Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos最新文献

{"title":"Study to increase the disinfection holding time for classified areas at Bio-Manguinhos Formulation Division between two productive process","authors":"D. Oliveira, M. Antunes, R. Silva, Felipe Silva, G. Barros, D. Santos, Carla Freixo","doi":"10.35259/isi.sact.2019_32860","DOIUrl":"https://doi.org/10.35259/isi.sact.2019_32860","url":null,"abstract":"","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132967090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Developing an intranasal vaccine against canine visceral leishmaniasis: a study of efficacy in mice","authors":"I. Bezerra, B. Rossi-Bergmann","doi":"10.35259/isi.sact.2019_32544","DOIUrl":"https://doi.org/10.35259/isi.sact.2019_32544","url":null,"abstract":"","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"117 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115588347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative study of Vero Cell growth and virus infection in two different Serum-Free Medium (SFM)","authors":"K. Lúcio, Viviane Rodrigues, Guilherme Silva, Y. Mendes, S. M. Lima","doi":"10.35259/ISI.SACT.2019_32834","DOIUrl":"https://doi.org/10.35259/ISI.SACT.2019_32834","url":null,"abstract":"elongated shape in VP-SFM, in comparison with a slightly branched characteristic in Pro Vero-1 in the first 48h. Once confluent, they recovered their characteristic square shape, similar to cultivation in 199 medium. However, after 72 h of culture, VP-SFM medium presented a better performance and adaptation than ProVERO-1, probably due to the absence of epidermal growth factors in the latter. Cultures in VP-SFM medium showed similar performance to Vero cell cultures in 199 medium, although with different morphological characteristics in the first 24 h. However, neither VP-SFM nor ProVERO-1 reached the yields achieved by CHIKV production in 199 culture media at 48 h post infection. Conclusion : The results showed that Vero cell lineage was able to grow in both culture media tested, although the VP-SFM medium showed better performance, regarding adaptation and viral production when compared with ProVERO-1. For the production of CHIKV in Vero cells, however, data suggest the need for optimization of the conditions, since the parameters used in this preliminary study were standardized for production in medium supplemented with FBS.","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122499331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The management of Brazilian genetic resources in a public translational research center in accordance with the new legal framework","authors":"F. Pimenta, P. B. Zorzal","doi":"10.35259/isi.sact.2019_32764","DOIUrl":"https://doi.org/10.35259/isi.sact.2019_32764","url":null,"abstract":"Introduction: The biodiversity has been used as a model for generation of solutions to the society. And the knowledge about natural products and processes has been used as a support for many product developments or in relevant scientific researches. Since the publication of Provisional Act 2,186-16 / 2001 (PA), in force since 2000, the protection of Brazilian genetic resources (GR) and associated traditional knowledge (TK) is the subject of a rather controversial debate. This scenario was restrained by the new legal framework, in force since 2015, and better understood by the decree publication in 2016. Due to the new rules and requirements, the institutions must to be regularized in face of the legal uses of Brazilian GR and TK.","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"08 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127444382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experimental strategy to identify and validate membrane proteins as a diagnostic and therapeutic target for breast cancer","authors":"J. Mendonça, M. Waghabi, T. Tilli","doi":"10.35259/ISI.SACT.2019_32713","DOIUrl":"https://doi.org/10.35259/ISI.SACT.2019_32713","url":null,"abstract":"","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125797423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of 3D culture of mammary tumor cells for in vitro therapeutic response studies targeting personalized anticancer therapy","authors":"L. L. Coelho, Matheus Menezes Vianna, C. F. Lima, M. A. Moreira, A. Bonomo, C. Coutinho, F. Vargas, L. Garzoni","doi":"10.35259/isi.sact.2019_32813","DOIUrl":"https://doi.org/10.35259/isi.sact.2019_32813","url":null,"abstract":"Introduction: Breast cancer is the second most common type of cancer in the world. Personalized therapy is an option in the fight for the cure of cancer, since tumor variability is a great challenge in the elaboration of therapeutic protocols. Only 5% of the compounds tested in vitro in 2D systems present in vivo antitumor activity. Otherwise, the three-dimensional (3D) cell culture systems, which better mimic the architecture and tumor behavior observed in vivo, respond to in vitro treatment in a similar way to tumors in patients when treated with the same chemotherapeutic agents, showing great potential for evaluation of specific tumor therapy.","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"208 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114757628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of a scFv library using directed evolution for rituximab-based therapies: using phage display towards antibody affinity maturation","authors":"M. Bezerra, A. Maranhão, I. Studart, Larissa Queiroz