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Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos最新文献

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Identification of aptamers for application in the differential diagnosis of ovarian tumor 适配体在卵巢肿瘤鉴别诊断中的应用
Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos Pub Date : 1900-01-01 DOI: 10.35259/ISI.SACT.2019_32811
R. Abreu, A. S. Moreira, Fábio Passet, C. Bessa, W. Degrave, M. Waghabi
{"title":"Identification of aptamers for application in the differential diagnosis of ovarian tumor","authors":"R. Abreu, A. S. Moreira, Fábio Passet, C. Bessa, W. Degrave, M. Waghabi","doi":"10.35259/ISI.SACT.2019_32811","DOIUrl":"https://doi.org/10.35259/ISI.SACT.2019_32811","url":null,"abstract":"Introduction: Cancer is an important cause of worldwide mortality, killing more than 8 million people every year. The ovarian cancer is one of seven cancer types than most affect womem, and is considered the most fatal among the gynecologic tumors. The ovarian tumor detection presents importants limitations and is highlighted as the most difficult gynecological tumor to be diagosed, hidering the efficiency of the treatment and consequently patients’ survival. Specific molecules produced by tumors could be used as biomarkers and are considered good targets for diagnosis. Thus, the use of aptamers represents an important tool to be applied for the improvement of the specificity in tumor diagnosis. Aptamers are small synthetic nucleic acid sequences, which bind with high specificity to a molecular target, presenting interesting pharmacokinetic stabillity, bioavailability and permanence in blood circulation, and moreover are able to bioconjugation with nanoparticles, imaging agents and therapeutics drugs. Bioinformatics approaches may contribute to the improvement of aptamer identification and studies focusing in target molecule-aptamer interaction. Thus, computational analyzes are being applied to aptamers study, including 3D structural modeling, in silico simulation for aptamers selection by molecular dynamics, modeling of virtual libraries and in silico aptamer optimization.","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122462470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of different adjuvant formulations for development of an inactivated Yellow Fever vaccine candidate 评价不同佐剂配方用于研制黄热病灭活候选疫苗
Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos Pub Date : 1900-01-01 DOI: 10.35259/isi.sact.2019_32547
Ana Cajaraville, M. Pierre, R. C. Pereira, P. C. Neves, E. Caride, S. M. Lima, L. Gaspar, M. Medeiros
{"title":"Evaluation of different adjuvant formulations for development of an inactivated Yellow Fever vaccine candidate","authors":"Ana Cajaraville, M. Pierre, R. C. Pereira, P. C. Neves, E. Caride, S. M. Lima, L. Gaspar, M. Medeiros","doi":"10.35259/isi.sact.2019_32547","DOIUrl":"https://doi.org/10.35259/isi.sact.2019_32547","url":null,"abstract":"Introduction: Yellow Fever vaccine is one of the most successful vaccines ever developed. Nonetheless, restrictions of administration to immunosuppressed and rare adverse events are drawbacks that stimulate the development of safer non-live approaches. As safety increases, however, immunogenicity decreases due to the lack of viral replication. In this context, adjuvants are key elements to activate innate immunity and shape the desired adaptative responses and protection. Adjuvants of different mechanisms of action have been studied and we selected candidates of each class: antigen carriers (Al(OH)3 and Addavax) and immune potentiators (Flagellin). In this study we intend to identify promising adjuvants for development of an inactivated yellow fever virus (IYFV) vaccine candidate, in preclinical evaluation in mice model.","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"58 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121866996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification of breast cancer neoantigens using in silico methodologies 用计算机方法鉴定乳腺癌新抗原
Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos Pub Date : 1900-01-01 DOI: 10.35259/ISI.SACT.2019_32690
Nina Barreira, Rodrigo Silva, T. Tilli, P. C. Neves
