Standardization of the xMAP Luminex methodology for simultaneous detection and differentiation of Dengue serotypes and Chikungunya viruses

Abstract

Introduction: Arboviruses are among the most important emerging and reemerging virus in the world, transmitted mainly by the bite of the female mosquito of the genus Aedes. In Brazil, dengue virus (DENV) infection has been established since 1985, and more recently, Zika (ZIKV) and Chikungunya (CHIKV) virus infections have emerged as a public health concern. The clinical diagnosis of these arboviruses in the areas with DENV, CHIKV and ZIKV cocirculation become difficult due to the similarity of the signs and symptoms presented in these infections. Therefore, the search for a differential laboratory diagnosis is very important.