Pontes, M. Fonseca, G. Furtado","doi":"10.35259/ISI.SACT.2019_32708","DOIUrl":"https://doi.org/10.35259/ISI.SACT.2019_32708","url":null,"abstract":"","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121801764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-CD19 CAR-T engineering through Molecular Dynamics Simulation","authors":"N. F. Frota, M. Maia, A. Rebouças, M. Lourenzoni","doi":"10.35259/isi.sact.2019_32705","DOIUrl":"https://doi.org/10.35259/isi.sact.2019_32705","url":null,"abstract":"","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"99 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124091516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preliminary studies of reactions using colominic and sialic acids as prototypes to bivalent vaccine against S. agalactiae and A. baumannii","authors":"Marcelio Oliveira, M. B. Corrêa, M. Leal, B. Silva, E. Jessouroun","doi":"10.35259/isi.sact.2019_32596","DOIUrl":"https://doi.org/10.35259/isi.sact.2019_32596","url":null,"abstract":"Introduction: Bioconjugation reactions play an important role in developing molecular arrangement of some different chemical species in new assembled structures. Such structures are of great importance in the vaccinology field against important pathogens. The pathogen S. agalactiae appears as a threat of invasive infection in neonates, characterizing a common problem to several countries. Similarly, the bacterium A. baumannii has been a problem in Brazilian hospitals, mainly due to the increasing development of resistance to different antimicrobial drugs. Therefore, a use of conjugate bivalent vaccines rises as an answer to prevention of nosocomial infections caused by this kind of pathogens. Hence, the development of new chemical approaches for synthesis of bivalent vaccines is imperative. A primary step for development of new methodologies about oxidation using prototypes that mimic functional groups present in S. agalactiae matrix was realized.","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125468711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Host-genetic combination based on IFNL3/IFNL4 polymorphism with other prognostic variables increases sustained response in antiviral therapy with pegylated interferon alpha","authors":"A. A. D. Silva, L. E. Alvarado-Arnez, Tamiris Azamor, L. R. Batista-Silva, Thyago Leal Calvo, J. Silva, A. C. A. Andrade, J. Xavier, D. Matos, M. Moraes","doi":"10.35259/32844","DOIUrl":"https://doi.org/10.35259/32844","url":null,"abstract":"Introduction: Chronic hepatitis C (CHC) is the main cause of liver disease and hepatocellular carcinoma progress worldwide. Although direct-acting antivirals (DAAs) are the treatment of choice, there are still special cases in which peginterferon alfa (Peg-IFNα) therapy should be used, such as children and patients infected with hepatitis Virus C (HCV) genotype 3 (GT3). Over the past few years, many studies have identified predictive factors for sustained virologic response (SVR), while host or viral factors are related to a successful Peg-IFNα and ribavirin (RBV) therapy. Single nucleotide polymorphisms (SNPs) in the interferon lambda 3 e 4 region (IFNL3/4) are wellestablished as prognostic markers after Pegylated-Interferon-alpha/ribavirin (Peg-IFN-α/RBV) treatment for CHC. The SNPs rs12979860, rs8109886 and rs8099917 are representative of the IFNL3/4 locus, associated with SVR, in the Brazilian population. So, the combination of predictive factors to obtain SVR could aid personalized and appropriate treatment for the population. This work was submitted to Research Ethics Committee of CAAE: 46065015.6.0000.5248. Objective: Evaluate the contribution of host genetics, and other prognostic variables in CHC patients with treated with Peg-IFNα, in SVR. Methodology: Three IFNL3-IFNL4 SNPs (rs12979860, rs8109886 and rs8099917) were genotyped by allelic discrimination in 632 chronic hepatitis C patients infected GT1, GT2 or GT3 treated with Peg-IFN-α/RBV, samples from a phase II/III randomized double-blind clinical trial. Serum samples of a subgroup of patients and healthy volunteers were used to measure CCL3, CCL4 and CXCL10, using liquid bead microarray assay. Results: Individually, either rs12979860-CC, rs8109886-CC or rs8099917-TT genotypes are predictive markers of SVR. The combination of these three genotypes (CC-CC-TT) increased to 73% at GT1 and 83% at GT3 of the rate of SVR. In contrast, patients infected with GT1 and homozygous for risk genotypes (TT-AA-GG) only 18% achieved SVR. Focusing on personalised therapy, patients with CC-CC-TT haplotype, HCV GT1-infected with viral load <5,9UI/mL Log10 showed an increased 82% SVR, while HCV GT2/3-infected patients with viral load <5,9UI/mL Log10 or F0 and F1 showed at least 93% and 96% SVR, respectively. The levels of the chemokines CCL3, CCL4 decreased after starting treatment, whereas, an increase of CXCL10 levels was observed in the first week, with a decreased over the course of the treatment. Higher levels of these chemokines presented an association with non-responsiveness after treatment. Conclusion: The analysis of rs12979860, rs8109886 and rs8099917 genotypic combination might be an approach when Peg-IFN-α/RBV therapy is necessary since cost-benefit for individuals carrying CC-CC-TT genotypic combination is very high, especially in countries in which this is the standard treatment, contributing to public health efforts to eradicate disease.","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127857246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}