APL Bioengineering最新文献_第2页

A high throughput cell stretch device for investigating mechanobiology in vitro. 用于研究体外机械生物学的高通量细胞拉伸装置。

IF 6.6 3区医学

APL Bioengineering Pub Date : 2024-06-26 eCollection Date: 2024-06-01 DOI: 10.1063/5.0206852

Stephen J P Pratt, Christopher M Plunkett, Guray Kuzu, Ton Trinh, Joshua Barbara, Paula Choconta, Doug Quackenbush, Truc Huynh, Anders Smith, S Whitney Barnes, Joel New, James Pierce, John R Walker, James Mainquist, Frederick J King, Jimmy Elliott, Scott Hammack, Rebekah S Decker

{"title":"A high throughput cell stretch device for investigating mechanobiology in vitro.","authors":"Stephen J P Pratt, Christopher M Plunkett, Guray Kuzu, Ton Trinh, Joshua Barbara, Paula Choconta, Doug Quackenbush, Truc Huynh, Anders Smith, S Whitney Barnes, Joel New, James Pierce, John R Walker, James Mainquist, Frederick J King, Jimmy Elliott, Scott Hammack, Rebekah S Decker","doi":"10.1063/5.0206852","DOIUrl":"https://doi.org/10.1063/5.0206852","url":null,"abstract":"Mechanobiology is a rapidly advancing field, with growing evidence that mechanical signaling plays key roles in health and disease. To accelerate mechanobiology-based drug discovery, novel in vitro systems are needed that enable mechanical perturbation of cells in a format amenable to high throughput screening. Here, both a mechanical stretch device and 192-well silicone flexible linear stretch plate were designed and fabricated to meet high throughput technology needs for cell stretch-based applications. To demonstrate the utility of the stretch plate in automation and screening, cell dispensing, liquid handling, high content imaging, and high throughput sequencing platforms were employed. Using this system, an assay was developed as a biological validation and proof-of-concept readout for screening. A mechano-transcriptional stretch response was characterized using focused gene expression profiling measured by RNA-mediated oligonucleotide Annealing, Selection, and Ligation with Next-Gen sequencing. Using articular chondrocytes, a gene expression signature containing stretch responsive genes relevant to cartilage homeostasis and disease was identified. The possibility for integration of other stretch sensitive cell types (e.g., cardiovascular, airway, bladder, gut, and musculoskeletal), in combination with alternative phenotypic readouts (e.g., protein expression, proliferation, or spatial alignment), broadens the scope of high throughput stretch and allows for wider adoption by the research community. This high throughput mechanical stress device fills an unmet need in phenotypic screening technology to support drug discovery in mechanobiology-based disease areas.","PeriodicalId":46288,"journal":{"name":"APL Bioengineering","volume":null,"pages":null},"PeriodicalIF":6.6,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11210978/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141471495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adsorption of monoclonal antibody fragments at the water-oil interface: A coarse-grained molecular dynamics study. 单克隆抗体片段在水油界面的吸附：粗粒度分子动力学研究。

IF 6.6 3区医学

APL Bioengineering Pub Date : 2024-06-25 eCollection Date: 2024-06-01 DOI: 10.1063/5.0207959

Suman Saurabh, Li Lei, Zongyi Li, John M Seddon, Jian R Lu, Cavan Kalonia, Fernando Bresme

{"title":"Adsorption of monoclonal antibody fragments at the water-oil interface: A coarse-grained molecular dynamics study.","authors":"Suman Saurabh, Li Lei, Zongyi Li, John M Seddon, Jian R Lu, Cavan Kalonia, Fernando Bresme","doi":"10.1063/5.0207959","DOIUrl":"10.1063/5.0207959","url":null,"abstract":"Monoclonal antibodies (mAbs) can undergo structural changes due to interaction with oil-water interfaces during storage. Such changes can lead to aggregation, resulting in a loss of therapeutic efficacy. Therefore, understanding the microscopic mechanism controlling mAb adsorption is crucial to developing strategies that can minimize the impact of interfaces on the therapeutic properties of mAbs. In this study, we used MARTINI coarse-grained molecular dynamics simulations to investigate the adsorption of the Fab and Fc domains of the monoclonal antibody COE3 at the oil-water interface. Our aim was to determine the regions on the protein surface that drive mAb adsorption. We also investigate the role of protein concentration on protein orientation and protrusion to the oil phase. While our structural analyses compare favorably with recent neutron reflectivity measurements, we observe some differences. Unlike the monolayer at the interface predicted by neutron reflectivity experiments, our simulations indicate the presence of a secondary diffused layer near the interface. We also find that under certain conditions, protein-oil interaction can lead to a considerable distortion in the protein structure, resulting in enhanced adsorption behavior.","PeriodicalId":46288,"journal":{"name":"APL Bioengineering","volume":null,"pages":null},"PeriodicalIF":6.6,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11211994/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141471496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamic reporters for probing real-time activation of human fibroblasts from single cells to populations. 用于探测人类成纤维细胞从单细胞到群体的实时活化的动态报告器。

