Galen Medical Journal最新文献

{"title":"Puncture site bleeding complications of peripheral nerve block in patients taking antithrombotic and anticoagulant drugs: An umbrella review","authors":"Ahmad Rastgarian, Khatereh Dehghani, Shahram Shafa, Mohammad Sadegh Sanie Jahromi, Mansour Deylami, Soha Azizi, Mohammad Zarenezhad, Kaveh Hedayati Emami, Vahid Rahmanian, Tayyebeh Zarei, Navid Kalani","doi":"10.31661/gmj.v12i.2958","DOIUrl":"https://doi.org/10.31661/gmj.v12i.2958","url":null,"abstract":"background: While there are multiple guidelines for the management of bleeding complications and hematoma if being treated with antithrombotic and anticoagulant drugs, these risks are not yet stratified for procedures with regional anesthesia. Materials and Methods: This study was an umbrella review of systematic studies and meta-analysis based on PRISMA guidelines in databases of Scopus, PubMed, Medline, Cochrane Library, and Web of Science databases. Due to heterogeneity in evaluated outcomes and methods of studies, only the qualitative evidence synthesis was performed. AMSTAR checklist was used to assess the risk of bias in included systematic reviews. Results: After an extensive search of relevant studies, 971 primary cases were identified. Following a thorough screening process, 5 systematic reviews were selected. The evidence suggests that head and neck punctures generally do not result in bleeding complications, except for rare cases of hematoma associated with Infraclavicular brachial plexus block. A deep cervical plexus block is not recommended. Interscalene blocks have varying findings, with some studies reporting hematoma incidence and spinal injury, while others consider them low risk. Supraclavicular brachial plexus block might be associated with hemothorax and infraclavicular blocks are not favored by reviews. Axillary brachial plexus blocks have a minor incidence of hematoma. Abdomen blocks, TAP blocks, ilioinguinal blocks, and rectus sheath blocks carry a higher risk of hematoma. Pectoral nerve (PECS) blocks have a relatively high risk, while paravertebral and intercostal blocks are considered high risk, but further research is needed regarding paravertebral blocks. Conclusion: The available evidence from systematic reviews and studies suggests varying levels of risk for different blocks and procedures that should be considered before decision-making.","PeriodicalId":44017,"journal":{"name":"Galen Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136070209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Histopathological Findings in Appendectomy Specimens in an Iranian Population","authors":"Mahmoud Agholi, Farideh Esfandiari, Hamid Reza Heidarian, Fatemeh Khajeh, Zahra Sharafi, Ehsan Masoudi, Mohammad Rayani","doi":"10.31661/gmj.v12i.2482","DOIUrl":"https://doi.org/10.31661/gmj.v12i.2482","url":null,"abstract":"Background: Appendicitis is one of the most common causes of acute abdominal surgeries. The importance of parasitic etiologies in the pathogenesis of appendicitis is not well known in appendectomy specimens on a large scale in southwestern Iran. The current study aimed to retrospectively assess the demographic data and histopathological records of appendicitis in a 28-year period in Fars province, southwestern Iran. Materials and Methods: Histopathological records of 13,013 patients who had undergone surgeries for appendicitis at Dr. Ali Shariati Hospital, affiliated with the Fasa University of Medical Sciences from December 1993 to January 2021 were reviewed and data concerning the patients' demographic data and histopathological records were retrieved from each record. More than 6800 archived microscopic glass slides were also reviewed. Results: From a total of 13,013 histopathological records of surgical excisions of appendicitis that were reviewed over a 28-year period, 8,189 (62.9%) were male and 4,842 (37.1%) were female. Patients' age ranged from 2 to 98 years, with a mean age of 24.68±19.87 years. The most common inflammatory changes were 5,687 (43.7%), 1,228 (9.4%), 670 (5.1%), 522 (4%), and 363 (2.8%) cases of acute appendicitis, suppurative appendicitis, early acute appendicitis, gangrenous appendicitis, and perforated appendicitis respectively. Microscopically, no viral inclusions, fungal elements, and histopathologic findings of bacterial causes were found. Parasitic infections such as helminthiasis were detected in 74 (0.6%) cases aged from 6 to 63. Enterobiasis (Syn. oxyuriasis, pinworm infection) accounted for 73 (98.6%) of the 74 helminthiases, while ascariasis accounted for 1 (1.4%). Out of 74 cases, 29 (39.2%) showed evidence of appendicitis. Conclusion: The results suggest that although parasitic agents are minor causes of appendicitis, these agents should be kept in mind during differential diagnosis. However, whether every parasitic infection leads to appendicitis is