{"title":"Biodegradation of Sodium Dodecyl Sulphate (SDS) by Serratia marcescens strain DRY6","authors":"A. Othman, M. Yusof, M. Shukor","doi":"10.54987/bstr.v7i2.486","DOIUrl":"https://doi.org/10.54987/bstr.v7i2.486","url":null,"abstract":"A bacterium capable of degrading sodium dodecyl sulphate (SDS) is characterized. Previously, the bacterium has been shown to have the capability to reduce molybdenum to molybdenum blue. In this report, we showed that almost complete degradation of SDS was observed in 6 to 10 days when the bacterium was grown on medium supplemented with SDS ranging from 0.5 to 1 g L-1 while higher concentrations showed partial degradation with no degradation was observed at concentrations higher than 2.5 g L-1. Other detergents were also tested including Tergitol NP9, Tergitol 15S9, Witconol 2301 (methyl oleate), sodium dodecylbenzene sulfonate (SDBS), benzethonium chloride and benzalkonium chloride. However, growth can only be seen on the anionic SDBS. We also showed that the presence of metal ions such as silver, copper, cadmium, chromium, lead and mercury inhibits the ability of the bacterium to degrade SDS by 50%. Growth on SDS could not support molybdenum-reduction in this bacterium. Growth kinetic studies showed that the growth rate could be modelled using Haldane substrate inhibition kinetics with the maximum growth rate, µmax, was 0.13 h-1, while the saturation constant or half velocity constant Ks and inhibition constant Ki, were 0.707 and 11.303 g L-1 SDS, respectively.","PeriodicalId":436607,"journal":{"name":"Bioremediation Science and Technology Research","volume":"139 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133823446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nur Syafiqah Ramli, Aa’isyah Abdul Gafar, M. Shukor
{"title":"Bacillus sp. UPM-AAG1 for The Bioremediation of Ammonia in Aquaculture Wastewater","authors":"Nur Syafiqah Ramli, Aa’isyah Abdul Gafar, M. Shukor","doi":"10.54987/bstr.v7i2.484","DOIUrl":"https://doi.org/10.54987/bstr.v7i2.484","url":null,"abstract":"The widespread activity of the aquaculture industry has led to environmental pollution resulting from the uncontrollable production of ammonia from aquaculture activities. The presence of ammonia in the environment is a major threat due to its toxicity that can bring harm to organism especially aquatic organisms. Bioremediation is an efficient technique for the remedy of ammonia pollution as it provides complete assimilation of the ammonia. In this work, a bacterial isolate identified as Bacillus strain UPM-AAG1 shows the best performance out of the four isolates screened for remediating ammonia. The characterization of Bacillus strain UPM-AAG1 was conducted to identify the optimum conditions for ammonia utilization by Bacillus strain UPM-AAG1. The optimum pH for ammonia remediation by Bacillus strain UPM-AAG1 was determined to be acidic at pH 6.0. While the optimum condition for ammonia concentration was determined to be at 10 mg/mL. For the optimum temperature for ammonia remediation by Bacillus strain UPM-AAG1 was determined to be at 30 oC.","PeriodicalId":436607,"journal":{"name":"Bioremediation Science and Technology Research","volume":"80 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115002321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mohamed Nazri, A. A. Basirun, M. Manogaran, S. Khalidi, M. K. Sabullah, M. Shukor
{"title":"Optimisation of Acetylcholinesterase Extraction from the Brain of Clarias batrachus using Response Surface Methodology","authors":"Mohamed Nazri, A. A. Basirun, M. Manogaran, S. Khalidi, M. K. Sabullah, M. Shukor","doi":"10.54987/bstr.v7i2.485","DOIUrl":"https://doi.org/10.54987/bstr.v7i2.485","url":null,"abstract":"Acetylcholinesterase is an important enzyme in biosensing works. It is utilized to detect insecticides and cheaper sources of the enzyme is constantly being sought. Optimisation of the acetylcholinesterase extraction from the brain of Clarias batrachus using Response Surface Methodology is reported for the first time in this study. AChE from the brain of this fish was extracted and partially purified using ammonium sulphate. The optimum range of ammonium sulphate cut was between 40 and 50%. RSM was conducted using Box-Behnken design and the optimisation value for the extraction was determined for each variable at the end of the study. The optimum concentrations of Triton X-100, PMSF, phosphate buffer and the pH for AChE extraction based on RSM were 0.75%, 1.00 mM, 0.075 M and 7.5, respectively.","PeriodicalId":436607,"journal":{"name":"Bioremediation Science and Technology Research","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131162794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Sha'arani, Muhammad Arif Mukhriz Ros Saidon Khudri, A. Othman, M. Halmi, N. A. Yasid, M. Shukor
