Bioremediation Science and Technology Research最新文献_第10页

Partial purification of the molybdenum-reducing enzyme from Bacillus pumilus strain Lbna 短小芽孢杆菌Lbna中钼还原酶的部分纯化

Bioremediation Science and Technology Research Pub Date : 2016-07-31 DOI: 10.54987/bstr.v4i1.366

Lubna Kamil Abdulhussein Abo-Shakeer, M. H. Yakasai, M. F. Rahman, N. A. Bakar, M. Syed, N. Abdullah, A. Othman

引用次数: 0

Partial purification and characterization of the molybdenum-reducing enzyme from the glyphosate-degrading Burkholderia vietnamiensis strain AQ5-12 降解草甘膦的越南伯克霍尔德菌AQ5-12中钼还原酶的部分纯化及特性研究

Bioremediation Science and Technology Research Pub Date : 2016-07-31 DOI: 10.54987/bstr.v4i1.363

Siti Nur Khadijah Jamulidin, M. Manogaran, M. H. Yakasai, M. F. Rahman, M. Shukor

引用次数: 1

Phenol removal via cellular immobilization: A mini review 通过细胞固定化去除苯酚:一个小回顾

Bioremediation Science and Technology Research Pub Date : 2016-07-31 DOI: 10.54987/bstr.v4i1.362

Abubakar Aisami, Allamin Ibrahim Alkali, N. A. Yasid

引用次数: 0

Bartlett and the Leveneâ€™s tests of homoscedasticity of the modified Gompertz model used in fitting of Burkholderia sp. strain Neni-11 growth on acrylamide Bartlett和levenez对改良Gompertz模型的均方差检验，用于拟合伯克霍尔德氏菌菌株Neni-11在丙烯酰胺上的生长

Bioremediation Science and Technology Research Pub Date : 2016-07-31 DOI: 10.54987/bstr.v4i1.367

A. Shukor, M. Yunus

引用次数: 5

A rapid inhibitive enzyme assay for monitoring heavy metals pollution in the Juru Industrial Estate 聚如工业区重金属污染的快速抑制酶测定

Bioremediation Science and Technology Research Pub Date : 2015-12-15 DOI: 10.54987/bstr.v3i2.290

M. Halmi, B. Gunasekaran, A. Othman, K. Kamaruddin, F. Dahalan, N. Ibrahim, M. Shukor

{"title":"A rapid inhibitive enzyme assay for monitoring heavy metals pollution in the Juru Industrial Estate","authors":"M. Halmi, B. Gunasekaran, A. Othman, K. Kamaruddin, F. Dahalan, N. Ibrahim, M. Shukor","doi":"10.54987/bstr.v3i2.290","DOIUrl":"https://doi.org/10.54987/bstr.v3i2.290","url":null,"abstract":"The volume of contaminated rivers in Malaysia continues to keep rising through the years. The cost of instrumental monitoring is uneconomical and prohibits schedule monitoring of contaminants particularly heavy metals. In this work, a rapid enzyme assay utilizing the molybdenum-reducing enzyme as an inhibitive assay, prepared in crude form from the molybdenum-reducing bacterium Serratia sp. strain DRY5 has been developed for monitoring the heavy metals mercury, silver, copper and chromium in contaminated waters in the Juru Industrial Estate. The crude enzyme extract transformed soluble molybdenum (phosphomolybdate) into a deep blue solution, which is inhibited by heavy metals such as mercury, silver, copper and chromium. The IC50 and Limits of Detection (LOD) values for mercury, copper, silver and cadmium were 0.245, 0.298, 0.367, 0.326, and 0.124, 0.086, 0.088 and 0.094 mg L-1, respectively. The assay is rapid, and can be carried out in less than 10 minutes. In addition, the assay can be carried out at ambient temperature. The IC50 values for these heavy metals are more sensitive than several established assays. Water samples from various locations in the month of November from the Juru Industrial Estate (Penang) were tested for the presence of heavy metals using the developed assay. Enzyme activity was nearly inhibited for water samples from several locations. The presence of heavy metals was confirmed instrumentally using Atomic Emission Spectrometry and a Flow Injection Mercury System. The assay is rapid and simple and can be used as a first screening method for large scale monitoring of heavy metals.","PeriodicalId":436607,"journal":{"name":"Bioremediation Science and Technology Research","volume":"98 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114695878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Observation of the bile canaliculi of Puntius javanicus liver affected by copper 铜对黄颡鱼肝脏胆小管影响的观察

Bioremediation Science and Technology Research Pub Date : 2015-12-15 DOI: 10.54987/bstr.v3i2.298

M. K. Sabullah, M. Shukor, Azlan Jualang Gansau, B. Gunasekaran, N. A. Shamaan, M. R. Sulaiman

引用次数: 0

Isolation and characterization of a molybdenum-reducing and orange G-decolorizing Enterobacter sp. strain Zeid-6 in soils from Sudan 苏丹土壤中一株钼还原橙色g脱色肠杆菌Zeid-6的分离与特性研究

Bioremediation Science and Technology Research Pub Date : 2015-12-15 DOI: 10.54987/bstr.v3i2.295

