Bioremediation Science and Technology Research最新文献_第9页

Kinetic studies of the partially purified molybdenum-reducing enzyme from Bacillus pumilus strain lbna 短小芽孢杆菌lbna部分纯化钼还原酶的动力学研究

Bioremediation Science and Technology Research Pub Date : 2017-07-31 DOI: 10.54987/bstr.v5i1.354

Lubna Kamil Abdulhussein Abo-Shakeer, M. F. Rahman, M. H. Yakasai, N. A. Bakar, A. Othman, M. Syed, N. Abdullah, M. Shukor

{"title":"Kinetic studies of the partially purified molybdenum-reducing enzyme from Bacillus pumilus strain lbna","authors":"Lubna Kamil Abdulhussein Abo-Shakeer, M. F. Rahman, M. H. Yakasai, N. A. Bakar, A. Othman, M. Syed, N. Abdullah, M. Shukor","doi":"10.54987/bstr.v5i1.354","DOIUrl":"https://doi.org/10.54987/bstr.v5i1.354","url":null,"abstract":"Bacterial based remediation of environmental toxicants is a promising innovative technology for molybdenum pollution. To date, the enzyme responsible for molybdate reduction to Mo-blue from bacteria show that the Michaelis-Menten constants varies by one order of magnitude. It is important that the constants from newer enzyme sources be characterized so that a comparison can be made. The aim of this study is to characterize kinetically the enzyme from a previously isolated Mo-reducing bacterium; Bacillus pumilus strain Lbna. The maximum activity of this enzyme occurred at pH 5.5 and in between 25 and 35 oC. The Km and Vmax of NADH were 6.646 mM and 0.057 unit/mg enzyme, while the Km and Vmax of LPPM were 3.399 mM and 0.106 unit/mg enzyme. The results showed that the enzyme activity for Bacillus pumilus strain Lbna were inhibited by all heavy metals used. Zinc, copper, silver, chromium, cadmium and mercury all caused more than 50% inhibition to the Mo-reducing enzyme activity with copper being the most potent with an almost complete inhibition of enzyme activity observed.","PeriodicalId":436607,"journal":{"name":"Bioremediation Science and Technology Research","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130038175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

The development of banana peel/corn starch bioplastic film: a preliminary study 香蕉皮/玉米淀粉生物塑料薄膜的初步研究

Bioremediation Science and Technology Research Pub Date : 2017-07-31 DOI: 10.54987/bstr.v5i1.352

N. Sultan, W. Johari

{"title":"The development of banana peel/corn starch bioplastic film: a preliminary study","authors":"N. Sultan, W. Johari","doi":"10.54987/bstr.v5i1.352","DOIUrl":"https://doi.org/10.54987/bstr.v5i1.352","url":null,"abstract":"The aim of this study is to develop bioplastic film from a combination of two biopolymers of same source, namely banana peel and corn starch. Five banana peel films (BP film) were prepared with different concentrations of corn starch (1% up to 5%) as co-biopolymer and film without corn starch acted as a control. The films were carried out with several durability tests and characterization analyses. Based on the results obtained, the BP film with 4% corn starch gave the highest tensile strength 34.72 N/m2 compared to other samples. The water absorption test showed that BP films with 3% corn starch were resistant to water uptake by absorbing water up to 60.65%. In terms of characterization, spectra of Fourier Transform Infrared Spectroscopy (FTIR) obtained for BP control film and BP film with 4% corn starch were comparable with most of the peaks were present. The thermal analysis by differential screening calorimetric (DSC) detected the melting temperature for both BP control film and BP film with 4% corn respectively at Tonset of 54.41Â°C and 67.83Â°C. Overall, combination of starches from two different sources can be used as an alternative in producing bioplastics.","PeriodicalId":436607,"journal":{"name":"Bioremediation Science and Technology Research","volume":"337 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124730781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 47

Production of rhamnolipids by locally isolated Pseudomonas aeruginosa using sunflower oil as carbon source 以葵花籽油为碳源的本地分离铜绿假单胞菌生产鼠李糖脂

Bioremediation Science and Technology Research Pub Date : 2017-07-31 DOI: 10.54987/bstr.v5i1.350

