Enhanced osteogenic differentiation of human periodontal ligament cells by mature osteoclasts

IF 2.6 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences Pub Date : 2025-02-22 DOI:10.1016/j.job.2025.100632

Sumit Suamphan , Anupong Makeudom , Suttichai Krisanaprakornkit , Pimphorn Meekhantong , Ekapong Dechtham , Chidchanok Leethanakul

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引用次数: 0

Abstract

Objective

Several in vitro studies have shown that reverse signaling from osteoclasts regulates osteoblast differentiation and mineralization. However, none of these studies have reported the effects of this signaling pathway on periodontal ligament (PDL) cells. Therefore, in this study, we aimed to investigate the interaction between receptor activators of nuclear factor kappa B (RANK) released from mature human osteoclasts and the membranous RANK ligand (RANKL) in human PDL cells.

Methods

Multinucleated mature human osteoclasts were differentiated from peripheral blood mononuclear cells upon incubation with recombinant macrophage colony-stimulating factor and RANKL. Mature osteoclasts and human PDL cells were characterized. A mature osteoclast-conditioned medium (OC-CM) was used to induce osteogenic differentiation of PDL cells. Mechanistic analysis of RANK-RANKL reverse signaling were conducted to determine the regulation of osteogenic induction using conditioned medium from mature osteoclasts treated with GW4869 (GW–OC–CM) or PDL cells pretreated with recombinant human osteoprotegerin (OPG).

Results

OC-CM significantly upregulated the mRNA expression of osteogenic genes and enhanced the osteogenic differentiation and biomineralization of PDL cells (p < 0.05). GW–OC–CM significantly reduced the expression of osteogenic genes, osteogenic differentiation, and biomineralization in PDL cells (p < 0.05). Similarly, the pretreatment of PDL cells with OPG before OC-CM treatment significantly reduced the osteogenic induction of PDL cells (p < 0.05).

Conclusion

Mature osteoclasts can induce osteogenesis in human PDL cells via RANK-RANKL reverse signaling.

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成熟破骨细胞促进人牙周韧带细胞成骨分化

目的一些体外研究表明，破骨细胞的反向信号调节成骨细胞的分化和矿化。然而，这些研究都没有报道这种信号通路对牙周韧带（PDL）细胞的影响。因此，在本研究中，我们旨在研究成熟的人破骨细胞释放的核因子κ B受体激活剂（RANK）与人PDL细胞中膜性RANK配体（RANKL）的相互作用。方法用重组巨噬细胞集落刺激因子和RANKL培养人外周血单核细胞，分化成成熟的多核破骨细胞。成熟破骨细胞和人PDL细胞进行了表征。采用成熟破骨细胞条件培养基（OC-CM）诱导PDL细胞成骨分化。通过对RANK-RANKL反向信号传导的机制分析，确定GW4869 （GW-OC-CM）处理成熟破骨细胞或重组人骨保护素（OPG）预处理的PDL细胞在条件培养基中对成骨诱导的调节作用。结果soc - cm可显著上调成骨基因mRNA表达，促进PDL细胞成骨分化和生物矿化(p <；0.05)。GW-OC-CM可显著降低PDL细胞成骨基因的表达、成骨分化和生物矿化(p <；0.05)。同样，在OC-CM治疗前用OPG预处理PDL细胞可显著降低PDL细胞的成骨诱导作用(p <；0.05)。结论成熟破骨细胞可通过RANK-RANKL反向信号通路诱导人PDL细胞成骨。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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