Turkish Journal of Obstetrics and Gynecology最新文献

{"title":"The retrospective data analysis of NLRP7 and KHDC3L mutations in Turkish patients with recurrent hydatidiform mole.","authors":"Leyla Özer, Süleyman Aktuna, Evrim Ünsal","doi":"10.4274/tjod.galenos.2025.40456","DOIUrl":"10.4274/tjod.galenos.2025.40456","url":null,"abstract":"<p><strong>Objective: </strong>Recurrent hydatidiform mole (RHM) is a rare disorder which is characterized by the presence of at least two molar pregnancies. The mutations in the <i>NLRP7</i> and <i>KHDC3L</i> genes are responsible for the majority of recurrent molar pregnancies. This study aimed to demonstrate the diversity and frequency of <i>NLRP7</i> and <i>KHDC3L</i> gene mutations in our Turkish cohort with recurrent molar pregnancies, and to establish genotype-phenotype correlation.</p><p><strong>Materials and methods: </strong>It was aimed to represent the detected <i>NLRP7</i> and <i>KHDC3L</i> gene variants and reproductive history of 32 recurrent mole hydatidiform patients. We analysed the retrospective clinical and sequence data of 32 patients, who were referred to the laboratory for <i>NLRP7</i> and <i>KHDC3L</i> sequencing.</p><p><strong>Results: </strong>Among the detected 32 patients with recurrent molar pregnancy, 18 of 32 patients had no mutation in these two genes; we found 7 cases of homozygous <i>NLRP7</i> variant, 1 case of heterozygous <i>NLRP7</i>7 variant, 3 cases of homozygous <i>KHDC3L</i> gene variant, and 1 case of heterozygous <i>KHDC3L</i> gene variant. Among the detected <i>NLRP7</i> variants, 3 of 11 variants were classified as pathogenic, 7 of 11 variants were classified as likely pathogenic, and 1 of 11 variants was classified as variant of unknown significance (VUS). Among the detected <i>KHDC3L</i> variants, 1 of 4 was classified as pathogenic, 2 of 4 were classified as likely pathogenic, and 1 of 4 was classified as VUS. Seven unpublished <i>NLRP7</i> gene variants and two unpublished <i>KHDC3L</i> gene variants were first reported in this study.</p><p><strong>Conclusion: </strong>Here we report new RHM patients with <i>NLRP7</i> and <i>KHDC3L</i> mutations. The current study highlights the importance of defining new cases and novel mutations in the pathogenesis and clinical management of RHM. Understanding genotype-phenotype correlations in RHM patients will also contribute to the selection of treatment methods and patient management.</p>","PeriodicalId":45340,"journal":{"name":"Turkish Journal of Obstetrics and Gynecology","volume":"22 3","pages":"230-236"},"PeriodicalIF":1.3,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12411986/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145001652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prediction of oocyte maturity before denudation: Is the assessment of COC morphology a reliable option?","authors":"Nuri Peker, Özge Karaosmanoğlu, Ömür Albayrak, Burak Elmas, İlke Özer Aslan, Ayşen Yücetürk, Burcu Aksu Türan, Bülent Tıraş","doi":"10.4274/tjod.galenos.2025.96155","DOIUrl":"10.4274/tjod.galenos.2025.96155","url":null,"abstract":"<p><strong>Objective: </strong>We aimed to demonstrate the predictive value of morphological assessment of cumulus-oocyte complexes (COCs) prior to denudation in distinguishing mature and immature oocytes.</p><p><strong>Materials and methods: </strong>The study consisted of two stages. Five embriologists were enrolled to the first stage of the study and they divided COCs into two groups according to the morphologic features of the COS's: COCs with mature oocytes and COCs with immature oocytes. The process was overseen by one embryologist. Two hours later, COCs were denuded, and the maturity of oocytes was evaluated by another embryologist. The results were recorded. The first stage was terminated when each embryologist had evaluated a minimum of 100 COCs. In the second stage, three embryologists applied the procedure continuously for one more month. At the end of the study, the effects of continuous assessment on the prediction success were evaluated.