A. Othman, I. Zeid, M. F. Rahman, Fazilah Ariffin, M. Shukor

{"title":"Isolation and characterization of a molybdenum-reducing and orange G-decolorizing Enterobacter sp. strain Zeid-6 in soils from Sudan","authors":"A. Othman, I. Zeid, M. F. Rahman, Fazilah Ariffin, M. Shukor","doi":"10.54987/bstr.v3i2.295","DOIUrl":"https://doi.org/10.54987/bstr.v3i2.295","url":null,"abstract":"Chemical toxins and organic contaminants such as hydrocarbons and dyes are major global contaminants with countless tones of those chemicals are created yearly with a significant amount release to the environment. In this work we screen the ability of a molybdenum-reducing bacterium isolated from contaminated soil to decolorize various azo and triphenyl methane dyes independent of molybdenum reduction. Biochemical analysis resulted in a tentative identification of the bacterium as Enterobacter sp. strain Zeid-6. The bacterium was able to decolorize the azo dye Orange G. The bacterium reduces molybdate to Mo-blue optimally at pH between 5.5 and 8.0 and temperatures of between 30 and 37 oC. Other requirements include a phosphate concentration of 5 mM and a molybdate concentration of 20 mM. The absorption spectrum of the Mo-blue produced was similar to previous Mo-reducing bacterium, and closely resembles a reduced phosphomolybdate. Molybdenum reduction was inhibited by copper, lead, mercury and silver which showed 36.8, 16.9, 64.9 and 67.6% inhibition to Mo-reducing activity of Enterobacter sp. strain Zeid-6, respectively. The resultant molybdenum blue spectrum closely resembles the spectrum of molybdenum blue from the phosphate determination method. The ability of this bacterium to detoxify molybdenum and decolorize azo dye makes this bacterium an important tool for bioremediation.","PeriodicalId":436607,"journal":{"name":"Bioremediation Science and Technology Research","volume":"221 5177 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129191926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Bacillus thuringiensis subsp. Entomocidus HD9 Cry1Ba4 insecticidal crystal protein: In-silico mutation, cloning, expression, mutation and domain I functional study 苏云金芽孢杆菌亚种虫虫HD9 Cry1Ba4杀虫晶体蛋白:硅突变、克隆、表达、突变及结构域I功能研究

Bioremediation Science and Technology Research Pub Date : 2015-12-15 DOI: 10.54987/bstr.v3i2.296

Mohd Fadzli Ahmad, H. Abdullah

引用次数: 0

A one dimensional PAGE studies on Puntius javanicus liver proteome affected by copper toxicity 铜中毒对黄颡鱼肝脏蛋白质组的影响

Bioremediation Science and Technology Research Pub Date : 2015-12-15 DOI: 10.54987/bstr.v3i2.297

M. K. Sabullah, Fisal Ahmad, Azlan Jualang Gansau, M. R. Sulaiman

引用次数: 0

Mathematical modeling of molybdenum blue production from Serratia marcescens strain DR.Y10 粘质沙雷氏菌DR.Y10产钼蓝的数学模型

Bioremediation Science and Technology Research Pub Date : 2015-12-15 DOI: 10.54987/bstr.v3i2.289

A. Othman, W. Johari, F. Dahalan, M. Shukor

{"title":"Mathematical modeling of molybdenum blue production from Serratia marcescens strain DR.Y10","authors":"A. Othman, W. Johari, F. Dahalan, M. Shukor","doi":"10.54987/bstr.v3i2.289","DOIUrl":"https://doi.org/10.54987/bstr.v3i2.289","url":null,"abstract":"The pollution of heavy metals and toxic xenobiotics has become a central issue worldwide. Bioremediation of these toxicants are being constantly carried out using novel microbes. Molybdenum reduction to molybdenum blue is a detoxification process and mathematical modelling of the reduction process can reveal important parameters such as specific reduction rate, theoretical maximum reduction and whether reduction at high molybdenum concentration affected the lag period of reduction. The used of linearization method through the use of natural logarithm transformation, although popular, is inaccurate and can only give an approximate value for the sole parameter measured; the specific growth rate. In this work, a variety of models for such as logistic, Gompertz, Richards, Schnute, Baranyi-Roberts, Von Bertalanffy, Buchanan three-phase and more recently Huang were utilized for the first time to obtain values for the above parameters or constants. The modified Gompertz model was the best model in modelling the Mo-blue production curve from Serratia marcescens strain DR.Y10 based on statistical tests such as root-mean-square error (RMSE), adjusted coefficient of determination (R2), bias factor (BF), accuracy factor (AF) and corrected AICc (Akaike Information Criterion). Parameters obtained from the fitting exercise were maximum Mo-blue production rate (um), lag time (l) and maximal Mo-blue production (Ymax) of X (h-1), Y (h) and Z (nmole Mo-blue), respectively. The application of primary population growth models in modelling the Mo-blue production rate from this bacterium has become a successful undertaking. The model may also be used in other heavy metals detoxification processes. The parameters constants extracted from this work will be a substantial help for the future development of further secondary models.","PeriodicalId":436607,"journal":{"name":"Bioremediation Science and Technology Research","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126998603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4