measures of network activity, with PES significantly enhancing classification performance, particularly for BPD. These results underscore the potential of MEA-based functional phenotyping, coupled with machine learning, to uncover reliable, stimulation-sensitive electrophysiological biomarkers, offering a path toward more objective diagnosis and personalized treatment strategies for neuropsychiatric disorders.","PeriodicalId":46288,"journal":{"name":"APL Bioengineering","volume":"9 3","pages":"036118"},"PeriodicalIF":4.1,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12456967/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145139023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comprehensive review of graphene-based biosensors: Fabrication, applications, characterization and future perspectives-A review. 基于石墨烯的生物传感器：制造、应用、表征和未来展望综述。

IF 4.1 3区医学

APL Bioengineering Pub Date : 2025-09-17 eCollection Date: 2025-09-01 DOI: 10.1063/5.0266596

Yao-Tung Wang, Arvind Mukundan, Riya Karmakar, Tsung-Hsien Chen, Hardik Dhiman, Fan-Min Lin, Hsiang-Chen Wang

{"title":"A comprehensive review of graphene-based biosensors: Fabrication, applications, characterization and future perspectives-A review.","authors":"Yao-Tung Wang, Arvind Mukundan, Riya Karmakar, Tsung-Hsien Chen, Hardik Dhiman, Fan-Min Lin, Hsiang-Chen Wang","doi":"10.1063/5.0266596","DOIUrl":"10.1063/5.0266596","url":null,"abstract":"Graphene is a 2D material that has emerged as a versatile and advanced material for biosensing technology due to its large surface area, high conductivity, and biocompatibility. These properties make it well-suited for label-free detection of biomarkers with high sensitivity and accuracy, which is crucial for early diagnosis of various diseases, environmental monitoring, and food safety. This review highlights recent progress in graphene-based biosensor technologies, emphasizing key fabrication methods such as mechanical exfoliation, liquid phase exfoliation, chemical vapor deposition, electrochemical exfoliation, and microwave-assisted exfoliation, which are highly effective and suitable for generating graphene at an industry level. Furthermore, the study highlights characterization techniques such as Raman spectroscopy, optical spectroscopy, scanning electron microscope, transmission electron microscopy, and atomic force microscopy, which ensure quality and functionality of the graphene in biosensing applications. While hurdles like enhancing conductivity and achieving large-scale production remain, graphene-based biosensors offer a transformative approach to delivering precise and consistent results across various industries, paving the way for innovative solutions in diagnostics and monitoring systems.","PeriodicalId":46288,"journal":{"name":"APL Bioengineering","volume":"9 3","pages":"031504"},"PeriodicalIF":4.1,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12448920/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145114547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The glioblastoma biomechanical landscape: A systematic review of magnetic resonance elastography (MRE) of brain tumors and healthy brain. 胶质母细胞瘤的生物力学景观：脑肿瘤和健康大脑的磁共振弹性成像（MRE）的系统综述。

IF 4.1 3区医学

APL Bioengineering Pub Date : 2025-09-12 eCollection Date: 2025-09-01 DOI: 10.1063/5.0277950

Thuvarahan Jegathees, Lauriane Jugé, Eric Hau, Lynne E Bilston, Geraldine M O'Neill

