Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos最新文献_第6页

Phylogenetic approaches to evaluate the application of qPCR designed for measles vaccine strains for diagnosis 评价麻疹疫苗株qPCR诊断应用的系统发育方法

Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos Pub Date : 1900-01-01 DOI: 10.35259/ISI.SACT.2019_32541

G. Trindade, Xênia R Lemos, Cristiane Pestana, L. Morais, Renata Santos, Juliana Silva, S. M. Lima

引用次数: 0

Long template RT-PCR optimization to amplify the first complete genome of Hepatitis C Virus subtype 2b from Latin America 拉丁美洲丙型肝炎病毒2b亚型首个完整基因组的长模板RT-PCR优化

Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos Pub Date : 1900-01-01 DOI: 10.35259/isi.sact.2019_32707

N. Spitz, J. J. Barros, Catarina Santana, C. Mello, N. M. Araujo

引用次数: 0

Synergistic effect between silver nanoparticles and amphotericin B on pathogenic fungi 纳米银粒子与两性霉素B对病原菌的协同作用

Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos Pub Date : 1900-01-01 DOI: 10.35259/isi.sact.2019_32738

A. Figueiredo, Gabriel M Misirli, M. Rodrigues, F. Conte

引用次数: 2

Biological relevance of A2a receptor in Schwann cells infected by Mycobacterium leprae: a possible pathway involved in leprosy? 麻风分枝杆菌感染雪旺细胞中A2a受体的生物学相关性:参与麻风的可能途径?

Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos Pub Date : 1900-01-01 DOI: 10.35259/ISI.SACT.2019_32833

Plínio Marcos Freire dos Santos, B. S. Mietto, L. Gutierres, Antônio Marcos Rodrigues Pereira, C. C. Acosta, M. C. Pessolani, Marcia de Berrêdo Pinho Moreira

{"title":"Biological relevance of A2a receptor in Schwann cells infected by Mycobacterium leprae: a possible pathway involved in leprosy?","authors":"Plínio Marcos Freire dos Santos, B. S. Mietto, L. Gutierres, Antônio Marcos Rodrigues Pereira, C. C. Acosta, M. C. Pessolani, Marcia de Berrêdo Pinho Moreira","doi":"10.35259/ISI.SACT.2019_32833","DOIUrl":"https://doi.org/10.35259/ISI.SACT.2019_32833","url":null,"abstract":"Introduction: Leprosy is a chronic infectious disease caused by Mycobacterium leprae which evokes a strong inflammatory response and leads to nerve damage. Two important aspects that favor the establishment of infection and survival of M. leprae are: lipid droplet accumulation and the capacity to induce rapid demyelination after extracellular binding to myelinating Schwann cells (SC). Recently, purinergic receptors have been shown to participate in myelination processes, lipid metabolism and immune response. In addition, the purinergic signaling system plays an important role by modulating inflammatory and immune responses, via extracellular adenine nucleotides and their derived nucleoside adenosine (ADO). The enzymatic activities of CD39, CD73 and Adenosine deaminase play strategic roles in regulating purinergic signals through the conversion of ADP/ATP to AMP, AMP to adenosine and adenosine to inosine respectively.","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"141 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132695425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High Proteasome Activity in Plasma of Patients with Hematologic Malignancy (Case of 145 Moroccan patients) 血液恶性肿瘤患者血浆高蛋白酶体活性分析(附145例摩洛哥患者报告)

Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos Pub Date : 1900-01-01 DOI: 10.35259/isi.sact.2019_32732

F. Hassan

引用次数: 0

Improvement of seroneutralization assays throughput and accuracy by the automation of image acquisition and analysis 通过图像采集和分析自动化提高血清中和分析的吞吐量和准确性

Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos Pub Date : 1900-01-01 DOI: 10.35259/isi.sact.2019_32542

W. Schwarcz, C. Denani, R. Vieira, F. Luca, J. Linhares, M. Borges, S. M. Lima

引用次数: 0

Rapid colorimetric detection of Zika Virus from mosquito samples by Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) 逆转录环介导等温扩增(RT-LAMP)快速比色法检测蚊媒寨卡病毒

Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos Pub Date : 1900-01-01 DOI: 10.35259/isi.sact.2019_32796

Severino Jefferson Ribeiro da Silva, M. H. S. Paiva, D. R. D. Guedes, Larissa Krokovsky, F. Melo, C. F. J. Ayres, L. Pena

引用次数: 0

Organization handbook: document directs regarding papers and responsibilities in a public organizational structure 组织手册:公共组织结构中有关文件和职责的文件指导

Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos Pub Date : 1900-01-01 DOI: 10.35259/ISI.SACT.2019_32793

C. Silva, Aloysio Moreira Junior

引用次数: 0

Human pegivirus challenge: detection and molecular characterization in HCV/HIV coinfected individuals 人类培吉病毒攻击:HCV/HIV合并感染个体的检测和分子特征

Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos Pub Date : 1900-01-01 DOI: 10.35259/ISI.SACT.2019_32865

J. Pereira, J. J. Barros, C. Brandão, V. Paula, C. C. Soares

{"title":"Human pegivirus challenge: detection and molecular characterization in HCV/HIV coinfected individuals","authors":"J. Pereira, J. J. Barros, C. Brandão, V. Paula, C. C. Soares","doi":"10.35259/ISI.SACT.2019_32865","DOIUrl":"https://doi.org/10.35259/ISI.SACT.2019_32865","url":null,"abstract":"is rare. Objective: The aim of this study was to determine the prevalence and genotypic distribution of HPgV in patients attended at a hospital in Rio de Janeiro. Methodology : A RT-PCR assay for specific amplification of 5´UTR region of HPgV genome was performed in 174 serum samples collected from patients under health treatment at a hospital in Rio de Janeiro. The samples were classified into three groups: 56 samples form HCV/HIV coinfected individuals; 58 from HCV mono-infected and 60 from HIV monoinfected individuals. All positive samples were submitted to direct sequencing for genotyping and molecular characterization. Results: The overall prevalence of HPgV-1 was 17.2% (30/174). Among HCV/HIV coinfected patients, HPgV prevalence was 14.3% (8/56), and all of them were successfully sequenced. Phylogenetic analysis revealed the presence of genotypes 2a (12.5%), 2b (62.5%) and 3 (25%). HPgV was also found in coinfection with HCV (8.6%; 5/58) and HIV (28.3%; 17/60). Conclusion : Our findings demonstrate the high frequency of HPgV among HCV/HIV coinfected, HCV and HIV monoinfected individuals attending a public hospital in Rio de Janeiro. Circulating HPgV genotypes described here have already been reported in past Brazilian studies, but this is the first data about HCV/HIV patients in Rio de Janeiro city. This study intends to contribute with insights about epidemiological characteristics and impact (if there is any) of HPgV in the natural course of HCV and/ or HIV infection.","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121252831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of Quantitative Real-Time PCR (TaqMan Triplex System) for the Diagnosis and Evaluation of Therapeutic Efficacy in Chagas Disease 实时荧光定量PCR (TaqMan三重系统)在恰加斯病诊断和疗效评价中的应用

Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos Pub Date : 1900-01-01 DOI: 10.35259/isi.sact.2019_32808

C. Britto, Carolina V. Messias, O. Moreira, A. Ferreira, R. Sýkora, M. Krieger, A. Costa, A. Gomes, Jéssica Constermani

引用次数: 0