</p><p><strong>Results: </strong>Eighty patients were enrolled in the study, and a total of 1039 COCs were examined. In the first stage of the study, 69% of immature and 80% of mature oocytes were identified correctly by the embryologists. There was no significant difference among the embryologists in terms of success rates. In the second stage of the study, the success rates of immature oocyte prediction increased for all three embryologists. However, a statistically significant increase was observed for only one embryologist (p<0.05). However, the prediction rates of mature oocytes were comparable with the results of the first stage of the study. There was no significant relationship between the number of COCs and the prediction value.</p><p><strong>Conclusion: </strong>Morphological assessment of COCs before denudation does not provide accurate results in identifying mature and immature oocytes.</p>","PeriodicalId":45340,"journal":{"name":"Turkish Journal of Obstetrics and Gynecology","volume":" ","pages":"194-198"},"PeriodicalIF":1.3,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12411985/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144973473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effect of N-acetylcysteine in doxorubicin-induced primary ovarian failure in female rats.","authors":"İlke Özer Aslan, Mehmet Öz, Hüseyin Erdal, İhsan Karaboğa, Mehmet Doğan","doi":"10.4274/tjod.galenos.2025.71654","DOIUrl":"10.4274/tjod.galenos.2025.71654","url":null,"abstract":"<p><strong>Objective: </strong>N-acetylcysteine (NAC), an aminothiol compound, eliminates free radicals and enhances glutathione (GSH) synthesis, thereby strengthening intracellular antioxidant defenses. Although its protective effects against ovarian injury have been reported, its efficacy in doxorubicin (DOX)-induced ovarian failure has not been demonstrated. This study aimed to investigate whether NAC exerts a protective role against DOX-induced ovarian toxicity in female rats.</p><p><strong>Materials and methods: </strong>Twenty-one adult female rats were randomly assigned to three groups: Control, DOX (10 mg/kg, i.p., single dose), and DOX+NAC (150 mg/kg, i.p., for 5 days; DOX administered on day 3, one hour after NAC). Serum and tissue oxidative stress parameters, histopathological changes, proliferating cell nuclear antigen (PCNA) immunoreactivity, and TUNEL assay were evaluated.</p><p><strong>Results: </strong>DOX significantly reduced serum anti-Müllerian hormone (AMH) (6.75 → 5.31 ng/mL; p<0.001) and GSH (422.64 → 280.98 mg/L; p<0.001), while increasing tumor necrosis factor alpha (TNF-α) (175.87 → 260.77 ng/L; p<0.001) and total oxidant status (TOS) (7.18 → 11.84 U/mL; p=0.002). NAC treatment reversed these alterations, namely: AMH (6.51 ng/mL; p=0.004), GSH (363.86 mg/L; p=0.018), TNF-α (184.55 ng/L; p<0.001), TOS (7.88 U/mL; p=0.003). In ovarian tissue, DOX reduced GSH (123.63 → 80.64 mg/L; p=0.001) and total antioxidant status (14.88 → 10.57 U/mL; p<0.001), while elevating TOS (7.14 → 12.64 U/mL; p<0.001) and caspase-3 (2.06 → 3.14 ng/mL; p<0.001). NAC significantly improved all these parameters (p≤0.005). Histologically, DOX caused edema, hemorrhage, infiltration, and a reduction in the percentage of healthy follicles, whereas NAC markedly ameliorated these alterations. Furthermore, NAC enhanced PCNA expression and reduced TUNEL-positive granulosa cells, supporting its anti-apoptotic effect.</p><p><strong>Conclusion: </strong>NAC preserved ovarian reserve and follicular integrity by suppressing oxidative stress, inflammation, and apoptosis induced by DOX. These findings highlight NAC as a promising protective agent against chemotherapy-induced ovarian toxicity.</p>","PeriodicalId":45340,"journal":{"name":"Turkish Journal of Obstetrics and Gynecology","volume":" ","pages":"266-274"},"PeriodicalIF":1.3,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12411988/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144973514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Routine antenatal toxoplasmosis screening, is it necessary?","authors":"Gonca Türker Ergün, Dilek Şahin","doi":"10.4274/tjod.galenos.2025.15088","DOIUrl":"10.4274/tjod.galenos.2025.15088","url":null,"abstract":"<p><strong>Objective: </strong>Toxoplasmosis is an intracellular parasite and one of the most common congenital infections. Currently, there is no clear consensus on routine screening for toxoplasma infection during pregnancy. This study aimed to discuss the results of antenatal toxoplasma screening in a tertiary center.