{"title":"Regulatory Processes and Standards Required for Marketing Authorization of Vaccines in Brazil","authors":"Sheila Matsuoka, L. Castanheira, I. Silveira","doi":"10.35259/ISI.SACT.2019_32783","DOIUrl":"https://doi.org/10.35259/ISI.SACT.2019_32783","url":null,"abstract":"","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131133136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Silva, S. Pereira, Natália Pedra Gonçalves, G. M. D. Conceição, L. C. Ferreira, F. Figueiredo, R. Menezes
{"title":"Comparative study of in situ hybridization and quantitative PCR for diagnosis of cutaneous infection by Leishmania infantum in dogs","authors":"R. Silva, S. Pereira, Natália Pedra Gonçalves, G. M. D. Conceição, L. C. Ferreira, F. Figueiredo, R. Menezes","doi":"10.35259/ISI.SACT.2019_ARCA32802","DOIUrl":"https://doi.org/10.35259/ISI.SACT.2019_ARCA32802","url":null,"abstract":"","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"177 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124385622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Institutional policies: how the market prepares, documents and discloses its guidelines","authors":"C. Silva, Francisco Elton Ribeiro","doi":"10.35259/isi.sact.2019_32792","DOIUrl":"https://doi.org/10.35259/isi.sact.2019_32792","url":null,"abstract":"Methodology: For this study, 12 large institutions were analyzed, of which 5 were public. For this purpose, general and specific questionnaires were sent, such as: “What is the current structure or hierarchy of documents established in the Organization?”, “Is there any formalized classification for Institutional Policies as to its scope or comprehensiveness?”, Are there formal criteria for assessing whether the policy is effectively contributing to the company’s overall objective and values?”, “What are the topics covered in Institutional Policies”,”How is the process of establishing Institutional Policies (elaboration, review, approval ), “Is there a document that describes the sanctions and penalties, in case someone does not follow the Institutional Policies?”. And, from the answers, an analysis and diagnosis report was prepared.","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116882283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Rocha, B. F. C. Patricio, H. Rocha, B. Souza, P. C. Neves, N. Yoshihara, B. Archanjo, T. Tilli, A. Bom
{"title":"Development, characterization and validation of interfering RNA carrier liposomes for breast cancer treatment","authors":"A. Rocha, B. F. C. Patricio, H. Rocha, B. Souza, P. C. Neves, N. Yoshihara, B. Archanjo, T. Tilli, A. Bom","doi":"10.35259/ISI.SACT.2019_32654","DOIUrl":"https://doi.org/10.35259/ISI.SACT.2019_32654","url":null,"abstract":"","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115564362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Economic viability in the use of agitated single-use bioreactors as an alternative to rigid systems in a prokaryotic plant","authors":"Miguel O Herrera, Wallace Silva, E. Lemes","doi":"10.35259/ISI.SACT.2019_32759","DOIUrl":"https://doi.org/10.35259/ISI.SACT.2019_32759","url":null,"abstract":"Introduction: Nowadays, agitated single-use bioreactors are prominent in the pharmaceutical industry as potential alternatives to conventional rigid systems. This is due to the advantages associated with this technology. For example, it reduces operating costs due to the lack of cleaning and sterilization. However, in an economic evaluation, it is necessary to emphasize the associated costs of bags, the impact of disposal, the volumetric production capacity and the operational limits of the technology when compared with conventional systems.","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"289 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122691586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Closing the gap in early diagnosis of autoimmune rheumatic diseases: discovery of novel biomarkers using high-density peptide microarrays","authors":"K. Heiss, P. Simonini, Renate Sekul, V. Stadler","doi":"10.35259/ISI.SACT.2019_32761","DOIUrl":"https://doi.org/10.35259/ISI.SACT.2019_32761","url":null,"abstract":"Introduction: Autoimmune rheumatic diseases such as rheumatoid arthritis (RA) and psoriatic arthritis (PsA) are attributed to inflammation affecting the joints and connective tissues, with extra-articular manifestations in some disease types. Early diagnosis is the key to optimal therapeutic success in order to slow down disease progression or even prevent joint damage and hence irreversible disability. However, diagnosis in an early disease stage is often challenging. In RA, up to 30% of early stage patients are negative for the current serological diagnostic measures using antibodies against cyclic citrullinated peptide antigens (ACPA) or rheumatoid factor (RF). In PsA, both RF and ACPA are mainly absent. Moreover, the heterogeneous disease phenotypes associated with PsA often make an early diagnosis difficult, especially in patients without psoriatic skin lesions.","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128885563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. Lago, N. T. Paula, Giovana P. Angelice, Francisco C. A. Mello, V. Paula, M. Lavatori, F. Aragão, E. Lampe, Rs Brasil. Embrapa Pecuária Sul. Bagé