IF 6.6 3区医学

APL Bioengineering Pub Date : 2024-06-24 eCollection Date: 2024-06-01 DOI: 10.1063/5.0166152

Samantha E Cassel, Breanna M Huntington, Wilfred Chen, Pedro Lei, Stelios T Andreadis, April M Kloxin

{"title":"Dynamic reporters for probing real-time activation of human fibroblasts from single cells to populations.","authors":"Samantha E Cassel, Breanna M Huntington, Wilfred Chen, Pedro Lei, Stelios T Andreadis, April M Kloxin","doi":"10.1063/5.0166152","DOIUrl":"10.1063/5.0166152","url":null,"abstract":"Activation of fibroblasts is pivotal for wound healing; however, persistent activation leads to maladaptive processes and is a hallmark of fibrosis, where disease mechanisms are only partially understood. Human in vitro model systems complement in vivo animal models for both hypothesis testing and drug evaluation to improve the identification of therapeutics relevant to human disease. Despite advances, a challenge remains in understanding the dynamics of human fibroblast responses to complex microenvironment stimuli, motivating the need for more advanced tools to investigate fibrotic mechanisms. This work established approaches for assessing the temporal dynamics of these responses using genetically encoded fluorescent reporters of alpha smooth muscle actin expression, an indicator of fibroblast activation. Specifically, we created a toolset of human lung fibroblast reporter cell lines from different origins (male, female; healthy, idiopathic pulmonary fibrosis) and used three different versions of the reporter with the fluorescent protein modified to exhibit different temporal stabilities, providing temporal resolution of protein expression processes over a range of timescales. Using this toolset, we demonstrated that reporters provide insight into population shifts in response to both mechanical and biochemical cues that are not detectable by traditional end point assessments with differential responses based on cell origin. Furthermore, individual cells can also be tracked over time, with opportunities for comparison to complementary end point measurements. The establishment of this reporter toolset enables dynamic cell investigations that can be translated into more complex synthetic culture environments for elucidating disease mechanisms and evaluating therapeutics for lung fibrosis and other complex biological processes more broadly.","PeriodicalId":46288,"journal":{"name":"APL Bioengineering","volume":null,"pages":null},"PeriodicalIF":6.6,"publicationDate":"2024-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11209894/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141471497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multicompartment duct platform to study epithelial-endothelial crosstalk associated with lung adenocarcinoma. 研究与肺腺癌相关的上皮-内皮串联的多室导管平台。

IF 6.6 3区医学

APL Bioengineering Pub Date : 2024-06-17 eCollection Date: 2024-06-01 DOI: 10.1063/5.0207228

Keith A Gagnon, Jessie Huang, Olivia T Hix, Veronica W Hui, Anne Hinds, Esther Bullitt, Jeroen Eyckmans, Darrell N Kotton, Christopher S Chen