</p><p><strong>Materials and methods: </strong>A retrospective study including the data of the antenatal toxoplasmosis screening test results over four years. Toxoplasma immunoglobulin M (IgM), toxoplasma immunoglobulin G (IgG), anti-IgG avidity test results, amniocentesis, and toxoplasma polymerase chain reaction (PCR) were obtained from the hospital records. Patients with missing toxoplasma IgM, IgG, anti-IgG avidity, test results were excluded from the study. In addition, the fetal outcomes and follow-up information for the newborns of pregnant women who gave birth in our hospital were recorded.</p><p><strong>Results: </strong>During the study period, a total of 49,292 toxoplasma IgM tests were examined. Fifty pregnant women whose toxoplasma IgM was positive with a low-anti-toxoplasma IgG avidity index were enrolled in the study group. Forty percent of the pregnant women are expected to have amniocentesis. There was only one termination of pregnancy with specific ultrasonographic findings. Toxoplasma PCR was found to be negative in the other pregnant women. Of the pregnant women who were followed up, 23 gave birth in our hospital and the Sabin Feldman test was positive in 65.2 percent (15/23) of the newborns.</p><p><strong>Conclusion: </strong>Antenatal toxoplasmosis screening should be preserved for pregnant women with fetal ultrasonographic findings which may be related to toxoplasmosis. Further studies are needed.</p>","PeriodicalId":45340,"journal":{"name":"Turkish Journal of Obstetrics and Gynecology","volume":"22 3","pages":"216-219"},"PeriodicalIF":1.3,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12411984/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145001664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential biomarkers for predicting the efficacy of a pembrolizumab-containing regimen in advanced cervical cancer: A real-world analysis.","authors":"Shintaro Yanazume, Ikumi Kitazono, Shinichi Togami, Akihide Tanimoto, Hiroaki Kobayashi","doi":"10.4274/tjod.galenos.2025.93607","DOIUrl":"10.4274/tjod.galenos.2025.93607","url":null,"abstract":"<p><strong>Objective: </strong>Prognostic biomarkers in patients with advanced cervical cancer treated with immune checkpoint inhibitors remain unclear. An evaluation of combined positive score (CPS) and tumor proportion score (TPS), and a comparison of their usefulness with inflammatory biomarkers in real-world data could be informative.</p><p><strong>Materials and methods: </strong>We analyzed 28 patients who were treated with the KEYNOTE-826 regimen between November 2022 and June 2024. The complete cohort (group 1), patients with no prior chemotherapy (group 2), and treatment-naïve (group 3) were evaluated as follows: 1) CPS, TPS, neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), and hemoglobin, albumin, lymphocyte, and platelets (HALP score) in peripheral blood samples were obtained prior to initial treatment and KEYNOTE-826 regimen, and receiver operating curve analysis was used to compare them. The optimal cut-off values that showed the highest level of discrimination for progression-free survival were identified.</p><p><strong>Results: </strong>The areas under the curve (AUC) for progression-free survival in group 2 were measured for CPS, TPS, NLR, PLR, and HALP scores before the KEYNOTE-826 regimen. The AUC values for these scores were 0.644, 0.662, 0.852, 0.667, and 0.700, respectively. The lower NLR (≤5.52) group had a significantly longer median survival than the higher NLR (>5.52) group (p˂0.001), with median survivals of 14.0 vs. 7.6 months, respectively. In group 3, CPS and TPS were highest at 0.700 for predicting progression-free survival, compared to NLR, PLR, and HALP score. CPS and TPS appear positively correlated with progression-free survival.</p><p><strong>Conclusion: </strong>CPS and TPS showed a modest correlation with progression-free survival and NLR prior to immunotherapy demonstrated the best treatment efficacy for advanced cervical cancer.