Bioremediation Science and Technology Research最新文献

{"title":"Isothermal Modelling on the Removal of Copper Using Durvillaea antarctica","authors":"S. Sha'arani, N. A. Yasid, Muhammad Arif Mukhriz Ros Saidon Khudri, Mohd Yamin Shaari, M. Shukor, A. Othman","doi":"10.54987/bstr.v7i1.460","DOIUrl":"https://doi.org/10.54987/bstr.v7i1.460","url":null,"abstract":"Heavy metals pollution in the Antarctica is emerging as an important problem due to anthropogenic activities. Bioremediation of metal pollution especially copper in Antarctica in the future using biosorption is the most environmental-friendly method. Biosorption using Durvillaea antarctica, an alga that lives in the Antarctic region does not involve the introduction of new potentially foreign species. In this study, the isotherms of copper biosorption by the alga is modelled according to various models ranging from one to five parameters models such as Henry, Langmuir, Dubinin-Radushkevich, Freundlich, BET, Toth, Sips, Fritz-Schlunder IV, Baudu and Fritz-Schlunder V. All gave visually acceptable fitting with the exception of the Henry model. Statistical analysis based on root-mean-square error (RMSE), adjusted coefficient of determination (adjR2), bias factor (BF), accuracy factor (AF) and corrected AICc (Akaike Information Criterion) showed that the Sips model is the best model. The calculated Sips parameters kS value of 12.52 (95% confidence interval from 3.593 to 21.439), qmS value of 0.97 (95% confidence interval from 0.905 to 1.026) and nS value of 0.67 (95% confidence interval from 0.531 to 0.806).","PeriodicalId":436607,"journal":{"name":"Bioremediation Science and Technology Research","volume":"58 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127980892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mathematical Modeling of Molybdenum Blue Production from Bacillus amyloliquefaciens strain KIK-12","authors":"K. I. Karamba, H. Yakasai","doi":"10.54987/bstr.v6i2.435","DOIUrl":"https://doi.org/10.54987/bstr.v6i2.435","url":null,"abstract":"Molybdenum reduction to molybdenum blue is a detoxification process, and production of Mo-blue is growth associated. Mathematical modelling of the reduction process can reveal important parameters such as specific reduction rate, theoretical maximum reduction and whether reduction at high molybdenum concentration affected the lag period of reduction. The used of linearization method through the use of natural logarithm transformation, although popular, is inaccurate and can only give an approximate value for the sole parameter measured; the specific growth rate. In this work, a variety of models for such as logistic, Gompertz, Richards, Schnute, Baranyi-Roberts, Von Bertalanffy, Buchanan three-phase and more recently Huang were utilized to obtain values for the above parameters or constants from Bacillus amyloliquefaciens strain KIK-12. The Mo-blue production from this bacterium was sigmoidal in shape with a lag phase of about 15 hours and reaching maximum Mo-blue production at approximately 50 hours of static incubation. The resultant fitting shows visually acceptable fitting. The best performance was modified Gompertz model based on statistical tests such as root-mean-square error (RMSE), adjusted coefficient of determination (R2), bias factor (BF), accuracy factor (AF) and corrected AICc (Akaike Information Criterion). The modified Gompertz model was then utilized to model the Mo-blue production curves to obtain reduction coefficients. The parameter constants successfully developed from this work will be very useful for the development of further secondary models.","PeriodicalId":436607,"journal":{"name":"Bioremediation Science and Technology Research","volume":"69 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122753847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial Activity and Phytochemical Analysis of Cassia occidentalis Leaf Extract on Salmonella Typhimurium","authors":"A. I. Bagega, A. Usman, S. M. Dankaka, M. Amina, A. Mani","doi":"10.54987/bstr.v6i2.431","DOIUrl":"https://doi.org/10.54987/bstr.v6i2.431","url":null,"abstract":"Different concentration extracts of Cassia occidentalis (Caesalpiniaceae) leaves were screened for their antimicrobial activity against Salmonella Typhimurium. The pattern of inhibition was varied with the concentration used. Antibacterial activity of the extracts at different concentrations on the S. Typhimurium was recorded with no activity at 20 mg/mL. Nevertheless, at concentrations of 40, 60, 80 and 100 mg/mL, activity was observed. This activity was seen increased as the concentration increases. At the minimum bactericidal concentration (MBC) of 60 mg/mL, the bacteria were killed with no tidal activity at 30 mg/mL. The antibacterial activity of Cassia occidentalis leaf extract was recorded at concentrations of 40 mg/mL (12 mm), 60 mg/mL (15 mm), 80 mg/mL (16 mm) and 