APL Bioengineering最新文献_第2页

A wireless and handheld optical palpation imaging probe for use in breast-conserving surgery. 一种用于保乳手术的无线手持式光学触诊成像探头。

IF 4.9 3区医学

APL Bioengineering Pub Date : 2026-05-05 eCollection Date: 2026-06-01 DOI: 10.1063/5.0323681

Rhys Jones, Renate Zilkens, Ankit Bharakhda, Mireille Hardie, Christobel M Saunders, Qi Fang, Brendan F Kennedy

{"title":"A wireless and handheld optical palpation imaging probe for use in breast-conserving surgery.","authors":"Rhys Jones, Renate Zilkens, Ankit Bharakhda, Mireille Hardie, Christobel M Saunders, Qi Fang, Brendan F Kennedy","doi":"10.1063/5.0323681","DOIUrl":"10.1063/5.0323681","url":null,"abstract":"We present a wireless and handheld optical elastography probe aimed toward improving intraoperative discrimination between malignant and benign tissue in breast-conserving surgery. If successful, this probe can contribute to reducing close or positive margins and, therefore, subsequent re-excisions. The probe visualizes mechanical contrast between tumor and surrounding benign tissue in excised human breast specimens using stereoscopic optical palpation, in which the deformation of a compliant silicone layer is measured by two parallel cameras to infer surface stress, where variations in stress correspond to differences in the mechanical properties of the underlying tissue. Wireless operation is achieved using a Wi-Fi transceiver to transmit images at 15 fps to a laptop for processing. To enhance image quality, we incorporate computational optical palpation, which utilizes finite element analysis to provide a more accurate mechanical model of the deformation of the compliant layer. This approach yields a twofold improvement in spatial resolution, from 1034 to 512 μm, and a 55% increase in stress contrast in a structured silicone phantom. In a preliminary study on four excised human breast tissue samples, we demonstrate that the probe can identify tumor and distinguish between benign tissue types, including adipose tissue, stroma, and potentially ducts.","PeriodicalId":46288,"journal":{"name":"APL Bioengineering","volume":"10 2","pages":"026104"},"PeriodicalIF":4.9,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13143436/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147844114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Force-sensing mobile microrobotic grippers for gentle and precise bioassembly of cell spheroids. 用于细胞球体的温和和精确生物组装的力传感移动微型机器人夹具。

IF 4.9 3区医学

APL Bioengineering Pub Date : 2026-04-28 eCollection Date: 2026-06-01 DOI: 10.1063/5.0304932

Aaron C Davis, Madison O'Brien, Iris Gong, Luis Solorio, David J Cappelleri

引用次数: 0

Erratum: "Credibility assessment of patient-specific modeling in transcatheter aortic valve implantation. I. A population-based validation of patient-specific modeling" [APL Bioeng. 9, 046101 (2025)]. 勘误：经导管主动脉瓣植入术中患者特异性建模的可信度评估。1 .基于人群的患者特异性模型验证[j].中华生物医学工程杂志，9,046101（2025）。

IF 4.9 3区医学

APL Bioengineering Pub Date : 2026-04-22 eCollection Date: 2026-06-01 DOI: 10.1063/5.0335660

Chiara Catalano, Roberta Scuoppo, Tahir Turgut, Vincent Bouwman, Nils Götzen, Stefano Cannata, Giovanni Gentile, Caterina Gandolfo, Salvatore Pasta

引用次数: 0

Erratum: "Credibility assessment of patient-specific modeling in transcatheter aortic valve implantation-Part 2: Uncertainty quantification and sensitivity analysis" [APL Bioeng. 9, 046102 (2025)]. 勘误：“经导管主动脉瓣植入中患者特异性建模的可信度评估-第2部分：不确定性量化和敏感性分析”[APL生物工程，9,046102(2025)]。

IF 4.9 3区医学

APL Bioengineering Pub Date : 2026-04-22 eCollection Date: 2026-06-01 DOI: 10.1063/5.0335662

Roberta Scuoppo, Chiara Catalano, Tahir Turgut, Nils Götzen, Stefano Cannata, Giovanni Gentile, Caterina Gandolfo, Salvatore Pasta

引用次数: 0

Research progress in flap temperature monitoring technologies and applications after transplantation. 皮瓣移植后温度监测技术及应用研究进展。

IF 4.9 3区医学

APL Bioengineering Pub Date : 2026-04-09 eCollection Date: 2026-06-01 DOI: 10.1063/5.0314573