</p>","PeriodicalId":45340,"journal":{"name":"Turkish Journal of Obstetrics and Gynecology","volume":"22 3","pages":"246-256"},"PeriodicalIF":1.3,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12411987/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145001625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The efficacy of single-incision laparoscopic surgery versus conventional laparoscopic surgery in the surgical management of ectopic pregnancy: A systematic review and meta-analysis.","authors":"Greg J Marchand, Daniela Herrera González, Brooke Hamilton, Mckenna Robinson, Emily Kline, Sarah Mera, Nidhi Pulicherla, Greenley Jephson, Ali Azadi","doi":"10.4274/tjod.galenos.2025.02761","DOIUrl":"10.4274/tjod.galenos.2025.02761","url":null,"abstract":"<p><p>To compare the efficacy and safety of single-incision laparoscopic surgery (SILS) versus conventional laparoscopic surgery (CLS) for the surgical management of ectopic pregnancy through a systematic review and meta-analysis. We searched Medline, PubMed, Scopus, Web of Science, ClinicalTrials.gov, and Cochrane databases from inception to May, 2023 for studies comparing SILS with CLS in ectopic pregnancy treatment. Included studies were controlled and observational, excluding single-arm studies, meta-analyses, and reviews. Quality was assessed using ROBINS-I for observational studies and the Cochrane tool for randomized trials. Data were analyzed with OpenMetaAnalyst and Review Manager 5.4.1, using odds ratios for dichotomous outcomes and mean differences (MD) for continuous outcomes. Twelve studies involving 880 women (372 SILS, 508 CLS) were included. SILS showed significantly less blood loss (MD=-51.01 mL, p=0.004), shorter postoperative hospital stay (MD=-0.24 days, p=0.003), and faster return of bowel function (MD=-1.03 hours, p<0.01), compared to CLS. No significant differences were found in total operative time, hemoglobin change, blood transfusion requirements, or number of patients needing transfusions. Patient satisfaction data were limited but suggested better cosmetic outcomes with SILS. SILS is a feasible and effective alternative to CLS for ectopic pregnancy, offering reduced blood loss, shorter hospital stays, and quicker bowel function recovery. These benefits, alongside potential cosmetic advantages, make SILS a promising option, particularly for young women. Further research is needed to confirm long-term outcomes and optimize patient selection.</p>","PeriodicalId":45340,"journal":{"name":"Turkish Journal of Obstetrics and Gynecology","volume":" ","pages":"275-286"},"PeriodicalIF":1.3,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12411975/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144973511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of pelvic floor muscle training in managing urinary incontinence in pregnant women with and without gestational diabetes mellitus.","authors":"Parisa Ghadiri Harati, Seyed Majid Hosseini, Atiyeh Javaheri, Farideh Dehghan Manshadi, Alireza Akbarzadeh Baghban","doi":"10.4274/tjod.galenos.2025.22470","DOIUrl":"10.4274/tjod.galenos.2025.22470","url":null,"abstract":"<p><strong>Objective: </strong>Urinary incontinence (UI) is a common issue during pregnancy. Pelvic floor muscle training (PFMT) may offer an effective solution for managing this condition. This study aimed to evaluate the effect of PFMT on reducing UI symptoms in pregnant women.</p><p><strong>Materials and methods: </strong>This study was conducted on 40 UI pregnant women with gestational diabetes mellitus (GDM) and 40 UI pregnant women without GDM. The participants in the experimental group were treated for 10 weeks in the third trimester, whereas the control groups received an educational pamphlet. Quality of life and UI severity were assessed using questionnaires, and pelvic floor muscle performance was measured through ultrasound-based bladder base displacement. Assessments were performed before treatment, after 10 weeks, and 2 weeks postpartum.</p><p><strong>Results: </strong>In the non-diabetic group, significant reductions in UI symptoms were observed at the end of the third trimester and 2 weeks postpartum [adjusted difference -7.56, 95% confidence interval (CI) -10.62 to -4.49, p<0.001]. However, in the diabetic group, a reduction was noted, but it was not statistically significant. Additionally, the intervention positively impacted quality of life in the non-diabetic group (adjusted difference 30.8, 95% CI 17.6 to 44.1, p<0.001) but not in the diabetic group. Notably, no significant improvement in pelvic floor muscle performance was observed in either group.