Journal of Oral Biosciences最新文献_第4页

Maxillary sinus floor augmentation using sponge- and cotton-like graft materials in a rabbit model 在兔子模型中使用海绵和棉花状移植材料进行上颌窦底隆起。

IF 2.6

Journal of Oral Biosciences Pub Date : 2025-03-01 DOI: 10.1016/j.job.2024.100586

Seigo Ohba , Rena Shido , Hideyuki Yamamoto , Masahito Hara , Yasutoshi Nishikawa , Toshihiro Kasuga , Tomohiro Yamada , Yoshinori Sumita , Tatsuo Shirota

{"title":"Maxillary sinus floor augmentation using sponge- and cotton-like graft materials in a rabbit model","authors":"Seigo Ohba , Rena Shido , Hideyuki Yamamoto , Masahito Hara , Yasutoshi Nishikawa , Toshihiro Kasuga , Tomohiro Yamada , Yoshinori Sumita , Tatsuo Shirota","doi":"10.1016/j.job.2024.100586","DOIUrl":"10.1016/j.job.2024.100586","url":null,"abstract":"<div><h3>Objectives</h3><div>Bone graft materials commonly used for maxillary sinus floor augmentation (MSFA), including hydroxyapatite (HAp) and β-tricalcium phosphate (β-TCP), are mostly granular and have poor handleability. HAp/collagen composite material (HAp/Col) and β-tricalcium phosphate (β-TCP)/poly(L-lactide-co-glycolide) (PLGA) have shown promise but their application in MSFA as bone graft materials remains unclear. Here, we investigated the bone-forming behavior of HAp/Col and β-TCP/PLGA in an MSFA rabbit model.</div></div><div><h3>Methods</h3><div>Male Japanese white rabbits were used. HAP/Col or β-TCP/PLGA was randomly applied to the MSFA model. The specimens were harvested at 4 weeks (W), 8W, 16W, and 24W after surgery, and the augmented regions were evaluated using micro-computed tomography and histological analyses.</div></div><div><h3>Results</h3><div>The graft materials were retained up to 16W in the HAp/Col group and 24W in the β-TCP/PLGA group. The augmented volume detected in the HAp/Col group at 4W was substantially reduced at subsequent time points. However, in the β-TCP/PLGA group, the volume observed at 4W was maintained up to 24W. In the HAp/Col group, the bone mineral content (BMC) at 4W was significantly lower than that at 8W (<em>p</em> = 0.03716), and this elevated BMC was significantly decreased at 16W (<em>p</em> = 0.00185) and 24W (<em>p</em> = 0.00236). In the β-TCP/PLGA group, the BMC tended to increase from 4W to 16W and then decreased.</div></div><div><h3>Conclusions</h3><div>Both HAp/Col and β-TCP/PLGA are useful for MSFA because of their ability to form new bone and good handleability. The appropriate graft material should be selected depending on the application needs while understanding the properties of the newly formed bone.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 1","pages":"Article 100586"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142591056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CCN2: A potential contributor to gingival overgrowth CCN2：牙龈过度生长的潜在因素。

IF 2.6

Journal of Oral Biosciences Pub Date : 2025-03-01 DOI: 10.1016/j.job.2024.100587

Asmaa Fadl, Andrew Leask

引用次数: 0

Exploring the mechanism of tiRNA-Val-CAC-002 in the pathogenesis of oral submucous fibrosis 探索 tiRNA-Val-CAC-002 在口腔黏膜下纤维化发病机制中的作用机制

