Sumer 1最新文献

筛选
英文 中文
Synergistic Treatment of Candida albicans Infected Mice by AgNPsSynthesis Penicillium chrysogenum agnps合成青霉菌协同治疗白色念珠菌感染小鼠
Sumer 1 Pub Date : 2023-08-15 DOI: 10.21931/rb/css/2023.08.01.67
Noor Naeem Jasim, Rasha M. A. Al-humairi, T. Hashim Mohammad, Ruwaida A. Hussein
{"title":"Synergistic Treatment of Candida albicans Infected Mice by AgNPs\u0000Synthesis Penicillium chrysogenum","authors":"Noor Naeem Jasim, Rasha M. A. Al-humairi, T. Hashim Mohammad, Ruwaida A. Hussein","doi":"10.21931/rb/css/2023.08.01.67","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.01.67","url":null,"abstract":"Penicillium chrysogenum has been used for the production of metal\u0000nanoparticles. It was experimentally shown that silver nitrate ions are reduced to\u0000silver nanoparticles in biomass, and silver nanoparticles are synthesized from 1\u0000mM silver nitrate. The external morphology of ground AgNPs was established by\u0000SEM analysis and UV-visible spectrophotometry, and the size of AgNO3 particles was observed to be 23 nm, with particles being spherical by AFM. In vitro\u0000investigations, the antifungal susceptibility of Candida al was calculated, and the\u0000results showed that the inhibitory effect of AgNPs synthesized with P. chrysogenum is increased with increasing the concentration of nanoparticles, and the effective fungicidal concentration (EC50) was 1 ppm, and the minimum fungicidal\u0000concentration (MFC) was 100 ppm. Furthermore, the pathogenicity of C. albicans on kidneys in mice during infections was tested and thus indicated that the\u0000kidneys' ability was significantly enhanced when animals were treated with the\u0000combination of AgNPs and P. chrysogenum. This study's data from examination\u0000provide valuable preliminary statistics for using biosynthesized silver nanoparticles to manage various microorganisms.\u0000Keywords: Candida albicans, AgNPs Synthesis, Penicillium chrysogenum","PeriodicalId":443152,"journal":{"name":"Sumer 1","volume":"46 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120975568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Subchronic intraperitoneal toxicity of Sio2NPs on body weight andthyroid gland hormones in female Rats Sio2NPs对雌性大鼠体重和甲状腺激素的亚慢性腹腔毒性
Sumer 1 Pub Date : 2023-08-15 DOI: 10.21931/rb/css/2023.08.01.59
Riam Sabah Abbood, Noori M. Luaibi
{"title":"Subchronic intraperitoneal toxicity of Sio2NPs on body weight and\u0000thyroid gland hormones in female Rats","authors":"Riam Sabah Abbood, Noori M. Luaibi","doi":"10.21931/rb/css/2023.08.01.59","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.01.59","url":null,"abstract":"This study investigated the toxicity effect of Sio2NPs on body weight\u0000and thyroid gland in female rats; the experiment was included. The experimental\u0000animals were randomly divided into three groups, including two treatment groups\u0000( treated with a low dose (25mg/Kg ) and high dose (100mg/Kg of body weight)\u0000of sio2 NPs and one group as a control. Each group of them was divided into\u0000three subgroups according to the injection periods of exposure (10, 20, and 30)\u0000days daily, then measure the body weight and Thyroid function hormones (T3,\u0000T4, TSH), the results showed that there was a significant decrease (p<0.05) in the\u0000body weight of animals that exposed to SiO2 NPs at two doses (25 and 100\u0000mg/kg) during (10 days) while increased during (20, 30 days) but the highest value recorded in the animals that were treated for (30 days) at both doses. On the\u0000other side, the T3 findings recorded a significant decrease (p< 0.05) for the animals that exposed to SiO2 NPs at low and high doses (25 and 100 mg/kg) (during\u0000(10 days) when compared with the control group As well as, there was a significant decrease (p< 0.05) in the level of T3 for all animals that treated with these\u0000doses during (20 and 3 days) in serum of animals that were treated with both doses of SiO2 NPs when compared with the control group (p< 0.05), while T4\u0000showed s decreasing in the treated group during (10 and 20, 30 days) compared\u0000to a control group with highly significant ((p< 0.05), finally while TSH showed a\u0000highly increasing in treated group during (10 and 20, 30 days) compared to control group (p< 0.05). The study revealed that Exposure to Sio2NPs at different\u0000concentrations caused structural and functional and in the thyroid gland, characterized decrease in T3 and T4 hormone levels and an increase in the TSH hormone level and caused hypothyroidism This led to a decrease in the metabolism\u0000process and thus the body weight of animals gained a significant increase.\u0000Keywords: nanoparticles, Sio2NPs, Thyroid gland, T3, T4, TSH, Body weight,\u0000female rats.","PeriodicalId":443152,"journal":{"name":"Sumer 1","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123541474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Prevalence of parallel infection of dental caries and tonsillitis amongsome Iraqi children 一些伊拉克儿童龋齿和扁桃体炎并行感染的流行
