Establishment of 3D culture of mammary tumor cells for in vitro therapeutic response studies targeting personalized anticancer therapy

Abstract

Introduction: Breast cancer is the second most common type of cancer in the world. Personalized therapy is an option in the fight for the cure of cancer, since tumor variability is a great challenge in the elaboration of therapeutic protocols. Only 5% of the compounds tested in vitro in 2D systems present in vivo antitumor activity. Otherwise, the three-dimensional (3D) cell culture systems, which better mimic the architecture and tumor behavior observed in vivo, respond to in vitro treatment in a similar way to tumors in patients when treated with the same chemotherapeutic agents, showing great potential for evaluation of specific tumor therapy.