{"title":"Kinetic Analysis of the Adsorption of the Brominated Flame Retardant 4-bromodiphenyl Ether onto Biochar-immobilized Sphingomonas sp.","authors":"S. Sha'arani, Muhammad Arif Mukhriz Ros Saidon Khudri, A. Othman, M. Halmi, N. A. Yasid, M. Shukor","doi":"10.54987/bstr.v7i1.457","DOIUrl":"https://doi.org/10.54987/bstr.v7i1.457","url":null,"abstract":"4-bromophenyl phenyl ether or 4-bromodiphenyl ether (4-BDE) is a less brominated PBDE, which are brominated flame retardants (BFRs), that has come into importance when it was detected as the main pollutant in Sungai Buah, a tributary to the Sungai Semenyih that have recently caused the pollution of drinking waters in the Semenyih Water Treatment Plant, Putrajaya Selangor that led to water shortage for many days to affecting many areas. 4-BDE is also a recalcitrant priority pollutant where it is barely degraded at all. Many of the PBDE can cause developmental neurotoxicity. The absorption kinetics data of biosorption isotherm on the biosorption of 4-BDE onto Biochar-immobilized Sphingomonas sp. were analyzed using three models—pseudo-1st, pseudo-2nd and Elovich, and fitted using non-linear regression. The Elovich model was the poorest in fitting the curve based on visual observation followed by the pseudo-1st order. Statistical analysis based on root-mean-square error (RMSE), adjusted coefficient of determination (adjR2), bias factor (BF), accuracy factor (AF), corrected AICc (Akaike Information Criterion), Bayesian Information Criterion (BIC) and Hannan–Quinn information criterion (HQC) that showed that the pseudo-1ST order model is the best model. Kinetic analysis using the pseudo-1st order model at 400 mg/L 4-BDE gave a value of equilibrium sorption capacity qe of 31.89 mg g-1 (95% confidence interval from 30.37 to 33.42) and a value of the pseudo-1st-order rate constant, k1 of 0.22 (95% confidence interval from 0.019 to 0.025). Further analysis is needed to provide proof for the chemisorption mechanism usually tied to this kinetic.","PeriodicalId":436607,"journal":{"name":"Bioremediation Science and Technology Research","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114078607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Modelling the Effect of Heavy Metal on the Growth Rate of an SDS-degrading Pseudomonas sp. strain DRY15 from Antarctic soil","authors":"M. Manogaran, A. Othman, M. Shukor, M. Halmi","doi":"10.54987/bstr.v7i1.463","DOIUrl":"https://doi.org/10.54987/bstr.v7i1.463","url":null,"abstract":"The SDS-degrading bacterium Pseudomonas sp. strain DRY15 was strongly inhibited by heavy metals especially mercury. Growth of the SDS-degrading bacterium at various concentrations of mercury shows a sigmoidal pattern with lag periods ranging from 7 to 10 h. As the concentration of mercury was increased, the overall growth was inhibited with 1.0 g/L causing an almost cessation of bacterial growth. The modified Gompertz model was utilized to obtain growth rates at different concentrations of mercury. The growth rates obtained from the modified Gompertz model was then modelled according to the modified Han-Levenspiel, Wang, Liu, modified Andrews, the Amor and Shukor models. Out of the five models, only the Shukor, Wang, modified Han-Levenspiel and the Liu models were able to fit the curve, whilst the modified Andrews and Amor models were unable to fit the curves. The best model was Shukor based on the lowest values for RMSE and AICc, highest adjusted correlation coefficient (adR2) and values of AF and BF closest to unity. The parameters obtained from the Shukor model, which are Sm, ïmax and n which represent critical heavy metal ion concentration (mg/L), maximum growth rate (h-1) and empirical constant values were 6.0 (95%, confidence interval from 5.87 to 6.14), 0.09 (95%, confidence interval of 0.086 to 0.096) and 4.2 (95%, confidence interval from 3.1 to 5.2), respectively.","PeriodicalId":436607,"journal":{"name":"Bioremediation Science and Technology Research","volume":"16 9","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114118158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. A. Gafasa, S. Ibrahim, A. Babandi, N. Abdullahi, D. Shehu, M. Ya’u, K. Babagana, J. Mashi, H. Yakasai
