Bioremediation Science and Technology Research最新文献_第8页

Isolation and Characterization of a Molybdenum-reducing and Methylene Blue-decolorizing Serratia marcescens strain KIK-1 in Soils from Nigeria 尼日利亚土壤中还原钼亚甲基蓝脱色粘质沙雷氏菌KIK-1的分离与鉴定

Bioremediation Science and Technology Research Pub Date : 2018-07-31 DOI: 10.54987/bstr.v6i1.392

Ibrahim Kabiru Karamba, H. Yakasai

{"title":"Isolation and Characterization of a Molybdenum-reducing and Methylene Blue-decolorizing Serratia marcescens strain KIK-1 in Soils from Nigeria","authors":"Ibrahim Kabiru Karamba, H. Yakasai","doi":"10.54987/bstr.v6i1.392","DOIUrl":"https://doi.org/10.54987/bstr.v6i1.392","url":null,"abstract":"Bioremediation of toxic compound in polluted environment is currently considered as the most economical and ecofriendly approach, particularly at a lower concentration of the toxicant, where other physicochemical techniques are ineffective. In this work, molybdenum-reducing bacterium with the capacity to decolorize various azo and triphenyl methane dyes independent of molybdenum reduction was isolated from contaminated soil. The bacterium reduces molybdate to Mo-blue optimally at pH between 5.8 and 6.5, temperature, between 34 and 37 oC, molybdate concentration between 10 and 25 mM and phosphate concentration, 5.0 mM. Glucose was the best electron donor supporting molybdate reduction followed by sucrose, maltose, trehalose, d-mannose, glycerol, d-mannitol, d-sorbitol, myo-inositol, d-adonitol and salicin in descending order. The absorption spectrum of Mo-blue produced was similar to other previous Mo-reducing bacteria, and closely resembles a reduced phosphomolybdate. About 78.1, 63.4, 45.5 and 17.8% of the molybdenum reduction in this bacterium was inhibited by 2 ppm mercury (ii), silver (i), copper (ii) and chromium (vi), respectively. The biochemical analysis resulted in a tentative identification of the bacterium as Serratia marcescens strain KIK-1. The ability of this bacterium to detoxify molybdenum and decolorize azo dye makes this bacterium an important tool for bioremediation. \u0000 \u0000 ","PeriodicalId":436607,"journal":{"name":"Bioremediation Science and Technology Research","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132023131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Preliminary Screening of Plant Proteases as a Potential Source for the Development of an Inhibitive Assay for Heavy Metals 植物蛋白酶作为开发重金属抑制试验的潜在来源的初步筛选

Bioremediation Science and Technology Research Pub Date : 2018-07-31 DOI: 10.54987/bstr.v6i1.393

B. Gunasekaran, W. Johari, Helmi Wasoh, N. A. Masdor, M. Shukor

引用次数: 1

Isolation and culture medium optimisation using one-factor-at-time and Response Surface Methodology on the biodegradation of the azo-dye amaranth 偶氮染料苋菜生物降解的单因子法和响应面法分离及培养基优化

Bioremediation Science and Technology Research Pub Date : 2017-12-31 DOI: 10.54987/bstr.v5i2.360

Noris Kartika Sari Islahuddin, M. Halmi, M. Manogaran, M. Shukor

{"title":"Isolation and culture medium optimisation using one-factor-at-time and Response Surface Methodology on the biodegradation of the azo-dye amaranth","authors":"Noris Kartika Sari Islahuddin, M. Halmi, M. Manogaran, M. Shukor","doi":"10.54987/bstr.v5i2.360","DOIUrl":"https://doi.org/10.54987/bstr.v5i2.360","url":null,"abstract":"Isolate JR1 was isolated from the polluted textile industry activities site in the Juru Penang area. This bacterium was characterized as a gram-positive Bacillus bacterium and also gave a positive biochemical test for catalase test and oxidase test. The isolate JR1 gave a maximum decolourization of Amaranth dye under static conditions with the rate of decolorization of 98.82%. Seven variables which are pH, temperature (Â°C), ammonium acetate (g/L), glucose (g/L), sodium chloride (g/L), yeast (g/L) and dye concentration (ppm) was run by using Plackett-Burman design for the effective parameter of the decolourization of Amaranth. From the seven variables, three effective variables which were ammonium acetate, glucose, and dye concentration were further optimized by using a central composite design. The optimum value of ammonium acetate concentration at 0.74 g/L, glucose concentration at 3.0 g/L and a dye concentration at 58.1 ppm gave the highest percentage of decolourization. Thus, this isolate could provide an alternate solution in removing toxic dyes from environments.","PeriodicalId":436607,"journal":{"name":"Bioremediation Science and Technology Research","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130885547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Screening of hydrocarbon-degrading bacterial isolates using the redox application of 2,6-DCPIP 2,6- dcpip氧化还原应用筛选烃类降解细菌