{"title":"The glioblastoma biomechanical landscape: A systematic review of magnetic resonance elastography (MRE) of brain tumors and healthy brain.","authors":"Thuvarahan Jegathees, Lauriane Jugé, Eric Hau, Lynne E Bilston, Geraldine M O'Neill","doi":"10.1063/5.0277950","DOIUrl":"10.1063/5.0277950","url":null,"abstract":"Diagnosis of a glioblastoma (GBM) brain tumor is associated with very poor prognosis. Currently, few preclinical models used to identify new therapies address the soft brain tissue environment and GBM mechanoresponses, which are implicated in disease progression. Understanding the GBM biomechanical landscape is critical to deriving improved preclinical models and magnetic resonance elastography (MRE) holds promise to address this gap. Due to technical and feasibility issues for MRE of patient tumors at scale, most studies only report on small cohorts of patients, thus limiting the conclusions that may be drawn from individual studies. To thus gain a better overview, we have undertaken a systematic review and meta-analysis of the reported tissue viscoelastic property values from studies of both healthy brain and brain tumors, with a particular focus on delineating measurements relative to MRE transducer vibration frequency. Based on these analyses, healthy white matter consistently appears stiffer than gray matter. Further, analyses of pooled healthy brain tissue measurements vs human GBM suggested that, overall, the GBM has the same stiffness as the surrounding healthy tissue. This contrasted with mouse models of GBM, where the tumors appear softer than brain tissue. The limited number of studies of human GBM in situ is a caveat to these conclusions and MRE analyses of larger GBM patient cohorts are urgently needed. Meanwhile, the information from this analysis can be used to guide engineering of improved preclinical models with features that recapitulate the in vivo brain tissue environment.","PeriodicalId":46288,"journal":{"name":"APL Bioengineering","volume":"9 3","pages":"031503"},"PeriodicalIF":4.1,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12433313/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145065998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3D bioprinted human iPSC-derived neural progenitor cells as a novel platform for studying neurogenic niche. 生物3D打印人类ipsc衍生的神经祖细胞作为研究神经源性生态位的新平台。

IF 4.1 3区医学

APL Bioengineering Pub Date : 2025-09-09 eCollection Date: 2025-09-01 DOI: 10.1063/5.0276704

Lucas Simões Machado, Paula Scanavez Ferreira, Marina Rodrigues Pires, Larissa Valdemarin Bim, Natália Heloísa de Oliveira, Geisa Rodrigues Salles, Natalia Dall'Agnol Ferreira, Elisa Marozzi Cruz, Marimelia Aparecida Porcionatto

{"title":"3D bioprinted human iPSC-derived neural progenitor cells as a novel platform for studying neurogenic niche.","authors":"Lucas Simões Machado, Paula Scanavez Ferreira, Marina Rodrigues Pires, Larissa Valdemarin Bim, Natália Heloísa de Oliveira, Geisa Rodrigues Salles, Natalia Dall'Agnol Ferreira, Elisa Marozzi Cruz, Marimelia Aparecida Porcionatto","doi":"10.1063/5.0276704","DOIUrl":"10.1063/5.0276704","url":null,"abstract":"Animal models, especially rodents, used to study neurodevelopment have significantly advanced our comprehension of cellular and molecular mechanisms. Nevertheless, differences in species-specific structures, gestation periods, and interneuronal connections limit animal models' ability to represent human neurodevelopment accurately. The unique characteristics of primate neural progenitor cells (NPCs) enable cortex expansion with gyrus formation, which does not occur in lissencephalic animals, like rodents. Therefore, there is a need for novel in vitro models using human cells that recapitulate the complexity of human brain development. Along with organoids, 3D bioprinting offers a platform for creating more complex in vitro models. We developed, extensively characterized, and successfully used a Geltrex™/GelMA hydrogel blend to bioprint human induced pluripotent stem cells-derived NPCs (hNPCs). We show that 3D bioprinted hNPCs can self-organize, revealing key features of a neurogenic niche, including proliferation, differentiation, and migration, remaining viable for over 110 days. Within the first 20 days, bioprinted constructs showed the formation of positive cell clusters for the neurogenic niche cell markers FABP7, NESTIN, and GFAP. Clusters were interconnected by process bundles supporting cell migration. The cells proliferated within the clusters, and over time, NPCs originated TUBB3+ neurons with long axonal tracts, prominent around the clusters. We propose this as a 4D model to study neurogenic niches' key cellular and molecular features in a 3D bioprinted scaffold, adding time as the fourth dimension. Neuronal maturation in this dynamic model recapitulates key neurogenic niche properties, making it suitable for neurodevelopmental disease modeling and drug screening.","PeriodicalId":46288,"journal":{"name":"APL Bioengineering","volume":"9 3","pages":"036116"},"PeriodicalIF":4.1,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12422755/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145041810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0