Sumer 1 Pub Date : 2023-08-15 DOI: 10.21931/rb/css/2023.08.01.84
Dunia B. Shabeeb, Eman N. Naji
{"title":"Prevalence of parallel infection of dental caries and tonsillitis among\u0000some Iraqi children","authors":"Dunia B. Shabeeb, Eman N. Naji","doi":"10.21931/rb/css/2023.08.01.84","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.01.84","url":null,"abstract":"Aims and Objectives: This study aims to investigate the role of dental\u0000caries-causing bacteria in increasing acute or chronic tonsillitis among Iraqi children. Materials and Methods: 120 patients were part of the study and were divided into 6 groups; each group had 20 patients aged 6–12. Swab samples were obtained from dental plaque and tonsils to identify the bacterial flora. Identification\u0000methods included metabolic capability, microscopic reading, and biochemical reaction. Results: The results showed that Streptococcus pneumonia was the most\u0000common Gram-positive bacterial species isolated from three different groups and\u0000control rate (32.9 %). Also, Moraxella catarrhalis, Granulicatella elegant and\u0000Streptococcus parasanguinis appeared in both tonsillitis and dental caries. Conclusion: An interesting association between dental caries and tonsillitis in children aged 6 to 12 appeared. Most Gram-positive and Gram-negative bacterial isolates were multi-drug resistant to antimicrobial agents.\u0000Keywords: parallel infection, dental caries, tonsillitis, Iraqi children.","PeriodicalId":443152,"journal":{"name":"Sumer 1","volume":"50 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122712205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of adding different cinnamon oil concentrations on thechemical, physical, and microbial properties of local goose liver meatduring different storage periods 添加不同肉桂油浓度对不同贮藏期本地鹅肝肉化学、物理和微生物特性的影响
Sumer 1 Pub Date : 2023-08-15 DOI: 10.21931/rb/css/2023.08.01.76
Adnan Shakor Ahmed Al-Perkhdri, Sami Ibrahim Abdullah, Ahmed Sami Shaker
{"title":"Effect of adding different cinnamon oil concentrations on the\u0000chemical, physical, and microbial properties of local goose liver meat\u0000during different storage periods","authors":"Adnan Shakor Ahmed Al-Perkhdri, Sami Ibrahim Abdullah, Ahmed Sami Shaker","doi":"10.21931/rb/css/2023.08.01.76","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.01.76","url":null,"abstract":"Cinnamon oil is a plant extract used to exert antimicrobial actions\u0000against essential pathogens. 96 samples of goose liver were used and divided for\u0000four treatments (control, T1=0.025, T2=0.050, T3=0.075) for three different storage times (1, 15, 20 days). After the storage periods, finished samples were analyzed, including chemical traits (carbohydrate, protein, ash, and moisture) and\u0000physical traits (pH, water holding capacity, loss during cooking, and loss during\u0000throwing). Also, a microbial trait was measured (cold bacteria and total bacteria).\u0000Significant differences were found just in (pH, loss during throwing, cold bacteria, and total bacteria (0.000, 0.046, 0.000, and 0.000), respectively, in the three\u0000different storage periods. There were statistically significant differences between\u0000the different levels of cinnamon oil and the storage periods, which affected the\u0000microbial number. Further studies are needed to test other goose meat parts with\u0000different cinnamon levels.\u0000Keywords: Liver, meat, storage, chemical, physical, microbiological","PeriodicalId":443152,"journal":{"name":"Sumer 1","volume":"84 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126407377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Histological Detection and Anti-oxidant Effect of Bee Venom on thePancreas of Diabetic Mice 蜂毒对糖尿病小鼠胰腺的组织学检测及抗氧化作用
Sumer 1 Pub Date : 2023-08-15 DOI: 10.21931/rb/css/s2023.08.01.45
R. A. Al-Sarray, S. Al-Shaeli