{"title":"Characterizing the Molybdenum-reducing Properties of Pseudomonas sp. locally isolated from Agricultural soil in Kano Metropolis Nigeria","authors":"M. A. Gafasa, S. Ibrahim, A. Babandi, N. Abdullahi, D. Shehu, M. Ya’u, K. Babagana, J. Mashi, H. Yakasai","doi":"10.54987/bstr.v7i1.462","DOIUrl":"https://doi.org/10.54987/bstr.v7i1.462","url":null,"abstract":"Pollution of the environment by heavy metals and other toxic xenobiotics has increasingly become global public health concern. Bacterial reduction of molybdenum to insoluble molybdenum blue (Mo-blue) forms the basis for its bioremediation. A bacterium with the ability to reduce toxic soluble molybdenum has been isolated from Agricultural soil and identified as Pseudomonas sp. based on the 16S rRNA partial sequencing and phylogenetic analysis. Spectroscopic analysis reveals that the bacterium reduced sodium molybdate to Mo-blue optimally at pH between 6.5 and 7.0, temperatures between 35 °C and 40 °C. Glucose was the best electron donor source supporting molybdate reduction, followed by sucrose, fructose, glycerol and starch in descending order. Other requirements include a phosphate concentration of 3.5 mM and a molybdate concentration of between 40 and 60 mM. The absorption spectrum of the Mo-blue produced was similar to the previously isolated Mo-reducing bacteria and closely resembles a reduced phosphomolybdate.","PeriodicalId":436607,"journal":{"name":"Bioremediation Science and Technology Research","volume":"46 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116203399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hartinie Marbawi, Muhammad Arif Mukhriz Ros Saidon Khudri, A. Othman, M. Halmi, J. Gansau, N. A. Yasid
{"title":"Kinetic Analysis of the Adsorption of Glyphosate onto Palm Oil Fronds Activated Carbon","authors":"Hartinie Marbawi, Muhammad Arif Mukhriz Ros Saidon Khudri, A. Othman, M. Halmi, J. Gansau, N. A. Yasid","doi":"10.54987/bstr.v7i1.461","DOIUrl":"https://doi.org/10.54987/bstr.v7i1.461","url":null,"abstract":"Existing techniques for the treatment of pollutants include membrane separation, ion exchange, precipitation, transformation and biosorption. Of all of this technology, biosorption has several positive aspects which include low operating expenses, very efficient detoxification of toxicants at low concentrations, low amount of disposal materials and does not need nutrient requirements as in bacterial-based remediation, the latter of which is limited by the presence of heavy metals and other toxicants. The biosorption of glyphosate on palm oil fronds activated carbon can be an efficient and low-cost tool for remediation of glyphosate. The absorption kinetics data of biosorption isotherm on the biosorption of glyphosate on palm oil fronds activated carbon were analyzed using three models—pseudo-1st, pseudo-2nd and Elovich, and fitted using non-linear regression. The Elovich model was the poorest in fitting the curve based on visual observation followed by the pseudo-1st order. Statistical analysis based on root-mean-square error (RMSE), adjusted coefficient of determination (adjR2), bias factor (BF), accuracy factor (AF), corrected AICc (Akaike Information Criterion), Bayesian Information Criterion (BIC) and Hannan–Quinn information criterion (HQC) that showed that the pseudo-second order model is the best model. Kinetic analysis using the pseudo-second order model at 250 mg/L glyphosate gave a value of equilibrium sorption capacity qe of 94.12 mg g-1 (95% confidence interval from 89.913 to 98.332) and a value of the pseudo-second-order rate constant, k2 of 0.02 (95% confidence interval from 0.012 to 0.023). Further analysis is needed to provide proof for the chemisorption mechanism usually tied to this kinetic.","PeriodicalId":436607,"journal":{"name":"Bioremediation Science and Technology Research","volume":"66 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130562955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Mohammed, M. A. Gafasa, H. Kabara, A. Babandi, D. Shehu, M. Ya’u, S. Abubakar, K. Babagana, J. Mashi, H. Yakasai
