Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos最新文献

{"title":"Development of a Meningococcal W Conjugate Vaccine","authors":"I. Souza, P. Guzzo, R. Bastos, F. Martins, M. Silva, E. Figueira, E. Jessouroun, M. Leal, E. Bergter, I. Silveira","doi":"10.35259/isi.sact.2019_32623","DOIUrl":"https://doi.org/10.35259/isi.sact.2019_32623","url":null,"abstract":"","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130832011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of active human herpesviruses 6 and 7 infection before and after renal transplantation","authors":"J. V. Raposo, Rafaela Barbosa da Silva Pinto, A. Lopes, Ana Paula Couto da Silva, T. Mendoza, Paulo Henrique Braz Silva, V. Paula","doi":"10.35259/ISI.SACT.2019_32830","DOIUrl":"https://doi.org/10.35259/ISI.SACT.2019_32830","url":null,"abstract":"","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130919229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A fully automated lab-on-a-chip platform for arboviral diagnosis","authors":"L. G. Morello, R. Rampazzo, A. Costa, M. Krieger, F. Marchini","doi":"10.35259/ISI.SACT.2019_32791","DOIUrl":"https://doi.org/10.35259/ISI.SACT.2019_32791","url":null,"abstract":"","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127793403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunossupression murine model to study antiviral resistance emergence during Influenza A infection","authors":"T. Silva","doi":"10.35259/ISI.SACT.2019_32862","DOIUrl":"https://doi.org/10.35259/ISI.SACT.2019_32862","url":null,"abstract":"Introduction: Influenza virus infection is a major cause of worldwide morbidity and mortality, affecting thousands of people annually. Severe cases are treated with viral Neuraminidase inhibitors like Tamiflu (Oseltamivir). However, high genetic variation could lead to emergence of antiviral resistant strains, limiting the treatment effectiveness. Antiviral-resistant viruses may arise spontaneously, by inappropriate antiviral use or by prolonged viral shedding observed in immunosuppressed patients.","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124392113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microsphere-couplings standardization with ZIKV or its E protein: The first step to construct a multiplex assay for Zika/Dengue","authors":"Stephanie Almeida, D. S. Matos, S. M. Lima, A. Souza, Thiago Santos Chaves, M. Medeiros, Leila Botelho, M. Pierre, P. Brasil, A. Azevedo","doi":"10.35259/isi.sact.2019_32804","DOIUrl":"https://doi.org/10.35259/isi.sact.2019_32804","url":null,"abstract":"Introduction: Zika virus (ZIKV) was considered world emergency during Zika outbreak in Brazil in 2015. ZIKV is a clinically important arbovirus that can cause fetal neurological complications in infected pregnant women and Guillain-Barré syndrome in adults. Serological diagnosis of ZIKV infection is a challenging due to high cross-reactivity between flaviviruses, for exemple with Dengue virus (DENV). Accurate and early diagnosis is essential to properly manage the patients. Samples collected in the viremic period of the ZIKV infection are conducted for viral load measurement by molecular tests and samples collected after that period are evaluated by serological tests such as ELISA and the results are confirmed by PRNT. In the present study, we intend to construct a diagnosis test based in the liquid microarray (LIM) to detect antibodies against ZIKV and DENV simultaneously, associated to treatment with a chaotropic agent step to minimize cross-reaction.","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"198 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116985383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of humoral response of individuals naturally exposed to malaria against synthetic and recombinant antigens representing Plasmodium vivax TRAP protein","authors":"Ada da Silva Matos, Rodrigo Silva, I. F. Soares, Barbara de Oliveira Baptista, P. Totino, C. Daniel-Ribeiro, C. Camacho, A. Reyes‐Sandoval, L. R. Pratt-Riccio, Josué da Costa Lima Junior","doi":"10.35259/isi.sact.2019_32533","DOIUrl":"https://doi.org/10.35259/isi.sact.2019_32533","url":null,"abstract":"Introduction: Thrombospondin-related adhesive protein (TRAP) of malaria parasites is essential for sporozoite motility and invasions into mosquito’s salivary gland and vertebrate’s hepatocyte. Due this importance, it is a promising target for pre-erythrocytic vaccine. Despite few reports on naturally acquired immune response against Plasmodium vivax TRAP (PvTRAP) are available, the results are conflicting and poorly explored in the Amazon region.","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"58 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122036471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Standardization of the xMAP Luminex methodology for simultaneous detection and differentiation of Dengue serotypes and Chikungunya viruses","authors":"M. Castro, L. Santana, J. Gonçalves, E. Pimenta, M. G. Cavalcanti, R. H. Peralta, J. M. Peralta","doi":"10.35259/isi.sact.2019_32775","DOIUrl":"https://doi.org/10.35259/isi.sact.2019_32775","url":null,"abstract":"Introduction: Arboviruses are among the most important emerging and reemerging virus in the world, transmitted mainly by the bite of the female mosquito of the genus Aedes. In Brazil, dengue virus (DENV) infection has been established since 1985, and more recently, Zika (ZIKV) and Chikungunya (CHIKV) virus