Bioremediation Science and Technology Research Pub Date : 2017-12-31 DOI: 10.54987/bstr.v5i2.358

Nur Muhamad Syahir Abdul Habib, W. Johari, M. Shukor, N. A. Yasid

引用次数: 14

Isolation and characterisation of a Mo-reducing bacterium from Malaysian soil 马来西亚土壤中一种钼还原菌的分离与鉴定

Bioremediation Science and Technology Research Pub Date : 2017-12-31 DOI: 10.54987/bstr.v5i2.359

Hui Sin Chee, M. Manogaran, Z. Suhaili, M. H. Yakasai, M. F. Rahman, N. A. Shamaan, N. A. Yasid, A. Othman

{"title":"Isolation and characterisation of a Mo-reducing bacterium from Malaysian soil","authors":"Hui Sin Chee, M. Manogaran, Z. Suhaili, M. H. Yakasai, M. F. Rahman, N. A. Shamaan, N. A. Yasid, A. Othman","doi":"10.54987/bstr.v5i2.359","DOIUrl":"https://doi.org/10.54987/bstr.v5i2.359","url":null,"abstract":"The issue of heavy metal contamination and toxic xenobiotics has become a rapid global concern. This has ensured that the bioremediation of these toxicants, which are being carried out using novel microbes. A bacterium with the ability to reduce molybdenum has been isolated from contaminated soils and identified as Serratia marcescens strain DR.Y10. The bacterium reduced molybdenum (sodium molybdate) to molybdenum blue (Mo-blue) optimally at pHs of between 6.0 and 6.5 and temperatures between 30Â°C and 37Â°C. Glucose was the best electron donor for supporting molybdate reduction followed by sucrose, adonitol, mannose, maltose, mannitol glycerol, salicin, myo-inositol, sorbitol and trehalose in descending order. Other requirements include a phosphate concentration of 5 mM and a molybdate concentration of between 10 and 30 mM. The absorption spectrum of the Mo-blue produced was similar to the previously isolated Mo-reducing bacterium and closely resembles a reduced phosphomolybdate. Molybdenum reduction was inhibited by Hg (ii), Ag (i), Cu (ii), and Cr (vi) at 78.9, 69.2, 59.5 and 40.1%, respectively. We also screen for the ability of the bacterium to use various organic xenobiotics such as phenol, acrylamide, nicotinamide, acetamide, iodoacetamide, propionamide, acetamide, sodium dodecyl sulfate (SDS) and diesel as electron donor sources for aiding reduction. The bacterium was also able to grow using amides such as acrylamide, propionamide and acetamide without molybdenum reduction. The unique ability of the bacterium to detoxify many toxicants is much in demand, making this bacterium a vital means of bioremediation.","PeriodicalId":436607,"journal":{"name":"Bioremediation Science and Technology Research","volume":"50 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125038693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Acrylamide toxicity and its biodegradation 丙烯酰胺的毒性及其生物降解

Bioremediation Science and Technology Research Pub Date : 2017-12-31 DOI: 10.54987/bstr.v5i2.357

Aa'ishah Abd Gafar, M. E. Khayat, M. Rahim, M. Shukor

引用次数: 8

Isolation and characterization of a metal-reducing Pseudomonas sp. strain 135 with amide-degrading capability 具有酰胺降解能力的金属还原假单胞菌菌株135的分离与鉴定

Bioremediation Science and Technology Research Pub Date : 2017-12-31 DOI: 10.54987/bstr.v5i2.361