controversial.","PeriodicalId":44017,"journal":{"name":"Galen Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135459173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acute or Short-term Effects of Whey Protein Alone or Along with Carbohydrate on Inflammation: A Systematic Review of Clinical Trials:.","authors":"Ali Akbari, Mahsa Moazen, Siavash Babajafari, Seyedeh Maryam Abdollahzadeh, Maryam Ranjbar Zahedani, Najmeh Sasani, Asma Kazemi","doi":"10.31661/gmj.v12i.2441","DOIUrl":"https://doi.org/10.31661/gmj.v12i.2441","url":null,"abstract":"BACKGROUND\u0000Excessive inflammatory response is associated with several diseases. Recently, there has been an increasing trend for investigation of the acute or short-term effects of whey protein alone or in combination with carbohydrates on inflammatory status, especially in athletes. This systematic review aimed to clarify these effects.\u0000\u0000\u0000MATERIALS AND METHODS\u0000PubMed, Scopus, and Web of Science databases were searched from January 1990 to September 2021, without language restriction. Adult studies examining the effects of whey protein alone or together with carbohydrates on interleukin-6, tumor necrosis factor-α, and C-reactive protein levels with a maximum duration of 15 days and with at least one comparison group were included. The quality of studies was analyzed using the Cochrane risk of bias tool.\u0000\u0000\u0000RESULTS\u0000Twenty-five studies met the inclusion criteria. Significant reductions in inflammatory markers was observed in seven out of 25 studies (28%). However, one out of 25 studies (4%) reported a significant increase in inflammatory status. Among those studies comparing the effects of whey protein alone with non-protein or protein-containing groups, 18.18% (two out of 11) and 10% (one out of ten) of the trials revealed a significant decrease in the markers, respectively. Moreover, of those studies comparing whey protein plus carbohydrate with non-protein or protein-containing groups, 33.33% (two out of six) and 40% (two out of five) of them showed a significant reduction in the inflammatory response, respectively. The quality of the majority of studies (84%) was poor.\u0000\u0000\u0000CONCLUSION\u0000It seems that whey protein alone or the combination of it with carbohydrates may not affect the inflammatory markers in the short run (PROSPERO registration number: CRD42021273915).[GMJ.2023;12:e2441].","PeriodicalId":44017,"journal":{"name":"Galen Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2023-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10506851/pdf/GMJ-12-e2441.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41143155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Use of Artificial Intelligence in the Management of Neurodegenerative Disorders; Focus on Alzheimer's Disease","authors":"Khazar Ghasempour Dabbaghi, Zahra Khosravirad, Sheida Jamalnia, Rahil GhorbaniNia, Fatemeh Mahmoudikohani, Habib Zakeri, Solmaz Khastehband","doi":"10.31661/gmj.v12i.3061","DOIUrl":"https://doi.org/10.31661/gmj.v12i.3061","url":null,"abstract":"Recent advances in artificial intelligence (AI) have shown great promise in the diagnosis, prediction, treatment plans, and monitoring the progression of neurodegenerative disorders. AI algorithms can analyze large amounts of data from various sources, including medical images, quantifiable proteins in urine, blood, and cerebrospinal fluid (CSF), genetic information, clinical records, Electroencephalography (EEG) signals, driving behaviors, etc. Alzheimer's disease as one of the most common neurodegenerative disorders. This study specifically explores the possible application of AI in the diagnosis, prediction, monitoring of disease progression, classifying, finding new biomarkers and drugs, and personalizing treatment plans of AD.","PeriodicalId":44017,"journal":{"name":"Galen Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134948035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effectiveness of Solution-Focused Brief Counseling on Marital Intimacy in Mothers of Children with Down Syndrome: A Randomized Clinical Trial","authors":"Mahshid Bokaie, Naeimeh Mirshafieian, Mir Saeid Jafari","doi":"10.31661/gmj.v12i.2747","DOIUrl":"https://doi.org/10.31661/gmj.v12i.2747","url":null,"abstract":"Background: Parents of children with intelligence and motor problems, including Down syndrome have to spend more time babysitting resulting in less intimacy with their mates. Solution-focused brief therapy is one of the treatments presented in the field of marital intimacy. This study aimed to investigate the effectiveness of solution-focused counseling on marital intimacy in mothers of children with Down syndrome. Materials and Methods: In this randomized clinical trial study, 72 couples were selected among members of the Asemannili Society (Isfahan-Iran) from 19/01/2021 to 20/04/2021. The control group received an educational pamphlet for four sessions (without homework) every other week while the