{"title":"Histological Detection and Anti-oxidant Effect of Bee Venom on the\u0000Pancreas of Diabetic Mice","authors":"R. A. Al-Sarray, S. Al-Shaeli","doi":"10.21931/rb/css/s2023.08.01.45","DOIUrl":"https://doi.org/10.21931/rb/css/s2023.08.01.45","url":null,"abstract":"Bee venom BV draws attention in the medical field to manage several diseases,\u0000including type 2 diabetes mellitus (T2DM). Due to limited research on this field in\u0000Iraq, the study aimed to detect BV's histological and anti-oxidant impact in diabetic\u0000mice. Fifteen male mice were randomly assigned into three groups. The first group\u0000is control (C), the second is diabetic, and the last group is BV. At the end of 30\u0000days, the obtained serum was used to measure the level of glucose, insulin, superoxide dismutase (SOD), and malondialdehyde (MDA). Furthermore, the pancreas\u0000was histologically assessed using the Gomori stain. Alloxan showed significant\u0000elevation and reduction in the level of glucose and insulin compared to the control.\u0000These alterations were positively enhanced by BV. BV promoted significant elevation in the activity of SOD and suppressed MDA compared to diabetic mice.\u0000Histologically, diabetic mice pancreas displayed a reduction of capsular tissue, islets and beta cells vacuolization, beta cells initial degeneration, reduction in the\u0000shape and size of the islets and beta cells, and vascular hemorrhage. These changes\u0000were remarkably enhanced nearly to normal by BV. Therefore, BV could control\u0000the diabetic condition, which may be considered a potential anti-diabetic agent.\u0000Keywords: Diabetes mellitus, SOD, MDA, pancreas, mice, BV","PeriodicalId":443152,"journal":{"name":"Sumer 1","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133083355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sweet and Salt Threshold Detection among Dental Implant Patientsin Relation to Cognition Status and Salivary Acetylcholinesterase 种植牙患者糖、盐阈值检测与认知状态及唾液乙酰胆碱酯酶的关系
Sumer 1 Pub Date : 2023-08-15 DOI: 10.21931/rb/css/2023.08.01.80
Rihab Abdul Hussein Ali, Ban Sahib Diab
{"title":"Sweet and Salt Threshold Detection among Dental Implant Patients\u0000in Relation to Cognition Status and Salivary Acetylcholinesterase","authors":"Rihab Abdul Hussein Ali, Ban Sahib Diab","doi":"10.21931/rb/css/2023.08.01.80","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.01.80","url":null,"abstract":"Background: Wearing a dental implant may affect gustatory function,\u0000an essential element of health in older adults that can affect cognition status. This\u0000study aimed to assess the sweet and salt taste threshold detection among dental\u0000implant patients in relation to cognition status and salivary Acetylcholinesterase\u0000compared with partially edentulous and fully dentate patients matching in age\u0000and gender. Materials and methods: The study comprised 90 dental implant patients between the ages of 50 and 60 compared with 90 partially edentulous and\u000090 fully dentate individuals who were age and gender-matched. The participants'\u0000cognition status was tested using the Self-Administered Gero-cognitive Examination (SAGE) test, which classified them into poor and good. A two-alternative\u0000forced-choice question was used to establish taste threshold sensitivity, with concentrations presented in ascending order. One chemical was investigated for each\u0000taste quality (salt and sweet), and each component was delivered at five different\u0000concentrations. The enzyme-linked immunosorbent test (ELISA) kit measured\u0000salivary Acetylcholinesterase. Spss 22 Chicago Illionis USA was used to examine the data. Results: There was a significant association between sweet and salt\u0000taste thresholds and the patient's groups, as the higher patient occurrence concerning the lowest taste threshold was found for the dentate patients.\u0000In comparison, the higher patient occurrence for the highest taste threshold was\u0000found for the partially edentulous patients. Data analysis showed a significant association between cognitive status and sweet and salt taste threshold for the implant patient group, as the percentage of patients with the lowest taste threshold\u0000was higher for good cognitive status; the same result was found concerning partially edentulous and fully dentate patients; however, the association was not significant. The results revealed that the mean value of salivary Acetylcholinesterase\u0000(AChE) was higher among the fully dentate group, followed by implant. Then,\u0000the partially edentulous groups with statistically insignificant differences for both\u0000poor and good cognitive status groups. On the other hand, when comparing the\u0000poor and good cognitive status groups, results revealed that the mean value of\u0000salivary Acetylcholinesterase was higher among the good than the poor groups,\u0000with statistically significant differences among all sample groups. Conclusion:\u0000Within the limitation of this investigation, it was confirmed that taste threshold\u0000detection is connected with missing teeth and cognition status. The findings show\u0000a complicated interplay of several factors that affect gustatory acuity, including\u0000oral health and cognition status.