M. H. Yakasai, M. F. Rahman, M. Rahim, M. E. Khayat, N. A. Shamaan, M. Shukor

{"title":"Isolation and characterization of a metal-reducing Pseudomonas sp. strain 135 with amide-degrading capability","authors":"M. H. Yakasai, M. F. Rahman, M. Rahim, M. E. Khayat, N. A. Shamaan, M. Shukor","doi":"10.54987/bstr.v5i2.361","DOIUrl":"https://doi.org/10.54987/bstr.v5i2.361","url":null,"abstract":"The presence of both heavy metals and organic xenobiotic pollutants in a contaminated site justifies the application of either a multitude of microbial degraders or microorganisms having the capacity to detoxify a number of pollutants at the same time. Molybdenum is an essential heavy metal that is toxic to ruminants at a high level. Ruminants such as cow and goats experience severe hypocuprosis leading to scouring and death at a concentration as low as several parts per million. In this study, a molybdenum-reducing bacterium with amide-degrading capacity has been isolated from contaminated soils. The bacterium, using glucose as the best electron donor reduces molybdenum in the form of sodium molybdate to molybdenum blue. The maximal pH reduction occurs between 6.0 and 6.3, and the bacterium showed an excellent reduction in temperatures between 25 and 40 oC. The reduction was maximal at molybdate concentrations of between 15 and 25 mM. Molybdenum reduction incidentally was inhibited by several toxic heavy metals. Other carbon sources including toxic xenobiotics such as amides were screened for their ability to support molybdate reduction. Of all the amides, only acrylamide can support molybdenum reduction. The other amides; such as acetamide and propionamide can support growth. Analysis using phylogenetic analysis resulted in a tentative identification of the bacterium as Pseudomonas sp. strain 135. This bacterium is essential in remediating sites contaminated with molybdenum, especially in agricultural soil co-contaminated with acrylamide, a known soil stabilizer.","PeriodicalId":436607,"journal":{"name":"Bioremediation Science and Technology Research","volume":"89 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115830708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Phenol removal via cellular immobilization: A review 细胞固定化法去除苯酚研究进展

Bioremediation Science and Technology Research Pub Date : 2017-12-31 DOI: 10.54987/bstr.v5i2.356

A. Abubakar, M. Shukor

{"title":"Phenol removal via cellular immobilization: A review","authors":"A. Abubakar, M. Shukor","doi":"10.54987/bstr.v5i2.356","DOIUrl":"https://doi.org/10.54987/bstr.v5i2.356","url":null,"abstract":"Environmental pollution is one of the major concerns in the 21st century; where billions of tonnes of harmful chemicals are produced by industries such as petroleum, paints, food, rubber, and plastic. Phenol and its derivatives infiltrate the ecosystems and has become one of the top major pollutants worldwide. This review covers the major aspects of immobilization of phenol-degrading bacteria as a method to improve phenol bioremediation. The use of various forms of immobilization matrices is discussed along with the advantages and disadvantages of each of the immobilization matrices especially when environmental usage is warranted. To be used as a bioremediation tool, immobilized system must not only be effective, but the matrices must be non-toxic, non-polluting and if possible non-biodegradable. The mechanical, biological and chemical stability of the system is paramount for long term activity as well as price is an important factor when very large scale is concern. The system must also be able to tolerate high concentration of other toxicants especially heavy metals that form as co-contaminants, and most immobilized systems are geared towards this last aspect as immobilization generally provides protection from other contaminants.","PeriodicalId":436607,"journal":{"name":"Bioremediation Science and Technology Research","volume":"383 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132442078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Channa striatus 用一种基于青霉乙酰胆碱酯酶的抑制法测定重金属

Bioremediation Science and Technology Research Pub Date : 2017-07-31 DOI: 10.54987/bstr.v5i1.351

Ain Farihah Zulkifli, L. G. Tham, N. Perumal, M. K. Sabullah, A. M. Azzeme, M. Shukor, N. A. Shaharuddin

引用次数: 3

Estimation of the Q10 value; the temperature coefficient for the growth of Pseudomonas sp. aq5-04 on phenol Q10值的估计;假单胞菌aq5-04对苯酚的生长温度系数

Bioremediation Science and Technology Research Pub Date : 2017-07-31 DOI: 10.54987/bstr.v5i1.355

Abubakar Aisami, N. A. Yasid, W. Johari, M. Shukor

引用次数: 16