BioID法筛选免疫检查点淋巴细胞活化基因-3 (lag-3)相关蛋白

Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos Pub Date : 1900-01-01 DOI:10.35259/isi.sact.2019_32831

Priscila Ribeiro, L. Chicaybam, M. Pretti, M. Bonamino

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引用次数: 0

摘要

CAR - anti-CD20/Lag3 WT-BirA在HEK293T细胞中电穿孔，表达率为80%。Ep del和Kmut分别表达39%和41%的CAR。可以观察到CARs的细胞质定位。对CAR抗cd20 /Lag3WT-BirA进行Western blotting分析生物素化模式，观察到预期的阶梯生物素化模式。结论:构建的CARs在靶细胞系中表达，达到预期的生物素化模式。

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Screening of proteins related to the immunological checkpoint lymphocyte activation gene-3 (lag-3) through the BioID method

localization The CAR anti-CD20/Lag3 WT-BirA electroporated in the HEK293T cells presenting 80% of expression. CARs Ep del and Kmut showed 39% and 41% of CAR expression. it was possible to observe the cytoplasmatic localization of the CARs. Analysis of the biotinylation pattern by Western blotting was performed for CAR anti-CD20/Lag3WT-BirA, and the expected ladder pattern of biotinylation was observed. Conclusion : The constructed CARs were expressed in the target cell lines leading to the expected biotinilated patterns.

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Anais do IV International Symposium on Immunobiological e VII Seminário Anual Científico e Tecnológico de Bio-Manguinhos

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