Screening of proteins related to the immunological checkpoint lymphocyte activation gene-3 (lag-3) through the BioID method

Abstract

localization The CAR anti-CD20/Lag3 WT-BirA electroporated in the HEK293T cells presenting 80% of expression. CARs Ep del and Kmut showed 39% and 41% of CAR expression. it was possible to observe the cytoplasmatic localization of the CARs. Analysis of the biotinylation pattern by Western blotting was performed for CAR anti-CD20/Lag3WT-BirA, and the expected ladder pattern of biotinylation was observed. Conclusion : The constructed CARs were expressed in the target cell lines leading to the expected biotinilated patterns.