infections have emerged as a public health concern. The clinical diagnosis of these arboviruses in the areas with DENV, CHIKV and ZIKV cocirculation become difficult due to the similarity of the signs and symptoms presented in these infections. Therefore, the search for a differential laboratory diagnosis is very important.","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125938504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Computational insights into the molecular interactions of anti-PD1/anti-DLL1 dual antibodies","authors":"Aline Albuquerque, J. Silva","doi":"10.35259/isi.sact.2019_32701","DOIUrl":"https://doi.org/10.35259/isi.sact.2019_32701","url":null,"abstract":"","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129369240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Computational identification of Coxiella burnetii non-conserved B-cell epitopes: a rational strategy for Q fever diagnosis","authors":"Thais Araujo, S. Fontes, T. Rozental, E. Lemos, P. C. Neves, Rodrigo Silva, F. Conte","doi":"10.35259/isi.sact.2019_32725","DOIUrl":"https://doi.org/10.35259/isi.sact.2019_32725","url":null,"abstract":"Introduction: Coxiella burnetii is the etiological agent of the zoonosis Q fever, a neglected disease which causes an acute or a chronic, life-threatening disease in humans. In its late course, Q fever can be complicated by fatal (eg, endocarditis) or debilitating (eg, chronic fatigue syndrome) disorders. Ruminants are considered as the main reservoirs for human infections but are usually asymptomatic or may manifest as late term abortions and therefore have significant economic impact. C. burnetii presents a highly stable and infectious cell form, which persists in the environment and is transmitted via inhalation of aerosols and/or consumption of contaminated milk. Q fever infection is most commonly diagnosed by serology tests but due to its impaired sensitivity, reliable clinic and veterinary diagnostic tests need to be developed. In this sense, recent immunoproteomic studies identified several proteins, which presented reactivity against sera from Q feverpatients, and are potential candidates for the development of new diagnostic tests. Objective: To identify, through a combination of prediction algorithms, immunogenic and non-conserved B-cell epitopes present on Coxiella burnetii immunoreactive proteins (outer membrane protein A, YAJc and LemA). Methodology: FASTA sequences of outer membrane protein A (ompA;CBU-1260), YAJc (yajC;CBU-1143) and LemA (lemA;CBU-0545) from C. burnetii were obtained from UNIPROT. The B-cell epitope prediction was carried out using two different algorithms, BepiPred and BCPred (threshold 0.5), whereas surface accessibility was predicted by Emini Surface Accessibility algorithm (threshold 1.0). We considered a potential B-cell epitope, sequences predicted by 2 or more algorithms that comprises, at least, 9 mers of length. Thereafter, the immunogenic potential from B-cell sequences were predicted by Vaxijen algorithm (threshold 0.4). The degree of sequence conservation between immunogenic B-cell epitopes and other C.burnetii related species (Francisella tularensis, Legionella pneumophila, Escherichia coli, Pseudomonas aeruginosa, Campylobacter jejuni, Rickettsia rickettsia, Ehrlichia chaffeensis, Bartonella henselae, Brucella melitensis, Afipia felis) were compared using protein BLAST databank. Results: Combining different prediction algorithms, we described four B-cell epitopes present on OMP-A (OMP-AE1, OMP-AE2, OMP-AE3 and OMP-AE4). OMP-AE1 and OMP-AE2 appears as two potential highly immunogenic B-cell candidates (Vaxijen score: 1.2 and 2.1, respectively), both with a low degree of sequence conservation (E-value >20), whereas OMP-AE3 and OMP-AE4 were predicted as B-cell sequences poorly immunogenic (Vaxijen score: 0.3 and 0.01, respectively). YAJc and LemA possess potential B-cell epitopes (YAJ-E01, LEM-E01 and LEM-E02; Vaxijen score: 1.2, 1.5 and 1.1, respectively), but although highly immunogenic, the predicted sequences are highly conserved (E-value < 1) among phylogenetic C. burnetii related species. Conclusio","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128915147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of technological roadmaps in shaping the future strategy for Bio-Manguinhos","authors":"Lethícia Mallet, R. Alves, Monique Menegaci, P. Barbosa, E. Gomes","doi":"10.35259/ISI.SACT.2019_32785","DOIUrl":"https://doi.org/10.35259/ISI.SACT.2019_32785","url":null,"abstract":"Introduction: The development of technological roadmaps has been a widely used technique in organizations to help map and manage the strategic portfolio that meet future market challenges. In particular, for organizations with high technological intensity, such as the pharmaceutical and biotechnology, roadmaps can help in the construction of articulated visions between demands defined by technological and market trends and the internal possibilities and resources of achieving them. Considering the review of Bio-Manguinhos’ Strategic Plan (2017-2028), Planning and Organization Advisory proposed the adoption of roadmapping technique to reliably support the executive board on analysis and selection of priority technological paths in the business areas of the Institute.","PeriodicalId":427855,"journal":{"name":"Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127899141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}