IF 2.6

Journal of Oral Biosciences Pub Date : 2025-03-01 DOI: 10.1016/j.job.2024.100588

Liujun Zeng , Hui Peng , Leiye Sun , Huiqiao Yu , Yingfang Wu

{"title":"Exploring the mechanism of tiRNA-Val-CAC-002 in the pathogenesis of oral submucous fibrosis","authors":"Liujun Zeng , Hui Peng , Leiye Sun , Huiqiao Yu , Yingfang Wu","doi":"10.1016/j.job.2024.100588","DOIUrl":"10.1016/j.job.2024.100588","url":null,"abstract":"<div><h3>Objectives</h3><div>Oral submucous fibrosis (OSF) is a chronic, progressive, and potentially malignant disease of the oral cavity. A previous study by our team found that the aberrant expression of tRNA-derived small RNA (tsRNA) was involved in the development of OSF, with tiRNA-Val-CAC-002 showing the most significant difference. This study aimed to investigate the effect of tiRNA-Val-CAC-002 on fibroblast activation and its underlying mechanisms, elucidate the pathogenesis of OSF, and explore new effective targets for OSF prevention and treatment.</div></div><div><h3>Methods</h3><div>RT-PCR was used to detect tiRNA-Val-CAC-002 expression in OSF and arecoline-treated fibroblasts. Western blotting, MDC staining, and transmission electron microscopy validated the effects of arecoline and 002 on fibroblast autophagy. Western blotting was used to explore the signaling pathways related to tiRNA-Val-CAC-002 in OSF.</div></div><div><h3>Results</h3><div>Arecoline promotes fibroblast (FB) activation by upregulating tiRNA-Val-CAC-002. Arecoline stimulation and tiRNA-Val-CAC-002 overexpression activated fibroblasts by promoting autophagy. tiRNA-Val-CAC-002 regulates PI3K/AKT by mediating ITGB3 expression.</div></div><div><h3>Conclusions</h3><div>Arecoline upregulates tiRNA-Val-CAC-002 expression in fibroblasts. Moreover, tiRNA-Val-CAC-002 may activate the autophagy of fibroblasts in OSF by ITGB3/PI3K/AKT pathway regulation, promoting the expression of collagen fibers.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 1","pages":"Article 100588"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142689161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Apoptotic cell death during regressive changes in salivary glands: A morphological perspective 唾液腺退行性变化过程中的细胞凋亡：形态学视角。

IF 2.6

Journal of Oral Biosciences Pub Date : 2025-03-01 DOI: 10.1016/j.job.2024.100585

Shigeru Takahashi , Akihiro Nezu , Akihiko Tanimura , Chikage Tamura , Kenji Imamachi , Tadasu Sato

{"title":"Apoptotic cell death during regressive changes in salivary glands: A morphological perspective","authors":"Shigeru Takahashi , Akihiro Nezu , Akihiko Tanimura , Chikage Tamura , Kenji Imamachi , Tadasu Sato","doi":"10.1016/j.job.2024.100585","DOIUrl":"10.1016/j.job.2024.100585","url":null,"abstract":"<div><h3>Background</h3><div>Apoptosis was initially identified through transmission electron microscopy. Subsequent advances in morphological techniques for apoptosis detection have revealed its involvement in multiple pathological conditions in various tissues. This review summarizes previous experimental studies on apoptotic cell death during regressive changes in the salivary glands, with a focus on morphological observations.</div></div><div><h3>Highlight</h3><div>Obstructive sialadenitis is histologically characterized by acinar cell loss and increased number of duct cells. Although acinar cells were previously believed to dedifferentiate into duct cells, there is evidence that they are eliminated by apoptosis. Animals fed a soft diet exhibited parotid gland atrophy, in which acinar cells decreased in size and disappeared because of apoptosis. Age-related changes in the salivary glands involved a reduced number of acinar cell through apoptosis. Additionally, apoptotic acinar cell death occurs in other pathological conditions, including the regression of hypertrophic and irradiated salivary glands.</div></div><div><h3>Conclusion</h3><div>Apoptosis often eliminates acinar cells during atrophic alterations in the salivary glands. Unlike necrosis, apoptosis is an active form of cell death, thereby helping prevent the complete destruction of the salivary glands. However, the contribution of apoptosis to regressive changes in the salivary glands remains unclear and warrants further investigation.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 1","pages":"Article 100585"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142569536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced osteogenic differentiation of human periodontal ligament cells by mature osteoclasts 成熟破骨细胞促进人牙周韧带细胞成骨分化