Tingting Hu, Tao Yan, Junjie Chen, Qin Huang, Miao Cui, Yanting Han, Ka Li

{"title":"Research progress in flap temperature monitoring technologies and applications after transplantation.","authors":"Tingting Hu, Tao Yan, Junjie Chen, Qin Huang, Miao Cui, Yanting Han, Ka Li","doi":"10.1063/5.0314573","DOIUrl":"10.1063/5.0314573","url":null,"abstract":"As a primary method for tissue repair and functional reconstruction, flap transplantation has achieved a clinical success rate of over 90%. However, the postoperative incidence of vascular crisis remains as high as 10%-30%, making it a leading cause of reoperation and disability. Flap temperature is a key indicator closely correlated with microcirculatory status, making its monitoring essential for the early detection of complications. This paper provides a systematic review of the physiological mechanisms underlying postoperative flap temperature, the characteristic temperature changes associated with venous and arterial crises, and the latest advancements in monitoring technologies. It comprehensively analyzes the principles, advantages, and limitations of various methods, including manual palpation, contact thermometry, infrared thermography, fiber optic sensing, and microwave thermometry. Furthermore, the review explores the application of intelligent technologies such as wearable sensors, artificial intelligence-driven predictive systems, implantable flexible devices, and multimodal fusion monitoring. Current challenges, including poor real-time performance, low precision, and a lack of standardization, are highlighted. Future development is directed toward precision, intelligence, and integration, with an emphasis on multidisciplinary collaboration to create more accurate, convenient, and intelligent monitoring systems. These advancements aim to achieve precise early warning and timely intervention, ultimately improving flap survival rates and patient outcomes.","PeriodicalId":46288,"journal":{"name":"APL Bioengineering","volume":"10 2","pages":"021503"},"PeriodicalIF":4.9,"publicationDate":"2026-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13068449/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147677603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mapping the miRNA landscape of primitive macrophage extracellular vesicles highlights their pro-vasculogenic effects in engineered human cardiac tissue. 绘制原始巨噬细胞细胞外囊泡的miRNA景观，突出了它们在工程人类心脏组织中的促血管生成作用。

IF 4.9 3区医学

APL Bioengineering Pub Date : 2026-04-07 eCollection Date: 2026-06-01 DOI: 10.1063/5.0313731

Karl T Wagner, Shira Landau, Gregory M Kent, David F Bodenstein, Milica Radisic

{"title":"Mapping the miRNA landscape of primitive macrophage extracellular vesicles highlights their pro-vasculogenic effects in engineered human cardiac tissue.","authors":"Karl T Wagner, Shira Landau, Gregory M Kent, David F Bodenstein, Milica Radisic","doi":"10.1063/5.0313731","DOIUrl":"10.1063/5.0313731","url":null,"abstract":"Resident cardiac macrophages, derived from primitive yolk sac precursors during embryogenesis, have increasingly been recognized for their distinct phenotype and functions in regulating homeostasis of the human heart. However, the profile of their extracellular vesicles (EVs) in cardiac signaling and regulation remains uncharted. Here, we employ differentiation of human pluripotent stem cell-derived primitive macrophages (Mac), harvesting their secreted EVs and performing in-depth characterization of associated microRNAs (miRNAs). Primitive macrophages secreted nanoscale EVs that expressed canonical EV markers, and miRNA sequencing highlighted a diverse and unique profile of miRNAs when compared to EVs sourced from other principal cardiac cell lineages and published data from monocyte-derived cells. In particular, we noted the abundance and enrichment of vascular-modulatory let-7 miRNAs and miR-126-3p. Functional screening of Mac-EVs in a 3D model of in vitro cardiac vasculogenesis confirmed enhanced early endothelial cell organization and branching. Establishing a reference for the human Mac-EV miRNome enables further hypothesis-driven mechanistic tests of Mac-EV miRNAs in mediating cardiac physiology and disease, opening the door to identification of therapeutic targets and modalities for cardiac repair.","PeriodicalId":46288,"journal":{"name":"APL Bioengineering","volume":"10 2","pages":"026102"},"PeriodicalIF":4.9,"publicationDate":"2026-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13065346/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147677597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research advances in intelligent microenvironment-responsive hydrogels for myocardial infarction therapy. 智能微环境响应型水凝胶治疗心肌梗死的研究进展。

IF 4.9 3区医学

APL Bioengineering Pub Date : 2026-04-02 eCollection Date: 2026-06-01 DOI: 10.1063/5.0312953