100 mg/mL (18 mm) with a minimum inhibitory concentration (MIC) of 30 mg/mL and MBC of 60 mg/mL. This was due to the presence of phytochemical compounds. Some of these compounds were found in a trace amount, whereas saponin was present high amount, flavonoids were present in a moderate amount, while tannins, glycoside, cardiac glycosides, steroids, saponin glycoside, anthraquinones and volatile oil were present in trace amount. However, balsam and alkaloid are not detected.","PeriodicalId":436607,"journal":{"name":"Bioremediation Science and Technology Research","volume":"104 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132784303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biosurfactant Production by Bacteria Isolated from Hydrocarbon-impacted soil","authors":"Ismail H.Y, T. Isa, Ngoshe I.Y, Gajere H.M, H. Ismail","doi":"10.54987/bstr.v6i2.430","DOIUrl":"https://doi.org/10.54987/bstr.v6i2.430","url":null,"abstract":"Advancement in biotechnology and its industrial application has led to increased demand for biological compounds of microbial origin. Such compounds are biosurfactant and their application in different sectors is increasing despite some challenges. In the present study, the ability of bacterial species growing in hydrocarbon impacted soil to produce biosurfactants was investigated with a view to isolate competent biosurfactant producers with desirable qualities for a large scale biosurfactant production. Soil samples contaminated with different kinds of hydrocarbon were collected from Mechanic workshop (MW) and Mai-kose (MK) area in Maiduguri Metropolis, Nigeria. Soil samples were cultured on nutrient agar and mineral salt agar by pour plate technique for enumeration of viable bacteria and isolation of oil utilising bacteria respectively. Samples from MW were observed to harbour fewer bacteria than MK area with 7.3 ×107 CFU/g and 4.0×108 CFU/g respectively. Fifteen bacterial species were isolated and identified to belong to the genus Pseudomonas using cultural, morphological and biochemical characteristics. The species were P. aeruginosa, P. citronellolis and P. mendocina with 66.7%, 26.6% and 6.6% rate of occurrence. A preliminary investigation for the isolates’ ability to produce biosurfactants using haemolysis and hydrocarbon degradation tests revealed that all the isolates were haemolytic on blood agar and can utilise vegetable oil at different magnitudes. Biosurfactant assay using broth culture supernatants showed that all the isolates were able to produce surfactants that collapsed oil drop within the interval of 10-20 min and Pseudomonas citronellolis WB4 was most efficient in this respect (t = 10 min). With regards to oil spreading assay, P. aeruginosa KB1 produced largest diameter (d) of 4.21 cm after 15 min compared to other species (dav. = 3.44cm average tav. = 13 min). After 24 hrs of emulsion formation, it was observed that P. aeruginosa WB5 (E24 = 70.45%) and P. citronellolis WB4 (E24 = 70.35%) have the highest emulsification indexes (range 70.45% - 43.75%). Therefore, all isolates in this study were biosurfactant producers with P. citronellolis WB4 being the most suitable candidate for large-scale biosurfactant production. Hence, further studies are recommended to harness its full potentials. ","PeriodicalId":436607,"journal":{"name":"Bioremediation Science and Technology Research","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114756930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbiological and Physicochemical Analysis of Old Sokoto Abattoir Wastewater (Sewage) Contaminated with Blacksmith Activities","authors":"Dankaka S.M, Farouq A.A, Bagega A.I, U. Abubakar","doi":"10.54987/bstr.v6i2.432","DOIUrl":"https://doi.org/10.54987/bstr.v6i2.432","url":null,"abstract":"The physicochemical and microbiological characteristics of wastewater samples from Old Sokoto Abattoir in Sokoto North Local Government Area, Sokoto state, Nigeria were studied over four weeks period. The physicochemical parameters of the wastewater samples studied namely pH, temperature, biochemical oxygen demand (BOD), dissolved oxygen (DfO), phosphate, nitrate, and chemical oxygen demand (COD) were within the standard limit for wastewater to be discharged into the environment. Heavy metals like nickel, copper, and lead were not detected (ND) except for iron with a concentration range from 0-0.64 mg/L. Wastewater samples for week 1 had a higher bacterial count of 270x105 CFU/mL than wastewater samples for the other weeks. The total viable count for all the samples exceeded the limit of 1x102 CFU/mL, which was between 4.1x105 – 27.0x105. All the wastewater samples were found to harbour coliform organisms in numbers greater than the required WHO standard (1x102 CFU/mL) for water. The MPN count of wastewater was ranged from 190-1100 MPN/100 mL. The bacterial species isolated were E. coli (33.3%), E. aerogenes (20%), S. aureus (6.66%), K. pneumoniae (6.66%), Proteus vulgaris (13.3%), Citrobater freundii (6.66%), Bacillus licheniformis (6.66%), and Bacillus spp. The fungal isolates were Mucor spp (12.5%), Aspergillus niger (37.5%), Aspergillus terreus (12.5%), Sporothrix schenkii (12.5%), Rhodotorula spp. (12.5%), and Penicillium spp. (12.5%). This study showed that the isolation and population of microorganisms from wastewater in Old Sokoto Abattoir is an indication that the wastewater was highly polluted with microbial load and can cause an environmental hazard if not treated.","PeriodicalId":436607,"journal":{"name":"Bioremediation Science and Technology Research","volume":"200 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128401641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temperature Coefficient and Q10 Value Estimation for the Growth of Molybdenum-reducing Serratia sp. strain HMY1","authors":"H. Yakasai, N. A. Yasid, M. Shukor","doi":"10.54987/bstr.v6i2.434","DOIUrl":"https://doi.org/10.54987/bstr.v6i2.434","url":null,"abstract":"A relative 10 ËšC increase in the surrounding temperature, usually results in doubling the reaction rate, with corresponding Q10 value of 2, which is true for a number of biological reactions. Molybdenum reduction to molybdenum blue by bacteria is one of the methods to combat the toxicity of soluble molybdenum to the bacteria. The Q10 values for molybdenum bioreduction, which can be determined from the Arrhenius plots has not previously been reported. A logarithmic plot of growth rate or reduction rates for Serratia sp. strain HMY1 against 1000/temperature (Kelvin) and the slope of the Arrhenius curve was used to obtain the Q10 value in this study. The Q10 value of 2.038 and a theta value of 1.08 obtained in this work, are within the normal range for many biological values.","PeriodicalId":436607,"journal":{"name":"Bioremediation Science and Technology Research","volume":"98 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121472050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and Characterization of a Heavy Metal-reducing Pseudomonas sp. strain Dr.Y Kertih with the Ability to Assimilate Phenol and Diesel","authors":"Veeraswamy Kesavan, A. Mansur, Z. Suhaili, Mohd Syahmi Ramzi Salihan, M. F. Rahman, M. Shukor","doi":"10.54987/bstr.v6i1.394","DOIUrl":"https://doi.org/10.54987/bstr.v6i1.394","url":null,"abstract":"The indiscriminate released of heavy metals and xenobiotics into soils and aquatic bodies severely alter soil organisms and the ecosystem. The isolation of xenobiotics degrading microorganisms is cost-effective and naturally pleasant approach. Lately, the toxicological effect of molybdenum to the spermatogenesis of several organisms has been record. This present study is aimed at the isolation and characterization of a bacterium capable of converting molybdenum to the colloidal molybdenum blue. Bacteria characterization was performed in a microplate format using resting cells. Thus, the reduction process can be employed as a device for molybdenum bioremediation.  The results of the study revealed an optimum reduction at pH between 6.0 and 6.3 and temperatures of between 25 and 40 oC. Similarly, it was also observed that a phosphate concentration not greater than 5.0 mM and a sodium molybdate concentration at 20 mM was required for reduction. Glucose was observed as the best carbon source to support reduction. Following the scanning of molybdenum blue, it revealed an absorption spectrum indicating the characteristics of molybdenum blue as a reduced phosphomolybdate. Molybdenum reduction is inhibited by heavy metals like silver, lead, arsenic and mercury. Furthermore, the ability of the bacterium (Pseudomonas sp. strain Dr.Y Kertih) to utilize several organic xenobiotics such as phenol, acrylamide, nicotinamide, acetamide, iodoacetamide, propionamide, acetamide, sodium dodecyl sulfate (SDS) and diesel as electron donor sources for aiding reduction or as carbon sources for growth was also examined. Finding showed that none was capable of aiding molybdenum reduction, however the bacterium was capable of growing on both diesel and phenol as carbon sources. GC analysis was used to confirmed diesel degradation.","PeriodicalId":436607,"journal":{"name":"Bioremediation Science and Technology Research","volume":"86 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132826724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mathematical Modelling of the Growth Curve of Vibrio sp. Isolate MZ Grown in Seawater Medium","authors":"Maryam Zahaba, M. Halmi, A. Othman, M. Shukor","doi":"10.54987/bstr.v6i1.397","DOIUrl":"https://doi.org/10.54987/bstr.v6i1.397","url":null,"abstract":"Pollution in the environment is deteriorating the ecology due to human activities in a large array of industrial and agricultural sectors. Bioassay of polluted waters using bioluminescent bacterium