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Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos最新文献

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Characterization of enzymatic activity and evaluation of cytotoxic effect of a Bacillus subtilis L-asparaginase type II expressed in Escherichia coli 大肠杆菌中表达的枯草芽孢杆菌l -天冬酰胺酶II型酶活性表征及细胞毒作用评价
Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos Pub Date : 1900-01-01 DOI: 10.35259/isi.sact.2019_32704
Ludmilla Freire Caetano, A. Torres, D. Gomes, M. Fonseca, C. Pessoa, G. Furtado
{"title":"Characterization of enzymatic activity and evaluation of cytotoxic effect of a Bacillus subtilis L-asparaginase type II expressed in Escherichia coli","authors":"Ludmilla Freire Caetano, A. Torres, D. Gomes, M. Fonseca, C. Pessoa, G. Furtado","doi":"10.35259/isi.sact.2019_32704","DOIUrl":"https://doi.org/10.35259/isi.sact.2019_32704","url":null,"abstract":"","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133377939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Metastasis of HPV-negative oral cavity tumors: an in silico study hpv阴性口腔肿瘤的转移:一项计算机研究
Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos Pub Date : 1900-01-01 DOI: 10.35259/isi.sact.2019_32824
M. Pretti, M. Boroni, N. Scherer, Frederico Gleber, I. Silva, D. Nunes, Jaqueline Ramalho, E. D. Neto, M. Bonamino
{"title":"Metastasis of HPV-negative oral cavity tumors: an in silico study","authors":"M. Pretti, M. Boroni, N. Scherer, Frederico Gleber, I. Silva, D. Nunes, Jaqueline Ramalho, E. D. Neto, M. Bonamino","doi":"10.35259/isi.sact.2019_32824","DOIUrl":"https://doi.org/10.35259/isi.sact.2019_32824","url":null,"abstract":"Methodology: 20 samples from a collaborator’s cohort (AC Camargo ACC) and 22 samples from The Cancer Genome Atlas (TCGA) were analyzed. The raw data from ACC was filtered and quality checked using FastQC and Trimmomatic, respectively. The ACC reads were then aligned against the human genome GRCh38 using star method and genes were counted with RSEM package. TCGA cohort already possess pre-processed data. Differentially expressed genes were evaluated by DESeq2, the enriched pathways by Webgestalt and tumor microenvironment by xCell. Exclusively for the TCGA available data, the HLA-I alleles were identified by Optitype and subsequent neoantigen prediction was accomplished by netMHCpan. T and B-cell receptors (TCR and BCR) repertoire were identified and analyzed by MiXCR.","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133647542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Polymeric nanoparticles as immunoadjuvant for oral immunization 聚合纳米颗粒作为口服免疫的免疫佐剂
Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos Pub Date : 1900-01-01 DOI: 10.35259/isi.sact.2019_32529
Juliana De Souza Rebouças, F. R. Formiga, J. Irache, C. Gamazo
{"title":"Polymeric nanoparticles as immunoadjuvant for oral immunization","authors":"Juliana De Souza Rebouças, F. R. Formiga, J. Irache, C. Gamazo","doi":"10.35259/isi.sact.2019_32529","DOIUrl":"https://doi.org/10.35259/isi.sact.2019_32529","url":null,"abstract":"","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"119 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133813823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification of viral populations and genome stability of current Brazilian yellow fever vaccine strains using whole genome data 利用全基因组数据鉴定当前巴西黄热病疫苗株的病毒种群和基因组稳定性
Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos Pub Date : 1900-01-01 DOI: 10.35259/isi.sact.2019_32592
Amanda Araújo Serrão Andrade, A. E. Soares, L. G. Almeida, C. Pestana, C. Aquino, Ana Teresa Ribeiro de Vaconcelos, M. Medeiros
{"title":"Identification of viral populations and genome stability of current Brazilian yellow fever vaccine strains using whole genome data","authors":"Amanda Araújo Serrão Andrade, A. E. Soares, L. G. Almeida, C. Pestana, C. Aquino, Ana Teresa Ribeiro de Vaconcelos, M. Medeiros","doi":"10.35259/isi.sact.2019_32592","DOIUrl":"https://doi.org/10.35259/isi.sact.2019_32592","url":null,"abstract":"Introduction: Yellow fever is a viral hemorrhagic fever caused by the mosquito-borne yellow fever flavivirus (YFV). There are currently two YFV attenuated vaccine strains that are used worldwide to prevent yellow fever, which were independently derived, 17DD (Bio-Manguinhos/ Fiocruz) and 17D-204 (World Health Organization). Sequencing and assembling the virus genomes of vaccine stocks can provide invaluable insights into viability and stability of vaccine strains, as maintaining stable vaccine strains is essential for vaccine mass production.","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114081452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of CAR T therapy for ALL-B using the point of care approach CAR - T治疗急性淋巴细胞白血病的进展
Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos Pub Date : 1900-01-01 DOI: 10.35259/ISI.SACT.2019_32857
L. Abdo, L. Barros, M. Duarte, L. V. C. Marques, L. Peixoto, M. Bonamino
{"title":"Development of CAR T therapy for ALL-B using the point of care approach","authors":"L. Abdo, L. Barros, M. Duarte, L. V. C. Marques, L. Peixoto, M. Bonamino","doi":"10.35259/ISI.SACT.2019_32857","DOIUrl":"https://doi.org/10.35259/ISI.SACT.2019_32857","url":null,"abstract":"Introduction: The global cancer data released by the GLOBOCAN database showed 18.3 million new cases in 2018. About 440,000 of these cases correspond to leukemia, a cancer that urges for treatment modalities. Recently, CAR T-cell immunotherapy was approved for the treatment of acute B cell leukemias (B-ALL) and some lymphomas with promising results. However, the major drawbacks of CAR-T treatments are the high costs, being prohibitive for many of the patients. We developed an alternative low-cost approach to gene modify T cells to express CAR using the Sleeping Beauty (SB) system and electroporation. In addition, we show that it is not necessary to activate or expand T cells ex vivo when using this system, an aspect that renders this approach to a point-of-care (POC) strategy.","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123720759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Standardization of Plaque Reduction Neutralization Test on 96-well Plates (micro-PRNT) for Zika Virus 兹卡病毒96孔板(micro-PRNT)空斑减少中和试验的标准化
Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos Pub Date : 1900-01-01 DOI: 10.35259/ISI.SACT.2019_32803
M. Simões, Vanessa de Oliveira Santos, J. Linhares, B. Setatino, S. Silva, E. Miranda, S. M. Lima
{"title":"Standardization of Plaque Reduction Neutralization Test on 96-well Plates (micro-PRNT) for Zika Virus","authors":"M. Simões, Vanessa de Oliveira Santos, J. Linhares, B. Setatino, S. Silva, E. Miranda, S. M. Lima","doi":"10.35259/ISI.SACT.2019_32803","DOIUrl":"https://doi.org/10.35259/ISI.SACT.2019_32803","url":null,"abstract":"Introduction: Plaque Reduction Neutralization Test (PRNT) is considered the “gold standard” by the World Health Organization for the confirmatory diagnosis of Flavivirus infection from the determination of neutralizing antibodies. Zika virus (ZIKV), a Flavivirus widely circulating in Brazil has caused considerable epidemic impact. Although 80% of cases are asymptomatic, virus infection in symptomatic cases causes headache, low fever, mild joint pain, red spots on the skin, itching and redness in the eyes. Other less common symptoms are swelling in the body, sore throat, coughing and vomiting. Infection by ZIKV has great importance especially for pregnant women, since the virus is a potential cause for the birth of children with a congenital malformation, in which the brain does not develop properly, called microcephaly. The Virological Technology Laboratory (LATEV, Bio-Manguinhos/Fiocruz) performs PRNT assays for different Flavivirus species, not only as confirmatory differential diagnosis, but mainly in the evaluation of the immunogenicity of commercial vaccines in development. Bio-Manguinhos currently participates in the development of three different vaccine proposals for the Zika virus. Therefore, considering potential increase in the demand for neutralizing antibody titers for diagnostic and vaccine evaluation, the standardization of a neutralization test for the Zika virus with high sample processing capacity meets the needs of LATEV and, consequently, of Institution and public health.","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122241841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of an identification system of Neisseria meningitidis, Streptococcus pneumoniae and Haemophilus influenzae in clinical samples, by qPCR-HRM 应用qPCR-HRM技术建立临床样品中脑膜炎奈瑟菌、肺炎链球菌和流感嗜血杆菌的鉴定系统
Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos Pub Date : 1900-01-01 DOI: 10.35259/isi.sact.2019_32770
I. Filippis, C. Andrade, A. C. Azevedo, A. Almeida
{"title":"Development of an identification system of Neisseria meningitidis, Streptococcus pneumoniae and Haemophilus influenzae in clinical samples, by qPCR-HRM","authors":"I. Filippis, C. Andrade, A. C. Azevedo, A. Almeida","doi":"10.35259/isi.sact.2019_32770","DOIUrl":"https://doi.org/10.35259/isi.sact.2019_32770","url":null,"abstract":"","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126913471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Production and characterization of Chikungunya virus for strategic antigen development to support public health demands 基孔肯雅病毒的生产和特性，用于战略性抗原的开发，以支持公共卫生需求
Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos Pub Date : 1900-01-01 DOI: 10.35259/ISI.SACT.2019_32540
Y. Mendes, Renata Tourinho Santos, Mariana Gomes, R. C. Pereira, D. F. Vieira, F. C. Jácome, G. Caldas, S. M. Lima, Marta Cristina O Souza, G. Trindade
{"title":"Production and characterization of Chikungunya virus for strategic antigen development to support public health demands","authors":"Y. Mendes, Renata Tourinho Santos, Mariana Gomes, R. C. Pereira, D. F. Vieira, F. C. Jácome, G. Caldas, S. M. Lima, Marta Cristina O Souza, G. Trindade","doi":"10.35259/ISI.SACT.2019_32540","DOIUrl":"https://doi.org/10.35259/ISI.SACT.2019_32540","url":null,"abstract":"Methodology: Vero cells infection by CHIKV was conducted with a Multiplicity of Infection (MOI) of 1.0. Monitoring virus entry, cells were fixed, embedded in polymers, ultrathin cut and analyzed by transmission electron microscopy (TEM). Quantification of CHIKV was performed by real-time PCR (qPCR), for the particles inside the cell and in the supernatant, and by plaque assay for supernatant. Investigating the best conditions for CHIKV production, kinetics were conducted in two MOIs (0.01 and 0.005) for 48 h. Production in serum-free media was also tested. Kinetics were monitored by cytopathic effect (CPE), plaque assay and qPCR.","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128710121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of the antitumor effect of Natural Killer cells expressing Chimeric Antigen Receptors (CAR) against murine melanoma cells 表达嵌合抗原受体(CAR)的自然杀伤细胞对小鼠黑色素瘤细胞抗肿瘤作用的评价
Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos Pub Date : 1900-01-01 DOI: 10.35259/isi.sact.2019_32697
M. Duarte, L. Abdo, L. Barros, M. Bonamino
{"title":"Evaluation of the antitumor effect of Natural Killer cells expressing Chimeric Antigen Receptors (CAR) against murine melanoma cells","authors":"M. Duarte, L. Abdo, L. Barros, M. Bonamino","doi":"10.35259/isi.sact.2019_32697","DOIUrl":"https://doi.org/10.35259/isi.sact.2019_32697","url":null,"abstract":"","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123981747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Technology foresight for the identification of new targets, opportunities and partnerships in plant-based platforms 技术预见，以确定植物基平台的新目标、机会和伙伴关系
Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos Pub Date : 1900-01-01 DOI: 10.35259/ISI.SACT.2019_32778
D. Freire, L. Faria, Tamiris A C Barros, Milena Carvalho, C. Marques, Marilza B. Corrêa, B. Fialho
{"title":"Technology foresight for the identification of new targets, opportunities and partnerships in plant-based platforms","authors":"D. Freire, L. Faria, Tamiris A C Barros, Milena Carvalho, C. Marques, Marilza B. Corrêa, B. Fialho","doi":"10.35259/ISI.SACT.2019_32778","DOIUrl":"https://doi.org/10.35259/ISI.SACT.2019_32778","url":null,"abstract":"Introduction: As an example of many of technology foresight (TF) applications, it can help an organization to identify areas to concentrate research and development investments in short, medium and long terms. Since 2012, Bio-Manguinhos (BM) has been investing in plant-based technologies. This is an emerging field, with few registered products and only one in the market. Therefore, establishing a systematic TF will contribute to BM’ decisionmaking for target selection and project prioritization to be further advanced at the Plant Based Technology Center.","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123791533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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