IF 2.6

Journal of Oral Biosciences Pub Date : 2025-02-22 DOI: 10.1016/j.job.2025.100632

Sumit Suamphan , Anupong Makeudom , Suttichai Krisanaprakornkit , Pimphorn Meekhantong , Ekapong Dechtham , Chidchanok Leethanakul

{"title":"Enhanced osteogenic differentiation of human periodontal ligament cells by mature osteoclasts","authors":"Sumit Suamphan , Anupong Makeudom , Suttichai Krisanaprakornkit , Pimphorn Meekhantong , Ekapong Dechtham , Chidchanok Leethanakul","doi":"10.1016/j.job.2025.100632","DOIUrl":"10.1016/j.job.2025.100632","url":null,"abstract":"<div><h3>Objective</h3><div>Several <em>in vitro</em> studies have shown that reverse signaling from osteoclasts regulates osteoblast differentiation and mineralization. However, none of these studies have reported the effects of this signaling pathway on periodontal ligament (PDL) cells. Therefore, in this study, we aimed to investigate the interaction between receptor activators of nuclear factor kappa B (RANK) released from mature human osteoclasts and the membranous RANK ligand (RANKL) in human PDL cells.</div></div><div><h3>Methods</h3><div>Multinucleated mature human osteoclasts were differentiated from peripheral blood mononuclear cells upon incubation with recombinant macrophage colony-stimulating factor and RANKL. Mature osteoclasts and human PDL cells were characterized. A mature osteoclast-conditioned medium (OC-CM) was used to induce osteogenic differentiation of PDL cells. Mechanistic analysis of RANK-RANKL reverse signaling were conducted to determine the regulation of osteogenic induction using conditioned medium from mature osteoclasts treated with GW4869 (GW–OC–CM) or PDL cells pretreated with recombinant human osteoprotegerin (OPG).</div></div><div><h3>Results</h3><div>OC-CM significantly upregulated the mRNA expression of osteogenic genes and enhanced the osteogenic differentiation and biomineralization of PDL cells (<em>p</em> < 0.05). GW–OC–CM significantly reduced the expression of osteogenic genes, osteogenic differentiation, and biomineralization in PDL cells (<em>p</em> < 0.05). Similarly, the pretreatment of PDL cells with OPG before OC-CM treatment significantly reduced the osteogenic induction of PDL cells (<em>p</em> < 0.05).</div></div><div><h3>Conclusion</h3><div>Mature osteoclasts can induce osteogenesis in human PDL cells via RANK-RANKL reverse signaling.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 2","pages":"Article 100632"},"PeriodicalIF":2.6,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143478645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TMPRSS2 expression in oral mucosal cells induced by transfected double-stranded RNA and IL-1β 转染双链RNA和IL-1β诱导口腔黏膜细胞TMPRSS2表达。

IF 2.6

Journal of Oral Biosciences Pub Date : 2025-02-16 DOI: 10.1016/j.job.2025.100619

Misaki Akagi , Kouji Ohta , Miyuki Sakuma , Takako Naruse , Yoko Ishida , Chieko Niwata , Nao Yamakado , Takayuki Nakagawa , Shigehiro Ono , Hiromi Nishi , Hideo Shigeishi , Tomonao Aikawa