\u0000Keywords: Threshold, Dental Implant Patients, Salivary Acetylcholinesterase","PeriodicalId":443152,"journal":{"name":"Sumer 1","volume":"39 3","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132090845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Study the Genetic Performance of Some Faba Bean Genotypes Under MosulCondition, Iraq. 伊拉克摩苏尔条件下几种蚕豆基因型遗传性能的研究。
Sumer 1 Pub Date : 2023-08-15 DOI: 10.21931/rb/css/s2023.08.01.23
Esraa Abd-Alhuseein Jasim, Kamal Benyamin Esho, Nagham Salah Salim
{"title":"Study the Genetic Performance of Some Faba Bean Genotypes Under Mosul\u0000Condition, Iraq.","authors":"Esraa Abd-Alhuseein Jasim, Kamal Benyamin Esho, Nagham Salah Salim","doi":"10.21931/rb/css/s2023.08.01.23","DOIUrl":"https://doi.org/10.21931/rb/css/s2023.08.01.23","url":null,"abstract":"Throughout the agricultural season fall 2020/2021, research has been carried out\u0000in the vegetable research field of the Dept. of Horticulture and Landscaping,\u0000Univ. of Mosul, to investigate the genetic performance of several Faba bean genotypes under consideration of Mosul conditions. Seeds of 7 bean cultivars were\u0000sown (S2009-175, FBSPN2, Aguadulce, ILD1814, Histal, Favada Orio, and\u0000Luzde otono). The genotypes differed considerably in all of the examined variables at a probability threshold of 5%, according to the results of an ANOVA table\u0000analysis of sources of variance. The genotype FBSPN2 has been superior in characteristics of plant height and seed yields regarding each of the experimental\u0000units. S2009-175 produced the maximum number of dry pods for each plant,\u0000Favada Orio in the trait of seed length and pod weight, and Histal produced the\u0000maximum diameter of a pod and number of seeds in each one of the pods and diameter of seed for each experimental unit. The genetic and phenotypic variance\u0000was high for the weight of the pods for each one of the plants, the weight of 100\u0000seeds, and the height of the plant, according to the results of the genetic parameter analysis, and the percentage of heritability in a broader sense had exceeded\u000060% for the traits, number of pods, and plant length. For the experimental unit,\u0000dry pod weight for each plant, dry pods per plant, seed diameter, pod length and\u0000diameter, dry seed yield, and 100-seed weight were all high, and genetic improvement for pod weight, seed weight, and weight of a 100-seeds was high as\u0000well.\u0000Keywords: Faba bean, Seed properties, Genetic parameter, Genotypes, Heritability","PeriodicalId":443152,"journal":{"name":"Sumer 1","volume":"102 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128668823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Oxytocin and Interleukin-14: New suggested indicators for prediction of type 2 diabetes-induced nephropathy 催产素和白细胞介素-14:预测2型糖尿病肾病的新指标
Sumer 1 Pub Date : 2023-08-15 DOI: 10.21931/rb/css/2023.08.01.15
Rasha Hasan Jasim, Mohammed Abbas Khulaibus
{"title":"Oxytocin and Interleukin-14: New suggested indicators for prediction of type 2 diabetes-induced nephropathy","authors":"Rasha Hasan Jasim, Mohammed Abbas Khulaibus","doi":"10.21931/rb/css/2023.08.01.15","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.01.15","url":null,"abstract":"Diabetes is one of the major risk factors for chronic renal failure (CRF). The present work involved studying the effect of lifestyle factors such as (age, gender,\u0000smoking and area of residence) which can affect chronic renal diseases. Also, the\u0000study estimated Oxytocin (Oxt) and Interleukin-14 (IL-14) using the sandwich\u0000ELISA technique in the serum of type 2 diabetic patients with CRF who presented to hemodialysis and a control group. Blood samples of (50) diagnosed type 2\u0000diabetic patients with CRF from both sexes were compared with (40) samples\u0000from healthy individuals as a control group. The results demonstrated significant\u0000elevation (p<0.05) in Oxt and IL-14 concentrations for patients with CRF compared to the control group. As for the subgroups, the statistical test showed a significant difference (p < 0.05) when comparing Oxt levels between the same sex\u0000in the two groups. While no significant difference was observed when comparing\u0000IL-14 in the same or the two groups. The parameters Oxt and IL-14 are necessary\u0000tools for predicting renal failure.\u0000Keywords: Oxytocin, Interleukin-14, Type 2 Diabetes Mellitus, Hemodialysis","PeriodicalId":443152,"journal":{"name":"Sumer 1","volume":"18 ","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114095475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Polymerization of sunflower oil with adipic acid using Catalyst atdifferent temperatures 向日葵油与己二酸在不同温度下的聚合反应
