Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos最新文献_第5页

Influence of differents cryoprotecttive agents on the maintenance of Vero cells adapted to serum-free culture media 不同冷冻保护剂对Vero细胞适应无血清培养基维持的影响

Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos Pub Date : 1900-01-01 DOI: 10.35259/ISI.SACT.2019_32828

K. Lúcio, Viviane Rodrigues, Guilherme Silva, Mariana Gomes, Marta Cristina O Souza, S. M. Lima

{"title":"Influence of differents cryoprotecttive agents on the maintenance of Vero cells adapted to serum-free culture media","authors":"K. Lúcio, Viviane Rodrigues, Guilherme Silva, Mariana Gomes, Marta Cristina O Souza, S. M. Lima","doi":"10.35259/ISI.SACT.2019_32828","DOIUrl":"https://doi.org/10.35259/ISI.SACT.2019_32828","url":null,"abstract":"+ 5% Gl + 1% F68; vii) VP-SFM + 5% Gl + 10% DMSO; viii) VP-SFM + 5% Gl + 10% DMSO + 1% F68. After thawing, recovery and cell growth in VP-SFM medium ware evaluated for 4 passages. Results: We evaluated isolated and synergic effect of three CPAs after the thawing Vero cells. The best result obtained were VP-SFM medium in the presence of 10% DMSO and 10% DMSO + 1% F68. Post-thaw viability and morphology were preserved in both situations. At 10% DMSO + 5% F68 the protective effect was lost due to the high concentration of F68. In all conditions in which glycerol was added no growth promotion was observed, demonstrating that glycerol is not a good option for Vero cell cryopreservation. Conclusion : The criopreservation is a valuable tool for cells preservation and the success of this procedure depends on the proper use of CPAs. Although there is no ideal CPA, able to completely protect cells at low temperatures and be free of toxicity, it is clear that only the combination of DMSO and F68 are satisfactory for cryopreserving Vero cells in the absence of FBS and that glycerol is not an option in these tested conditions.","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129288456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effectiveness of 2018 trivalent influenza vaccine on healthcare professionals 2018年三价流感疫苗对医护人员的有效性

Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos Pub Date : 1900-01-01 DOI: 10.35259/isi.sact.2019_32545

A. Capão, B. Caetano, P. Resende, M. Miranda, David Brown, M. Siqueira, Cristiana C Garcia

引用次数: 0

Two innovative multi-epitope recombinant proteins for the diagnosis of chronic T. cruzi infections 两种新型多表位重组蛋白用于慢性克氏锥虫感染的诊断

Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos Pub Date : 1900-01-01 DOI: 10.35259/isi.sact.2019_32765

A. Durans, Flavia Reis, F. R. Carneiro, P. Pêgo, Evandro Dias, S. Simone, A. Junqueira, D. Provance

引用次数: 0

Usefulness of IgY technology for hepatitis B virus diagnosis IgY技术在乙型肝炎病毒诊断中的应用

Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos Pub Date : 1900-01-01 DOI: 10.35259/ISI.SACT.2019_32773

R. Deodato, D. Santos, H. M. Cruz, C. Lima, Carolina Soares van der Meer, L. Villar

{"title":"Usefulness of IgY technology for hepatitis B virus diagnosis","authors":"R. Deodato, D. Santos, H. M. Cruz, C. Lima, Carolina Soares van der Meer, L. Villar","doi":"10.35259/ISI.SACT.2019_32773","DOIUrl":"https://doi.org/10.35259/ISI.SACT.2019_32773","url":null,"abstract":"an (ELISA) for of affinity chromatography. IgY anti-HBs using polyacrylamide gel electrophoresis (SDS-PAGE), dot blot and enzyme-linked immunosorbent assay (ELISA). Total protein concentrations were measured using spectrophotometer. Samples presenting higher total protein concentration were evaluated as capture protein for in house ELISA to detect HBsAg. Results: The characterization of IgY revealed bands of stained peptides with molecular weight between 75 and 50kDa and 37 and 25kDa using SDS Page. In the dot blot test was observed that there was antigen-antibody reaction throughout the sample period. Mean ± standard deviation (SD) of concentration of total protein obtained after purification by affinity chromatography was 0.386 mg / mL ± 0.440 mg/mL. After evaluation of different dilutions of IgY as capture protein for ELISA, it was possible to distinguish positive from negative controls that presented mean ±SD of optical density (OD) values of 1.99 ± 0.18 and 0.45 ± 0.06, respectively. Conclusion : The results indicated that HBV is able to generate a high production of specific immunoglobulins in chickens and these proteins could be used as capture protein for ELISA. Further studies will be conducting to develop in house ELISA for hepatitis B diagnosis.","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114286737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Brazilian Genetic Heritage Act and its impact on Bio-Manguinhos’ scientific publications 巴西遗传遗产法案及其对Bio-Manguinhos科学出版物的影响

Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos Pub Date : 1900-01-01 DOI: 10.35259/ISI.SACT.2019_32797

L. Faria, C.R.X. Costa

{"title":"Brazilian Genetic Heritage Act and its impact on Bio-Manguinhos’ scientific publications","authors":"L. Faria, C.R.X. Costa","doi":"10.35259/ISI.SACT.2019_32797","DOIUrl":"https://doi.org/10.35259/ISI.SACT.2019_32797","url":null,"abstract":"Introduction: The Brazilian Genetic Heritage Act (13.123) was enforced on November 2015. It seeks to protect and to encourage the sustainable use of Brazilian biodiversity. The Act defines Genetic Heritage (GH) as information on the genetic origin of vegetal, animal and microbial species, or species of other nature, and on the substances derived from the metabolism of those living beings. Therefore, the Act encompasses every scientific activity utilizing viruses/ bacteria/fungi which have been collected from Brazilian individuals or endogenous animals; Brazilian genetic sequences from GenBank®; in silico research of endogenous viruses/bacteria/ fungi; microorganisms engineered with endogenous genetic sequences; antibodies produced by endogenous animals, domesticated species and spontaneous populations found in Brazilian territory. Among the legal demands there is a mandatory registration in the National System for the Management of Genetic Heritage and Associated Traditional Knowledge (SisGen) prior to the disclosure of either final or parcial results of researches using endogenous GH.","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"61 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114722744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adalimumab protection trough patents of invention: a comparison of the strategies adopted by the American, Brazilian and European Patent Offices 通过发明专利保护阿达木单抗:美国、巴西和欧洲专利局采用的策略比较

Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos Pub Date : 1900-01-01 DOI: 10.35259/ISI.SACT.2019_32842

M. Silva, I. Fierro

{"title":"Adalimumab protection trough patents of invention: a comparison of the strategies adopted by the American, Brazilian and European Patent Offices","authors":"M. Silva, I. Fierro","doi":"10.35259/ISI.SACT.2019_32842","DOIUrl":"https://doi.org/10.35259/ISI.SACT.2019_32842","url":null,"abstract":"Introduction: Biological drugs displayed an exponential development in recent years. Monoclonal antibodies (mAbs), in particular, have proved to be one of the main categories of these drugs, incorporating several innovations in their production. In order to protect these innovative products, patent protection has been widely used by companies, from startups to multinationals. However, although allowing the return of investments in the R&D of new drugs, patent protection creates an exploitation monopoly which leads to an increase of costs by national health systems. According to the Brazilian Ministry of Health, biological drugs represent 40% of the costs with drugs, but only 2% of the total drugs volume. These high costs may be due to the exceptions admitted by the sole paragraph of article 40 of the Brazilian Patent Statute, which allows the extension of the patent term if the examination takes more than 10 years, and to the Brazilian Patent Office delay in finishing the examination, the socalled backlog. Therefore, the identification of common points in patent examination among several patent offices could reduce the examination time and, ultimately, the backlog.","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"70 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127570105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ebola vaccine induced arthritis: gene predictors from a Random Forest Model 埃博拉疫苗诱导关节炎:随机森林模型的基因预测因子

Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos Pub Date : 1900-01-01 DOI: 10.35259/isi.sact.2019_32647

Patrícia Dias, H. Nakaya

引用次数: 0

Relevancy and applicability of Marketing activities and its strategic role by providing subsidies and support to key areas in Bio-Manguinhos 营销活动的相关性和适用性及其通过向生物-曼金诺斯的关键领域提供补贴和支持的战略作用

Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos Pub Date : 1900-01-01 DOI: 10.35259/isi.sact.2019_32769

L. K. Boukai, Julia V. A. Figueiredo, Denise Maria Lobo Crivelli

引用次数: 0

Health and cocriation a bibliometric analysis of literature 健康与合作:文献计量学分析

Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos Pub Date : 1900-01-01 DOI: 10.35259/isi.sact.2019_32795

C. Silva

引用次数: 0

Genetic basis for yellow fever vaccine-associated viscerotropic disease (YEL-AVD): a preliminary report 黄热病疫苗相关嗜内脏病(yelavd)的遗传基础:初步报告

Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos Pub Date : 1900-01-01 DOI: 10.35259/ISI.SACT.2019_32518

E. C. Mesquita, Deborah Araújo da Conceição, P. M. N. Oliveira, Tamiris A C Barros, Qian Zhang, Nicholas Hernandez, E. Jouanguy, J. Casanova, M. L. S. Maia, R. M. Martins

引用次数: 1