{"title":"TMPRSS2 expression in oral mucosal cells induced by transfected double-stranded RNA and IL-1β","authors":"Misaki Akagi , Kouji Ohta , Miyuki Sakuma , Takako Naruse , Yoko Ishida , Chieko Niwata , Nao Yamakado , Takayuki Nakagawa , Shigehiro Ono , Hiromi Nishi , Hideo Shigeishi , Tomonao Aikawa","doi":"10.1016/j.job.2025.100619","DOIUrl":"10.1016/j.job.2025.100619","url":null,"abstract":"<div><h3>Objectives</h3><div>Transmembrane serine protease 2 (TMPRSS2) plays a key role in the entry of viruses such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and influenza A into host cells, and its elevated expression is a risk factor for the spread of viral infection. However, TMPRSS2 expression and the factors related to its induction in oral keratinocytes and fibroblasts remain largely unknown. Here, we examined TMPRSS2 expression and factors related to its induction in oral mucosal cells.</div></div><div><h3>Methods</h3><div>TMPRSS2 expression was examined in oral keratinocytes (RT7) and fibroblasts (GT1). Subsequently, TMPRSS2 induction in was analyzed in both cell types following transfection of nucleic acid and inflammatory cytokines, such as interleukin (IL)-1β. Finally, the effects of IL-1β on STAT1 activation related to double-stranded RNA (dsRNA)-induced TMPRSS2 expression were examined.</div></div><div><h3>Results</h3><div>RT7 and GT1 cells exhibited constitutive TMPRSS2 mRNA and protein expression. Transfection with Poly(I:C) (as a dsRNA) and poly (dA:dT) (as a double-stranded DNA [dsDNA]) increased TMPRSS2 expression. TMPRSS2 expression was also increased by IL-1β, but not IFN-γ or TNF-α, while the combination of IL-1β and transfected Poly(I:C) caused a dramatic increase in TMPRSS2 expression as compared to each alone in both cell types. IL-1β also enhanced transfected Poly(I:C)-activated STAT1 related to TMPRSS2 expression.</div></div><div><h3>Conclusions</h3><div>TMPRSS2-expressing oral keratinocytes and fibroblasts are targets of SARS-CoV-2 and influenza A virus. TMPRSS2 expression, in cooperation with IL-1β, plays an important role in promoting infection during virus invasion in oral mucosal cells.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 2","pages":"Article 100619"},"PeriodicalIF":2.6,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143450596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oral biosciences: The annual review 2024 口腔生物科学：年度回顾2024。

IF 2.6

Journal of Oral Biosciences Pub Date : 2025-02-15 DOI: 10.1016/j.job.2025.100631

Hayato Ohshima , Kentaro Ono , Kenji Mishima

{"title":"Oral biosciences: The annual review 2024","authors":"Hayato Ohshima , Kentaro Ono , Kenji Mishima","doi":"10.1016/j.job.2025.100631","DOIUrl":"10.1016/j.job.2025.100631","url":null,"abstract":"<div><h3>Background</h3><div>The <em>Journal of Oral Biosciences</em> is committed to advancing and disseminating fundamental knowledge across all areas of oral biosciences. This editorial review features review articles covering diverse topics, including the “mandible,” “tooth remineralization,” “dental pulpitis,” “dental implants,” “mesenchymal stem cells,” “microbiota,” “facial pain,” “stomatitis,” “odontogenic tumors,” “oral submucous fibrosis,” “insights on orofacial pain,” “tissue engineering,” “melatonin,” and “regenerative medicine.”</div></div><div><h3>Highlight</h3><div>This editorial review focuses on forensic anthropology, calcium sucrose phosphate, pulp biomarkers, zirconia, mesenchymal stem cells, microflora, stomatitis, ameloblastoma, areca nut, orofacial pain, collagen, melatonin, and tooth regeneration.</div></div><div><h3>Conclusion</h3><div>The review articles featured in the <em>Journal of Oral Biosciences</em> have significantly contributed to expanding readers’ knowledge across various domains of oral biosciences. The current editorial review discusses the key findings and significance of these review articles.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 1","pages":"Article 100631"},"PeriodicalIF":2.6,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143442410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MDP/NOD2 enhances RANKL-induced osteoclast differentiation of RAW264.7 cells MDP/NOD2增强rankl诱导的RAW264.7细胞破骨细胞分化。