{"title":"Development of lentiviral vectors for inhibition of hepatitis B virus, via small interfering RNAi","authors":"B. Lago, N. T. Paula, Giovana P. Angelice, Francisco C. A. Mello, V. Paula, M. Lavatori, F. Aragão, E. Lampe, Rs Brasil. Embrapa Pecuária Sul. Bagé","doi":"10.35259/isi.sact.2019_32729","DOIUrl":"https://doi.org/10.35259/isi.sact.2019_32729","url":null,"abstract":"Introduction: It is estimated that chronic hepatitis B virus (HBV) infection accounts for approximately one million deaths per year due to severe cirrhosis and hepatocellular carcinoma (HCC). Currently, several drugs are used in the treatment of chronic hepatitis B, however, complete cure is still controversial. The major challenge is the persistence of viral covalently closed circular DNA (cccDNA), as well as the ability of HBV to integrate into the host genome, which enables the infection’s reactivation. Interfering RNA (RNAi) is a post-transcriptional mechanism of gene silencing and demonstrates to be a promising alternative for the treatment of chronic hepatitis B.","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124475819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Physical-chemical methodologies for molecular characterization of carrier proteins used in Bio-Manguinhos conjugated vaccines","authors":"I. Corrêa, R. Bastos, P. Jurgilas, H. Nascimento","doi":"10.35259/isi.sact.2019_32537","DOIUrl":"https://doi.org/10.35259/isi.sact.2019_32537","url":null,"abstract":"Methodology: SEC chromatographic profile of two different samples of TT (TT1 and TT2) were performed using three different columns (Zorbax®GF450, TSK®G4000 SWXL and SuperdexTM 200). Electrophoretic techniques such as SDS-PAGE, IEF-PAGE and NATIVE-PAGE were used to evaluate electrophoretic profile. Stability of secondary and tertiary structures were analyzed by circular dichroism and fluorescence spectroscopy respectively, with temperature varying between 25°C-85°C. All proteins were subjected to tryptic hydrolysis aiming obtain a peptide map by RPC chromatography. TT1 was used as standard for all analysis.","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126864563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Lessons learnt from the Plasmodium vivax MSP1 towards malaria control and elimination efforts","authors":"P. Nogueira, Elizangela Farias","doi":"10.35259/isi.sact.2019_32722","DOIUrl":"https://doi.org/10.35259/isi.sact.2019_32722","url":null,"abstract":"Methodology: The quantification and genotyping of parasite based on MSP1 haplotypes using the new generation sequencing and comparison with a haplotype-specific serology against a broad set of Block 2 PvMSP1 peptides. The humoral response based on isotype, subclass and avidity profiles against a set of recombinant proteins PvMSP1, such as MSP1-19, N-terminal PvMSP1 and set of Block 2 PvMSP1 variants.","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"50 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126132823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. L. Kessler, Alexandre Haruo Inoue, Henrique Preti, M. Serpeloni, M. V. Alcantara, F. Marchini, M. Krieger
{"title":"Genomic integration as a way to stable high producers CHO cells","authors":"R. L. Kessler, Alexandre Haruo Inoue, Henrique Preti, M. Serpeloni, M. V. Alcantara, F. Marchini, M. Krieger","doi":"10.35259/isi.sact.2019_32710","DOIUrl":"https://doi.org/10.35259/isi.sact.2019_32710","url":null,"abstract":"Methodology: This methodology is based on DNA genomic integration by sleeping beauty transposase. Transfections were made with 25 μg of PEI (polyethylenimine) and 12,5 μg of total DNA (75% of DNA of interest and 25% of transposase DNA) for a total of 107 cells. Two days after, transfectants were selected by FACS, an antibiotic independent process, using GFP as the reporter gene (controled by IRES). Two more sortings rounds were necessary to stablish a 100% transfectant stable population. Cell culture supernatant was collected, and protein of interest was analysed by Western blot and ELISA.","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125184878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}