2,6- dcpip氧化还原应用筛选烃类降解细菌

Nur Muhamad Syahir Abdul Habib, W. Johari, M. Shukor, N. A. Yasid
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引用次数: 14

摘要

石油碳氢化合物仍然是世界各地可以发现的主要污染物。利用微生物作为生物修复手段的生物修复方法因其突出的价值而得到广泛认可。因此,关于分离和鉴定新的烃类降解菌株的科学报告不断增加。在定性和定量评估中,基于比色法的分析是鉴定烃类降解菌株能力的最快方法之一。本研究通过2,6-二氯酚吲哚酚(DCPIP)试验,观察了9株分离菌对碳氢化合物的降解能力。两个有效的柴油利用分离物显示出独特的倾向,利用芳香(ADL15)和脂肪族(ADL36)碳氢化合物。这两种分离物被证明是广泛的石油烃组分生物修复的良好候选者。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Screening of hydrocarbon-degrading bacterial isolates using the redox application of 2,6-DCPIP
Petroleum hydrocarbons remain as the major contaminants that could be found across the world. Remediation approach through the utilisation of microbes as the bioremediation means is widely recognised due to their outstanding values. As a result, scientific reports on the isolation and identification of new hydrocarbon-degrading strains were on the rise. Colourimetric-based assays are one of the fastest methods to identify the capability of hydrocarbon-degrading strains in both qualitative and quantitative assessment. In this study, the hydrocarbon-degrading potential of nine bacterial isolates was observed via 2,6-dichlorophenolindophenol (DCPIP) test. Two potent diesel-utilising isolates show a distinctive tendency to utilise aromatic (ADL15) and aliphatic (ADL36) hydrocarbons. Both isolates prove to be a good candidate for bioremediation of wide range of petroleum hydrocarbon components.
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