{"title":"Identification of breast cancer neoantigens using in silico methodologies","authors":"Nina Barreira, Rodrigo Silva, T. Tilli, P. C. Neves","doi":"10.35259/ISI.SACT.2019_32690","DOIUrl":"https://doi.org/10.35259/ISI.SACT.2019_32690","url":null,"abstract":"Introduction: Cancer is a group of diseases that involve abnormal cell growth, with potential to invade and spread to other parts of the body forming secondary tumors, called metastasis. Neoplasms are the main cause of death in the world, mainly due to the metastasis. In Brazil, there are more than 600.000 new cases in 2018, and breast cancer is the most frequent among Brazilian women. The most promising therapies, at the time, to combat this disease are immunotherapies, which deal with the manipulation of the immune system to better respond against the tumor and eliminate it. Some metastatic cancers, such as breast cancer, are poorly immunogenic and therefore difficult to eradicate. However, specific mutations in tumor proteins can be considered as targets for cancer immunotherapy, since it could be recognized as neoantigens by host T cells, allowing the development of therapeutic vaccines.","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132743745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Plant-produced YFE-1T subunit vaccine against Yellow Fever induces humoral response in captive golden-headed lion tamarin (Leontopithecus chrysomelas) 植物产抗黄热病YFE-1T亚单位疫苗诱导圈养金头狮狨猴体液应答
Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos Pub Date : 1900-01-01 DOI: 10.35259/ISI.SACT.2019_32549
A. Fernandes, L. Gaspar, R. C. Pereira, Vanessa de Oliveira Santos, R. Marchevsky, S. B. Moreira, A. Pissinatti, E. Caride, R. C. Guimarães, M. Freire
{"title":"Plant-produced YFE-1T subunit vaccine against Yellow Fever induces humoral response in captive golden-headed lion tamarin (Leontopithecus chrysomelas)","authors":"A. Fernandes, L. Gaspar, R. C. Pereira, Vanessa de Oliveira Santos, R. Marchevsky, S. B. Moreira, A. Pissinatti, E. Caride, R. C. Guimarães, M. Freire","doi":"10.35259/ISI.SACT.2019_32549","DOIUrl":"https://doi.org/10.35259/ISI.SACT.2019_32549","url":null,"abstract":"Introduction: Yellow Fever (YF) is a mosquito-borne viral hemorrhagic fever with a case fatality rate up to 50%. It is endemic in the Brazilian Amazon region, and sporadic outbreaks take place outside the endemic area in Brazil. However, Brazil has recently experienced its largest recorded YF outbreak in decades, with 1,376 confirmed cases and 483 deaths in humans, and 864 confirmed epizooties in non-human primates (NHPs) from July 2017 to June 2018. Despite the excellent safety of YF 17DD vaccine, there are rare cases of serious adverse events, which are often fatal. According to described aspects, the risk of urban YF resurgence and the absence of a vaccine available for NHPs, there is a strong demand for the development of alternative YF vaccines that are safe and efficacious for disease control. Therefore, Bio-Manguinhos/Fiocruz, in collaboration with Fraunhofer USA Center for Molecular Biotechnology, has been developing a plant-derived recombinant subunit YF vaccine (YFE-1T) to address the global demand for increasing availability and safety of the YF vaccine. YFE-1T subunit composed by the ectodomain of the E protein was transiently expressed in Nicotiana benthamiana by Agrobacterium tumefaciens-mediated gene transfer, purified and adsorbed to aluminum hydroxide adjuvant.","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133514763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Prospection studies: a new methodology for strengthening research projects conducted by graduate students 前瞻性研究:加强研究生研究项目的新方法
Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos Pub Date : 1900-01-01 DOI: 10.35259/ISI.SACT.2019_32781
J. Santos-Gandelman, F. Pimenta
{"title":"Prospection studies: a new methodology for strengthening research projects conducted by graduate students","authors":"J. Santos-Gandelman, F. Pimenta","doi":"10.35259/ISI.SACT.2019_32781","DOIUrl":"https://doi.org/10.35259/ISI.SACT.2019_32781","url":null,"abstract":"","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131858540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Purification and evaluation of antifungal properties of lectin-Fc proteins against Aspergillus fumigatus 凝集素fc蛋白对烟曲霉抑菌性能的纯化及评价
Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos Pub Date : 1900-01-01 DOI: 10.35259/ISI.SACT.2019_32694
C. Noval, Marina da Silva Ferreira, D. S. Gonçalves, Yasmin Braga, R. Figueiredo, L. Nimrichter, A. Guimarães
{"title":"Purification and evaluation of antifungal properties of lectin-Fc proteins against Aspergillus fumigatus","authors":"C. Noval, Marina da Silva Ferreira, D. S. Gonçalves, Yasmin Braga, R. Figueiredo, L. Nimrichter, A. Guimarães","doi":"10.35259/ISI.SACT.2019_32694","DOIUrl":"https://doi.org/10.35259/ISI.SACT.2019_32694","url":null,"abstract":"","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134269226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The evaluation of suppliers for public pharmaceutical laboratories: regulatory and legal implications 公共制药实验室供应商的评价:法规和法律影响
Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos Pub Date : 1900-01-01 DOI: 10.35259/isi.sact.2019_32776
Driele Neves, C. Silva, F. Silva, B. Barreto
{"title":"The evaluation of suppliers for public pharmaceutical laboratories: regulatory and legal implications","authors":"Driele Neves, C. Silva, F. Silva, B. Barreto","doi":"10.35259/isi.sact.2019_32776","DOIUrl":"https://doi.org/10.35259/isi.sact.2019_32776","url":null,"abstract":"Introduction: Suppliers have a very important relevance in the supply chain. As a public institution, Bio-Manguinhos Institute is submitted to the implementation law’s which operate the public purchasing process for the acquisition of its raw materials, which includes but not limited to the Laws n° 8.666/93 and n° 10.520/02 . In addition, as a pharmaceutical manufacturer industry, it is also included in Resolution RDC n° 17, of April 16, 2010 of the National Agency of Sanitary Surveillance (ANVISA). The fact that to comply with the various applicable laws, it becomes a challenge for the supply area, since it must meet customer expectations and chain interfaces, whether internal or external.","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"130 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114595861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genetic algorithm for deimmunization of Escherichia coli L-asparaginase 大肠杆菌l -天冬酰胺酶脱免疫的遗传算法
Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos Pub Date : 1900-01-01 DOI: 10.35259/isi.sact.2019_32695
Raphael Trevizani
{"title":"Genetic algorithm for deimmunization of Escherichia coli L-asparaginase","authors":"Raphael Trevizani","doi":"10.35259/isi.sact.2019_32695","DOIUrl":"https://doi.org/10.35259/isi.sact.2019_32695","url":null,"abstract":"Introduction: L-asparaginase is a heterologous enzyme expressed in Escherichia coli and used in leukemya therapy that triggers an immune response due to the presence of linear T-cell epitopes within the protein sequence. This results in the sustained production of antibodies that causes hypersensitivity problems and in the neutralization of the therapeutic effect. To diminish the potential immunogenicity, the protein can be subject to a process of epitope deletion via genetic manipulation of key MHC-high-binding residues. As an exhaustive evaluation of the mutants is prohibitive for experimental scientist because the number of variants is too big, in silico tools can be used to diminish the search space.","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116761214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Vaccines Clinical Research Model at Basic Health Units in Rio de Janeiro 里约热内卢基础卫生单位的疫苗临床研究模式
Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos Pub Date : 1900-01-01 DOI: 10.35259/ISI.SACT.2019_32763
Maria Céu Santos, M. Maia, C. Motta, J. Figueira, D. Aguiar, D. Souza, Celia Menezes Cruz Marques
{"title":"Vaccines Clinical Research Model at Basic Health Units in Rio de Janeiro","authors":"Maria Céu Santos, M. Maia, C. Motta, J. Figueira, D. Aguiar, D. Souza, Celia Menezes Cruz Marques","doi":"10.35259/ISI.SACT.2019_32763","DOIUrl":"https://doi.org/10.35259/ISI.SACT.2019_32763","url":null,"abstract":"","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124456987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Screening of proteins related to the immunological checkpoint lymphocyte activation gene-3 (lag-3) through the BioID method BioID法筛选免疫检查点淋巴细胞活化基因-3 (lag-3)相关蛋白
Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos Pub Date : 1900-01-01 DOI: 10.35259/isi.sact.2019_32831
Priscila Ribeiro, L. Chicaybam, M. Pretti, M. Bonamino
{"title":"Screening of proteins related to the immunological checkpoint lymphocyte activation gene-3 (lag-3) through the BioID method","authors":"Priscila Ribeiro, L. Chicaybam, M. Pretti, M. Bonamino","doi":"10.35259/isi.sact.2019_32831","DOIUrl":"https://doi.org/10.35259/isi.sact.2019_32831","url":null,"abstract":"localization The CAR anti-CD20/Lag3 WT-BirA electroporated in the HEK293T cells presenting 80% of expression. CARs Ep del and Kmut showed 39% and 41% of CAR expression. it was possible to observe the cytoplasmatic localization of the CARs. Analysis of the biotinylation pattern by Western blotting was performed for CAR anti-CD20/Lag3WT-BirA, and the expected ladder pattern of biotinylation was observed. Conclusion : The constructed CARs were expressed in the target cell lines leading to the expected biotinilated patterns.","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121679522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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