{"title":"Multicompartment duct platform to study epithelial-endothelial crosstalk associated with lung adenocarcinoma.","authors":"Keith A Gagnon, Jessie Huang, Olivia T Hix, Veronica W Hui, Anne Hinds, Esther Bullitt, Jeroen Eyckmans, Darrell N Kotton, Christopher S Chen","doi":"10.1063/5.0207228","DOIUrl":"10.1063/5.0207228","url":null,"abstract":"Previous lung-on-chip devices have facilitated significant advances in our understanding of lung biology and pathology. Here, we describe a novel lung-on-a-chip model in which human induced pluripotent stem cell-derived alveolar epithelial type II cells (iAT2s) form polarized duct-like lumens alongside engineered perfused vessels lined with human umbilical vein endothelium, all within a 3D, physiologically relevant microenvironment. Using this model, we investigated the morphologic and signaling consequences of the KRASG12D mutation, a commonly identified oncogene in human lung adenocarcinoma (LUAD). We show that expression of the mutant KRASG12D isoform in iAT2s leads to a hyperproliferative response and morphologic dysregulation in the epithelial monolayer. Interestingly, the mutant epithelia also drive an angiogenic response in the adjacent vasculature that is mediated by enhanced secretion of the pro-angiogenic factor soluble uPAR. These results demonstrate the functionality of a multi-cellular in vitro platform capable of modeling mutation-specific behavioral and signaling changes associated with lung adenocarcinoma.","PeriodicalId":46288,"journal":{"name":"APL Bioengineering","volume":null,"pages":null},"PeriodicalIF":6.6,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11191334/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141443517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Parallel on-chip micropipettes enabling quantitative multiplexed characterization of vesicle mechanics and cell aggregates rheology. 可对囊泡力学和细胞聚集流变学进行定量多路复用表征的并行芯片上微量移液器。

IF 6 3区医学

APL Bioengineering Pub Date : 2024-06-12 eCollection Date: 2024-06-01 DOI: 10.1063/5.0193333

Sylvain Landiech, Marianne Elias, Pierre Lapèze, Hajar Ajiyel, Marine Plancke, Blanca González-Bermúdez, Adrian Laborde, Fabien Mesnilgrente, David Bourrier, Debora Berti, Costanza Montis, Laurent Mazenq, Jérémy Baldo, Clément Roux, Morgan Delarue, Pierre Joseph

{"title":"Parallel on-chip micropipettes enabling quantitative multiplexed characterization of vesicle mechanics and cell aggregates rheology.","authors":"Sylvain Landiech, Marianne Elias, Pierre Lapèze, Hajar Ajiyel, Marine Plancke, Blanca González-Bermúdez, Adrian Laborde, Fabien Mesnilgrente, David Bourrier, Debora Berti, Costanza Montis, Laurent Mazenq, Jérémy Baldo, Clément Roux, Morgan Delarue, Pierre Joseph","doi":"10.1063/5.0193333","DOIUrl":"10.1063/5.0193333","url":null,"abstract":"Micropipette aspiration (MPA) is one of the gold standards for quantifying biological samples' mechanical properties, which are crucial from the cell membrane scale to the multicellular tissue. However, relying on the manipulation of individual home-made glass pipettes, MPA suffers from low throughput and no automation. Here, we introduce the sliding insert micropipette aspiration method, which permits parallelization and automation, thanks to the insertion of tubular pipettes, obtained by photolithography, within microfluidic channels. We show its application both at the lipid bilayer level, by probing vesicles to measure membrane bending and stretching moduli, and at the tissue level by quantifying the viscoelasticity of 3D cell aggregates. This approach opens the way to high-throughput, quantitative mechanical testing of many types of biological samples, from vesicles and individual cells to cell aggregates and explants, under dynamic physico-chemical stimuli.","PeriodicalId":46288,"journal":{"name":"APL Bioengineering","volume":null,"pages":null},"PeriodicalIF":6.0,"publicationDate":"2024-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11184969/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141421356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An optimized convolutional neural network architecture for lung cancer detection. 用于肺癌检测的优化卷积神经网络架构

IF 6 3区医学

APL Bioengineering Pub Date : 2024-06-11 eCollection Date: 2024-06-01 DOI: 10.1063/5.0208520