IF 2.6

Journal of Oral Biosciences Pub Date : 2025-02-14 DOI: 10.1016/j.job.2025.100630

Wakana Sugimoto , Hiroshi Inoue , Nagako Sougawa , Seiji Goda , Aki Nishiura

{"title":"MDP/NOD2 enhances RANKL-induced osteoclast differentiation of RAW264.7 cells","authors":"Wakana Sugimoto , Hiroshi Inoue , Nagako Sougawa , Seiji Goda , Aki Nishiura","doi":"10.1016/j.job.2025.100630","DOIUrl":"10.1016/j.job.2025.100630","url":null,"abstract":"<div><h3>Objective</h3><div>Receptor activator of nuclear factor-κB ligand (RANKL) is intimately involved in regulating bone remodeling during osteoclast differentiation and promotion of osteoclast function. Upon binding to its receptor, RANK, RANKL activates various signaling cascades that induce osteoclast differentiation of osteoclast precursor cells into osteoclasts. In the innate immune system, host pattern recognition receptors, such as Toll-like receptors and nucleotide-binding oligomerization domain-like receptors (NLRs), detect pathogen-associated molecular patterns and elicit an immune response. The NLR, nucleotide-binding oligomerization domain 2 (NOD2), is known to bind muramyl dipeptide (MDP) and regulate inflammatory responses via nuclear factor-κB (NF-κB). The objective of this study was to investigate the effect of MDP on RANKL stimulation of osteoclast differentiation to elucidate the mechanism of bone resorption in a bacterial infection-induced inflammation model.</div></div><div><h3>Methods</h3><div>The extent of osteoclast formation in MDP-stimulated RAW 264.7 cells was assessed using a tartrate-resistant acid phosphatase activity assay. The protein levels of intracellular signaling molecules were assessed by western blotting.</div></div><div><h3>Results</h3><div>In RAW 264.7 cells, MDP stimulation did not affect the expression of RANK. MDP enhanced the expression of osteoclast-specific proteins, such as nuclear factor of activated T cells 1 (NFATc1) and cathepsin K, which are osteoclast differentiation markers, in RANKL-stimulated RAW 267.4 cells. Furthermore, JSH23, an NF-κB inhibitor, suppressed the expression of NFATc1 after co-stimulation with MDP and RANKL.</div></div><div><h3>Conclusion</h3><div>MDP promoted osteoclast differentiation in RAW 267.4 cells by upregulating the activators, NF-κB and NFATc1, which are important for osteoclast differentiation, through enhancement of the RANKL signaling pathway.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 1","pages":"Article 100630"},"PeriodicalIF":2.6,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143434151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The genetic basis of micro-structural fragility in murine dentin: Insights from type 2 diabetes mellitus 鼠牙本质微观结构易碎性的遗传基础：来自2型糖尿病的见解

IF 2.6

Journal of Oral Biosciences Pub Date : 2025-02-10 DOI: 10.1016/j.job.2025.100629

Hideaki Inagawa , Chie Watanabe , Jun Zhou , Yasutaka Sugamori , Noriyuki Wakabayashi , Kazuhiro Aoki , Yo Shibata