</p><p><strong>Conclusion: </strong>This study suggests that PFMT can be more effective than routine pamphlets in reducing UI symptoms and improving the quality of life in pregnant women, both with and without GDM. Further research is needed to explore effects on pelvic floor muscle performance.</p>","PeriodicalId":45340,"journal":{"name":"Turkish Journal of Obstetrics and Gynecology","volume":"22 3","pages":"257-265"},"PeriodicalIF":1.3,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12411983/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145001658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Composition of the vaginal microbiota in relation to cervical intraepithelial lesions.","authors":"Ethem Serdar Yalvaç, Çağlayan Ateş, Mustafa Bakırcı, Emine Yeşilyurt Şölen, Fulya Kayıkçıoğlu, Sevgi Koç, Ayşe Erbay, Demet Aydoğan Kırmızı, Taylan Onat","doi":"10.4274/tjod.galenos.2025.09803","DOIUrl":"10.4274/tjod.galenos.2025.09803","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate the relationship between cervical intraepithelial neoplasia associated with human papillomavirus (HPV) infection and the vaginal microbiome.</p><p><strong>Materials and methods: </strong>In this study, the vaginal microbiota profile was compared among three groups of women: those with HPV infection and no cervical intraepithelial neoplasia (NILM, n=35), those with low-grade squamous intraepithelial lesions (LSIL, n=28), and those with high-grade squamous intraepithelial lesions (HSIL, n=24). Vaginal bacterial diversity was analyzed by deep sequencing of the V3-4 region of the barcoded <i>16S rRNA</i> gene using the Illumina MiSeq platform, considering alpha diversity, beta diversity, and taxon classifications. Statistical significance was set at p<0.05.</p><p><strong>Results: </strong>In the analyses performed using Chao1, Inverse Simpson, Shannon, and Observed indices, statistically significant differences were found among the groups in terms of all indices (p<0.05). Among groups, beta diversity did not show any notable differences. According to the \"Linear Discriminant Analysis Effect Size\" analysis, the taxa enriched in the HSIL group were Roseburia inulinivorans (p=0.0308), <i>Micromonosporaceae</i> family (p=0.0331), and <i>Pirellula</i> genus and species, (Planctomycetes), (p=0.0165); the taxa enriched in the LSIL group were <i>Actinobaculum</i> genus and species (p=0.0183). <i>Lactobacillus helveticus</i> and <i>Faecalibacterium prausnitzii</i> were more abundant in the NILM group, while <i>Prevotella copri, Akkermansia muciniphila</i>, and <i>Fusobacterium species</i> were more abundant in the LSIL and HSIL groups.</p><p><strong>Conclusion: </strong>Our findings indicate that variations in the severity of cervical lesions are associated with notable alterations in vaginal microbiota composition. Further research is required to conclude which contribute to the formation of the cervical lesion and which are a consequence, among those that cause changes in the vaginal microbiota, of the lesion.</p>","PeriodicalId":45340,"journal":{"name":"Turkish Journal of Obstetrics and Gynecology","volume":" ","pages":"220-229"},"PeriodicalIF":1.3,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12411972/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144817831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of conventional karyotype analysis and CMA results with ultrasound findings in pregnancies with normal QF-PCR results.","authors":"Zafer Bütün, Masum Kayapınar, Gökalp Şenol, Ece Akca, Ebru Erzurumluoğlu Gökalp, Sevilhan Artan","doi":"10.4274/tjod.galenos.2025.10673","DOIUrl":"10.4274/tjod.galenos.2025.10673","url":null,"abstract":"<p><strong>Objective: </strong>In cases requiring fetal diagnostic testing, conventional karyotype analysis is initially preferred. However, quantitative fluorescent-polymerase chain reaction (QF-PCR) or fluorescent in situ hybridization methods are used alongside conventional karyotype analysis to obtain rapid results. If results cannot be obtained from conventional karyotype analysis, chromosomal microarray analysis (CMA) is a reasonable option in necessary cases. In this study, we analyzed the conventional karyotype and CMA results from pregnancies reported as having normal karyotypes by QF-PCR and assessed their correlation with ultrasound imaging results.