Sameena Pathan, Tanweer Ali, Sudheesh P G, Vasanth Kumar P, Divya Rao

{"title":"An optimized convolutional neural network architecture for lung cancer detection.","authors":"Sameena Pathan, Tanweer Ali, Sudheesh P G, Vasanth Kumar P, Divya Rao","doi":"10.1063/5.0208520","DOIUrl":"10.1063/5.0208520","url":null,"abstract":"Lung cancer, the treacherous malignancy affecting the respiratory system of a human body, has a devastating impact on the health and well-being of an individual. Due to the lack of automated and noninvasive diagnostic tools, healthcare professionals look forward toward biopsy as a gold standard for diagnosis. However, biopsy could be traumatizing and expensive process. Additionally, the limited availability of dataset and inaccuracy in diagnosis is a major drawback experienced by researchers. The objective of the proposed research is to develop an automated diagnostic tool for screening of lung cancer using optimized hyperparameters such that convolutional neural network (CNN) model generalizes well for universally obtained computerized tomography (CT) slices of lung pathologies. The aforementioned objective is achieved in the following ways: (i) Initially, a preprocessing methodology specific to lung CT scans is formulated to avoid the loss of information due to random image smoothing, and (ii) a sine cosine algorithm optimization algorithm (SCA) is integrated in the CNN model, to optimally select the tuning parameters of CNN. The error rate is used as an objective function, and the SCA algorithm tries to minimize. The proposed method successfully achieved an average classification accuracy of 99% in classification of lung scans in normal, benign, and malignant classes. Further, the generalization ability of the proposed model is tested on unseen dataset, thereby achieving promising results. The quantitative results prove the efficacy of the system to be used by radiologists in a clinical scenario.","PeriodicalId":46288,"journal":{"name":"APL Bioengineering","volume":null,"pages":null},"PeriodicalIF":6.0,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11168751/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141311972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Collagen density regulates tip-stalk cell rearrangement during angiogenesis via cellular bioenergetics. 胶原蛋白密度通过细胞生物能调节血管生成过程中的顶茎细胞重排。

IF 6 3区医学

APL Bioengineering Pub Date : 2024-06-10 eCollection Date: 2024-06-01 DOI: 10.1063/5.0195249

Wenjun Wang, Matthew R Zanotelli, Lindsey N Sabo, Emily D Fabiano, Natalie M Goldfield, Chloe Le, Elle P Techasiriwan, Santiago Lopez, Emily D Berestesky, Cynthia A Reinhart-King

{"title":"Collagen density regulates tip-stalk cell rearrangement during angiogenesis via cellular bioenergetics.","authors":"Wenjun Wang, Matthew R Zanotelli, Lindsey N Sabo, Emily D Fabiano, Natalie M Goldfield, Chloe Le, Elle P Techasiriwan, Santiago Lopez, Emily D Berestesky, Cynthia A Reinhart-King","doi":"10.1063/5.0195249","DOIUrl":"10.1063/5.0195249","url":null,"abstract":"Tumor vasculature plays a crucial role in tumor progression, affecting nutrition and oxygen transportation as well as the efficiency of drug delivery. While targeting pro-angiogenic growth factors has been a significant focus for treating tumor angiogenesis, recent studies indicate that metabolism also plays a role in regulating endothelial cell behavior. Like cancer cells, tumor endothelial cells undergo metabolic changes that regulate rearrangement for tip cell position during angiogenesis. Our previous studies have shown that altered mechanical properties of the collagen matrix regulate angiogenesis and can promote a tumor vasculature phenotype. Here, we examine the effect of collagen density on endothelial cell tip-stalk cell rearrangement and cellular energetics during angiogenic sprouting. We find that increased collagen density leads to an elevated energy state and an increased rate of tip-stalk cell switching, which is correlated with the energy state of the cells. Tip cells exhibit higher glucose uptake than stalk cells, and inhibition of glucose uptake revealed that invading sprouts rely on glucose to meet elevated energy requirements for invasion in dense matrices. This work helps to elucidate the complex interplay between the mechanical microenvironment and the endothelial cell metabolic status during angiogenesis, which could have important implications for developing new anti-cancer therapies.","PeriodicalId":46288,"journal":{"name":"APL Bioengineering","volume":null,"pages":null},"PeriodicalIF":6.0,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11170328/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141318492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biophysical perspectives to understanding cancer-associated fibroblasts. 从生物物理角度了解癌症相关成纤维细胞。

IF 6 3区医学

APL Bioengineering Pub Date : 2024-06-06 eCollection Date: 2024-06-01 DOI: 10.1063/5.0199024

