Influence of differents cryoprotecttive agents on the maintenance of Vero cells adapted to serum-free culture media

+ 5% Gl + 1% F68; vii) VP-SFM + 5% Gl + 10% DMSO; viii) VP-SFM + 5% Gl + 10% DMSO + 1% F68. After thawing, recovery and cell growth in VP-SFM medium ware evaluated for 4 passages. Results: We evaluated isolated and synergic effect of three CPAs after the thawing Vero cells. The best result obtained were VP-SFM medium in the presence of 10% DMSO and 10% DMSO + 1% F68. Post-thaw viability and morphology were preserved in both situations. At 10% DMSO + 5% F68 the protective effect was lost due to the high concentration of F68. In all conditions in which glycerol was added no growth promotion was observed, demonstrating that glycerol is not a good option for Vero cell cryopreservation. Conclusion : The criopreservation is a valuable tool for cells preservation and the success of this procedure depends on the proper use of CPAs. Although there is no ideal CPA, able to completely protect cells at low temperatures and be free of toxicity, it is clear that only the combination of DMSO and F68 are satisfactory for cryopreserving Vero cells in the absence of FBS and that glycerol is not an option in these tested conditions.