Helmi Wasoh, Sarinah Baharun, M. Halim, A. Lajis, A. Ariff, O. Lai

{"title":"Production of rhamnolipids by locally isolated Pseudomonas aeruginosa using sunflower oil as carbon source","authors":"Helmi Wasoh, Sarinah Baharun, M. Halim, A. Lajis, A. Ariff, O. Lai","doi":"10.54987/bstr.v5i1.350","DOIUrl":"https://doi.org/10.54987/bstr.v5i1.350","url":null,"abstract":"Biosurfactants are surface active compounds and amphiphatic in nature which consist of hydrophilic head and hydrophobic tail accumulating at the interphase of two immiscible liquid with different polarity. A study was conducted to investigate the effectiveness of sunflower oil in the production of rhamnolipids (RLs) by locally isolated Pseudomonas aeruginosa in shake flask fermentation. In this process, four different fermentation treatments were done for seven days at 30Â°C and 180 rpm. Sampling was carried out in time intervals of 24 h followed by monitoring of cell growth and biosurfactants production. Colorimetric Orcinol analysis was used for determination of RLs concentrations (g/L). The RLs were studied for emulsification activity using emulsification index (E24%) methods. In addition, oil displacement activity and thermal stability were also studied (4-120Â°C). All treatments allow the growth of P. aeruginosa and the utilization of sunflower oil as carbon source and glucose as growth initiator were observed to be the best strategy for maximum RLs production. The maximum RLs production was achieved after 120 h with 3.18 g/L of RLs. Diesel shows the highest emulsification activity among the substrate tested ranging from 55.56% - 60.00%. The oil displacement activity was corresponding to RLs concentration with stability up to 120Â°C (for 60 min). Therefore, from this research a good potential of RLs that may provide good application for industry were produced.","PeriodicalId":436607,"journal":{"name":"Bioremediation Science and Technology Research","volume":"6 5","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120815156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Assessment of acetylcholinesterase (AChE) from Oreochromis mossambicus (Cuvier, 1831) as a source of enzyme for insecticides detection mosambicus (Cuvier, 1831)中乙酰胆碱酯酶(AChE)作为杀虫剂检测酶源的评价

Bioremediation Science and Technology Research Pub Date : 2016-12-31 DOI: 10.54987/bstr.v4i2.370

Umar Abubakar Muhammad, L. G. Tham, N. Perumal, H. Daud, N. A. Yasid, M. Shukor

{"title":"Assessment of acetylcholinesterase (AChE) from Oreochromis mossambicus (Cuvier, 1831) as a source of enzyme for insecticides detection","authors":"Umar Abubakar Muhammad, L. G. Tham, N. Perumal, H. Daud, N. A. Yasid, M. Shukor","doi":"10.54987/bstr.v4i2.370","DOIUrl":"https://doi.org/10.54987/bstr.v4i2.370","url":null,"abstract":"In this work we assess the potential of acetylcholinesterase (AChE) from Oreochromis mossambicus (Toman) as a sensitive test for the presence of insecticides. The partial purification and characterization of a soluble AChE from Oreochromis mossambicus brain tissues using affinity chromatography gel (procainamideâ€“Sephacryl S-1000) showed that the partially purified AChE was most active on acetylthiocholine (ATC) but had low activities on propionylthiocholine (PTC) and butyrylthiocholine (BTC), indicating that the partially purified fraction was predominantly AChE. Soluble AChE was partially purified 9.27-fold with a 91.12% yield. The partially purified AChE displayed the highest activity on ATC at pH 7 and at 30oC using 0.1 M Tris buffer. The enzyme exhibited Michaelis-Menten kinetic constants, Km, for ATC, BTC and PTC at 36, 77 and 250 ÂµM, respectively, and the maximum velocities, Vmax, were 18.75, 0.12 and 0.05 Âµmol/min/mg protein, respectively. Moreover, the AChE from Oreochromis mossambicus presented comparable sensitivity to carbamates and organophosphates insecticides than that from Electrophorus electricus and many other fish AChE by comparing half maximal inhibitory concentration values. Therefore, the enzyme is a valuable source for insecticides detection in Malaysian waters at lower cost. ","PeriodicalId":436607,"journal":{"name":"Bioremediation Science and Technology Research","volume":"50 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123500178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Mathematical modelling of molybdenum reduction to mo-blue by a cyanide-degrading bacterium 一种氰化物降解细菌将钼还原为钼蓝的数学模型

Bioremediation Science and Technology Research Pub Date : 2016-12-31 DOI: 10.54987/bstr.v4i2.368