Somayadineshraj Devarasou, Minwoo Kang, Jennifer H Shin

{"title":"Biophysical perspectives to understanding cancer-associated fibroblasts.","authors":"Somayadineshraj Devarasou, Minwoo Kang, Jennifer H Shin","doi":"10.1063/5.0199024","DOIUrl":"10.1063/5.0199024","url":null,"abstract":"The understanding of cancer has evolved significantly, with the tumor microenvironment (TME) now recognized as a critical factor influencing the onset and progression of the disease. This broader perspective challenges the traditional view that cancer is primarily caused by mutations, instead emphasizing the dynamic interaction between different cell types and physicochemical factors within the TME. Among these factors, cancer-associated fibroblasts (CAFs) command attention for their profound influence on tumor behavior and patient prognoses. Despite their recognized importance, the biophysical and mechanical interactions of CAFs within the TME remain elusive. This review examines the distinctive physical characteristics of CAFs, their morphological attributes, and mechanical interactions within the TME. We discuss the impact of mechanotransduction on CAF function and highlight how these cells communicate mechanically with neighboring cancer cells, thereby shaping the path of tumor development and progression. By concentrating on the biomechanical regulation of CAFs, this review aims to deepen our understanding of their role in the TME and to illuminate new biomechanical-based therapeutic strategies.","PeriodicalId":46288,"journal":{"name":"APL Bioengineering","volume":null,"pages":null},"PeriodicalIF":6.0,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11161195/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141297005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fibroblast senescence-associated extracellular matrix promotes heterogeneous lung niche. 成纤维细胞衰老相关细胞外基质促进了异质性肺龛。

IF 6 3区医学

APL Bioengineering Pub Date : 2024-06-05 eCollection Date: 2024-06-01 DOI: 10.1063/5.0204393

Andrew M Howes, Nova C Dea, Deepraj Ghosh, Krishangi Krishna, Yihong Wang, Yanxi Li, Braxton Morrison, Kimani C Toussaint, Michelle R Dawson

{"title":"Fibroblast senescence-associated extracellular matrix promotes heterogeneous lung niche.","authors":"Andrew M Howes, Nova C Dea, Deepraj Ghosh, Krishangi Krishna, Yihong Wang, Yanxi Li, Braxton Morrison, Kimani C Toussaint, Michelle R Dawson","doi":"10.1063/5.0204393","DOIUrl":"10.1063/5.0204393","url":null,"abstract":"Senescent cell accumulation in the pulmonary niche is associated with heightened susceptibility to age-related disease, tissue alterations, and ultimately a decline in lung function. Our current knowledge of senescent cell-extracellular matrix (ECM) dynamics is limited, and our understanding of how senescent cells influence spatial ECM architecture changes over time is incomplete. Herein is the design of an in vitro model of senescence-associated extracellular matrix (SA-ECM) remodeling using a senescent lung fibroblast-derived matrix that captures the spatiotemporal dynamics of an evolving senescent ECM architecture. Multiphoton second-harmonic generation microscopy was utilized to examine the spatial and temporal dynamics of fibroblast SA-ECM remodeling, which revealed a biphasic process that established a disordered and heterogeneous architecture. Additionally, we observed that inhibition of transforming growth factor-β signaling during SA-ECM remodeling led to improved local collagen fiber organization. Finally, we examined patient samples diagnosed with pulmonary fibrosis to further tie our results of the in vitro model to clinical outcomes. Moreover, we observed that the senescence marker p16 is correlated with local collagen fiber disorder. By elucidating the temporal dynamics of SA-ECM remodeling, we provide further insight on the role of senescent cells and their contributions to pathological ECM remodeling.","PeriodicalId":46288,"journal":{"name":"APL Bioengineering","volume":null,"pages":null},"PeriodicalIF":6.0,"publicationDate":"2024-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11161856/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141297006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering interfacial tissues: The myotendinous junction. 界面组织工程：肌腱连接处

IF 6 3区医学

APL Bioengineering Pub Date : 2024-06-03 eCollection Date: 2024-06-01 DOI: 10.1063/5.0189221

Finn Snow, Cathal O'Connell, Peiqi Yang, Magdalena Kita, Elena Pirogova, Richard J Williams, Rob M I Kapsa, Anita Quigley

引用次数: 0