intervention group attended eight 90-minute online counseling programs once a week. Bagarozi Marital Intimacy Questionnaire was completed at baseline, after intervention (8th week), and follow-up period (12th week) by the women and their spouses. Results: The mean scores of marital intimacy between the two groups at baseline (online: 313.23 ± 70.86, pamphlet: 315.92 ± 41.45) compared to the 12th week (online: 370.13 ± 44.63, pamphlet: 332.42 ± 30.39) were significantly different. The analysis of the variance test with repeated observations showed that the effect of group, and time on the total score of marital intimacy and its other dimensions, were significant (P<0.05) for women.Conclusion: Both online and pamphlet counseling can improve marital intimacy in mothers of children with Down syndrome, but online counseling appears to be more effective. Thus, this method is recommended for improving the marital intimacy of these women.","PeriodicalId":44017,"journal":{"name":"Galen Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134968376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Regulation of Pyroptosis and Ferroptosis by MicroRNAs in Cardiovascular Diseases","authors":"Akram Shariati, Venous Shahabi Raberi, Mehdi Masumi, Ali Tarbiat, Elham Rastgoo, Reza Faramarzzadeh","doi":"10.31661/gmj.v12i.2933","DOIUrl":"https://doi.org/10.31661/gmj.v12i.2933","url":null,"abstract":"Cardiovascular diseases (CVDs) are considered the most prevalent noncommunicable disease and the leading cause of death worldwide. A plethora of evidence has revealed that microRNAs (miRNAs) could control the inhibition or progression of CVDs by regulating pivotal cell processes ranging from metabolism and homeostasis to programmed cell death (PCD). Pyroptosis and ferroptosis are two major types of nonapoptotic PCDs involved in the pathogenesis of heart failure. However, no study has discussed the crosstalk between miRNAs and these two types of PCDs in the CVDs. The current review demonstrated that different types of miRNAs can regulate both ferroptosis and pyroptosis and thereby affect CVDs progression and inhibition. Altogether, the discussed content encourages further studies to confirm that mentioned pathways are suitable to be considered as novel therapeutic approaches against CVDs.","PeriodicalId":44017,"journal":{"name":"Galen Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136239817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Regulation of Pyroptosis and Ferroptosis by MicroRNAs in Cardiovascular Diseases.","authors":"Akram Shariati, Venus Shahabi Raberi, Mehdi Masumi, Ali Tarbiat, Elham Rastgoo, Reza Faramarz Zadeh","doi":"10.31661/gmj.v12i0.2933","DOIUrl":"10.31661/gmj.v12i0.2933","url":null,"abstract":"<p><p>Cardiovascular diseases (CVDs) are considered the most prevalent noncommunicable disease and the leading cause of death worldwide. A plethora of evidence has revealed that microRNAs (miRNAs) could control the inhibition or progression of CVDs by regulating pivotal cell processes ranging from metabolism and homeostasis to programmed cell death (PCD). Pyroptosis and ferroptosis are two major types of nonapoptotic PCDs involved in the pathogenesis of heart failure. However, no study has discussed the crosstalk between miRNAs and these two types of PCDs in the CVDs. The current review demonstrated that different types of miRNAs can regulate both ferroptosis and pyroptosis and thereby affect CVDs progression and inhibition. Altogether, the discussed content encourages further studies to confirm that mentioned pathways are suitable to be considered as novel therapeutic approaches against CVDs.</p>","PeriodicalId":44017,"journal":{"name":"Galen Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11227648/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141555658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Apoptosis-related Genes and Hormone Secretion Profiles Using Three Dimensional Culture System of Human Testicular Organoids.","authors":"Aghbibi Nikmahzar, Farnaz Khadivi, Morteza Koruji, Mehrdad Jahanshahi, Masoomeh Dehghan Tarazjani, Maryam Shabani, Yasaman Abbasi, Mehdi Abbasi","doi":"10.31661/gmj.v12i0.2805","DOIUrl":"10.31661/gmj.v12i0.2805","url":null,"abstract":"<p><strong>Background: </strong>In reproductive biology, testicular organoids can be used to treat infertility and to study testicular development and spermatogonial stem cells (SSCs) differentiation. Generating organoid from primary cells is challenging. In this study, testicular organoids were created using human primary testicular cells and evaluated the apoptotic gene expression and hormone secretion profiles of the organoids.