Sumer 1 Pub Date : 2023-08-15 DOI: 10.21931/rb/css/2023.08.01.91
Safa fakhri Kadouri, Ghalib Adrees Atiya Ali, Abdul Muniem Abbas Karim
{"title":"Polymerization of sunflower oil with adipic acid using Catalyst at\u0000different temperatures","authors":"Safa fakhri Kadouri, Ghalib Adrees Atiya Ali, Abdul Muniem Abbas Karim","doi":"10.21931/rb/css/2023.08.01.91","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.01.91","url":null,"abstract":"The polymers that have been prepared include the following:\u0000Poly(glyceride-ALT-adipate), which symbolizes it, has been prepared in two\u0000steps: The first step: Prepare the monomer from the reaction of glycerin with sunflower oil (glyceride reaction) in the presence of catalysts (pbO2) at a temperature of 150 and using the Electrical Stirrer for 5 hours. The second step: This step\u0000includes the polymerization process (condensation) in order to prepare (Alkyd\u0000Resin), where the monomer (Monoglyceride) is prepared by reacting with (Adipic acid) at different temperatures starting from (150 to 240 with a difference of 10\u0000℃ between one reaction and another using an electronic thermometer to control\u0000the temperature and with continuous stirring using a magnet bar and in the presence of nitrogen gas (inert atmosphere). The reaction kinetics is also calculated,\u0000which includes two processes. The first process, the kinetics of the reaction, is\u0000made by tracking the decrease in the concentration of acid value, which is made\u0000by the polymerization process.\u0000Keywords: sunflower oil, adipic acid, PbO2 Catalyst.","PeriodicalId":443152,"journal":{"name":"Sumer 1","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125286603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Biological activities of purified prolidase from pathogenic E.coli 致病性大肠杆菌纯化脯氨酸酶的生物学活性
Sumer 1 Pub Date : 2023-08-15 DOI: 10.21931/rb/css/2023.08.01.57
Seror A. Abdul Hussein, Neihaya H. Zaki, Khetam H.Rasool
{"title":"Biological activities of purified prolidase from pathogenic E.coli","authors":"Seror A. Abdul Hussein, Neihaya H. Zaki, Khetam H.Rasool","doi":"10.21931/rb/css/2023.08.01.57","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.01.57","url":null,"abstract":"Prolidase is a ubiquitous enzyme that plays a chief role in the metabolism of proline-rich proteins. The goal of this study extraction and purify\u0000prolidase from E. coli and evaluate some applications such as anti-biofilm and\u0000anticancer. Hundred stool Samples were collected from infants with breastfeeding, non-vomiting, and non-diarrhea to isolate E.coli bacteria. A 16S rRNA gene\u0000(585 bp) was found in all isolates of E. coli via PCR identification. Depending on\u0000the qualitative method on prolidase agar, only 40 (80%) isolates could produce\u0000prolidase from 50 isolates that were considered non-pathogens, then only 32 isolates revealed different levels in prolidase production with specific activity equal\u0000to (2.1U/mg) of E.coli.\u0000MS12. Sucrose, casein, and 40Co were the chosen isolate's best conditions for\u0000producing prolidase. Cold acetone precipitation and dialysis were used to extract\u0000the enzyme, and DEAE-cellulose and the Sephadex G-150 column were used in\u0000purification with specific activity (2 U/ml) and (6.6 U/mg) protein. Prolidase\u0000showed the highest effect on biofilm at 500 μg/ml concentration against P. aeruginosa, then E. coli, 65% and 60.3% respectively. Brain Tumor Cell Line (A127),\u0000Colorectal Adenocarcinoma cells (CaCo-2), and Normal embryonic liver cell line\u0000(WRL-68) were used to test the prolidase effect on these cell lines. An assay of\u0000MTT was used to detect the inhibiter concentration (IC50) values and cytotoxic\u0000effect of purified prolidase.\u0000Keywords: E.coli, Prolidase, purification, antibiofilm, anticancer activity","PeriodicalId":443152,"journal":{"name":"Sumer 1","volume":"143 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133697990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信