Jian Li, Jinglin Gao, Kaiyi Zhu, Yuping Gao

{"title":"Research advances in intelligent microenvironment-responsive hydrogels for myocardial infarction therapy.","authors":"Jian Li, Jinglin Gao, Kaiyi Zhu, Yuping Gao","doi":"10.1063/5.0312953","DOIUrl":"10.1063/5.0312953","url":null,"abstract":"Myocardial infarction (MI) poses a severe threat to human life and health. During acute MI, persistent myocardial ischemia and hypoxia induce pathological alterations in the microenvironment. Traditional therapeutic approaches exhibit limited capacity for targeted modulation of this infarcted microenvironment. Consequently, developing therapeutic strategies capable of precisely responding to the pathological microenvironment holds significant importance. Hydrogels, as a class of polymeric biomaterials with excellent biocompatibility, can be engineered into intelligent responsive hydrogels by incorporating environmentally responsive functional groups or constructing intelligent network architectures. These hydrogels are designed to sense and respond to key features of the MI pathological microenvironment, such as temperature, pH, reactive oxygen species, and enzyme concentrations. This review systematically summarizes the design strategies and research advances in intelligent responsive hydrogels for MI therapy over recent years, focusing on their distinct functional capabilities: alleviating oxidative damage, suppressing excessive inflammatory responses, enabling precise drug delivery, and modulating immune activity. Although current research predominantly remains at the preclinical stage and faces numerous challenges, the convergence of materials science and biomedical engineering positions smart responsive hydrogels as promising candidates to deliver innovative solutions for the precise treatment of MI.","PeriodicalId":46288,"journal":{"name":"APL Bioengineering","volume":"10 2","pages":"021501"},"PeriodicalIF":4.9,"publicationDate":"2026-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13048829/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147624069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cardiac mechanotransduction from development to disease. 心脏机械转导从发育到疾病。

IF 4.9 3区医学

APL Bioengineering Pub Date : 2026-04-01 eCollection Date: 2026-06-01 DOI: 10.1063/5.0320788

Na Yeon Kim, Hyojung Jo, Chloe Becker, Isaiah Soicher, Yeqing Ni, Sangkyun Cho, Hyeonyu Kim

{"title":"Cardiac mechanotransduction from development to disease.","authors":"Na Yeon Kim, Hyojung Jo, Chloe Becker, Isaiah Soicher, Yeqing Ni, Sangkyun Cho, Hyeonyu Kim","doi":"10.1063/5.0320788","DOIUrl":"10.1063/5.0320788","url":null,"abstract":"Mechanical cues control key aspects of cardiac structure formation and function from heart development through adult life. Because the heart is a pump, forces from muscle contraction and blood flow generate normal and shear stresses that, together with matrix stiffness, regulate cell fate, growth, and homeostasis through mechanotransduction. This review describes how mechanosensors in cardiomyocytes, endothelial cells, and fibroblasts, including integrins and stretch-activated ion channels, couple mechanical stimuli to their cellular responses. We outline pathways that translate force into key phenotypes relevant to morphogenesis, homeostasis, and disease progression, with emphasis on RhoA/ROCK, calcium, and Yes-associated protein (YAP) signaling. We also explain how elevated mechanical load driven by hypertension activates hypertrophic and fibrotic remodeling of cardiac chambers, particularly through transforming growth factor-β, integrins, YAP, and calcineurin signaling. Finally, we highlight emerging roles for mechanosensitive microRNAs in coordinating proliferation, metabolism, electrophysiology, and extracellular matrix dynamics in the heart. Since most, if not all, of these pathways are interconnected, a comprehensive understanding will require high-resolution maps of cardiac mechanical environments and clear links between defined stimuli and cell-type-specific responses. These insights will advance fundamental understanding and guide the development of more effective therapeutic strategies.","PeriodicalId":46288,"journal":{"name":"APL Bioengineering","volume":"10 2","pages":"021502"},"PeriodicalIF":4.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13046396/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147624107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selective capture and digital counting of intact HIV pseudovirus using designer DNA nets, tethered motion, and photonic resonator interferometric scattering microscopy. 利用设计DNA网、系绳运动和光子谐振器干涉散射显微镜对完整的HIV假病毒进行选择性捕获和数字计数。

IF 4.9 3区医学

APL Bioengineering Pub Date : 2026-04-01 eCollection Date: 2026-06-01 DOI: 10.1063/5.0289982

