Bacterial Inhibition Activity of Methanolic Extract from Salvia officinalis: Determination of the IC50 value by Nonlinear Regression

Bioremediation Science and Technology Research Pub Date : 2018-07-31 DOI:10.54987/bstr.v6i1.395

Rusnam

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引用次数: 2

Abstract

Quite a few scientific studies carried out on the effectiveness of solvent-extracts from plant as inhibitory agents for microorganisms. A number of these studies, nevertheless, failed to report on the standard IC50 values, that would permit assessment along with other studies. Probably the most correct methods to calculate this value is via analysis of the data using nonlinear regression analysis, in which the four-parameter logistics (4PL) equation is regularly utilized by numerous scientists. In a previous study carried out from solvent extracts from Salvia officinalis, the extract was able to inhibit the bacterium Aeromonas hydrophila, a well-known fish pathogen. However, the study did not report the IC50 value for the extract. Thus, the aim of this research is to apply the four-parameter logistics equation to determine the IC50 value and its confidence interval for the plant extract. The model predicts an IC50 value of 21.92 (mg/ml) with a 95% confidence interval from 20.86 to 23.03, with a correlation coefficient value of 0.96. The result from this study allow future comparative studies to be made with other plant extracts or with other antibacterial activity using this plant extract.

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丹参甲醇提取物抑菌活性:非线性回归法测定IC50值

对植物溶剂提取物作为微生物抑菌剂的有效性进行了大量的科学研究。然而，其中一些研究没有报告可与其他研究一起进行评估的标准IC50值。也许计算这个值的最正确的方法是通过使用非线性回归分析对数据进行分析，其中四参数物流(4PL)方程被许多科学家经常使用。在之前的一项研究中，从鼠尾草中提取的溶剂提取物能够抑制嗜水气单胞菌，一种众所周知的鱼类病原体。然而，该研究没有报告提取物的IC50值。因此，本研究的目的是应用四参数物流方程来确定植物提取物的IC50值及其置信区间。该模型预测IC50值为21.92 (mg/ml)， 95%置信区间为20.86 ~ 23.03，相关系数为0.96。这项研究的结果为将来与其他植物提取物或使用该植物提取物的其他抗菌活性进行比较研究提供了条件。

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Bioremediation Science and Technology Research

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