has been touted as one of the most economical, rapid and sensitive tests. The growth of the bacterium on seawater medium exhibited a typical sigmoidal profile. To extract important growth parameters useful for further modelling exercise, various primary growth models were utilized in this study such as Modified Logistic, modified Gompertz, modified Richards, modified Schnute, Baranyi-Roberts, von Bertalanffy, Huang and the Buchanan three-phase model. The best performance was Huang model with the lowest value for RMSE, AICc and the highest value for adjusted R2. The AF and BF values were also excellent for the model with their values were the closest to 1.0. The Huang parameters, which include A or Y0 (bacterial growth lower asymptote), m (maximum specific bacterial growth rate),  (lag time) and Ymax (bacterial growth upper asymptote) were 7.866 (95% confidence interval of 7.850 to 7.883), 0.329 (95% confidence interval of 0.299 to 0.359), 1.543 (95% confidence interval of 1.303 to 1.784) and 8.511 (95% confidence interval of 0.299 to 0.359).","PeriodicalId":436607,"journal":{"name":"Bioremediation Science and Technology Research","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130628508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacterial Inhibition Activity of Methanolic Extract from Salvia officinalis: Determination of the IC50 value by Nonlinear Regression","authors":"Rusnam","doi":"10.54987/bstr.v6i1.395","DOIUrl":"https://doi.org/10.54987/bstr.v6i1.395","url":null,"abstract":"Quite a few scientific studies carried out on the effectiveness of solvent-extracts from plant as inhibitory agents for microorganisms. A number of these studies, nevertheless, failed to report on the standard IC50 values, that would permit assessment along with other studies. Probably the most correct methods to calculate this value is via analysis of the data using nonlinear regression analysis, in which the four-parameter logistics (4PL) equation is regularly utilized by numerous scientists. In a previous study carried out from solvent extracts from Salvia officinalis, the extract was able to inhibit the bacterium Aeromonas hydrophila, a well-known fish pathogen. However, the study did not report the IC50 value for the extract. Thus, the aim of this research is to apply the four-parameter logistics equation to determine the IC50 value and its confidence interval for the plant extract. The model predicts an IC50 value of 21.92 (mg/ml) with a 95% confidence interval from 20.86 to 23.03, with a correlation coefficient value of 0.96. The result from this study allow future comparative studies to be made with other plant extracts or with other antibacterial activity using this plant extract.","PeriodicalId":436607,"journal":{"name":"Bioremediation Science and Technology Research","volume":"116 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129748004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation of Endophytic Bacteria and Phytoremediation of Soil Contaminated with Polycyclic Aromatic Hydrocarbons Using Cajanus cajan and Lablab purpereus","authors":"M. L. Riskuwa-Shehu, H. Ismail","doi":"10.54987/bstr.v6i1.396","DOIUrl":"https://doi.org/10.54987/bstr.v6i1.396","url":null,"abstract":"Polycyclic aromatic hydrocarbons (PAHs) are among the major compounds posing environmental and health problems worldwide. In the present study, phytoremediation of PAHs using Cajanus cajan and Lablab purpereus in addition to isolation of endophytic bacteria associated with the plant tissues was undertaken. Soil samples contaminated with PAHs were collected from a mechanic workshop in Sokoto metropolis and analysed using standard laboratory procedures. Seeds of the two plants species were sown in the contaminated soil and irrigated for eight weeks to determine the plants’ ability to remediate PAHs. Bacterial count revealed that the plants’ tissues contained 2.3×104 cfu/g and 2.7×104 cfu/g of endophytes. The endophytes were identified to be the members of Pseudomonas, Micrococcus, Bacillus, Rhodococcus and Flavobacterium. GC-MS analysis revealed that the soil samples contained 19.21 ppm PAHs, which were reduced to 2.34 ppm (12.18%) and 4.88 ppm (25.40%) in soils treated with C. cajan and L. purpureus, respectively. Naphthalene was completely degraded in both cases, whereas pyrene, flourene and flouranthene were either completely degraded or significantly reduced. Only indeno (1,2,3 cd) pyrene was least degraded with more than 50% residual concentration. Therefore, the plant species was considered as an important tool in the remediation of PAHs contaminated soil and the role of their endophytes in degradation was thoroughly investigated.","PeriodicalId":436607,"journal":{"name":"Bioremediation Science and Technology Research","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124489465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}