{"title":"Soluble Molybdenum Reduction by Morganella sp. Locally-isolated from Agricultural Land in Kano","authors":"S. Mohammed, M. A. Gafasa, H. Kabara, A. Babandi, D. Shehu, M. Ya’u, S. Abubakar, K. Babagana, J. Mashi, H. Yakasai","doi":"10.54987/bstr.v7i1.456","DOIUrl":"https://doi.org/10.54987/bstr.v7i1.456","url":null,"abstract":"Intensive agriculture and industrial activities have significantly increased the global burden of pollutants; thus, bioremediation of these pollutants is intensely sought. A bacterium with potential of reducing toxic soluble molybdenum to precipitable molybdenum blue (Mo-blue) was isolated from agricultural soil in Darmanawa, Kano state. The bacterium grown on low phosphate media (LPM) reduces molybadate to Mo-blue optimally at pH between 6.0 and 7.5, temperature of 35 °C, glucose was the best electron donor source at 5 g/L and ammonium sulphate was the best nitrogen source. The optimum molybdate concentration supporting the reduction process was 40 mM at 3.5 mM phosphate. Phylogenetic analysis of 16S rRNA partial sequence identified the bacterium as Morganella sp. The ability of this isolate to reduce toxic soluble molybdenum to colloidal less toxic form is novel and makes the bacterium an important instrument for bioremediation of this pollutant.","PeriodicalId":436607,"journal":{"name":"Bioremediation Science and Technology Research","volume":"835 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117080305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mohd Syahmi Ramzi Salihan, Syahir Habib, F. Dahalan, N. Hassan, M. Syed, S. A. Gani, M. Shukor
{"title":"Allochthonous Diesel Biodegradation by Bacillus sp. MO1 Isolated from Diesel-contaminated Soil","authors":"Mohd Syahmi Ramzi Salihan, Syahir Habib, F. Dahalan, N. Hassan, M. Syed, S. A. Gani, M. Shukor","doi":"10.54987/bstr.v7i1.459","DOIUrl":"https://doi.org/10.54987/bstr.v7i1.459","url":null,"abstract":"Ten bacterial isolates were successfully isolated from pristine and contaminated areas in Malaysia. There was no lag phase observed signifying each of these isolates was able to quickly induce cellular machinery for diesel assimilation. The best isolate was tentatively identified as Bacillus sp. strain MO1 based on carbon utilisation profiles using Biolog GP plates and partial 16S rDNA matching. Strain MO1 grew optimally at 37 oC on 4% (v/v) diesel, pH 7.5 and 0.9% (w/v) KNO3. Diesel components were proven to be completely eliminated from the reduction in the hydrocarbon peaks monitored by gas chromatographic analysis after 6 days of incubation. The bacterium was found to be a better remediation agent than a commercial bioremediation product. All of these characteristics suggest that this bacterium is the suitable bacterium for bioremediation of diesel spills and pollution in the tropics.","PeriodicalId":436607,"journal":{"name":"Bioremediation Science and Technology Research","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116072319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Growth Inhibition Kinetics of a Pseudomonas Diesel-degrading Strain from Antarctica","authors":"F. Dahalan, N. Hassan","doi":"10.54987/bstr.v7i1.458","DOIUrl":"https://doi.org/10.54987/bstr.v7i1.458","url":null,"abstract":"Antarctica is one of the largest southernmost continent and most pristine wilderness areas left on earth. Over decades, human activities in this area have resulted in the accumulated pollution of hydrocarbon in the Antarctica mainly due to transportation and logistics activities. The sinking of the supply ships Nella Dan and Bahia Paraiso have resulted in diesel spillage that warrant the utilization and research on diesel-degrading microorganisms in the form of bioremediation to prepare for future disasters. A previously isolated diesel-degrading Pseudomonas sp. strain DRYJ3 has shown effectiveness as a bioremediation tool. Its growth is however strongly inhibited as the diesel concentrations was increased. In this study the inhibitory effect of diesel on the growth rate of this bacterium is modelled according to the Luong, Aiba, Haldane, Hans-Levenspiel, Yano, Teissier and Monod models. Statistical evaluations indicated that the most suitable kinetic model to fit the growth rate on diesel was Luong’s model. The Luong’s constants; maximal growth rate, half saturation constant for maximal growth, maximum substrate concentration that growth ceases, and curve parameter that defines the steepness of the growth rate decline from the maximum rate symbolized by ïmax, Ks, Sm, and n were 0.406 hr-1 (95% CI, 0.269 to 0.881), 0.194 (%v/v) (95% CI, 0.2877 to 0.390), 4.025 (%v/v) (95% CI, 3.820 to 4.229) and 0.378 (95% CI, 0.122 to 0.877) 0.099, respectively. The Luong model predicted Sm value was close to the value of which no growth was observed experimentally suggesting the appropriateness of the model in adhering to observed values.","PeriodicalId":436607,"journal":{"name":"Bioremediation Science and Technology Research","volume":"132 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132370057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}