M. H. Yakasai, K. Ibrahim, N. A. Yasid, M. Halmi, M. F. Rahman, M. Shukor

{"title":"Mathematical modelling of molybdenum reduction to mo-blue by a cyanide-degrading bacterium","authors":"M. H. Yakasai, K. Ibrahim, N. A. Yasid, M. Halmi, M. F. Rahman, M. Shukor","doi":"10.54987/bstr.v4i2.368","DOIUrl":"https://doi.org/10.54987/bstr.v4i2.368","url":null,"abstract":"Molybdenum, an emerging pollutant, has being demonstrated recently to be toxic to spermatogenesis in several animal model systems. Metal mines especially gold mine often use cyanide and hence isolation of metal-reducing and cyanide-degrading bacteria can be useful for the bioremediation of these pollutants. Preliminary screening shows that three cyanide-degrading bacteria were able to reduce molybdenum to molybdenum blue (Mo-blue) when grown on a molybdate low phosphate minimal salts media. Phylogenetic analyses of the 16S rRNA gene of the best reducer indicates that it belongs to the Serratia genus. A variety of mathematical models such as logistic, Gompertz, Richards, Schnute, Baranyi-Roberts, von Bertalanffy, Buchanan three-phase and Huang were used to model molybdenum reduction, and the best model based on statistical analysis was modified Gompertz with lowest values for RMSE and AICc, highest adjusted R2 values, with Bias Factor and Accuracy Factor nearest to unity (1.0). The reduction constants obtained from the model will be used to carry out secondary modelling to study the effect of various parameters such as substrate, pH and temperature to molybdenum reduction.","PeriodicalId":436607,"journal":{"name":"Bioremediation Science and Technology Research","volume":"82 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116106473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Isolation and characterization of a 2,4-dinitrophenol-degrading bacterium 2,4-二硝基苯酚降解菌的分离与鉴定

Bioremediation Science and Technology Research Pub Date : 2016-12-31 DOI: 10.54987/bstr.v4i2.373

Noor Suffiah Md. Zin, S. N. Padrilah, M. F. Rahman, H. K. Sim, A. Khalid, M. Shukor

引用次数: 1

Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Clarias batrachus 用一种基于来自batrachus的乙酰胆碱酯酶的抑制测定法测定重金属

Bioremediation Science and Technology Research Pub Date : 2016-12-31 DOI: 10.54987/bstr.v4i2.369

Umar Abubakar Muhammad, L. G. Tham, N. Perumal, H. Daud, N. A. Yasid, M. Shukor

引用次数: 2

Test for the presence of autocorrelation in the modified Gompertz model used in fitting of Burkholderia sp. strain Neni-11 growth on acrylamide 修正Gompertz模型拟合伯克霍尔德氏菌菌株Neni-11在丙烯酰胺上生长的自相关性检验

Bioremediation Science and Technology Research Pub Date : 2016-12-31 DOI: 10.54987/bstr.v4i2.372

A. Shukor, M. Yunus

引用次数: 0

Characterization of a molybdenum-reducing Acinetobacter baumannii strain Serdang 1 with the capacity to grow on phenol and acrylamide 一株能在苯酚和丙烯酰胺上生长的降钼鲍曼不动杆菌Serdang 1的特性

Bioremediation Science and Technology Research Pub Date : 2016-12-31 DOI: 10.54987/bstr.v4i2.371

Z. H. M. Yadzir, Aa'ishah Abd Gafar, M. H. Yakasai, M. F. Rahman, M. A. Abdullah, N. A. Shamaan, M. Halmi

{"title":"Characterization of a molybdenum-reducing Acinetobacter baumannii strain Serdang 1 with the capacity to grow on phenol and acrylamide","authors":"Z. H. M. Yadzir, Aa'ishah Abd Gafar, M. H. Yakasai, M. F. Rahman, M. A. Abdullah, N. A. Shamaan, M. Halmi","doi":"10.54987/bstr.v4i2.371","DOIUrl":"https://doi.org/10.54987/bstr.v4i2.371","url":null,"abstract":"Contamination of organic xenobiotic pollutants and heavy metals in a contaminated site allows the use of multiple bacterial degraders or bacteria with the ability to detoxify numerous toxicants at the same time. A previously isolated SDS- degrading bacterium, Acinetobacter baumannii strain Serdang 1 was shown to reduce molybdenum to molybdenum-blue. The bacterium works optimally at pH 6.5, the temperature range between 25 and 34Â°C with glucose serves as the best electron donor for molybdate reduction. This bacterium required additional concentration of phosphate at 5.0 mM and molybdate between 15 and 25 mM. The absorption spectrum of the molybdenum blue obtained is similar to the molybdenum blue from other earlier reported molybdate reducing bacteria, as it resembles a reduced phosphomolybdate closely. Ag(i), As(v), Pb(ii) and Cu(ii) inhibited molybdenum reduction by 57.3, 36.8, 27.7 and 10.9%, respectively, at 1 p.p.m. Acrylamide was efficiently shown to support molybdenum reduction at a lower efficiency than glucose. Phenol, acrylamide and propionamide could support the growth of this bacterium independently of molybdenum reduction. This bacterium capability to detoxify several toxicants is an important tool for bioremediation in the tropical region","PeriodicalId":436607,"journal":{"name":"Bioremediation Science and Technology Research","volume":"129 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121526138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Near real-time biomonitoring of copper from an industrial complex effluent discharge site using a plant protease inhibitive assay 利用植物蛋白酶抑制试验对某工业综合体废水排放现场的铜进行近实时生物监测

Bioremediation Science and Technology Research Pub Date : 2016-07-31 DOI: 10.54987/bstr.v4i1.365

M. Halmi, M. E. Khayat, B. Gunasekaran, N. A. Masdor, M. F. Rahman

引用次数: 7