</p><p><strong>Materials and methods: </strong>Between 2020 and 2023, pregnant women with fetal structural anomalies detected by ultrasound and magnetic resonance imaging at the Eskişehir City Hospital, Clinic of Perinatology were referred to our prenatal diagnosis center. In samples obtained using appropriate diagnostic methods, QR-PCR and conventional karyotype analysis were performed initially. Pregnancies with chromosomal anomalies detected by QF-PCR were excluded from the study. For pregnancies with normal karyotypes, CMA was applied.</p><p><strong>Results: </strong>In 203 pregnancies with a normal karyotype result from QF-PCR, 202 (99.5%) were reported as normal in conventional karyotype analysis, while 1 (0.5%) case showed deletion of chromosome 7. Among the remaining pregnancies, CMA examination revealed abnormal karyotype results in 25 (12.3%) cases. A relationship was found only between ventriculomegaly detected by ultrasound and CMA results. The prevalence of ventriculomegaly was higher in those with CMA abnormalities (16%) compared to those with normal CMA (4.5%), and this difference was statistically significant (p=0.045).</p><p><strong>Conclusion: </strong>The benefit of CMA analysis in detecting chromosomal anomalies such as copy number variations, especially in cases reported as having a normal karyotype by QF-PCR and karyotype analysis, is evident. To evaluate the relationship between ultrasound anomalies and CMA results, each community should assess its own results.</p>","PeriodicalId":45340,"journal":{"name":"Turkish Journal of Obstetrics and Gynecology","volume":"22 2","pages":"106-113"},"PeriodicalIF":1.0,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12136108/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144217206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of lymph node metastasis in cervical cancer: A retrospective comparison of preoperative MRI and PET/CT with postoperative histopathology results.","authors":"Süleyman Özen, Ergul Demircivi, Abdulkadir Turgut, Muzaffer Sancı","doi":"10.4274/tjod.galenos.2025.27482","DOIUrl":"10.4274/tjod.galenos.2025.27482","url":null,"abstract":"<p><strong>Objective: </strong>The aim of this study is to assess the diagnostic performance of positron emission tomography/computed tomography (PET/CT) and magnetic resonance imaging (MRI) in detecting pelvic and paraaortic lymph node involvement in cervical cancer patients by correlating imaging results with surgical pathology findings.</p><p><strong>Materials and methods: </strong>A retrospective analysis was conducted on cervical cancer patients treated at İstanbul Medeniyet University Prof. Dr. Süleyman Yalçın City Hospital from 2016 to 2022. Patients who underwent preoperative PET/CT or MRI imaging and subsequent lymph node dissection were included. Sensitivity, specificity, positive predictive value, and negative predictive value were calculated for each imaging modality.</p><p><strong>Results: </strong>Of the 75 cases reviewed, 52 met the inclusion criteria. PET/CT had higher specificity (94.1%) than MRI (82.4%), while MRI demonstrated greater sensitivity (55.6% vs. 50%). False-negative rates were 15.3% for MRI and 17.3% for PET/CT. Receiver operating characteristic analysis indicated an area under the curve of 0.78 for PET/CT and 0.69 for MRI. No statistically significant differences in sensitivity or specificity were observed, with both modalities showing complementary strengths.</p><p><strong>Conclusion: </strong>MRI and PET/CT each contribute significantly to preoperative cervical cancer evaluation, with MRI favored for local assessment and PET/CT for nodal detection. Combining both modalities enhances diagnostic accuracy. Further prospective research is required to confirm and strengthen these results. and improve imaging strategies for clinical practice.</p>","PeriodicalId":45340,"journal":{"name":"Turkish Journal of Obstetrics and Gynecology","volume":"22 2","pages":"129-133"},"PeriodicalIF":1.0,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12136126/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144217209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}