{"title":"The genetic basis of micro-structural fragility in murine dentin: Insights from type 2 diabetes mellitus","authors":"Hideaki Inagawa , Chie Watanabe , Jun Zhou , Yasutaka Sugamori , Noriyuki Wakabayashi , Kazuhiro Aoki , Yo Shibata","doi":"10.1016/j.job.2025.100629","DOIUrl":"10.1016/j.job.2025.100629","url":null,"abstract":"<div><h3>Objectives</h3><div>Diabetes mellitus (DM) is a health issue affecting millions of people worldwide. Prolonged hyperglycemia increases the risk of pathological fractures; however, verifying this risk through bone analysis is challenging because of the heterogeneity of bone.</div></div><div><h3>Methods</h3><div>The systemic effects of type 2 DM (T2DM) on calcified tissues were investigated by examining dentin in mice, focusing on the underlying cellular and molecular mechanisms. Mouse incisor dentin was selected because of its continuous growth, similar to the annual rings of wood, offering a unique opportunity to study the time-dependent deterioration of calcified tissue affected by T2DM. RNA sequencing of pulp-derived cells was used to identify transcriptomic alterations in a db/db mouse model (BKS.cg-Lepr[db]/Lepr[<em>db</em>]Jc). Structural and mechanical changes in dentin were evaluated using Raman spectroscopy and nanoindentation.</div></div><div><h3>Results</h3><div>There was an increase in dentin volume in diabetic mice, accompanied by a deterioration in mechanical properties, particularly in primary dentin. This mechanical deterioration is likely to be associated with an inflammation-driven formation of abnormal dentin matrix caused by long-term hyperglycemia. No significant differences were observed in cross-linked collagen structures or advanced glycation end products.</div></div><div><h3>Conclusions</h3><div>The findings demonstrated that gene expression in T2DM affects dentin and bone, contributing to micro-structural fragility through protein production. The incisor model used in this study proved to be a versatile tool for assessing other diseases that affect the integrity of calcified tissues over time.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 1","pages":"Article 100629"},"PeriodicalIF":2.6,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143402029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dietary texture-driven masticatory activity and its impact on stress tolerance 饮食纹理驱动的咀嚼活动及其对应激耐受性的影响。

IF 2.6

Journal of Oral Biosciences Pub Date : 2025-02-07 DOI: 10.1016/j.job.2025.100628

Mie Kamate , Hitoshi Teranishi , Ryohei Umeda , Kenshiro Shikano , Shiho Kitaoka , Toshikatsu Hanada , Takatoshi Hikida , Kenji Kawano , Reiko Hanada

{"title":"Dietary texture-driven masticatory activity and its impact on stress tolerance","authors":"Mie Kamate , Hitoshi Teranishi , Ryohei Umeda , Kenshiro Shikano , Shiho Kitaoka , Toshikatsu Hanada , Takatoshi Hikida , Kenji Kawano , Reiko Hanada","doi":"10.1016/j.job.2025.100628","DOIUrl":"10.1016/j.job.2025.100628","url":null,"abstract":"<div><h3>Objectives</h3><div>Although previous studies suggest that dietary texture-driven masticatory activity is correlated with stress tolerance, the underlying mechanisms, including neurotransmitter dynamics, remain unclear. This study investigated the effects of dietary texture-driven masticatory activity on stress tolerance in mice.</div></div><div><h3>Methods</h3><div>Behavioral responses to stress were assessed using the repeated social defeat stress (R-SDS) and social interaction test (SIT) model. Neurotransmitter levels in stress-related brain regions were analyzed in mice fed a solid diet (promoting masticatory activity) or a powdered diet (decreasing masticatory activity).</div></div><div><h3>Results</h3><div>Mice fed the powdered diet exhibited reduced stress tolerance compared with those fed the solid diet. Following the R-SDS, the powdered diet group displayed elevated gamma-aminobutyric acid (GABA) and norepinephrine levels in the prefrontal cortex. Before stress treatment, glutamic acid levels increased and those of choline decreased in the amygdala, whereas dopamine levels decreased in the powdered diet group after the R-SDS. In the locus coeruleus, mice on the powdered diet showed decreased glutamic acid and adenosine levels, alongside increased GABA levels. Serotonin levels decreased in the powdered diet group after the R-SDS, with no changes observed after the SIT. In the ventral hippocampus, GABA levels increased in the powdered diet group but decreased after the SIT.</div></div><div><h3>Conclusions</h3><div>This study demonstrates a correlation between masticatory activity and stress tolerance, evidenced by both behavioral and neurotransmitter changes. These findings suggest that reduced masticatory activity due to dietary texture contributes to decreased stress resilience.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 1","pages":"Article 100628"},"PeriodicalIF":2.6,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143383549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0