Joseph Tibbs, Saurabh Umrao, Tingjie Song, Varada Anirudhan, Skye Shepherd, Katy Wolhaupter, Mengxi Zheng, Sydney Wiggins, Lijun Rong, Colin L Hisey, Utkan Demirci, Xing Wang, Brian T Cunningham

{"title":"Selective capture and digital counting of intact HIV pseudovirus using designer DNA nets, tethered motion, and photonic resonator interferometric scattering microscopy.","authors":"Joseph Tibbs, Saurabh Umrao, Tingjie Song, Varada Anirudhan, Skye Shepherd, Katy Wolhaupter, Mengxi Zheng, Sydney Wiggins, Lijun Rong, Colin L Hisey, Utkan Demirci, Xing Wang, Brian T Cunningham","doi":"10.1063/5.0289982","DOIUrl":"10.1063/5.0289982","url":null,"abstract":"Rapid and quantitatively accurate detection of HIV (human immunodeficiency virus) viral load using a simple workflow, automated instrumentation, and real-time data processing with easily interpretable output is required for an approach to become practical for point-of-care environments. We recently demonstrated a form of interferometric scattering microscopy called Photonic Resonator Interferometric Scattering Microscopy (PRISM) that amplifies the contrast of surface-attached nano-objects via a photonic crystal (PC) surface. Recently, our team also developed net-shaped DNA nanostructures called \"Designer DNA Nets\" (DDN) that organize multivalent aptamer arrays to precisely match the pattern of proteins on the outer surface of intact virions to provide high-affinity and selective binding. In this work, we demonstrate the combination of DDNs and PRISM for detection of HIV by digital counting of captured viruses. We compare multivalent DDN-based viral capture to monomeric aptamer and nanobody capture, in which the captured virions are tethered to the PC surface by a DNA linker. We observe that tethered virions are not fully stationary and that their localized dynamic movement provides a route for label-free digital-resolution detection with a signal-to-noise ratio of 50, while disregarding the presence of image features not related to specific virus capture. We obtain a detection limit of 104 virions/ml with a single-step, room temperature 30-min assay and excellent selectivity for non-detection of a nonspecific virus and the presence of a high concentration of extracellular vesicles. This study highlights PRISM's utility as a means for versatile detection of immobilized particles as part of an assay for affinity molecule evaluation.","PeriodicalId":46288,"journal":{"name":"APL Bioengineering","volume":"10 2","pages":"026101"},"PeriodicalIF":4.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13046394/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147624071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ChatGPT in precision medicine. 精准医疗中的ChatGPT。

IF 4.9 3区医学

APL Bioengineering Pub Date : 2026-03-17 eCollection Date: 2026-03-01 DOI: 10.1063/5.0299327

Chengliang Yin, Xiaochun Sun, Anlin Dai, Xin-Yao Ye, Yi Lu, Wanling Wang, Yuanyuan Chen, Hong Jiang, Juan Yu, Siomui Chong, Mingming Jiang, Jiayu Xu, Bing Yang, Rajeswari Chappa, Santosh Chokkakula, Kunlun He

{"title":"ChatGPT in precision medicine.","authors":"Chengliang Yin, Xiaochun Sun, Anlin Dai, Xin-Yao Ye, Yi Lu, Wanling Wang, Yuanyuan Chen, Hong Jiang, Juan Yu, Siomui Chong, Mingming Jiang, Jiayu Xu, Bing Yang, Rajeswari Chappa, Santosh Chokkakula, Kunlun He","doi":"10.1063/5.0299327","DOIUrl":"10.1063/5.0299327","url":null,"abstract":"The integration of artificial intelligence, notably the generative language model ChatGPT, into precision medicine precedes a paradigm shift in healthcare. This Review explores the capabilities of ChatGPT to process large data sets, understand contextual details, and generate human-like responses, making it a transformative tool for various applications in precision medicine. By analyzing the genetic sequences, identifying biomarkers, and predicting disease risks, ChatGPT facilitates earlier diagnosis and intervention. Moreover, its natural language processing capabilities enhance patient communication and engagement by elucidating complex medical concepts. This Review further examines ChatGPT's role in streamlining research, augmenting clinical trial recruitment, and supporting healthcare professionals through access to updated information and skill assessment. While acknowledging the complexities and potential concerns regarding reliance on publicly sourced data, this Review highlights ChatGPT's substantial capacity to optimize patient prognoses and enhance the operational efficacy of the healthcare delivery system.","PeriodicalId":46288,"journal":{"name":"APL Bioengineering","volume":"10 1","pages":"011502"},"PeriodicalIF":4.9,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12999217/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147487884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0