</p><p><strong>Materials and methods: </strong>Primary human testicular cells were isolated using 2-step enzymatic digestion from three brain-dead donors. Immunocytochemistry and flow cytometry analyses were performed to confirm human SSCs. Isolated cells were cultured in three experimental groups: control group (2 dimensional (2D)), group 1 (organoid culture after 2D culture), and group 2 (organoid culture immediately after enzymatic digestion). Testicular organoids were cultured in DMEM/F-12 media supplemented with follicle-stimulating hormone (FSH) and fetal bovine serum (FBS) for four weeks. After 24 hours and four weeks of culture, reverse transcription quantitative real-time PCR (RT-qPCR) was used to investigate the relative expression of apoptotic genes (caspase 3, 9, Bax, and Bcl-2). At 24 hours, two weeks, and four weeks after culture, enzyme-linked immunoassay (ELISA) was used to determine the testosterone and inhibin B concentrations. Light microscopy and toluidine blue staining were also used for morphological analysis.</p><p><strong>Results: </strong>RT-qPCR results revealed that pro-apoptotic (caspase 3, 9, Bax) gene expression levels were highest in group 2 after 24 h and four weeks of culture. In contrast, the expression level of Bcl-2 (anti-apoptotic) was lower in group 2 compared to other groups. The hormone secretion levels decreased in a time-dependent manner during the cultivation. According to morphological evaluations, testicular organoids are compact, spherical structures with two to three elongated cells organized along their border.</p><p><strong>Conclusion: </strong>Our findings revealed that the testicular organoid culture system maintained hormonal secretory abilities, demonstrating the function of Sertoli and Leydig cells in the absence of testis-specific environments.</p>","PeriodicalId":44017,"journal":{"name":"Galen Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11108674/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141077134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Apoptosis-Related Genes and Hormone Secretion Profiles Using Three Dimensional Culture System of Human Testicular Organoids","authors":"A. Nikmahzar, F. Khadivi, M. Koruji, M. Jahanshahi, Masoomeh Dehghan Tarazjani, Maryam Shabani, Yasaman Abbasi, M. Abbasi","doi":"10.31661/gmj.v12i.2805","DOIUrl":"https://doi.org/10.31661/gmj.v12i.2805","url":null,"abstract":"Background: In reproductive biology, testicular organoids can be used to treat infertility and to study testicular development and spermatogonial stem cells (SSCs) differentiation. Generating organoid from primary cells is challenging. In this study, testicular organoids were created using human primary testicular cells and evaluated the apoptotic gene expression and hormone secretion profiles of the organoids. Materials and Methods: Primary human testicular cells were isolated using 2-step enzymatic digestion from three brain-dead donors. Immunocytochemistry and flow cytometry analyses were performed to confirm human SSCs. Isolated cells were cultured in three experimental groups: control group (2 dimensional (2D)), group 1 (organoid culture after 2D culture), and group 2 (organoid culture immediately after enzymatic digestion). Testicular organoids were cultured in DMEM/F-12 media supplemented with follicle-stimulating hormone (FSH) and fetal bovine serum (FBS) for four weeks. After 24 hours and four weeks of culture, reverse transcription quantitative real-time PCR (RT-qPCR) was used to investigate the relative expression of apoptotic genes (caspase 3, 9, Bax, and Bcl-2). At 24 hours, two weeks, and four weeks after culture, enzyme-linked immunoassay (ELISA) was used to determine the testosterone and inhibin B concentrations. Light microscopy and toluidine blue staining were also used for morphological analysis. Results: RT-qPCR results revealed that pro-apoptotic (caspase 3, 9, Bax) gene expression levels were highest in group 2 after 24 h and four weeks of culture. In contrast, the expression level of Bcl-2 (anti-apoptotic) was lower in group 2 compared to other groups. The hormone secretion levels decreased in a time-dependent manner during the cultivation. According to morphological evaluations, testicular organoids are compact, spherical structures with two to three elongated cells organized along their border. Conclusion: Our findings revealed that the testicular organoid culture system maintained hormonal secretory abilities, demonstrating the function of Sertoli and Leydig cells in the absence of testis-specific environments.","PeriodicalId":44017,"journal":{"name":"Galen Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2023-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83299057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Persian Medicine Network (PMN) Establishment in the Universal Scientific Education and Research Network (USERN)","authors":"Babak Daneshfard, Ebrahim Khadem","doi":"10.31661/gmj.v12i.3066","DOIUrl":"https://doi.org/10.31661/gmj.v12i.3066","url":null,"abstract":"-","PeriodicalId":44017,"journal":{"name":"Galen Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135619273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}